CN101380341B - Preparation method of compound propolis preparation - Google Patents
Preparation method of compound propolis preparation Download PDFInfo
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- CN101380341B CN101380341B CN2007100300307A CN200710030030A CN101380341B CN 101380341 B CN101380341 B CN 101380341B CN 2007100300307 A CN2007100300307 A CN 2007100300307A CN 200710030030 A CN200710030030 A CN 200710030030A CN 101380341 B CN101380341 B CN 101380341B
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- propolis
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- octacosanol
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Abstract
The invention discloses a compound propolis preparation and a preparation method thereof, and relates to the technical field of biochemistry. The compound propolis preparation is a capsule which is prepared by raw materials of propolis, sporoderm-broken ganoderma lucidum spore powder and octacosanol with the weight percentage of 50-70 to 20-40 to 1-10. The propolis is prepared by the following steps: extracting raw propolis by a complex solvent of 70-75% of alcohol and 20% of 1, 2-propanediol, and furthermore extracting by the supercritical CO2 and obtaining the high-quality propolis taken as the raw material, taking the cane wax as the raw material of the octacosanol, and using the supercritical CO2 extraction method. The compound propolis preparation provided by the invention has great health care function.
Description
Technical field
The present invention relates to technological field of biochemistry, specifically a kind of novel propolis compound preparation.
Background technology
To be Apis germinate or resin that bark is gathered from plant (as some long-lived plants: pinaster, willow, birch etc.) is newborn propolis, adds pollen and the secretions of last frontal gland and the opaque colloidal solids of armaticity that cured matter processes through Apis.In blob of viscose hardening below 20 ℃, the blob of viscose deliquescing has stickiness more than 20 ℃.The elite of propolis collection animal, plant secretion thing has complicated and magical biological activity.
The full son of Ganoderma belongs to Basidiomycetes, Polyporaceae.Ganoderma is traditional strengthening by means of tonics medicine, and property is flat, slightly sweet flavor, little hardship.The seed that the full son of Ganoderma is the Ganoderma breeding has concentrated Ganoderma heredity active.
Octacosanol early extracted from wheat germ before this and obtains and find that it has material to human body and the unique physiologically active of animal.Octacosanol extensively is present in the lipid of many plants, cured matter, the frumentum oils and fats, and the raw material with actual exploitation meaning comprises Cera Flava, and the cured and Testa oryzae of sugarcane is cured etc.We use supercritical CO
2The octacosanol that method is extracted from sugarcane is cured has the excitometabolic of adding, the effect of conditioning system function.
Summary of the invention
It is primary raw material that purpose of the present invention just provides a kind of high-quality propolis, adds full sub-powder of wall-breaking lucidum and supercritical CO
2The octacosanol of extraction and a kind of compound bee-glue preparation of making, the health care of propolis can be worked in coordination with and strengthen to this novel formulation.
Technical scheme of the present invention is by propolis: Ganoderma spore powder with cellular wall broken: the percentage by weight of octacosanol is 05～70: 20～40: the compound bee-glue capsule that 1～10 raw material is made.
The preparation method of compound bee-glue preparation is a preparation technique routinely, and propolis, Ganoderma spore powder with cellular wall broken, octacosanol are made capsule by the prescription mixed.The preparation of its said propolis prepares as follows:
A, propolis at first add the double solvents extraction hair glue of the 1.2-propylene glycol of ethanol weight 20% with 70～75% ethanol,
The extracting solution of b, above-mentioned a is that propolis liquid drops into supercritical CO
2Extraction kettle is further used supercritical CO
2Extraction is to obtain high-quality propolis, supercritical CO
2Extraction conditions is: extracting pressure 20～40Mpa, and 30～70 ℃ of extraction temperature, 2～6 hours extraction time, the first order is resolved pressure and is lower than 20Mpa, 20～50 ℃ of resolution temperatures, pressure is resolved less than 15Mpa in the second level, 20～50 ℃ of resolution temperatures;
The preparation method of octacosanol: cured with sugarcane is raw material, uses supercritical CO
2Extracting process prepares octacosanol, its extracting pressure 15～50Mpa, and 30 ℃～60 ℃ of extraction temperature, the extraction time, adopting entrainer was water or ethanol less than 4 hours.
Through our experimental results show that: 70～75% concentration ethanol can reasonablely obtain the fat-soluble and water solublity two class materials in the propolis, contrast experiment's analysis result, and its general flavone content exceeds 1～2 percentage point than other concentration ethanol extractions.This shows concentration of alcohol too high or too low all have biased.The concentration of alcohol that propolis is carried out compound extracting that provides among the patent CN1389218A is 80% and 90% two kind.The concentration of alcohol that propolis is soaked into processing that provides among the CN1258511A is 95% and dehydrated alcohol, and is favourable not as good as 70～75% ethanol to the extraction of water solublity thing in the propolis like this.We add dissolving, stirring, the filtration that the 20%1.2-propylene glycol helps propolis more in 70～75% ethanol, can shorten the flow process time.The solid-to-liquid ratio of solvent extraction is 1: (7～10), extraction time is 2～3 days.
The present invention is achieved in that the compound bee-glue preparation is by propolis: Ganoderma spore powder with cellular wall broken: the percentage by weight of octacosanol is 50～70: 20～40: 1～10 raw material, the capsule made from conventional method.
The preparation method of compound bee-glue preparation, wherein:
The preparation method of A, propolis:
A, thick glue (being hair glue again) contain a lot of impurity, so need through remove impurity ,-18 ℃ of left and right sides freeze overnight, pulverize then, sieve, and soak 2～3 days with 70～75% edible ethanols (adding the 20%1.2-propylene glycol), stir, and filter to get filtrate;
The extracting solution of b, above-mentioned a gained is further used supercritical CO
2Extraction is to obtain high-quality propolis, supercritical CO
2Extraction conditions is: extracting pressure 20～40Mpa, and 30～70 ℃ of extraction temperature, 2～6 hours extraction time, the first order is resolved pressure and is lower than 20Mpa, 20～50 ℃ of resolution temperatures, the second level is resolved pressure and is lower than 15Mpa, 20～50 ℃ of resolution temperatures.
The preparation method of B, octacosanol be cured with sugarcane be raw material, supercritical CO
2Extraction, its extracting pressure 15～50Mpa, 30 ℃～60 ℃ of extraction temperature, the extraction time, adopting entrainer was water or ethanol less than 4 hours.
C, the full sub-powder of wall-breaking lucidum are commercially available health product.Sporoderm-broken rate requires greater than 95%, and water content is not higher than 9%.
The specific embodiment
Supercritical CO
2The propolis of extraction is faint yellow gluey body, through freezing, pulverizes, and sieves and the full sub-powder of wall-breaking lucidum, supercritical CO
2The octacosanol (purity 50%) of extraction is in following ratio compatibility: 50～70% to 20～40% to 1～10% (W/W).Compound preparation can be hard capsule or other dosage form.
The preparation of embodiment 1 propolis
Embodiment 1-A: ethanol extraction
Embodiment 1-B: supercritical CO
2Extraction
The preparation of embodiment 2 propolis
Embodiment 2-A: ethanol extraction
Embodiment 2-B: supercritical CO
2Extraction
Embodiment 3
Supercritical CO
2The propolis of extraction is xanchromatic semisolid, through-18 ℃ of freeze overnight, and the pulverizing that continues, (40～60 order) sieves, then, in following ratio (W/W) propolis: Ganoderma spore powder: octacosanol=in blender mix at 65: 30: 5, be processed into hard capsule on the last filling machine.
Claims (1)
1. the preparation method of a compound bee-glue preparation, it is characterized in that it being by propolis: Ganoderma spore powder with cellular wall broken: the percentage by weight of octacosanol is 50～70%: 20～40%: 1～10% raw material mixes makes capsule according to a conventional method,
Said propolis prepares as follows:
A, get thick glue-18 ℃ of freeze overnight, pulverize then, sieve, add 1 of ethanol weight 20% with 70～75% edible ethanols, the double solvents of 2-propylene glycol soaked 2～3 days, and stirring filters to get filtrate;
The filtrate of b, above-mentioned a is further used supercritical CO
2Extraction is to obtain high-quality propolis, supercritical CO
2Extraction conditions is: extracting pressure 20～40Mpa, and 30～70 ℃ of extraction temperature, 2～6 hours extraction time, the first order is resolved pressure and is lower than 20Mpa, 20～50 ℃ of resolution temperatures, pressure is resolved less than 15Mpa in the second level, 20～50 ℃ of resolution temperatures;
The preparation method of said octacosanol: cured with sugarcane is raw material, uses supercritical CO
2Extracting process prepares octacosanol, its extracting pressure 15～50Mpa, and 30 ℃～60 ℃ of extraction temperature, the extraction time, adopting entrainer was water or ethanol less than 4 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100300307A CN101380341B (en) | 2007-09-03 | 2007-09-03 | Preparation method of compound propolis preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100300307A CN101380341B (en) | 2007-09-03 | 2007-09-03 | Preparation method of compound propolis preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101380341A CN101380341A (en) | 2009-03-11 |
| CN101380341B true CN101380341B (en) | 2011-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2007100300307A Expired - Fee Related CN101380341B (en) | 2007-09-03 | 2007-09-03 | Preparation method of compound propolis preparation |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104351761B (en) * | 2014-10-17 | 2016-08-31 | 无锡许仙草灵芝农民专业合作社 | A kind of superhigh pressure water jet Ganoderma spore powder with cellular wall broken and oral liquid process unit |
| CN104585732B (en) * | 2014-12-30 | 2016-05-18 | 广州白云山汉方现代药业有限公司 | A kind of Reishi sporule health food and preparation method thereof is applied with alleviating physical fatigue |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583161A (en) * | 2004-05-26 | 2005-02-23 | 内蒙古草原兴发股份有限公司 | Health care product of bee adhesive |
| CN101011464A (en) * | 2007-02-02 | 2007-08-08 | 扬州三邦天然保健品有限公司 | Composite propolis soft capsule |
-
2007
- 2007-09-03 CN CN2007100300307A patent/CN101380341B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583161A (en) * | 2004-05-26 | 2005-02-23 | 内蒙古草原兴发股份有限公司 | Health care product of bee adhesive |
| CN101011464A (en) * | 2007-02-02 | 2007-08-08 | 扬州三邦天然保健品有限公司 | Composite propolis soft capsule |
Non-Patent Citations (1)
| Title |
|---|
| 游鹏程.从甘蔗渣中超临界CO2萃取二十八烷醇的研究.《广东化工》.2005,(第10期),第35-37页. * |
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| CN101380341A (en) | 2009-03-11 |
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