CN101433595A - Desertliving cistanche alcohol extract as well as preparation method and use thereof - Google Patents
Desertliving cistanche alcohol extract as well as preparation method and use thereof Download PDFInfo
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- CN101433595A CN101433595A CNA200810231291XA CN200810231291A CN101433595A CN 101433595 A CN101433595 A CN 101433595A CN A200810231291X A CNA200810231291X A CN A200810231291XA CN 200810231291 A CN200810231291 A CN 200810231291A CN 101433595 A CN101433595 A CN 101433595A
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- alcohol extract
- cistanche deserticola
- desertliving cistanche
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Abstract
The invention discloses an ethanol extract of cistanche deserticola, a preparation method and application thereof. The extract is prepared by the method comprising the following steps: crushing a dry product of cistanche deserticola, cold soaking the dry product of the cistanche deserticola for two to four times by edible ethanol according to the volume ratio of the edible ethanol to the cistanche deserticola being between 2 to 1 and 4 to 1, filtering the mixture, mixing the filtrate, and reclaiming solvent at reduced pressure to obtain the ethanol extract of the cistanche deserticola. By using the ethanol extract of the cistanche deserticola to lavage, and using carbon tetrachloride to induce liver damage, ALT and AST values in blood serum of a mouse, and SOD and MDA values in liver tissue homogenate are detected; and experiments show that the ethanol extract of the cistanche deserticola can effectively reduce the ALT value, the AST value and the MDA value of the mouse with the liver damaged by the carbon tetrachloride, and can remarkably raise the SOD activity value of liver cells of the mouse. Experiments prove that the ethanol extract of the cistanche deserticola has better functions for protecting the liver and controlling the liver damage.
Description
Technical field
The present invention relates to a kind ofly, relate in particular to a kind of desertliving cistanche alcohol extract, also relate to the preparation method and the application of this desertliving cistanche alcohol extract simultaneously with Herba Cistanches extract.
Background technology
Liver is the center organ of general metabolism and nutrition, and the synthetic and decomposition of carbohydrate, protein (aminoacid), fat is mainly finished by liver.Simultaneously, there is multivitamin to synthesize, activate and store by liver.In addition, liver also has the biotransformation function, and most of endogenous and exogenous metabolism dead end product can excrete with the form of safety after need transforming by liver.And hepatic disease is one of the most common disease that influences human health.The hepatic injury that various harmful factors cause mainly contains types such as viral hepatitis, alcoholic hepatitis and drug induced hepatitis, and its clinical manifestation is hepatic necrosis, fatty liver, cholestasis, hepatic fibrosis, liver cirrhosis and hepatocarcinoma etc.At present, the treatment to hepatic injury is still global severe problem.
Herba Cistanches has another name called Herba Cistanches, rues greatly, golden Radix Crotalariae szemoensis and Herba Stellariae Saxatilis, is the dry meat stem of the band scale of orobanchaceae plant cistanche (Cistanche deset-icola Y.C.Ma).Beginning is stated from Shennong's Herbal, classifies as top gradely, and how on the books " Bencao Jingshu ", " book on Chinese herbal medicine converge with speech " and " book on Chinese herbal medicine is looked for the truth " etc. are also.Li Shizhen (1518-1593 A.D.) is said: " this thing is mended and not high, so have calmly." Herba Cistanches sweet in the mouth, salty, warm in nature, have the function of kidney-replenishing, benefiting essence-blood, loosening bowel to relieve constipation, be used for sexual impotence, infertile, soreness of the waist and knees, muscles and bones is unable and the dryness of the intestine constipation.In the use history in existing more than 1000 year of China, the laudatory title of " desert Radix Ginseng " is arranged.Herba Cistanches has 22 kinds approximately in the whole world, China has 6 kinds, comprises Desert Herba Cistanches, Saline Cistanche Herb, spends salt Herba Cistanches, Herba Cistanches sinensis and Cistanche Tubulosa in vain.Studies show that Herba Cistanches mainly contains multiple compositions such as phenethyl alcohol glycosides, iridoids, lignanoids, polysaccharide, alkaloid.Modern pharmacology shows that Herba Cistanches removes as traditional kidney invigorating and YANG supporting Chinese medicine, also has multiple pharmacologically actives such as enhancing immunity, defying age, hypermnesis and antioxidation.But in application aspect the treatment hepatopathy any report is not arranged as yet for Herba Cistanches.
Summary of the invention
The object of the present invention is to provide a kind of ethanol extraction of Chinese medicine Herba Cistanches.
Simultaneously, the present invention also aims to provide a kind of preparation method of desertliving cistanche alcohol extract.
The present invention also aims to provide the application of a kind of desertliving cistanche alcohol extract aspect preparation treatment hepatic disease; And in the medicine of preparation control hepatic injury, use.
To achieve these goals, technical scheme of the present invention has adopted a kind of desertliving cistanche alcohol extract, this extract is got by following method preparation, with the Herba Cistanches dry product, pulverize, with edible ethanol is edible ethanol by volume: Herba Cistanches=(2-4): 1 merceration 2-4 times, after filtration, merging filtrate and decompression and solvent recovery promptly get desertliving cistanche alcohol extract.
Described merceration is 3 times, wherein for the first time 6-8, and back twice 2-4 days.
Technical scheme of the present invention has also adopted a kind of preparation method of desertliving cistanche alcohol extract, may further comprise the steps: the Herba Cistanches dry product, pulverize, with edible ethanol is edible ethanol by volume: Herba Cistanches=(2-4): 1 merceration 2-4 times, after filtration, merging filtrate and decompression and solvent recovery promptly get desertliving cistanche alcohol extract.
Described merceration is 3 times, wherein for the first time 6-8, and back twice 2-4 days.
Technical scheme of the present invention has adopted the application of a kind of desertliving cistanche alcohol extract aspect preparation treatment hepatic disease.
Also adopted a kind of desertliving cistanche alcohol extract in the medicine of preparation control hepatic injury, to use simultaneously.
Ethanol extraction with Herba Cistanches is irritated stomach, with the tetrachloro-methane induction hepatic injury, detect ALT and AST value in the mice serum, and SOD in the liver tissue homogenate and MDA value, experiment shows: desertliving cistanche alcohol extract can effectively reduce the ALT value of carbon tetrachloride hepatic injury mice, AST value and MDA value, and the SOD energy value of the mouse liver cell that can raise significantly.The proof desertliving cistanche alcohol extract has the effect of good the liver protecting and control hepatic injury.
By adopting method of the present invention to handle the desertliving cistanche alcohol extract of gained, can make various preparations, comprising: suppository, tablet, pill, granule, membrane, microcapsule, drop pill, aerosol, medicated wine, syrup, oral liquid and injection.Herba Cistanches extract provided by the present invention is evident in efficacy for treatment hepatic disease, improvement and recovery liver function aspect, and can be used as and be used for liver-protective health product and use.
The specific embodiment
Embodiment 1
Present embodiment is a Herba Cistanches extract---the extracting method at ethanol position, specifically may further comprise the steps: get the Herba Cistanches dry product, pulverize, with edible ethanol is 3:1 merceration 3 times by volume, 7 days for the first time, the back is 3 days twice, filters, and merging filtrate and decompression and solvent recovery promptly get desertliving cistanche alcohol extract.
Embodiment 2
Present embodiment is the liver protection experiment of desertliving cistanche alcohol extract.
Method: intracorporal method-carbon tetrachloride acute liver damage model
Principle: CCl
4Be to use the earliest, hepatic fibrosis derivant the most widely, in the acute liver damage experiment, also be common drug, about CCl
4Liver toxicity mechanism, study morely, have multiple suggestion, but all consistently generally acknowledge that the chain type peroxidization of the formation of free radical and initiation is its mainly mechanism.For CCl
4In the stability study of mouse liver injury model, mainly be CCl by various dose
4Oleum Arachidis hypogaeae semen, hepatic injury due to after injection detects the superoxide dismutase SOD in glutamate pyruvate transaminase (ALT/GPT), glutamic oxaloacetic transaminase, GOT (AST/GOT) and the hepatic tissue in the mice serum in the certain hour), the content of malonaldehyde (MDA) judges the liver damage situation of mice.ALT and AST mainly are distributed in the hepatocyte of liver, if hepatic necrosis ALT and AST will raise.But these two kinds of enzyme distributions in hepatocyte are different.ALT is distributed in liver cytoplasm, and AST is distributed in liver cytoplasm and the mitochondrion.The chronic hepatitis of acute hepatitis and light disease mainly shows as the rising of ALT.Therefore, AST/ALT＜1; In the later stage of chronic hepatitis, liver cirrhosis and liver cancer patient, hepatocellular destructiveness is serious, mitochondrion also has been subjected to serious destruction, therefore, AST raises obviously, AST/ALT〉1 in addition 2.ALT and AST mainly are distributed in the hepatocyte of liver, and normal value is 0～40 iu.If hepatic necrosis, ALT and AST will raise, and are present the most frequently used liver function indexs.
Instrument: UV-2000 type ultraviolet-uisible spectrophotometer (but You Ni Shanghai Instr Ltd.), CS-H1 type blender (Beijing is won and encouraged positive scientific ﹠ technical corporation), the biochemical incubator (Shanghai one permanent Science and Technology Ltd.) of LRH-150, Multiskan MK3 microplate reader (thermoelectric Shanghai Instr Ltd.), TGL-16 high speed centrifuge (big instrument plant in Jintan City, Jiangsu), the tissue refiner of 985370-395 type (BIOSREC, Mexico), all size pipettor etc.
Reagent: bifendate drop pill (Zhejiang Prov WanBang Pharmaceutical Co., Ltd; lot number: 080210); ALT, AST, SOD, MDA detection kit (be Nanjing and build up bio-engineering research institute); DANGFEI LIGANNING JIAONANG (Meidakang Pharmaceutical Co., Ltd., Sichuan Prov.; product batch number: 080302); (packing factory of Shanghai chemical reagents corporation, lot number: 20050115), other reagent are analytical pure to Coomassie brilliant blue G250.
Method:
(20 ± 2g) are divided into 7 groups to mice at random, and 10 every group, blank group, model group are irritated stomach (ig) and give 0.5% CMC-Na solution 20mL/kg every day; The bifendate drop pill positive controls is irritated stomach 100mg/kg every day, the DANGFEI LIGANNING JIAONANG positive controls is irritated stomach 1400mg/kg every day, the desertliving cistanche alcohol extract height, in, low dose group is irritated stomach 1000mg/kg respectively, 500mg/kg, 250mg/kg administration 8d, behind last administration 2h, except that the blank group all according to 0.1mL/10g lumbar injection 0.5% carbon tetrachloride oil solution, after water 16h is can't help in fasting, plucking eyeball gets blood and isolates serum, detect ALT and AST, isolating liver weighs, and the liver of the about 300mg of weighing and about 30mg becomes the liver homogenate liquid of 10% (surveying MDA) and 1% (surveying SOD) with 0-3 ℃ of normal saline homogenate, measure MDA and SOD, Coomassie brilliant blue removes albumen.
The mensuration of ALT, AST, SOD and MDA is all carried out according to the requirement of test kit, and experimental data x ± s represents that experiment statistics adopts the spss10.0 statistical software to carry out one factor analysis of variance.
The result:
(1) desertliving cistanche alcohol extract the results are shown in Table 1 to the influence of carbon tetrachloride acute liver damage mice ALT and AST, shows in the table, and the ALT and the AST energy value of desertliving cistanche alcohol extract dosage group all are starkly lower than model group, and data difference has the significance meaning.And the degree with its reduction of reduction of dosage reduces, and illustrates to have dose dependent.
(2) desertliving cistanche alcohol extract to the influence of carbon tetrachloride acute liver damage anti-oxidative ability of mice as can be known by table 1 data, the SOD level of model group is starkly lower than the blank group, and the SOD level of Herba Cistanches dosage group is apparently higher than model group; Even being higher than the blank group, the MDA level is starkly lower than model group.Show that desertliving cistanche alcohol extract may come the liver protecting tissue by oxidative pathway (increased SOD, reduction MDA).
Table 1 desertliving cistanche alcohol extract is to the protective effect of carbon tetrachloride acute liver damage (x ± s)
Each group (except that the sample blank group) and model are compared:
*P＜0.05,
*P＜0.01,
* *P＜0.001; Model group and blank group compare: #P＜0.05, ##P＜0.01, ###P＜0.001
Experimental result shows, the carbon tetrachloride model group compares with blank group matched group, the MDA value all obviously raises in serum alt, AST value and the hepatocyte, the SOD value reduces, the modeling success is described, each dosage group of desertliving cistanche alcohol extract all has ALT, AST of reduction carbon tetrachloride acute liver damage mice and the value of MDA, promotes the SOD activity level of mice simultaneously.As seen desertliving cistanche alcohol extract has good hepatoprotective effect.
Embodiment 3
The preparation of desertliving cistanche alcohol extract tablet:
Preparation method: get above-mentioned Herba Cistanches extract 100g, the ethanol of adding 80% is made the wetting agent mixing and is made soft material behind adding starch 250g, the arabic gum 15g, under 60 ℃ temperature, be dried to water content below 3%, then the material of oven dry was pulverized 14 mesh sieves, after adding magnesium stearate 5g and Pulvis Talci 20g mixing at last, cross 12 mesh sieve granulate, tabletting, quality inspection, coating are made 1000.
It should be noted last that: above embodiment is only in order to explanation, and unrestricted technical scheme of the present invention, although the present invention is had been described in detail with reference to the foregoing description, those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (6)
1, a kind of desertliving cistanche alcohol extract, it is characterized in that: this extract is got by following method preparation, with the Herba Cistanches dry product, pulverize, with edible ethanol is edible ethanol by volume: Herba Cistanches=(2-4): 1 merceration 2-4 times, after filtration, merging filtrate and decompression and solvent recovery promptly get desertliving cistanche alcohol extract.
2, desertliving cistanche alcohol extract according to claim 1 is characterized in that: described merceration is 3 times, is 6-8 days for the first time wherein, and the back is 2-4 days twice.
3, a kind of preparation method of desertliving cistanche alcohol extract as claimed in claim 1, it is characterized in that: may further comprise the steps: the Herba Cistanches dry product, pulverize, with edible ethanol is edible ethanol by volume: Herba Cistanches=(2-4): 1 merceration 2-4 times, after filtration, merging filtrate and decompression and solvent recovery promptly get desertliving cistanche alcohol extract.
4, desertliving cistanche alcohol extract according to claim 3 is characterized in that: described merceration is 3 times, wherein 6-8 days for the first time, and back twice 2-4 days.
5, the application of a kind of desertliving cistanche alcohol extract aspect preparation treatment hepatic disease.
6, the application of a kind of desertliving cistanche alcohol extract in the medicine of preparation control hepatic injury.
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| CNA200810231291XA CN101433595A (en) | 2008-12-10 | 2008-12-10 | Desertliving cistanche alcohol extract as well as preparation method and use thereof |
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| CNA200810231291XA CN101433595A (en) | 2008-12-10 | 2008-12-10 | Desertliving cistanche alcohol extract as well as preparation method and use thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105687743A (en) * | 2016-03-17 | 2016-06-22 | 南京多宝生物科技有限公司 | Herba cistanches health care product |
| CN105832822A (en) * | 2016-05-17 | 2016-08-10 | 乌鲁木齐西部康维生物科技有限公司 | Herba cistanches extracting solution and preparing method thereof |
| CN106109592A (en) * | 2016-07-21 | 2016-11-16 | 李时珍医药集团有限公司 | A kind of preparation method of Flos Lonicerae cold granules |
| CN114588201A (en) * | 2022-03-30 | 2022-06-07 | 北京中科健宇生物科技有限公司 | Preparation method of cistanche deserticola extract and alcohol effect relieving and liver protecting product containing cistanche deserticola extract |
| CN115572647A (en) * | 2021-08-02 | 2023-01-06 | 向四海 | Cistanche deserticola wine and preparation method thereof |
-
2008
- 2008-12-10 CN CNA200810231291XA patent/CN101433595A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105687743A (en) * | 2016-03-17 | 2016-06-22 | 南京多宝生物科技有限公司 | Herba cistanches health care product |
| CN105832822A (en) * | 2016-05-17 | 2016-08-10 | 乌鲁木齐西部康维生物科技有限公司 | Herba cistanches extracting solution and preparing method thereof |
| CN106109592A (en) * | 2016-07-21 | 2016-11-16 | 李时珍医药集团有限公司 | A kind of preparation method of Flos Lonicerae cold granules |
| CN115572647A (en) * | 2021-08-02 | 2023-01-06 | 向四海 | Cistanche deserticola wine and preparation method thereof |
| CN114588201A (en) * | 2022-03-30 | 2022-06-07 | 北京中科健宇生物科技有限公司 | Preparation method of cistanche deserticola extract and alcohol effect relieving and liver protecting product containing cistanche deserticola extract |
| CN114588201B (en) * | 2022-03-30 | 2024-01-02 | 北京中科健宇生物科技有限公司 | Preparation method of cistanche extract and anti-alcohol liver-protecting product containing cistanche extract |
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Open date: 20090520 |