CN108208781A - A kind of reparation hepatic injury, the Cordyceps militaris composition and preparation method and application of strengthen immunity - Google Patents
A kind of reparation hepatic injury, the Cordyceps militaris composition and preparation method and application of strengthen immunity Download PDFInfo
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- CN108208781A CN108208781A CN201711481585.3A CN201711481585A CN108208781A CN 108208781 A CN108208781 A CN 108208781A CN 201711481585 A CN201711481585 A CN 201711481585A CN 108208781 A CN108208781 A CN 108208781A
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- cordyceps militaris
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- 238000002360 preparation method Methods 0.000 title claims abstract description 17
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- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 2
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- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
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- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- HAWPXGHAZFHHAD-UHFFFAOYSA-N mechlorethamine Chemical compound ClCCN(C)CCCl HAWPXGHAZFHHAD-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of reparation hepatic injury, the Cordyceps militaris compositions and preparation method and application of strengthen immunity.Repair hepatic injury, the Cordyceps militaris composition of strengthen immunity is made of the raw material of following weight quota：Cordyceps militaris extract 9 20%, oligofructose 25 40%, lactitol 20 30%, maltodextrin 25 35%, black tea instant powder 2 3%, citric acid 0.01 0.1%, malic acid 0.01 0.1%, steviol glycoside 0.01 0.1%；Its preparation process is that fruiting bodies of cordyceps militaris is extracted, filtering, concentrates, drying process, obtains Cordyceps militaris extract, then be equipped with other raw materials and allocated, mixed, pelletized, dry, dispensed.The Cordyceps militaris composition of the present invention can play repair to mouse liver injury caused by cyclophosphamide, and there is significant immunological enhancement for the mouse of immunocompromised, wide application of the crowd, safety are without any side effects, Cordyceps militaris active ingredient is high and is easy to be absorbed, and further widen development of the Cordyceps militaris Related product in fields such as food, beverage exploitations.
Description
Technical field
The invention belongs to food, beverage or edible and medical fungi technical field, more particularly to a kind of reparation hepatic injury, enhancing are immune
The Cordyceps militaris composition and preparation method of power and application.
Background technology
With the improvement of living standards, the enhancing of people's health care consciousness, health products particularly medicine-food two-purpose resource obtains
Universal concern.The Cordyceps militaris (Cordyceps Militaris (L.) Link) of dietotherapeutic also known as Cordceps militaris, are fungi autoeciousness
It is China's tradition nourishing Chinese medicine in the entomogenous fungi complex that the pupa of insect is formed.Modern research shows that riched Cordyceps Militaris is more containing cordyceps sinensis
The multiple biological activities ingredient such as sugar, adenosine, cordycepin has cough-relieving, analgesia, decompression, antifatigue, antibacterial anti-inflammatory, anticancer, enhancing
A variety of physiological activity such as immunity, anti-aging.At present the metabolism of Cordyceps militaris phorozoon is concentrated mainly on around the research of Cordyceps militaris
Active constituent, pharmacological action level and the system research to Cordyceps militaris high-yield culture technique of product, and Cordyceps militaris is added deeply
The research of the Analysis on Biological Activity of work technology and finely processed product is still few, so that the application of Cordyceps militaris and Cordyceps militaris finely processed product
Limited to.
Cyclophosphamide also known as sendoxan, endoxan, cyclophosphamide or B-518 are that clinical treatment malignant tumour is the most frequently used
Alkylating agent represent drug, it is external inactive, but aoxidize generation intermediate product aldehyde phosphinylidyne through liver cell pigment P-450 in vivo
Amine, then decomposite in tumour cell phosphamide mustargen and alkanisation occurs for the DN of cell, cross link is formed, so as to inhibit
The growth and breeding of tumour cell.Cyclophosphamide has broad spectrum anticancer effect, significant in efficacy to malignant lymphoma, to multiple marrow
Knurl, acute lymphoblastic leukemia, oophoroma, breast cancer etc. are also effective.And in antineoplaston, cyclophosphamide can cause by
The adverse reactions such as the hepatic injury of Patient drug's property and immunosupress are controlled, influence the normal procedure of chemotherapy.
For this purpose, the present invention provides a kind of Cordyceps militaris composition and preparation method thereof, to solve to repair cyclophosphamide induction
Hepatic injury, and enhance immunity of organisms, to alleviate patient's physical condition, skeptophylaxis power hypofunction patient is treated, and
Process for stabilizing, efficiently production, have carried out practical application by Cordyceps militaris deep processing and its product well.
Invention content
The present invention for above-mentioned technical problem, provide it is a kind of repair hepatic injury, strengthen immunity Cordyceps militaris composition.
To realize the above-mentioned technical purpose, hepatic injury is repaired, the technical solution that the Cordyceps militaris composition of strengthen immunity is taken
For：
A kind of reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity, are made of the raw material of following mass fraction：Pupa worm
Careless extract 9-20%, oligofructose 25-40%, lactitol 20-30%, maltodextrin 25-35%, black tea instant powder 2-3%,
Citric acid 0.01-0.1%, malic acid 0.01-0.1%, steviol glycoside 0.01-0.1%.
Preferably, a kind of reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity, by following mass fraction
Raw material composition：Cordyceps militaris extract 9-10%, oligofructose 25-30%, lactitol 25-30%, maltodextrin 25-30%,
Black tea instant powder 2-2.5%, citric acid 0.01-0.05%, malic acid 0.01-0.05%, steviol glycoside 0.01-0.05%.
The present invention also provides a kind of above-mentioned reparation hepatic injury, the preparation methods of the Cordyceps militaris composition of strengthen immunity.
Repair hepatic injury, strengthen immunity Cordyceps militaris composition the technical solution that uses of preparation method for：
It is a kind of repair hepatic injury, strengthen immunity Cordyceps militaris composition preparation method, include the following steps：
1) fruiting bodies of cordyceps militaris is broken into after 10-50mm and be put into extractor, add in 20-30 times of fruiting bodies of cordyceps militaris matter
The water of amount extracts 2-4h at a temperature of 50-55 DEG C, using filter, concentration, dry, obtains Cordyceps militaris extract；
2) by the Cordyceps militaris extract and oligofructose, lactitol, maltodextrin, black tea instant powder, citric acid, apple
Acid, steviol glycoside allotment, mixing, granulation, dry, tabletting or not tabletting, packing.
Wherein, above-mentioned filtration step takes the mode of continuous centrifugal to be filtered, i.e., first after horizontal centrifuge centrifuges, then
It is centrifuged via disk centrifugal separator.
The horizontal centrifuge and disk centrifugal separator are respectively in rotating speed 4000-5000r/min and 6000-7000r/min item
Centrifugal filtration is carried out under part.
Cordyceps militaris composition the present invention also provides above-mentioned reparation hepatic injury, strengthen immunity is preparing food and beverage
The application of aspect
Relative to the prior art, the present invention has advantages below：
1) it is of the invention repair hepatic injury, strengthen immunity Cordyceps militaris composition for Mouse Liver caused by cyclophosphamide
Damage and immunocompromised have apparent reparation and humidification.
2) the reparation hepatic injury of the present invention, the reasonably combined raw material of Cordyceps militaris composition of strengthen immunity form, and mutually promote
Into, mutually collaboration, safety it is without any side effects, maltodextrin absorption easy to digest, steviol glycoside energy blood pressure lowering is hypoglycemic, at the same its
He also has the effect of preferable by auxiliary material for gastro-intestinal digestion, the proliferation for promoting enteron aisle beneficial bacteria etc..
3) reparation hepatic injury of the invention, the Cordyceps militaris composition applicability of strengthen immunity are extensive, and calorific value is low, for sugar
Urinating the patients such as disease, obesity still has good applicability.
4) reparations hepatic injury of the invention, strengthen immunity Cordyceps militaris composition reconstituted with water after without precipitate, it is not muddy,
Clean taste is easy to consumer and accepts extensively；Cordyceps militaris extract is water solubility, and wherein cordycepin isoreactivity ingredient is easily efficiently inhaled
It receives, product has been effectively ensured the effect of property.
5) it is of the invention repair hepatic injury, strengthen immunity Cordyceps militaris preparation method of composition process stabilizing, each process
It is connected unimpeded, granulation process not easy to knot groups, film forming is preferable, and production is efficient, promotes Cordyceps militaris deep processing series of products work very well
Industryization develops.
6) it is of the invention repair hepatic injury, strengthen immunity Cordyceps militaris composition, can be used in preparing food and beverage.
Specific embodiment
Embodiment 1
A kind of reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity, are made of the raw material of following mass fraction：Pupa worm
Careless extract 12.3%, oligofructose 33.8%, lactitol 21%, maltodextrin 31%, black tea instant powder 2%, citric acid
0.07%, malic acid 0.07%, steviol glycoside 0.06%.
A kind of above-mentioned reparation hepatic injury, the preparation method of the Cordyceps militaris composition of strengthen immunity, include the following steps：
1) fruiting bodies of cordyceps militaris is broken into after 10-50mm and be put into extractor, add in 20-30 times of fruiting bodies of cordyceps militaris matter
The water of amount extracts 3h at a temperature of 55 DEG C, through under the conditions of horizontal centrifuge rotating speed 4000r/min, disk centrifugal separator 7000r/min from
The heart filters, then concentrates, dries, and obtains Cordyceps militaris extract；
2) according to mass fraction, by 12.3% Cordyceps militaris extract and 33.8% oligofructose, 21% lactitol, 31% wheat
Bud dextrin, 2% black tea instant powder, 0.07% citric acid, 0.07% malic acid, 0.06% steviol glycoside allotment, mixing, granulation,
Dry, tabletting or not tabletting, packing to get.
Above-mentioned repairs hepatic injury, the Cordyceps militaris composition of strengthen immunity and boiling water according to 1：50 ratio is straight after reconstituting
It connects and drinks.
After above-mentioned reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity are mixed with flour and other food additives
Prepare health food.
Embodiment 2
A kind of reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity, are made of the raw material of following mass fraction：Pupa worm
Careless extract 10%, oligofructose 27.4%, lactitol 30%, maltodextrin 30%, black tea instant powder 2.5%, citric acid
0.02%, malic acid 0.03%, steviol glycoside 0.05%.
A kind of above-mentioned reparation hepatic injury, the preparation method of the Cordyceps militaris composition of strengthen immunity, include the following steps：
1) fruiting bodies of cordyceps militaris is broken into after 10-50mm and be put into extractor, add in 20-30 times of fruiting bodies of cordyceps militaris matter
The water of amount extracts 4h at a temperature of 50 DEG C, through under the conditions of horizontal centrifuge rotating speed 4500r/min, disk centrifugal separator 6500r/min from
The heart filters, then concentrates, dries, and obtains Cordyceps militaris extract；
2) according to mass fraction, by 10% Cordyceps militaris extract and 27.4% oligofructose, 30% lactitol, 30% malt
Dextrin, 2.5% black tea instant powder, 0.02% citric acid, 0.03% malic acid, 0.05% steviol glycoside allotment, mixing, granulation,
Dry, tabletting or not tabletting, packing to get.
Above-mentioned repairs hepatic injury, the Cordyceps militaris composition of strengthen immunity and boiling water according to 1：50 ratio is straight after reconstituting
It connects and drinks.
After above-mentioned reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity are mixed with flour and other food additives
Prepare health food.
Embodiment 3
A kind of reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity, are made of the raw material of following mass fraction：Pupa worm
Careless extract 20%, oligofructose 40%, lactitol 30%, maltodextrin 35%, black tea instant powder 3%, citric acid 0.1%,
Malic acid 0.1%, steviol glycoside 0.1%.
A kind of above-mentioned reparation hepatic injury, the preparation method of the Cordyceps militaris composition of strengthen immunity, include the following steps：
1) fruiting bodies of cordyceps militaris is broken into after 10-50mm and be put into extractor, add in 20-30 times of fruiting bodies of cordyceps militaris matter
The water of amount extracts 4h at a temperature of 55 DEG C, through under the conditions of horizontal centrifuge rotating speed 5000r/min, disk centrifugal separator 6000r/min from
The heart filters, then concentrates, dries, and obtains Cordyceps militaris extract；
2) according to mass fraction, by 20% Cordyceps militaris extract and 30% oligofructose, 21% lactitol, 25.7% malt
Dextrin, 3% black tea instant powder, 0.1% citric acid, 0.1% malic acid, the allotment of 0.1% steviol glycoside, mixing, granulation, it is dry,
Tabletting or not tabletting, packing to get.
Above-mentioned repairs hepatic injury, the Cordyceps militaris composition of strengthen immunity and boiling water according to 1：50 ratio is straight after reconstituting
It connects and drinks.
After above-mentioned reparation hepatic injury, the Cordyceps militaris composition of strengthen immunity are mixed with flour and other food additives
Prepare health food.
Experimental example
1 experimental method
1.1 experimental animals are grouped and administration
20 mouse half male and half females are separately raised.Male and female mouse is randomly divided into Normal group (10), model group respectively
(50).For model group mouse daily according to the dosage intraperitoneal injection of cyclophosphamide of 80mg/kg, Normal group uses physiological saline
Instead of continuously injecting 3d.Mouse after modeling is randomly divided into 5 groups, every group 10, respectively liver injury model group, positive control
Group (150mg/kg legalons), Cordyceps militaris composition high dose group (80mg/kg), middle dose group (40mg/kg), low dose group
(20mg/kg), mouse continuous gavage 14d, liver injury model group and the daily equivalent of Normal group give physiological saline.Last is given
The method that fasting takes exoculation to take blood afterwards for 24 hours after medicine collects mouse blood and centrifuges 10min under conditions of 4 DEG C of 3000r/min
- 20 DEG C of serum is collected to save backup.Separately -20 DEG C of liver homogenate (10%) supernatant is taken to save backup.
The administration of 1.2 mouse
After mice group, control group and model group intragastric administration on mice physiological saline, high, medium and low dose of Cordyceps militaris composition are given
Amount group is given 0.6mg, 0.5mg and 0.33mg Cordyceps militaris composition according to the conversion of Cordyceps militaris total daily dose and is carried out at gavage respectively
Reason.The equal gavage 14d of each group.
1.3 serum alts, AST, IL-2, TNF- ɑ are measured
Operating procedure is operated according to the requirement of kit.
The preparation of 1.4 liver homogenates and liver index, the measure of biochemical indicator
Mouse cervical dislocation dissects dead mouse after putting to death, and takes out mouse liver and with the physiological saline being pre-chilled
It is rinsed, then the moisture on internal organs is blotted with filter paper, weigh liver mass, 10% tissue is made with operation clip 0.1g livers
Homogenate after centrifuging 15min under conditions of 3000r/min, is collected -20 DEG C of supernatant and is saved backup.SOD, MDA in liver homogenate,
The measure of GSH, GSH-Px require to operate according to kit.
1.5 statistical analysis
For statistical analysis to experimental result using DPS7.05 editions softwares, all data use means standard deviation table
Show.
2 results and analysis
Influence of the 2.1 Cordyceps militaris compositions to hepatic injury mice serum AST, ALT activity
Cordyceps militaris composition causes cyclophosphamide influence result such as table 1 (hero), 1 of ALT and AST in hepatic injury mice serum
Shown in (female).
1 Cordyceps militaris composition of table causes cyclophosphamide the influence of hepatic injury mouse (hero) serum AST, ALT
*P<0.05,**P<0.01 compares with model group mouse；#P<0.05,##P<0.01 compares with normal group mouse.
Table 1 causes cyclophosphamide for Cordyceps militaris composition the influence of hepatic injury mouse (hero) serum AST, ALT.Model group is small
Mouse serum AST and ALT enzyme activity are respectively 62.43 ± 0.33 and 69.76 ± 0.48U/L, and AST the and ALT enzyme activity normally organized is only
For 18.21 ± 0.19 and 15.59 ± 0.31U/L, the difference between two groups reaches the pole level of signifiance (p<0.01), show ring phosphinylidyne
The intake of amine causes the damage of mouse liver cell, this also illustrates cyclophosphamide Liver Injury in Mice modeling success.Cordyceps militaris group
It is respectively 47.39 ± 0.48,34.21 ± 0.19 and 26.21 ± 0.19U/ to close the basic, normal, high dosage group mice serum AST enzyme activity of object
L, ALT enzyme activity are respectively 45.59 ± 0.31,38.30 ± 0.36 and 26.32 ± 0.36U/L, and pole is substantially less than model group (p<
0.01)。
2 Cordyceps militaris composition of table causes cyclophosphamide the influence of hepatic injury mouse (female) serum AST, ALT
*P<0.05,**P<0.01 compares with model group mouse；#P<0.05,##P<0.01 compares with normal group mouse.
Table 2 causes cyclophosphamide for Cordyceps militaris composition the influence of hepatic injury mouse (female) serum AST, ALT.Cordyceps militaris group
It is respectively 38.99 ± 0.38,29.10 ± 0.33 and 13.10 ± 0.33U/ to close the basic, normal, high dosage group mice serum AST enzyme activity of object
L, ALT enzyme activity are respectively 47.47 ± 0.31,32.47 ± 0.31 and 14.22 ± 0.37U/L, and pole is substantially less than model group (p<
0.01)。
The data of contrast table 1,2, as a result show：Cordyceps militaris composition can significantly reduce male and female mice serum AST,
The enzyme activity of ALT.But the enzyme activity of female mice serum AST, ALT are fast than male mice as the increasing of dosage reduces speed.
When high dose is 80mg/kg, female, male mice serum AST, ALT enzyme activity are respectively 13.10 ± 0.33,14.22 ± 0.37U/
L and 26.21 ± 0.19,26.32 ± 0.36U/L.
Influence of the 2.2 Cordyceps militaris compositions to mice serum IL-2, TNF- ɑ
Cordyceps militaris composition causes cyclophosphamide in the influence result such as table 3 of IL-2, TNF- ɑ in hepatic injury mice serum
Shown in (hero), 4 (female).
3 Cordyceps militaris composition of table causes hepatic injury mouse (hero) serum IL-2, TNF- to cyclophosphamideɑInfluence
*P<0.05,**P<0.01 compares with model group mouse；#P<0.05,##P<0.01 compares with normal group mouse.
Table 3 causes cyclophosphamide for Cordyceps militaris composition the influence of hepatic injury mouse (hero) serum IL-2, TNF- ɑ contents.
39.04 ± 0.75pg/mL of content poles of the IL-2 of Normal group are significantly higher than 25.19 ± 0.81pg/mL of model control group (p<
0.01), illustrate inject cyclophosphamide so as to cause immunosuppressed mice serum in IL-2 contents can decline, so as to make mouse
Immunocompetence weakening.Cyclophosphamide equally reduces the TNF-α content in Mice Body, the TNF-α content of model control group
Less than Normal group, there is extremely significant difference (p<0.01).High, medium and low group of TNF-α measured of Cordyceps militaris composition dosage
Content be respectively 15.56 ± 0.06,14.49 ± 0.49,12.85 ± 0.99pg/mL.Cordyceps militaris composition low dose group TNF-
The content of α is higher than model group, has extremely significant difference (p<0.01).The serum IL-2 of dosage group, TNF-α content are all with pupa
The raising of cordyceps sinensis composition dosage and increase, certain positive correlation is presented.
4 Cordyceps militaris composition of table causes hepatic injury mouse (female) serum IL-2, TNF- to cyclophosphamideɑInfluence
*P<0.05,**P<0.01 compares with model group mouse；#P<0.05,##P<0.01 compares with normal group mouse.
Table 4 causes cyclophosphamide for Cordyceps militaris composition the influence of hepatic injury mouse (female) serum IL-2, TNF- ɑ contents.
As a result it is similar with male mice.The serum IL-2 of dosage group, TNF-α content all increase with the raising of Cordyceps militaris composition dosage
Add, certain positive correlation is presented.Cordyceps militaris composition low dose group IL-2, TNF-α content higher than model control group, have
Pole level of signifiance difference (p<0.01).
The data of contrast table 3,4 are it is found that serum IL-2, TNF-α content and the Cordyceps militaris composition dosage of dosage group are presented
Certain positive correlation.Cordyceps militaris composition under same dosage group, cyclophosphamide is caused hepatic injury female mice serum IL-2,
TNF- ɑ contents are affected.
2.3 Cordyceps militaris compositions cause cyclophosphamide the influence of hepatic injury mouse liver even slurry Biochemical Indexes
The antioxidases such as SOD, CAT, GSH-Px are the important components of body anti-oxidative defense system, and Cordyceps militaris combines
Object causes the influence result of antioxidase and mda content such as table 5 (hero), 6 (female) in hepatic injury mouse liver even slurry to cyclophosphamide
It is shown.
5 Cordyceps militaris composition of table causes hepatic injury mouse (hero) hepatic tissue SOD, CAT, GSH-Px, GSH, MDA to cyclophosphamide
Influence
*P<0.05,**P<0.01 compares with model group mouse；#P<0.05,##P<0.01 compares with normal group mouse.
Table 5 causes cyclophosphamide for Cordyceps militaris composition hepatic tissue SOD, CAT, GSH- of acute hepatic injury mice (hero)
The influence of Px, GSH activity and MDA contents.Model group mouse liver SOD, CAT, GSH-Px, GSH activity is respectively 305.86 ±
0.40th, 78.41 ± 0.65,230.30 ± 1.14 and 0.35 ± 0.09U/mg pro, pole are substantially less than normal group of (p<0.01),
The lipid peroxidation product MDA contents of model group are 3.82 ± 0.01nmol/mg pro, and pole is significantly higher than normal group of (p<
0.01).Show that cyclophosphamide intake causes the damage of mouse (hero) liver Antioxidative Defense System.Low dose of Cordyceps militaris composition
In amount group mouse liver SOD, CAT, GSH-Px, GSH activity be respectively 346.96 ± 0.78,85.81 ± 0.74,341.68 ±
1.74 and 3.70 ± 0.07U/mg pro, pole are significantly higher than model group (p<0.01), the MDA of Cordyceps militaris composition low dose group
Content is 1.94 ± 0.01nmol/mg pro, and pole is substantially less than model group (p<0.01).Test group Cordyceps militaris composition dosage is got over
Height, it is also higher that antioxidant enzyme levels rise the degree declined with MDA contents.150mg/kg legalons also can pole significantly improve it is anti-
Oxidase active (p<0.01) MDA contents (p, is reduced<0.01).
6 Cordyceps militaris composition of table causes hepatic injury mouse (female) hepatic tissue SOD, CAT, GSH-Px, GSH, MDA to cyclophosphamide
Influence
*P<0.05,**P<0.01 compares with model group mouse；#P<0.05,##P<0.01 compares with normal group mouse.
Table 6 causes cyclophosphamide for Cordyceps militaris composition hepatic tissue SOD, CAT, GSH- of acute hepatic injury mice (female)
The influence of Px, GSH activity and MDA contents.Model group mouse liver SOD, CAT, GSH-Px, GSH activity be respectively (334.11 ±
1.08), (65.79 ± 0.31), (454.11 ± 1.21) and (5.44 ± 0.42) U/mg pro, pole are substantially less than normal group of (p
<0.01), the lipid peroxidation product MDA contents of model group are (3.81 ± 0.01) nmol/mg pro, and pole is significantly higher than normally
Group (p<0.01).Test group Cordyceps militaris composition dosage is higher, and the degree that antioxidase activity raising declines with MDA contents is also got over
Greatly.
Contrast table 5, table 6 are found：No matter male and female mouse, Cordyceps militaris composition can significantly improve cyclophosphamide induction it is small
Mouse liver SOD, CAT, GSH-Px, GSH activity, reduce MDA contents, show that Cordyceps militaris composition can improve the anti-oxidant energy of body
Power reduces the generation of lipid peroxidation.In high dose group, SOD, CAT and GSH-Px activity of male mice hepatic tissue than
The activity of female mice hepatic tissue is high, and MDA contents are lower than female mice hepatic tissue.
3 brief summaries
In above experiment, the mouse liver injury that the Cordyceps militaris composition of the embodiment of the present invention 2 induces cyclophosphamide is studied
Repair.By Kunming mouse be randomly divided into normal group, it is model group, Cordyceps militaris composition high dose group, middle dose group, low
Dosage group gives various dose Cordyceps militaris composition using intraperitoneal injection of cyclophosphamide structure mouse liver injury model and gavage,
Liver, spleen is taken to calculate organ index；Measure glutamic-pyruvic transaminase (ALT), paddy in each group mice serum respectively using RNA isolation kit
Superoxides discrimination in careless transaminase (AST), interleukin 2 (IL-2), tumor necrosis factor (TNF- ɑ) and liver tissue homogenate
It is horizontal to change enzyme (SOD), catalase (CAT), peroxidase (GSH-Px), malonaldehyde (MDA).Experimental result is shown：Pupa
Cordyceps sinensis composition high dose group extremely significantly (P<0.01) reduce cyclophosphamide cause hepatic injury mice serum glutamic-pyruvic transaminase (ALT),
The enzyme activity of glutamic-oxalacetic transaminease (AST), significantly extremely (P<0.01) Serum interleukin-8 (IL-2), tumor necrosis factor are improved
The content of (TNF- ɑ), significantly extremely (P<0.01) superoxide dismutase (SOD), catalase in liver tissue homogenate are improved
(CAT), peroxidase (GSH-Px) enzyme activity, significantly extremely (P<0.01) malonaldehyde (MDA) content is reduced.Wherein, Cordyceps militaris group
Conjunction object influences ALT, AST enzyme activity in female mice serum and IL-2, the content of TNF- ɑ bigger than male mice.Show
Cordyceps militaris composition has repair to mouse liver injury caused by cyclophosphamide, and significantly increases exempting from for immunosuppressed mice
Epidemic disease ability.
Embodiment described above only expresses several preferred embodiments of the present invention, and description is more specific and detailed,
But therefore it cannot be interpreted as the limitation to the scope of the claims of the present invention.It should be pointed out that the ordinary skill people for this field
For member, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the present invention's
Protection domain.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (6)
1. it is a kind of repair hepatic injury, strengthen immunity Cordyceps militaris composition, which is characterized in that the Cordyceps militaris composition by with
The raw material composition of lower mass fraction：Cordyceps militaris extract 9-20%, oligofructose 25-40%, lactitol 20-30%, malt paste
Smart 25-35%, black tea instant powder 2-3%, citric acid 0.01-0.1%, malic acid 0.01-0.1%, steviol glycoside 0.01-
0.1%.
2. a kind of reparation hepatic injury according to claim 1, the Cordyceps militaris composition of strengthen immunity, which is characterized in that
The Cordyceps militaris composition is made of the raw material of following mass fraction：Cordyceps militaris extract 9-15%, oligofructose 25-30%,
Lactitol 25-30%, maltodextrin 25-30%, black tea instant powder 2-2.5%, citric acid 0.01-0.05%, malic acid 0.01-
0.05%, steviol glycoside 0.01-0.05%.
3. repaired described in a kind of claims 1 or 2 hepatic injury, strengthen immunity Cordyceps militaris composition preparation method, it is special
Sign is, includes the following steps：
1) fruiting bodies of cordyceps militaris is broken into after 10-50mm and be put into extractor, 20-30 times of fruiting bodies of cordyceps militaris quality of addition
Water extracts 2-4h at a temperature of 50-55 DEG C, using filter, concentration, dry, obtains Cordyceps militaris extract；
2) by the Cordyceps militaris extract and oligofructose, lactitol, maltodextrin, black tea instant powder, citric acid, malic acid,
Steviol glycoside allotment, mixing, granulation, dry, tabletting or not tabletting, packing.
4. it is according to claim 3 it is a kind of repair hepatic injury, strengthen immunity Cordyceps militaris composition preparation method,
It being characterized in that, the filtration step takes the mode of continuous centrifugal to be filtered, i.e., first after horizontal centrifuge centrifuges, then via
Disk centrifugal separator is centrifuged.
5. it is according to claim 4 it is a kind of repair hepatic injury, strengthen immunity Cordyceps militaris composition preparation method,
It is characterized in that, the horizontal centrifuge and disk centrifugal separator are respectively in rotating speed 4000-5000r/min and 6000-7000r/min item
Centrifugal filtration is carried out under part.
6. repair hepatic injury described in a kind of claims 1 or 2, the Cordyceps militaris composition of strengthen immunity is preparing food and beverage
The application of aspect.
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