CN101389750B - 用于直接表达肽的改进的细菌宿主细胞 - Google Patents
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Biomedical Technology (AREA)
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- General Chemical & Material Sciences (AREA)
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| US55282404P | 2004-03-12 | 2004-03-12 | |
| US60/552,824 | 2004-03-12 | ||
| US11/076,260 US8227241B2 (en) | 2004-03-12 | 2005-03-09 | Bacterial host cell for the direct expression of peptides |
| US11/076,260 | 2005-03-09 | ||
| PCT/US2005/007873 WO2005089182A2 (fr) | 2004-03-12 | 2005-03-10 | Ameliorations apportees a une cellule hote bacterienne destinee a l'expression directe de peptides |
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| CN201110107199.4A Division CN102337241B (zh) | 2004-03-12 | 2005-03-10 | 用于直接表达肽的改进的细菌宿主细胞 |
| CN2011101072554A Division CN102337286A (zh) | 2004-03-12 | 2005-03-10 | 用于直接表达肽的改进的细菌宿主细胞 |
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| CN101389750A CN101389750A (zh) | 2009-03-18 |
| CN101389750B true CN101389750B (zh) | 2012-07-11 |
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| CN2011101072554A Pending CN102337286A (zh) | 2004-03-12 | 2005-03-10 | 用于直接表达肽的改进的细菌宿主细胞 |
| CN201110107199.4A Expired - Fee Related CN102337241B (zh) | 2004-03-12 | 2005-03-10 | 用于直接表达肽的改进的细菌宿主细胞 |
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| CN2011101072554A Pending CN102337286A (zh) | 2004-03-12 | 2005-03-10 | 用于直接表达肽的改进的细菌宿主细胞 |
| CN201110107199.4A Expired - Fee Related CN102337241B (zh) | 2004-03-12 | 2005-03-10 | 用于直接表达肽的改进的细菌宿主细胞 |
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| EP (2) | EP2067854B1 (fr) |
| JP (2) | JP5591447B2 (fr) |
| CN (3) | CN101389750B (fr) |
| AU (2) | AU2005222860A1 (fr) |
| CA (2) | CA2795418C (fr) |
| DK (2) | DK2067854T3 (fr) |
| ES (2) | ES2440658T3 (fr) |
| WO (1) | WO2005089182A2 (fr) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8227241B2 (en) * | 2004-03-12 | 2012-07-24 | Unigene Laboratories, Inc. | Bacterial host cell for the direct expression of peptides |
| US7445911B2 (en) * | 2004-11-24 | 2008-11-04 | Unigene Laboratories Inc. | Enzymatic reactions in the presence of keto acids |
| EP1907561B1 (fr) | 2005-06-24 | 2013-08-28 | Unigene Laboratories, Inc. | Lignees cellulaires permettant d'exprimer une enzyme utile pour la preparation de produits amides |
| EP2061897B1 (fr) | 2006-07-27 | 2020-03-25 | Wyeth LLC | Procede de fermentation en lot alimente a haute densite cellulaire pour obtenir une proteine recombinante |
| US8377863B2 (en) | 2007-05-29 | 2013-02-19 | Unigene Laboratories Inc. | Peptide pharmaceutical for oral delivery |
| WO2013052168A2 (fr) * | 2011-05-26 | 2013-04-11 | Georgetown University | Protéine aib1-delta de détection |
| US20150353940A1 (en) * | 2013-08-05 | 2015-12-10 | Absci, Llc | Vectors for use in an inducible coexpression system |
| CA2904327C (fr) | 2013-03-05 | 2021-06-08 | Enteris Biopharma, Inc. | Produits pharmaceutiques pour une administration par voie orale |
| JP2016527911A (ja) | 2013-08-20 | 2016-09-15 | レック・ファーマシューティカルズ・ディー・ディーＬｅｋ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｄ．Ｄ． | ポリペプチドのα−アミド化および／またはＣ末端アミノ酸開裂を制御するための細胞培養用培地およびプロセス |
| CN103911390B (zh) * | 2014-04-24 | 2017-02-15 | 江南大学 | 一种通过调节OmpA表达提高大肠杆菌黄酮类物质耐受性的方法 |
| US9833411B2 (en) | 2015-01-12 | 2017-12-05 | Enteris Biopharma, Inc. | Solid oral dosage forms |
| CN109153716A (zh) | 2016-01-06 | 2019-01-04 | 安口生物公司 | 调节单克隆抗体组合物中的非岩藻糖基化物类 |
| CA3013336A1 (fr) | 2016-02-03 | 2017-08-10 | Oncobiologics, Inc. | Formulations de tampon pour ameliorer la stabilite d'anticorps |
| CN107365779A (zh) * | 2017-08-22 | 2017-11-21 | 中国科学院天津工业生物技术研究所 | 利用谷氨酸棒状杆菌生产紫色杆菌素的方法 |
| JP2022525775A (ja) | 2019-03-18 | 2022-05-19 | バイオ－ラッド エービーディー セロテック ゲーエムベーハー | ＳｐｙＴａｇ含有ペリプラズム融合タンパク質のプロテアーゼＴＳＰ及びＯＭＰＴ分解からの保護 |
| CN114875063A (zh) * | 2022-02-28 | 2022-08-09 | 华侨大学 | 一种提高植物基础抗逆的方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6103495A (en) * | 1997-04-16 | 2000-08-15 | Unigene Laboratories, Inc. | Direct expression of peptides into culture media |
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| US4595658A (en) * | 1982-09-13 | 1986-06-17 | The Rockefeller University | Method for facilitating externalization of proteins synthesized in bacteria |
| DE3586386T2 (de) | 1984-10-05 | 1993-01-14 | Genentech Inc | Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. |
| US5223407A (en) * | 1988-08-31 | 1993-06-29 | Allelix Inc. | Excretion of heterologous proteins from e. coli |
| US5264365A (en) * | 1990-11-09 | 1993-11-23 | Board Of Regents, The University Of Texas System | Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides |
| US6201925B1 (en) * | 1996-11-15 | 2001-03-13 | Futuretel, Inc. | Method and apparatus for editing video files |
| IL156111A0 (en) | 2000-12-14 | 2003-12-23 | Genentech Inc | Bacterial host strains |
| EP1279739A1 (fr) * | 2001-07-26 | 2003-01-29 | Vrije Universiteit Brussel | Vecteurs recombinants derivés des virus adéno-associés exprimant TAM67 pour la thérapie génique |
| US8227241B2 (en) * | 2004-03-12 | 2012-07-24 | Unigene Laboratories, Inc. | Bacterial host cell for the direct expression of peptides |
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- 2005-03-10 WO PCT/US2005/007873 patent/WO2005089182A2/fr not_active Application Discontinuation
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- 2005-03-10 CN CN200580007801XA patent/CN101389750B/zh not_active Expired - Fee Related
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6103495A (en) * | 1997-04-16 | 2000-08-15 | Unigene Laboratories, Inc. | Direct expression of peptides into culture media |
Non-Patent Citations (1)
| Title |
|---|
| BANEYX F.等.Construction and characterization of Escherichia coli strains deficient in multiple secreted protease：protease III degrades high- molecular-weight substrates in vivo.《JOURNAL OF BACTERIOLOGY》.American Society for Microbiology,1991,第173卷(第8期),摘要. * |
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| US6210925B1 (en) | Direct expression of peptides into culture media | |
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| AU2012200936A1 (en) | Improved bacterial host cell for the direct expression of peptides | |
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