CN105533267A - Special phagostimulant for sepia pharaonis and application of special phagostimulant - Google Patents
Special phagostimulant for sepia pharaonis and application of special phagostimulant Download PDFInfo
- Publication number
- CN105533267A CN105533267A CN201510947476.0A CN201510947476A CN105533267A CN 105533267 A CN105533267 A CN 105533267A CN 201510947476 A CN201510947476 A CN 201510947476A CN 105533267 A CN105533267 A CN 105533267A
- Authority
- CN
- China
- Prior art keywords
- parts
- phagostimulant
- tiger spot
- feed
- spot cuttlefish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a special phagostimulant for sepia pharaonis and application of special phagostimulant. The special phagostimulant is characterized by being prepared from the following components in parts by weight: 2-8 parts of glycine, 3-8 parts of glutamic acid, 3-8 parts of alanine, 2-8 parts of asparagine, 5-10 parts of taurine, 10-20 parts of betaine, 10-15 parts of S-S-dimethyl-beta-bromo-propiothetin, 10-15 parts of stevioside, 5-10 parts of asafetida, 5-8 parts of citric acid, 5-10 parts of 1,2-double linoleic acid-Sn-glycerophosphoryl choline, 5-8 parts of IMP and 5-8 parts of UMP. The added mass percentage of the special phagostimulant in a sepia pharaonis compound feed is 2-3%. The special phagostimulant has the advantages that the utilization rate of the feed is increased, the weight increment rate and the survival rate of the sepia pharaonis are increased, and the feed coefficient and the feed dissolve-loss rate are reduced.
Description
Technical field
The present invention relates to the phagostimulant of a kind of cuttlefish, especially relate to a kind of tiger spot cuttlefish phagostimulant specially and application thereof.
Background technology
Tiger spot cuttlefish (Sepiapharaonis), belong to Mollusca (Mollusca), Cephalopoda (Cephalopoda), Coleoidea (Coleoidea), Sepiida (Sepioidea), Sepiidae (Sepiidae), Sepia (Sepia), for kind of dwelling at the bottom of the property of shallow sea, be distributed in the Indian Ocean-Western Pacific, in China, South Sea distribution is more.In breeding process, the juvenile stage of tiger spot cuttlefish is is still thrown something and fed the living body bait such as oppossum shrimp, shrimp, adult is is then thrown something and fed chilled small fish, novel mixed feed can be developed after considering, how to make tiger spot cuttlefish adapt to mixed feed fast and can ensure that again survival rate is then key point, therefore, need the growing state for tiger spot squid larva and adult, develop a kind of applicable phagostimulant, promote that tiger spot cuttlefish is to the peptic ulcer bleeding of man-made feeds, increases survival rate, cost-saving, reduce and pollute, increase the benefit.At present, any correlative study about tiger spot cuttlefish phagostimulant report is not also disclosed both at home and abroad.
Summary of the invention
Technical problem to be solved by this invention is to provide and a kind ofly improves efficiency of feed utilization, rate of body weight gain and increase survival rate, the tiger spot cuttlefish phagostimulant specially reducing feed coefficient and feed dissolve-loss ratio and application thereof.
The present invention solves the problems of the technologies described above adopted technical scheme:
1, a tiger spot cuttlefish phagostimulant specially, is made up of following component and parts by weight thereof: glycine 2-8, glutamic acid 3-8; alanine 3-8, asparagine 2-8, taurine 5-10; betaine 10-20, S-S-dimethyl-Beta-bromo DMPT 10-15, card is breathed out with glycosides 10-15; asafoetide 5-10; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 5-10 of citric acid 5-8,1,2-; adenylic acid (IMP) 5-8, uridylate (UMP) 5-8.
Preferably be made up of following component and parts by weight thereof: glycine 5, glutamic acid 5, alanine 5; asparagine 5, taurine 8, betaine 15; S-S-dimethyl-Beta-bromo DMPT 12; card is breathed out with glycosides 12, asafoetide 8, citric acid 6; 1; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 8 of 2-, adenylic acid (IMP) 6, uridylate (UMP) 6.
2, an application for tiger spot cuttlefish phagostimulant specially, the interpolation mass percent of above-mentioned tiger spot cuttlefish phagostimulant specially in mixed feed is 2-3%.
Compared with prior art, the invention has the advantages that: a kind of tiger spot cuttlefish phagostimulant specially of the present invention and application thereof, by glycine; glutamic acid, alanine, asparagine; taurine, betaine, S-S-dimethyl-Beta-bromo DMPT; card is breathed out with glycosides, asafoetide, citric acid; 1; the two sub-oleoyl-Sn-glyceryl phosphoryl choline of 2-, IMP, UMP by a certain percentage mixed configuration form.Ingest according to tiger spot cuttlefish and have a liking for and food use situation, about half neutral amino acid has feeding active, in acid amides, acidity and imino acid, only the effect of asparagine food calling is obvious, between above-mentioned neutral amino acid and asparagine, there is complementary characteristic, two kinds or above combination ingesting advantageously in tiger spot cuttlefish.Add taurine, be beneficial to the lymphocytic growth and breeding of tiger spot cuttlefish immune system, tiger spot cuttlefish can be made to increase weight obviously; Betaine, as a kind of quaternary ammonium type alkaloid, as methyl donor, has and promotes fat metabolism, mitigation stress reaction, adjustment osmotic pressure, stable vitamin, raising efficiency of feed utilization; S-S-dimethyl-Beta-bromo DMPT has another name called bromination carboxyethyl dimethyl gold-plating, it is a kind of sulfur-containing compound be enriched in phytoplankton, marine alga and mollusk, to tiger spot cuttlefish, there is strong food calling and growth promoting function, strengthen its endurance to hydro-environmental change, simultaneously or the source of methyl needed for cuttlefish Nutrition and Metabolism; Carbohydrate is very big to the feeding active of aquatic livestock, can think without encumbering effect, and wherein the glycocide card of sugariness vegetable crude drug is breathed out with glycosides is exactly a kind of representative carbohydrate, and it has short activity of ingesting to tiger spot cuttlefish, and attraction power increases with concentration and strengthens; Asafoetide is because having special odor containing volatility low molecule organic matter, and its strong garlic perfume has spread effect to the sense of smell of tiger spot cuttlefish, can attract cuttlefish to ingest; Organic acid has good phagostimulating effect to aquatic livestock, and in the single nerve fibre of wherein aquatic livestock, some fiber responds to citric acid, growth of can exciting nerve.1, the two sub-oleoyl-Sn-glyceryl phosphoryl choline of 2-is as a kind of fat, it is the carrier of nutritional labeling and liposoluble vitamin, the molecular activity of molecular proportion only containing saturated fatty acid containing unrighted acid is high, suitable n-3 or n-6 unrighted acid, ensure that n-3 unrighted acid level in muscle, the feed nutrition index of n-3 unrighted acid can not be caused to decline, survival rate and output can be significantly improved.Under normal circumstances, the nucleotide pair of synthesis in body is enough in maintenance normal growth, and the extra effect of adding extraneous nucleotide and having growth promoting function and strengthening immunity, therefore IMP and UMP has good growth-promoting effect as nucleotide pair tiger spot cuttlefish.This tiger spot cuttlefish phagostimulant specially, improves tiger spot cuttlefish efficiency of feed utilization, rate of body weight gain and survival rate, and significantly accelerate the Feeding Velocity of tiger spot cuttlefish, feed coefficient also all has reduction, and feed dissolve-loss ratio reduces.
Show through inventor's experimental result, under the coordinated effect of each composition, tiger spot cuttlefish phagostimulant specially of the present invention adds in commercially available business mixed feed by the dosage of mass percent 2-3%, with do not add compared with mixed feed of the present invention, after this phagostimulant of throwing something and feeding, the survival rate of tiger spot cuttlefish adds 8.96%-29.09%, efficiency of feed utilization adds 18.52%-41.94%, rate of body weight gain adds 317.53%-464.48%, and feed coefficient reduces 0.2-0.4, and feed dissolve-loss ratio reduces 1.7%-5.8%.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
One, specific embodiment
A kind of tiger spot cuttlefish phagostimulant specially, is made up of following component and parts by weight thereof: glycine 2-8, glutamic acid 3-8; alanine 3-8, asparagine 2-8, taurine 5-10; betaine 10-20, S-S-dimethyl-Beta-bromo DMPT 10-15, card is breathed out with glycosides 10-15; asafoetide 5-10; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 5-10 of citric acid 5-8,1,2-; IMP5-8, UMP5-8.
Embodiment 1
A kind of tiger spot cuttlefish phagostimulant specially, is made up of following component and parts by weight thereof: glycine 5, glutamic acid 5; alanine 5, asparagine 5, taurine 8; betaine 15, S-S-dimethyl-Beta-bromo DMPT 12, card is breathed out with glycosides 12; asafoetide 8; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 8 of citric acid 6,1,2-; IMP6, UMP6.
Embodiment 2
A kind of tiger spot cuttlefish phagostimulant specially, is made up of following component and parts by weight thereof: glycine 2, glutamic acid 3; alanine 3, asparagine 2, taurine 5; betaine 10, S-S-dimethyl-Beta-bromo DMPT 10, card is breathed out with glycosides 10; asafoetide 5; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 5 of citric acid 5,1,2-; IMP5, UMP5.
Embodiment 3
A kind of tiger spot cuttlefish phagostimulant specially, is made up of following component and parts by weight thereof: glycine 8, glutamic acid 8; alanine 8, asparagine 8, taurine 10; betaine 20, S-S-dimethyl-Beta-bromo DMPT 15, card is breathed out with glycosides 15; asafoetide 10; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 10 of citric acid 8,1,2-; IMP8, UMP8.
Embodiment 4
A kind of tiger spot cuttlefish phagostimulant specially, is made up of following component and parts by weight thereof: glycine 4, glutamic acid 4; alanine 7, asparagine 7, taurine 6; betaine 18, S-S-dimethyl-Beta-bromo DMPT 12, card is breathed out with glycosides 13; asafoetide 7; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 9 of citric acid 6,1,2-; IMP6, UMP6.
Embodiment 5
A kind of tiger spot cuttlefish phagostimulant specially, is made up of following component and parts by weight thereof: glycine 3, glutamic acid 7; alanine 4, asparagine 4, taurine 6; betaine 12, S-S-dimethyl-Beta-bromo DMPT 14, card is breathed out with glycosides 11; asafoetide 6; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 6 of citric acid 7,1,2-; IMP7, UMP7.
Two, Application Example
Test site: in the culturing pool of Fenghua, Ningbo City plant, culturing pool standard is that 3m × 4m × 1.5m(is long × wide × high).
Breed variety: the tiger spot squid larva adopting aquatic products research institute of Zhejiang Province base to hatch out, selects the individuality that size is roughly the same with weight.
Experimental design: arrange 4 test group, wherein test group 4 is blank groups, and each test group 3 repetition, each cement pit cultivates 40 tiger spot cuttlefishes.
Aquaculture management: the water Inversion phenomenon of seawater, pH value keep substantially identical, the tiger spot cuttlefish basis mixed feed of throwing something and feeding is the same, be made up of following component and parts by weight thereof: mixed feed is formulated by the raw material Homogeneous phase mixing of following parts by weight: white fish meal 30, krill meal 15, seawood meal 2, fermented bean dregs 15, Choline Chloride 5, alphalise starch 10, dextrin 10, the single polyphosphate 2 of vitamin C-2-, calcium dihydrogen phosphate 2, lecithin 2, fish oil 5, flour 20 (or directly the cuttlefish mixed feed sold of market).Difference is the phagostimulant that with the addition of in the ratio of 2.5wt% in experimental group 1 feed of to throw something and feed every day in embodiment 1; With the addition of the phagostimulant in embodiment 2 in the ratio of 2.5wt% in the feed that experimental group 2 is thrown something and fed every day; With the addition of the phagostimulant in embodiment 3 in the ratio of 2.5wt% in the feed that experimental group 3 is thrown something and fed every day.Any phagostimulant is not added in the mixed feed that experimental group 4 is thrown something and fed every day.Day feeds intake twice, and the every day of 0 ~ 45 age in days changes water once, quantity of exchanged water 50% ~ 80%, changes the front diddle-net of water and drags for the residual bait in bottom and foul, fall pond once every 10d; After reaching 45 ages in days, change water twice every day, quantity of exchanged water 90%-100%, adopt the siphonage removing residual bait in bottom and booty, fall pond once every 20 ~ 30d.Every day records the forage volume of throwing something and feeding.The addition of above-mentioned tiger spot cuttlefish phagostimulant specially in the mixed feed of tiger spot cuttlefish basis also can be 2% or 3%.The tiger spot cuttlefish die will be cleaned out every day in above-mentioned cement pit.
Results contrast: after cultivating 12 weeks, counts the number of tiger spot cuttlefish in each cement pit, is calculated to be motility rate, weighs to the tiger spot cuttlefish in each cement pit simultaneously, calculates rate of body weight gain, efficiency of feed utilization.Result of the test is as shown in table 1, as can be seen from Table 1, experimental group 1, 2, 3 three groups compare with experimental group 4, survival rate, rate of body weight gain and efficiency of feed utilization, there were significant differences (p < 0.05), experimental group 1, 2, survival rate between 3 and rate of body weight gain are without significant difference (p > 0.05), significant difference (p < 0.05) is there is between efficiency of feed utilization, the efficiency of feed utilization of experimental group 1 is the highest, mean value reaches 68.8%, as can be seen here, the phagostimulant prepared according to this case method adds in tiger spot cuttlefish mixed feed, the survival rate of tiger spot cuttlefish can be significantly improved, rate of body weight gain and efficiency of feed utilization (the results are shown in Table shown in 1).
Table 1 is thrown something and fed tiger spot cuttlefish indices after feed
From above-mentioned table 1, after adding tiger spot cuttlefish phagostimulant specially, the survival rate of tiger spot cuttlefish adds 8.96%-29.09%, and efficiency of feed utilization adds 18.52%-41.94%, and rate of body weight gain adds 317.53%-464.48%, feed coefficient reduces 0.2-0.4, and feed dissolve-loss ratio reduces 1.7%-5.8%.
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to above-mentioned citing.Those skilled in the art are in essential scope of the present invention, and the change made, remodeling, interpolation or replacement, also should belong to protection scope of the present invention.
Claims (3)
1. a tiger spot cuttlefish phagostimulant specially, is characterized in that being made up of following component and parts by weight thereof: glycine 2-8, glutamic acid 3-8; alanine 3-8, asparagine 2-8, taurine 5-10; betaine 10-20, S-S-dimethyl-Beta-bromo DMPT 10-15, card is breathed out with glycosides 10-15; asafoetide 5-10; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 5-10 of citric acid 5-8,1,2-; adenylic acid 5-8, uridylate 5-8.
2. a kind of tiger spot cuttlefish phagostimulant specially according to claim 1, is characterized in that being made up of following component and parts by weight thereof: glycine 5, glutamic acid 5; alanine 5, asparagine 5, taurine 8; betaine 15, S-S-dimethyl-Beta-bromo DMPT 12, card is breathed out with glycosides 12; asafoetide 8; the two sub-oleoyl-Sn-glyceryl phosphoryl choline 8 of citric acid 6,1,2-; adenylic acid 6, uridylate 6.
3. an application for tiger spot cuttlefish phagostimulant specially according to claim 1, is characterized in that: the interpolation mass percent of tiger spot cuttlefish phagostimulant specially according to claim 1 in mixed feed is 2-3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510947476.0A CN105533267A (en) | 2015-12-17 | 2015-12-17 | Special phagostimulant for sepia pharaonis and application of special phagostimulant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510947476.0A CN105533267A (en) | 2015-12-17 | 2015-12-17 | Special phagostimulant for sepia pharaonis and application of special phagostimulant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105533267A true CN105533267A (en) | 2016-05-04 |
Family
ID=55813267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510947476.0A Pending CN105533267A (en) | 2015-12-17 | 2015-12-17 | Special phagostimulant for sepia pharaonis and application of special phagostimulant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105533267A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762997A (en) * | 2005-10-18 | 2006-04-26 | 北京格林富诚科技有限公司 | Dialkyl-beta-propiothetin haloid acid salt and carboxylate preparation method |
| CN101401621A (en) * | 2008-10-21 | 2009-04-08 | 浙江海洋学院 | Spotted maigre young postlarva feeding promoting agent and method of preparing the same |
| CN101491225A (en) * | 2009-02-19 | 2009-07-29 | 浙江海洋学院 | Rearing method capable of increasing cuttlebone medical value of Sepiella maindroni |
| CN101756025A (en) * | 2008-12-11 | 2010-06-30 | 天津瑞贝特科技发展有限公司 | Food attractant-growth promoting oral administration nutritional composition for aquiculture animal. and preparation method and application thereof |
| CN102232488A (en) * | 2011-06-24 | 2011-11-09 | 浙江海洋学院 | Sepiella maindroni basal bait formula |
| CN102487867A (en) * | 2011-12-07 | 2012-06-13 | 中国水产科学研究院黄海水产研究所 | Mixed culture method for sepia esculenta offspring seed and cynoglossus semilaevis offspring seed |
| CN102630833A (en) * | 2012-04-25 | 2012-08-15 | 珠海海一水产饲料有限公司 | Phagostimulant for raw fish feed |
| CN102669434A (en) * | 2012-06-05 | 2012-09-19 | 广东海大集团股份有限公司 | Jewfish feed phagostimulant and preparation method thereof |
| CN103004717A (en) * | 2012-12-13 | 2013-04-03 | 李群益 | Controlled release bait material and preparation method thereof |
| CN104206822A (en) * | 2014-08-08 | 2014-12-17 | 广东海大集团股份有限公司 | Efficient compound attractant growth promoting additive and preparation and application thereof |
| CN104522409A (en) * | 2014-12-30 | 2015-04-22 | 青岛七好营养科技有限公司 | Feeding partner for mink pellet feed and preparation method of feeding partner |
| CN105104905A (en) * | 2015-10-12 | 2015-12-02 | 岭南师范学院 | Formula of feed for sepia lycidas |
-
2015
- 2015-12-17 CN CN201510947476.0A patent/CN105533267A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762997A (en) * | 2005-10-18 | 2006-04-26 | 北京格林富诚科技有限公司 | Dialkyl-beta-propiothetin haloid acid salt and carboxylate preparation method |
| CN101401621A (en) * | 2008-10-21 | 2009-04-08 | 浙江海洋学院 | Spotted maigre young postlarva feeding promoting agent and method of preparing the same |
| CN101756025A (en) * | 2008-12-11 | 2010-06-30 | 天津瑞贝特科技发展有限公司 | Food attractant-growth promoting oral administration nutritional composition for aquiculture animal. and preparation method and application thereof |
| CN101491225A (en) * | 2009-02-19 | 2009-07-29 | 浙江海洋学院 | Rearing method capable of increasing cuttlebone medical value of Sepiella maindroni |
| CN102232488A (en) * | 2011-06-24 | 2011-11-09 | 浙江海洋学院 | Sepiella maindroni basal bait formula |
| CN102487867A (en) * | 2011-12-07 | 2012-06-13 | 中国水产科学研究院黄海水产研究所 | Mixed culture method for sepia esculenta offspring seed and cynoglossus semilaevis offspring seed |
| CN102630833A (en) * | 2012-04-25 | 2012-08-15 | 珠海海一水产饲料有限公司 | Phagostimulant for raw fish feed |
| CN102669434A (en) * | 2012-06-05 | 2012-09-19 | 广东海大集团股份有限公司 | Jewfish feed phagostimulant and preparation method thereof |
| CN103004717A (en) * | 2012-12-13 | 2013-04-03 | 李群益 | Controlled release bait material and preparation method thereof |
| CN104206822A (en) * | 2014-08-08 | 2014-12-17 | 广东海大集团股份有限公司 | Efficient compound attractant growth promoting additive and preparation and application thereof |
| CN104522409A (en) * | 2014-12-30 | 2015-04-22 | 青岛七好营养科技有限公司 | Feeding partner for mink pellet feed and preparation method of feeding partner |
| CN105104905A (en) * | 2015-10-12 | 2015-12-02 | 岭南师范学院 | Formula of feed for sepia lycidas |
Non-Patent Citations (1)
| Title |
|---|
| 张静: "S,S-二甲基 - β- 溴代丙酸噻亭作为动物诱食剂的研究和应用新进展", 《化工中间体》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103355491A (en) | Alga immunopotentiation compound feed for litopenaeus vannamei | |
| CN105410451A (en) | Spartina alterniflora compound feed for Chinese mitten crabs | |
| AU2015201193B2 (en) | Feed product and/or feed ingredient | |
| US20170347684A1 (en) | Natural and sustainable seaweed formula that replaces synthetic additives in swine feed | |
| Sriputhorn et al. | Fairy shrimp (Streptocephalus sirindhornae) as live feed improve growth and carotenoid contents of giant freshwater prawn Macrobrachium rosenbergii | |
| CN104543407B (en) | It is a kind of to promote mandarin fish to ingest the compound additives of man-made feeds | |
| CN102283337B (en) | Grown chicken feed | |
| CN105285490A (en) | Compound powdered feed for anguilla anguilla in the adult-stage | |
| CN102648737A (en) | Macrobrachium rosenbergii fish-meal-free compound feed | |
| CN103875920A (en) | Large yellow croaker feed additive with intestinal tract protection effect and preparation method and application thereof | |
| CN104171369A (en) | Mulberry leaf powder and mulberry twig and ganoderma lucidum leftover powder added feed for livestock and birds and application thereof | |
| Natsir et al. | Utilization of Caulerpa sp. as a feed ingredient for growth and survival of whiteleg shrimp and Chanos chanos in polyculture | |
| CN104304750A (en) | Green seaweed compound feed for penaeus monodon | |
| CN103636947B (en) | For feed addictive and the production method thereof of high protein, the aquatic product matched feed of low fish meal | |
| Gao et al. | Microalgae as fishmeal alternatives in aquaculture: current status, existing problems, and possible solutions | |
| KR101481484B1 (en) | Feed for abalon | |
| CN102265994B (en) | Glass eel compound feed | |
| JP2011200260A (en) | Feed for fish of thunnus | |
| CN105595051A (en) | Specific phagostimulant for pomfret and application thereof | |
| CN105533267A (en) | Special phagostimulant for sepia pharaonis and application of special phagostimulant | |
| CN104304799B (en) | Glass eel later stage mixed feed | |
| CN103750033A (en) | Prawn feed additive with intestinal tract protection effect as well as preparation method and application thereof | |
| KR20150066227A (en) | Feed composition comprising jade, mineral, seaweed, Neatus ventralis and Bacillus sp. for sea cucumber and preparing method thereof | |
| Komilus et al. | Dried acetes as growth promoter for guppy (Poecilia reticulata) nutrition | |
| KR20170058215A (en) | Complete formular feed using yellow earth for juvenile sea cucumber production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160504 |