KR20150066227A - Feed composition comprising jade, mineral, seaweed, Neatus ventralis and Bacillus sp. for sea cucumber and preparing method thereof - Google Patents

Abstract

The present invention relates to a feed composition which can be used for effectively culturing or cultivating sea cucumbers, and a method for manufacturing the same. According to the present invention, the composition for sea cucumber feed includes inexpensive jade powder instead of mud and thus can induce sea cucumbers to adapt to the environment by helping diet and growth of the sea cucumbers; includes inexpensive Neatus ventralis powder with high protein derived from animals, functions as a protein source, instead of fish powder, for the sea cucumbers, promotes growth of the sea cucumbers, and provides enough nutrients to the sea cucumbers; and includes Bacillus bacteria as a probiotic agent and thus can induce the sea cucumbers to actually decompose and absorb the Neatus ventralis powder. Therefore, the composition for sea cucumber feed of the present invention can outstandingly increase growth of the sea cucumbers and can effectively reduce mortality. Especially, the composition for sea cucumber feed includes jade powder, minerals, seaweed, Neatus ventralis powder, and Bacillus bacteria at an optimal ratio and thus can be advantageously used for a sea cucumber cultivation industry. Furthermore, the composition of the present invention is expected to substitute imported raw materials in general, to contribute with low production costs to an industrial infrastructure, to promote a domestic sea cucumber cultivation industry, and to increase national competitiveness.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for feed of sea cucumber including an active ingredient of jade, a mineral, a seaweed, a gourd, and a bacillus, and a method for producing the same. for sea cucumber and preparing method thereof}

The present invention relates to a feed composition which can be used for the effective cultivation or cultivation of sea cucumber and a method of producing the same.

There are more than 1,500 species of sea cucumbers in the world. The sea cucumbers in Northeast Asia Far East Asia are classified into the protruding sea cucumbers, which are the keywords of classification, and the other sea cucumbers. In Korea, the protruding sea cucumbers are mainly cultured. China has already developed its industry rapidly in relation to the food culture of Boryeong for over 60 years, and Japan has led various industrial industries in abalone. This kind of cultural culture has developed industries such as Jongmyo, Middle cultivation, Sungsam, and Hyangsam in the aquaculture industry and led various supplementary industries.

In Korea, sea cucumber cultivation has also begun for over 10 years. As a part of China 's food processing industry, dried fish ginseng has been traded at a high price, and industrial trends have come through artificial cultivation rather than natural harvesting. However, the absence of technology and the lack of industrial infrastructure have prevented the sea cucumber farming industry from spreading. In addition, it is a domestic phenomenon that depends only on regional discharge, and it is not even attempted in the mid-upbringing or sex culture. For industrial development beyond discharge, economic fisheries management is needed. It is urgent to develop mid to low-priced feeds that can help to manage such fisheries.

Although the composition of the sea cucumber diet varies, it is generally accepted that nutrients are supplemented mainly by algae and animal proteins rich in mud and vegetable proteins. However, in Korea, the development of sea cucumber feeds is still insufficient and there is no market, so the industry is not spreading. Because of this technical and industrial base is insufficient, most of the domestic sea cucumber feeds are imported and supplied from China.

In the sea cucumber culture, mud has a very important meaning. Like the sea cucumbers, living and living in the lowlands live by eating the degradation products in the lowlands. At that time, it is the sea cucumber that has a unique structure that absorbs the useful substances through the mud and emits the mud again. mud) is a very important raw material for digestion and growth. In addition, it can induce the metabolism more actively through the inflow of minerals that help the metabolism in the body, such as anion or far-infrared rays. Especially, when the absorbency according to the particle size is added, it can induce rapid growth.

Sea cucumber is an adherent animal, but in the beginning, the habitat environment is considered to be a very important vegetable protein source such as seaweed and sea tangle, brown algae and worms (sea moss, mamizo). In the early stage of breeding, these vegetable proteins have been added to help to adapt to adaptation to the growing environment (adherent chondropan). DMPT (dimethyl-β-propiothetin) from this report suggests that sea cucumbers help to attach to the wafers. These useful materials have provided considerable help. In addition, when sea cucumber grows to a certain size, it exhibits body color and exhibits increased disease resistance and rapid growth of the body. Since then, animal proteins are required. At this time, crude protein can induce growth by using high raw materials. The addition of good animal protein can also aid in the growth of sea cucumbers.

As a precedent literature on cetacean feeds, Korean Patent Laid-Open Publication No. 10-2005-0062763, entitled " Method of producing sea cucumber feedstuffs ", shows that the larvae of sea cucumbers as main feedstuffs are marine algae such as seaweed, Etc., and then dissolving in water to provide a celestial feed in the form of a juice. In Korean Patent Laid-Open Publication No. 10-2006-0125455 entitled " A method of providing a sea cucumber feed in the form of a juice by dissolving in seaweeds such as seaweed, sea tangle and mackerel, and a granular form including fish meal, soybean curd and blood meal, and disclosed in Korean Patent No. 10-0915848, , Which has improved digestion and absorption rate and palatability, is disclosed as a feed additive for sea cucumber which contains herbal supplements, dandruff, liquorice, licorice root, Angelica gigas, and rhododendron as effective ingredients.

However, the composition of the sea cucumber which is proposed in the above literature has a problem in that the sea cucumbers have problems in efficiently ingesting and extinguishing the sea cucumbers, and the cost of the components constituting the composition of the feed is high, There is no disadvantage.

Accordingly, the present inventors have made efforts to develop eco-friendly sea cucumber diets while overcoming the above-mentioned ingestion, digestion efficiency, and economical efficiency of the feed, and as a result, The present inventors have completed the present invention by confirming that the feed composition can effectively increase the survival rate and growth rate of sea cucumber and significantly reduce mortality. Particularly, fishmeal as a protein and nutrient source of marine biomass feed is a major cause of increasing feed ratio. The present inventor tried to utilize insect resources rich in high protein and nutrients as a substitute for such fishmeal, And the optimum mixing ratio (composition ratio) according to the application of the sea cucumber feed was confirmed, thereby completing the present invention.

Korean Patent Publication No. 10-2013-0009425 Korean Patent Laid-Open No. 10-2009-0070160

Accordingly, an object of the present invention is to provide an economical and eco-friendly sea cucumber dietary composition which not only increases the efficiency of ingestion and digestion of diets, but also increases the growth and survival rate of sea cucumbers.

Another object of the present invention is to provide a method for producing the composition.

In order to accomplish the object of the present invention as described above, the present invention provides a composition for feed of sea cucumber comprising an oak powder, a mineral, a seaweed powder, a gourd powder and a bacillus germ as an active ingredient.

In one embodiment of the present invention, the mineral may be zeolite.

The In one embodiment, the seaweed may be at least one selected from the group consisting of seaweed, kelp and sea weed.

In one embodiment of the present invention, The composition may include 30 to 60 parts by weight of the oak powder, 10 to 30 parts by weight of the mineral, 5 to 25 parts by weight of the seaweed, 5 to 10 parts by weight of the gruel powder and 1 to 5 parts by weight of the bacterium.

Also, the present invention provides a method for preparing a fine particle, comprising the steps of: (a) preparing each of a jade powder, a mineral, a seaweed, and a gravel powder to a size of 500 to 100 mesh; And (b) adding Bacillus bacteria to the prepared jade powders, minerals, seaweeds and gourd powders and mixing them.

In one embodiment of the present invention, the mineral may be zeolite.

In one embodiment of the present invention, the seaweed may be at least one species selected from the group consisting of seaweed, kelp and sea weed.

In one embodiment of the present invention, the composition ratio of the respective components to be mixed in the step (b) is 30 to 60 parts by weight of the oak powder, 10 to 30 parts by weight of the mineral, 5 to 25 parts by weight of the seaweed, 5 to 10 parts by weight of the powder and 1 to 5 parts by weight of the bacterium.

The composition for feed of sea cucumber according to the present invention can induce adaptation to the environment by helping feeding and growing of sea cucumber including oak powder which is cheaper in price instead of mud; It is possible to provide sufficient nutrients in addition to growth of fish celia as a protein source of sea cucumber in place of fish meal by including an animal high protein but low cost chewing gum powder; It is possible to induce the sea cucumber to actually dissolve and absorb the germanium powder including the bacillus as a probiotic agent. The composition of the present invention including all of them can remarkably increase the growth of sea cucumber and effectively reduce mortality have. In particular, the composition for feed of sea cucumber according to the present invention can be advantageously used in the sea cucumber culture industry by deriving the optimal composition ratio of jade powder, mineral, algae, gourd powder and Bacillus bacteria. Furthermore, it is expected that the present invention will provide an industrial basis for reducing the import substitution effect and the unit price of the whole raw materials, and it will be possible to activate the domestic sea cucumber aquaculture industry and enhance the national competitiveness.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram briefly showing an operation mechanism of Bacillus bacteria as a feed additive.
2 is a photograph showing each raw material used in the composition for feed of sea cucumber of the present invention.
FIG. 3 is a photograph showing the sides (thin state) of the sea cucumber after feeding of the composition for feed of the sea cucumber of the present invention.
4 is a photograph showing a nutrient, a vitamin ingredient used in the experiment of the present invention, and a conventional feed (sea cucumber feed) used as a control group.

In one aspect, the present invention relates to a composition for feed of sea cucumber comprising as an active ingredient an oak powder, a mineral substance, seaweed powder, gourd powder and bacillus bacteria.

The composition of the present invention can contain an oak powder as an active ingredient and the oak powder replaces mud which is an important raw material for digestion and growth of sea cucumber. In general, species living and living in lowlands such as sea cucumbers live by eating decomposition products in lowlands. At that time, they have a unique structure that absorbs useful substances through mud, .

Therefore, in the composition of the present invention, the oak powder plays a role of inducing adaptation to the environment by helping the feeding and growth of sea cucumber.

In one embodiment of the present invention, the kind of the jade is not particularly limited, such as jade jade, jadeite jade, jade jade jade, jade jade, jade jade, jade jade, jade jade, etc. In the following examples, Were used.

In addition, the composition of the present invention can contain minerals as an active ingredient, and the minerals can more actively induce metabolism through the release of anions or far-infrared rays, and in particular, Of the total population.

In one embodiment of the present invention, the mineral is not particularly limited as long as it can induce metabolism by the release of anions or far-infrared rays, but zeolite is used in the following examples.

In addition, the composition of the present invention can contain seaweed as an active ingredient. The seaweeds are rich in vegetable protein and various nutrients, and have a major role of reinforcing nutrition in sea cucumbers, And it helps to adapt to fit.

In one embodiment of the present invention, the seafood may be at least one species selected from the group consisting of seaweed, kelp and sea weed. In the following examples, seaweed, kelp and sea weed are all mixed.

In addition, the composition of the present invention for the feed of sea cucumber may contain gummy powder as an effective ingredient, and the gummy gland powder plays a role of a fish meal corresponding to a main protein source in the sea cucumber culture.

In general, fish meal, which is the main protein material of feed for fish farming or marine biomass feed, is in a trend of surging recently due to surplus demand. Therefore, it has a similar or improved nutritional value compared to fish meal Demand is urgent.

Accordingly, in response to the above-mentioned demand, the present invention replaces the high protein and nutrient-rich insect resources, and the beetle, which is a larva of beetle, is selected as a kind of insect.

In addition, the composition for feed of the present invention can contain Bacillus bacteria as an effective ingredient, and the Bacillus bacteria act as a probiotic for promoting food digestion and absorption of sea cucumber.

The term " probiotic agent " of the present invention refers to a preparation (including a fermentation product) containing live beneficial microorganisms, which is fixed in the intestines of an animal and inhibits the growth of harmful microorganisms through competitive exclusion with other pathogenic microorganisms, , Which promotes the growth of animals and improves feed efficiency. It is now known as probiotics or direct-fed microbials (DFM).

By inducing "Bacillus (Bacillus. Sp) 'it is a growth-promoting with particular suit (easy to give chapter) effect, promote digestion effect, feeding the effect into one of the probiotics most commonly used since 2000 as a representative strains of probiotics It is the most useful microbial strain (see Fig. 1). These bacillus also excrete the action in the body to decompose nitrogen (N) and phosphorus (P), which are water pollutants, to stabilize the water system environment.

In particular, the Bacillus bacterium of the present invention can act to induce the sea cucumbers to actually dissolve and absorb the gully powder.

In addition, the composition for feed of the present invention may further comprise other substances necessary for growth and survival of sea cucumbers in addition to the above-mentioned active ingredients (jade powder, mineral substances, seaweeds, gourd powder and Bacillus bacteria) , Nutrients and vitamins were added in addition to the active ingredient.

The composition for feed of sea cucumber according to the present invention can remarkably increase the growth of sea cucumber and effectively reduce the mortality by containing all of the above-mentioned jade powder, minerals, seaweeds, gourd powder and Bacillus bacteria as effective ingredients.

In the following Example 1 of the present invention, as a result of experiment for promoting the growth of the sea cucumber and reducing the mortality of the sea cucumber according to the composition feed of the sea cucumber, the composition of the present invention for sea cucumber , The body weight of the sea cucumber was increased and the mortality rate was significantly decreased (see Tables 2 and 3 below). In addition, it was observed that the experimental group fed with the sea cucumber diets of the present invention was observed, and it was found that the metabolites produced in the metabolic process were also thinly and constantly discharged, so that the continuous metabolism was quite uniform (see FIG. 3).

In an embodiment of the present invention, the bait crayfish composition comprises 30 to 60 parts by weight of a jade powder, 10 to 30 parts by weight of a mineral, 5 to 25 parts by weight of seaweed, 5 to 10 parts by weight of a gourd powder, To 5 parts by weight.

Particularly, it is preferable that the gypsum feed composition according to the present invention contains 5 to 10 parts by weight of the gypsum powder, and when the gypsum powder is less than 5 parts by weight based on the total weight of the composition, If it contains more than 1 part by weight, sufficient digestion due to decomposition and reduction of digestibility is reduced.

In the following Example 2 of the present invention, for the purpose of analyzing the optimal composition ratio of feed composition to maximize growth and mortality of sea cucumber, the body length and mortality of ginseng and ginseng were investigated according to the addition ratio of gourd powder as a protein source As a result, it was confirmed that the growth and survival rate of sea cucumber were significantly higher in the experimental group 2 containing 8% by weight of the dough powder. Thus, it was confirmed that the sea cucumbers having the optimum mixing ratio, Experimental group 2 was the most suitable for the composition of feed composition, and as a result of integration with Example 1, it was judged that blending of 5 to 10% by weight of gruel powder in the feed was most effective for growth of sea cucumber See Table 5 and Table 6).

According to another aspect of the present invention, there is provided a method of manufacturing a honeycomb structure, comprising the steps of: (a) pulverizing each of jade powder, mineral, algae and gravel powder to a size of 500 to 100 mesh; And (b) adding a Bacillus bacterium to the prepared jade powders, minerals, seaweeds and gourd powders and mixing them.

In one example of the present invention, the mineral may be zeolite, and the seaweed may be at least one selected from the group consisting of seaweed, kelp and sea weed.

In another embodiment of the present invention, the composition ratio of the respective components mixed in the step (b) is 30 to 60 parts by weight of the oak powder, 10 to 30 parts by weight of the mineral, 5 to 25 parts by weight of the seaweed, 5 to 10 parts by weight of ginger powder and 1 to 5 parts by weight of Bacillus cereus.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example >

Where to buy each raw material used in the experiment

The mud was purchased in Sinan County, Jeollanam-do. The zeolite was purchased from a Busan handling company. The Suam Ok is imported directly from the head office. The Seaweed Powder, Kelp Powder, Nutrient, (See Fig. 4), and the dough was purchased and prepared at the farm of the Chungnam sub-station (the dry dough) Were purchased and used in powder form). The Bacillus used in the experiment was Bacillus Polyfermenticus and B. lichenoiformis were used. The existing feeds were categorized as the finished products of the Yantai Dairy Feedstuff Co., Ltd., a manufacturer of sea cucumber only (one kind of celery root [0.2 ~ 1 cm or less] and one kind of sea cucumber [1 ~ 5cm] , And this feed is a feedstuff containing no mildew.

< Example  1>

Analysis of body weight and mortality according to the feeding of compound feed for sea cucumber of the present invention

The present inventors first measured the mortality and growth test using ginseng and ginseng to test the growth promotion and mortality of the sea cucumber according to the feeding of the compounded feed for sea cucumber of the present invention. For the production of feeds, all raw materials were ground to a size of over 500 mesh (in the case of a goose, the dry dough purchased from the direct cultivation farm of the Chungnam subdistrict was prepared in powder form). For the growth promotion experiment of sea cucumber, (See Table 1 below). Each feedstock was first mixed with a feeder manufacturer's mixer, and after sufficient mixing for 30 minutes, the feedstuffs were prepared, and feeds were prepared according to the conditions, and the same amount was fed twice per day to each group Respectively.

The experiment was divided into two groups: ginseng (0.2 ~ 1 cm) and ginseng (1 ~ 5cm). 100 ginseng (0.9 g of ginseng and 4.5 ± 1.2 g of ginseng) were used. The feedstuffs were divided into two groups: the control group 1, which contained no mud, and the control group 2, which contained 30% mud, and the compound feed of sea cucumber of the present invention was used as the experimental group. The mortality rate was measured and the total duration of the experiment was 3 months. The temperature was set so that the constant temperature of the sea cucumber was kept constant at 16 ° C. Statistical significance was tested by ANOVA-test.

Mixed feed for sea cucumber Control 1 100% by weight of conventional feed (no added) Control group 2 70% by weight of conventional feed, 30% by weight of mud, Experimental group 5% by weight of seaweed, 5% by weight of seaweed, 10% by weight of worms, 10% by weight of ginger powder, 2% by weight of bacillus bacteria, 5% by weight of nutrients, 3% by weight of vitamins,

Body weight and mortality rate of ginseng (0.2 ~ 1 cm or less) according to the feed composition of salted sea cucumber Gossam Control 1 Control group 2 Experimental group first later first later first later Weight (g) 0.9 ± 0.3 2.3 ± 0.6 0.9 ± 0.3 3.4 ± 0.5 0.9 ± 0.3 6.1 ± 0.4 Mortality (%) 0 15 ± 0.2 0 9 ± 0.7 0 5 ± 0.3

Body weights and mortality rates of dietary (1 ~ 5cm) The Control 1 Control group 2 Experimental group first later first later first later Weight (g) 4.5 ± 1.2 6.2 ± 0.4 4.5 ± 1.2 8.3 ± 0.6 4.5 ± 1.2 11.7 ± 0.4 Mortality (%) 0 13 ± 0.4 0 8 ± 0.5 0 2 ± 0.7

As a result, as shown in Table 2 and Table 3, significant difference (P <0.1) was observed in the growth of the sea cucumber or the decrease of the mortality in the control group 2 and the experimental group as compared with the control group 1, The growth rate was slow compared with the group. In particular, it was confirmed that in the experimental group fed with the sea cucumber diets of the present invention, the weight of sea cucumber was increased and the mortality was significantly reduced as compared with the control groups 1 and 2.

Meanwhile, as shown in FIG. 3, the experimental group fed with the sea cucumber diets of the present invention was observed, and it was found that the metabolites produced in the metabolic process were also thinly and constantly discharged, and the continuous metabolism appeared uniformly.

< Example  2>

A slut  Analysis of body weight and mortality according to blending ratio of powder

The compound feed composition of the present invention prepared by blending Suaam powder, zeolite, seaweed, gourd powder and Bacillus bacteria through Example 1 can effectively increase growth of ginseng and ginseng and reduce mortality Based on the above results, the present inventors investigated the body length and mortality of ginseng and ginseng depending on the addition ratio of the gourd powder as a protein source in order to analyze the composition ratio of the optimum compound to maximize the effect .

The experiment was divided into two groups: ginseng (0.2 ~ 1 cm) and ginseng (1 ~ 5cm). 100 ginseng (0.9 g of ginseng and 4.5 ± 1.2 g of ginseng) were used. Feedstuffs were divided into three experimental groups (see Table 4 below) according to the mixing ratio of the dough powder, and the growth rate and mortality were respectively measured and the total experimental period was 3 months. The temperature was set so that the constant temperature of the sea cucumber was kept constant at 16 ° C. Statistical significance was tested by ANOVA-test.

Mixed diets for sea cucumber with different mixture ratio Feed composition Experiment 1 Experiment 2 Experiment group 3 Suam jade powder 48 wt% 44 wt% 40 wt% Zeolite 20 wt% 20 wt% 20 wt% Seaweed 5 wt% 5 wt% 5 wt% Kelp 5 wt% 5 wt% 5 wt% A witch 5 wt% 5 wt% 5 wt% Dough powder 4 wt% 8 wt% 12 wt% Bacillus 2 wt% 2 wt% 2 wt% Nutrients 8 wt% 8 wt% 8 wt% vitamin 3 wt% 3 wt% 3 wt%

Body weight and mortality rate of ginseng (0.2 ~ 1 cm or less) according to the dietary supplementation of sea cucumber with different composition ratio Gossam Experiment 1 Experiment 2 Experiment group 3 first later first later first later Weight (g) 0.9 ± 0.3 3.5 ± 0.3 0.9 ± 0.3 6.0 ± 0.8 0.9 ± 0.3 4.5 ± 0.2 Mortality (%) 0 10 ± 0.2 0 5 ± 0.3 0 9 ± 0.5

Body weight and mortality rate of ginseng (1 ~ 5cm) according to dietary supplementation of sea cucumber with different mixture ratio The Experiment 1 Experiment 2 Experiment group 3 first later first later first later Weight (g) 4.5 ± 1.2 8.0 ± 0.5 4.5 ± 1.2 11 ± 0.5 4.5 ± 1.2 9.0 ± 0.2 Mortality (%) 0 8 ± 0.4 0 2 ± 0.5 0 5 ± 0.7

As a result, as shown in Table 5 and Table 6, it was confirmed that the growth and survival rate of the sea cucumber were significantly higher in the experimental group 2 containing 8% by weight of the gully powder, and thus the growth of ginseng and ginseng was effectively increased, The experimental group 2 is the most suitable for the compounded dietary composition for sea cucumbers having the optimum compounding ratio to reduce the amount of the sea cucumber. In view of the integrated results of Example 1, It was considered to be most effective for growth of sea cucumber.

In conclusion, it is interpreted that mulberry and jade powder help to reduce stress along with feeding and growth of sea cucumber and induce adaptation to the environment. In addition, it is considered that the animal high protein of the duckweed powder was absorbed and influenced the growth. In addition, it is considered that the bacillus bacteria used directly induce the degradation and absorption of the protein and the growth, directly. In addition, considering the industrial aspect, the cheaper price of jade powder is cheaper than that of midge, so it can be helpful in inducing economic efficiency in industrial blending efficiency. On the other hand, it was thought that it was difficult to industrialize because the unit price of sea cucumber feed was high, and there was no proper mixing ratio or industrial structure. However, this experiment has provided the industrial basis for reducing the import substitution effect and the unit price of the whole raw material, It is thought that this will be a basis for enhancing economic efficiency through reduction of mortality rate.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (8)

A composition for feed of sea cucumber comprising, as an active ingredient, a powder of jade, a mineral, a seaweed, a gourd powder and a bacillus bacterium. The method according to claim 1,
Wherein the mineral is zeolite. The method according to claim 1,
Wherein the seaweed is at least one selected from the group consisting of seaweed, kelp, and sea weed. 4. The method according to any one of claims 1 to 3,
Characterized in that said composition comprises 30 to 60 parts by weight of a jade powder, 10 to 30 parts by weight of a mineral, 5 to 25 parts by weight of seaweed, 5 to 10 parts by weight of a gruel powder and 1 to 5 parts by weight of a bacillus bacterium. Composition for feed. (a) grinding each of jade powders, minerals, seaweeds and gravel powder to a size of 500 to 100 mesh; And
(b) adding a Bacillus bacterium to the prepared jade powders, minerals, algae and gravel powder and mixing them.
6. The method of claim 5,
Wherein the mineral is zeolite. 6. The method of claim 5,
Wherein the seaweed is at least one selected from the group consisting of seaweed, kelp, and insect. 6. The method of claim 5,
The composition ratio of the respective components to be mixed in the step (b) is 30 to 60 parts by weight of the oak powder, 10 to 30 parts by weight of the mineral, 5 to 25 parts by weight of the seaweed, 5 to 10 parts by weight of the gourd powder, 1 to 5 parts by weight of the composition.

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