JP2002306092A - Propolis product having antioxidant activity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a propolis product having a wide range of biological activity of the propolis and an antioxidant activity effective for prevention and improvement of aging change. SOLUTION: This propolis product having antioxidant activity comprises a mixture of extracts of the propolis obtained by extracting a clump of the raw propolis with two or more kinds of aqueous solutions of ethanol having different concentration of water, preferably the propolis extract is obtained by extracting the raw propolis with an aqueous ethanol solution including 5-10 vol.% of water and the extract obtained by extracting with an aqueous ethanol solution including 45-55 vol.% of water and then mixing both in a volumetric mixing ratio of 4-6:6-4. The propolis product having antioxidant activity preferably uses the clump of the raw propolis originating from Brazilian growth eucalyptus/rosemary.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a propolis product, and more particularly, to a propolis product having a wide range of physiological activities and excellent antioxidant activity.

[0002]

2. Description of the Related Art Propolis is a resinous substance derived from plants collected by bees or saliva of bees, which accumulates in beehives, and contains various components such as flavonoids, terpenoids, amino acids, minerals, and vitamins. It is widely used as a health food that can be expected to have a wide range of physiological activities such as anticancer, antiallergic, antibacterial and antiviral activities, and skin diseases. In particular, in the age of a super-aging society, the prevention and improvement of lifestyle-related diseases such as cancer, arteriosclerosis, diabetes, cataract, and immune dysfunction, which are expected to increase more and more, are important in national medical care. It is a problem, and it has been found that these lifestyle-related diseases are caused by the damage of living cells by active oxygen with aging, and they have antioxidant activity to prevent and improve age-related changes. Food intake is encouraged.

[0003] By the way, since propolis original mass contains many contaminants other than biologically active components, it is common to extract propolis extract from propolis original mass and take it in solid or liquid form. It is. Conventionally, methods for extracting a propolis extract from an original propolis mass include a water extraction method, an alcohol extraction method, and an alcohol aqueous solution extraction method. According to the water extraction method, a large amount of the hydrophilic component can be extracted, but only a small amount of the lipophilic component can be extracted.According to the alcohol extraction method, a large amount of the lipophilic component can be extracted, but a small amount of the hydrophilic component can be extracted. Stay in. According to the aqueous alcohol solution extraction method of extracting with a mixed solution of alcohol and water, both the hydrophilic component and the lipophilic component can be extracted.

[0004] On the other hand, there have been various reports on the physiological activity of propolis extracts obtained by various extraction methods. For example, there are reports that propolis extract obtained by water extraction method has anti-cancer properties and that propolis extract obtained by alcohol extraction has anti-allergic properties. Are expected to have a physiological activity, and the propolis extract by an aqueous alcohol solution extraction method capable of extracting both is expected to have a wide range of physiological activities possessed by propolis.

[0005]

However, there has not been any report on a propolis product which contains a wide range of physiologically active components of propolis and has excellent antioxidant activity.

Accordingly, the present invention has been made in view of the above circumstances, and it is an object of the present invention to provide a propolis product having a wide range of physiological activities possessed by propolis and having an antioxidant activity effective for preventing and improving age-related changes. As an issue.

[0007]

Means for Solving the Problems The present inventors have paid attention to the aqueous alcohol solution extraction method, and have repeatedly studied the antioxidant activity of a mixture of propolis extracts extracted with aqueous alcohol solutions having different amounts of water. I arrived. That is, the present invention relates to an antioxidatively active propolis product obtained by mixing a propolis extract obtained by extracting a propolis raw mass with two or more aqueous ethanol solutions having different amounts of water. Claim 1).

According to the invention having the above constitution, the hydrophilic component and the lipophilic component contained in propolis can be extracted, and not only the broad physiological activity of propolis can be expected, but also it has excellent antioxidant activity. It is possible to obtain a propolis product that is effective for preventing and improving aging. The term "propolis product" as used herein refers to a mixture of two or more propolis extracts themselves or a product obtained by formulating the mixture.

Further, in the invention according to claim 1, a propolis extract obtained by extracting a raw propolis mass with an aqueous ethanol solution having a water content of 5% by volume to less than 10% by volume, and a water content of And a propolis extract obtained by extraction with a 45% by volume to 55% by volume aqueous ethanol solution,
The present invention relates to an antioxidatively active propolis product characterized by being mixed at a volume mixing ratio of 4 to 6: 6 to 4 (claim 2).

[0010] In the invention described in claim 1 or claim 2, the propolis raw mass is eucalyptus or eucalyptus produced in Brazil.
It relates to an antioxidantly active propolis product characterized by being derived from alexin (claim 3). According to the invention of this configuration, since a propolis product containing a large amount of artepillin C is obtained, it is expected to induce apoptosis of cancer cells.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The raw material of propolis, which is the raw material of propolis extract, has various types and contents depending on the type of tree from which bees collect sap and the like. The original Brazilian propolis mass collected by bees from sap of Eucalyptus alexlen, which is excellent in quantity, is preferred. The original propolis lump is pulverized by a pulverizer depending on its size, but pulverization may be omitted if the lump is a small lump.

The original propolis mass is extracted using two or more aqueous ethanol solutions having different amounts of water. When two types of aqueous ethanol solutions having different amounts of water are used, the amount of one aqueous ethanol solution is 5% by volume to 10% by volume.
Is preferably less than 7% by volume to 9% by volume, and the amount of water in the other aqueous ethanol solution is 45% by volume.
-55% by volume, more preferably 48-53% by volume. The aqueous ethanol solution used for the extraction is preferably used in an amount of 3 to 6 times (ml) the amount (g) of the original propolis mass. The extraction operation may be performed at room temperature with stirring, but is preferably performed by heating at 65 ° C to 75 ° C. The extraction time is usually 1 to 3 hours, but can be appropriately increased or decreased.

When the above-mentioned extraction operation is completed, solid-liquid separation is carried out using a centrifugal separator immediately after cooling, if heating is not performed, and immediately after heating, to obtain a supernatant.

Next, the supernatant is concentrated by a reduced pressure operation. An excipient can be added to the concentrate. As the excipient, those usually used in the pharmaceutical field can be used, and examples thereof include dextrin, corn starch, rice flour, lactose, potato starch and the like.

The above concentrate is subjected to freeze-drying to obtain a solid. The obtained solid is pulverized using a hammer mill or the like to produce a powdered propolis extract.

The propolis product of the present invention is produced by mixing two or more propolis extracts extracted from aqueous ethanol solutions having different amounts of water produced as described above. Propolis extract extracted from 5% by volume to less than 10% by volume of an aqueous ethanol solution and 45% by volume of water
When mixing a propolis extract extracted from an aqueous ethanol solution of up to 55% by volume, it is preferable to mix the former and the latter so that the volume mixing ratio of the former and the latter becomes 4-6: 6-4. The mixing operation is performed sufficiently so that both are uniform.

The propolis product of the present invention produced as described above can be used in various forms, such as capsules and tablets usually used in the pharmaceutical field,
Alternatively, it can be used as a liquid preparation.

[0018]

EXAMPLES Next, the present invention will be described with reference to examples, but the present invention is not limited to the following examples.

1000 g of Brazilian propolis original mass derived from Eucalyptus alexin is placed in an extraction tank equipped with a stirrer, 4000 ml of an aqueous ethanol solution containing 9% by volume of water is added, and the mixture is heated to 60 ° C. with stirring. And extracted.

Next, after cooling the extract obtained above,
10,000 in bucket type centrifuge (manufactured by Takeda Machine Co., Ltd.)
r. p. For 15 minutes, solid-liquid separation was performed to obtain a supernatant. Furthermore, after the supernatant was concentrated under reduced pressure, an appropriate amount of rice flour was added and freeze-dried. The obtained solid was pulverized using a hammer mill to produce a propolis extract.

Further, a propolis extract was produced from a Brazilian propolis original mass derived from Eucalyptus alexulin by the same operation as above using an aqueous ethanol solution containing 50% by volume of water.

The propolis extract extracted from an aqueous ethanol solution having a water content of 9% by volume and the propolis extract extracted from an aqueous ethanol solution having a water content of 50% by volume, as described above, have a volume ratio shown in Table 1. To produce Examples 1 and 2 and Comparative Examples 1 and 2, respectively.

[0023]

[Table 1]

The superoxide scavenging activity of each of the examples and comparative examples was measured. The superoxide scavenging activity was measured by using an electron spin resonance (ESR) method. M. McCord and I.C. Fridovich
(J. Biol. Chem., 244,
6049 (1969)). Table 2 shows the results
It was shown to.

[0025]

[Table 2]

From Table 2, it can be seen that Examples 1 and 2 exhibited high superoxide scavenging activity, while Comparative Example 2
Had inferior superoxide scavenging activity. Further, Comparative Example 1 was superior to Comparative Example 2 in superoxide erasing activity, but was inferior to any of Examples. This proved that the superoxide scavenging activity was reduced when the mixing ratio of the propolis extract extracted with an aqueous ethanol solution containing a small amount of water was too small or too large.

[0027]

According to the present invention described in detail above, the following effects can be obtained. That is, the propolis product of the present invention
Since it contains sufficient hydrophilic and lipophilic components, it can be expected to have a wide range of physiological activities such as anticancer, antiallergic, antibacterial and antiviral activities, and skin diseases possessed by propolis, and is also excellent in antioxidant activity. Therefore, it is possible to provide a propolis product that is effective in preventing and improving aging changes.

 ──────────────────────────────────────────────────続 き Continued on the front page F term (reference) 4B018 MD78 ME06 MF01 MF02 4B041 LC10 LD06 LK35 LP05 4C087 AA01 AA02 AA04 BB22 MA02 MA52 ZC37

Claims (3)

[Claims] 1. An original propolis mass is prepared by mixing water with different amounts of water.
An antioxidatively active propolis product characterized by mixing propolis extracts obtained by extraction with at least one kind of aqueous ethanol solution. 2. A propolis extract obtained by extraction with an aqueous ethanol solution containing 5% by volume to less than 10% by volume of water and an aqueous ethanol solution containing 45% by volume to 55% by volume of water. The antioxidant-active propolis product according to claim 1, wherein the propolis extract obtained by the mixing is mixed at a mixing ratio of 4 to 6: 6 to 4 by volume. 3. The antioxidant-active propolis product according to claim 1, wherein the original propolis mass is derived from eucalyptus alexulin from Brazil.