CN101400796B - 在真细菌宿主细胞内表达正交翻译组分的系统 - Google Patents

在真细菌宿主细胞内表达正交翻译组分的系统 Download PDF

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CN101400796B
CN101400796B CN2007800084039A CN200780008403A CN101400796B CN 101400796 B CN101400796 B CN 101400796B CN 2007800084039 A CN2007800084039 A CN 2007800084039A CN 200780008403 A CN200780008403 A CN 200780008403A CN 101400796 B CN101400796 B CN 101400796B
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trna
nucleotide sequence
unnatural amino
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CN101400796A (zh
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柳荣夏
P·G·舒尔茨
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Scripps Research Institute
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2007800084039A 2006-03-09 2007-03-07 在真细菌宿主细胞内表达正交翻译组分的系统 Expired - Fee Related CN101400796B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US78097306P 2006-03-09 2006-03-09
US60/780,973 2006-03-09
US78349706P 2006-03-17 2006-03-17
US60/783,497 2006-03-17
US85533606P 2006-10-29 2006-10-29
US60/855,336 2006-10-29
PCT/US2007/005914 WO2007103490A2 (en) 2006-03-09 2007-03-07 System for the expression of orthogonal translation components in eubacterial host cells

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CN101400796A CN101400796A (zh) 2009-04-01
CN101400796B true CN101400796B (zh) 2013-03-27

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CN2007800084039A Expired - Fee Related CN101400796B (zh) 2006-03-09 2007-03-07 在真细菌宿主细胞内表达正交翻译组分的系统

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Country Link
US (3) US7960141B2 (https=)
EP (1) EP1991680B1 (https=)
JP (1) JP5589186B2 (https=)
KR (1) KR20080106430A (https=)
CN (1) CN101400796B (https=)
AU (1) AU2007223830A1 (https=)
BR (1) BRPI0709935A2 (https=)
CA (1) CA2638763A1 (https=)
ES (1) ES2432391T3 (https=)
IL (1) IL193394A0 (https=)
MX (1) MX2008011470A (https=)
PL (1) PL1991680T3 (https=)
PT (1) PT1991680E (https=)
WO (1) WO2007103490A2 (https=)

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MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
WO2005019415A2 (en) 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
US20080171317A1 (en) * 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
EP1807515A4 (en) 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US7960141B2 (en) * 2006-03-09 2011-06-14 The Scripps Research Institute Systems for the expression of orthogonal translation components in eubacterial host cells
KR20080113226A (ko) 2006-03-16 2008-12-29 더 스크립스 리서치 인스티튜트 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현
US7807411B2 (en) 2006-05-23 2010-10-05 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
EP2052083B1 (en) 2006-09-21 2014-01-08 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
JP2010506591A (ja) 2006-10-18 2010-03-04 ザ スクリップス リサーチ インスティテュート 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
AU2008335778B2 (en) 2007-12-11 2014-04-10 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
CN101970005A (zh) * 2008-02-08 2011-02-09 斯克利普斯研究院 用遗传编码的非天然氨基酸打破免疫耐受
PE20110480A1 (es) 2008-09-26 2011-07-01 Ambrx Inc Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales
CN102307905B (zh) 2008-12-10 2015-11-25 斯克利普斯研究院 利用化学反应性非天然氨基酸产生载体-肽偶联物
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
MA34521B1 (fr) * 2010-08-17 2013-09-02 Ambrx Inc Polypeptides de relaxine modifiés et leurs utilisations
JP6301316B2 (ja) 2012-05-23 2018-03-28 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 内胚葉細胞および肝実質細胞の組成物ならびにそれらの細胞を入手および使用する方法
JO3564B1 (ar) 2013-03-08 2020-07-05 Novartis Ag ببتيدات وتركيبات لعلاج ضرر المفاصل
US10005838B2 (en) 2013-07-19 2018-06-26 The Regents Of The University Of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
CN109963579A (zh) 2016-11-14 2019-07-02 诺华股份有限公司 用于治疗软骨损伤和关节炎的方法和组合物
EP3538546B1 (en) 2016-11-14 2025-01-08 Novartis AG Compositions, methods, and therapeutic uses related to fusogenic protein minion
CN110637027B (zh) 2017-02-08 2024-08-30 百时美施贵宝公司 包含药代动力学增强子的修饰的松弛素多肽及其用途
US20220389466A1 (en) * 2019-11-05 2022-12-08 Nitro Biosciences, Inc. Biosynthesis of para-nitro-l-phenylalanine
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
CN113322267B (zh) * 2021-03-15 2024-01-05 天津大学 系统在提高非天然氨基酸的插入效率中的应用
WO2024091824A1 (en) 2022-10-26 2024-05-02 Ada Forsyth Institute, Inc. Differentiation and reprogramming of chondrocyte

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WO2005003294A2 (en) * 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
WO2005019415A2 (en) * 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof

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WO2004094593A2 (en) * 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
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WO2005019415A2 (en) * 2003-07-07 2005-03-03 The Scripps Research Institute Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof

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EP1991680A4 (en) 2009-10-21
US8357512B2 (en) 2013-01-22
US20090181429A1 (en) 2009-07-16
EP1991680A2 (en) 2008-11-19
JP2009529328A (ja) 2009-08-20
EP1991680B1 (en) 2013-08-21
JP5589186B2 (ja) 2014-09-17
PL1991680T3 (pl) 2014-01-31
AU2007223830A1 (en) 2007-09-13
US20120252067A1 (en) 2012-10-04
PT1991680E (pt) 2013-10-30
WO2007103490A2 (en) 2007-09-13
CN101400796A (zh) 2009-04-01
US7960141B2 (en) 2011-06-14
ES2432391T3 (es) 2013-12-03
KR20080106430A (ko) 2008-12-05
CA2638763A1 (en) 2007-09-13
MX2008011470A (es) 2008-09-24
IL193394A0 (en) 2009-09-22
WO2007103490A3 (en) 2008-06-12
BRPI0709935A2 (pt) 2011-08-02
US8198044B2 (en) 2012-06-12
US20070281335A1 (en) 2007-12-06

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