CN104800174B - A kind of raspberry polysaccharide buccal tablet and application thereof - Google Patents
A kind of raspberry polysaccharide buccal tablet and application thereof Download PDFInfo
- Publication number
- CN104800174B CN104800174B CN201510169861.7A CN201510169861A CN104800174B CN 104800174 B CN104800174 B CN 104800174B CN 201510169861 A CN201510169861 A CN 201510169861A CN 104800174 B CN104800174 B CN 104800174B
- Authority
- CN
- China
- Prior art keywords
- raspberry
- polysaccharide
- raspberry polysaccharide
- group
- purposes according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides a kind of raspberry polysaccharide buccal tablet, it is prepared from by the supplementary material of following weight proportion：20~50 parts of raspberry polysaccharide, 10~30 parts of microcrystalline cellulose, 1~15 part of citric acid, 5~20 parts of Icing Sugar, 0.1~3 part of lubricant, appropriate adhesive.Present invention also offers the purposes of the lozenge.Present invention research is found, raspberry polysaccharide has good active anticancer, can effectively reduce the toxic side effect of chemotherapeutics, and also has hypoglycemic and anti-fatigue effect, therefore, the lozenge that is prepared by active component of raspberry polysaccharide of the present invention has good prospect of the application and social effect.
Description
Technical field
The invention belongs to medical sci-tech field, and in particular to a kind of raspberry polysaccharide buccal tablet and preparation method thereof, further relate to tree
The purposes of certain kind of berries polysaccharide buccal tablet.
Background technology
Malignant tumour is still the major disease of serious threat human life.Nearest statistics shows that China is new every year
It was found that the people of cancer patient about 20,000,000, wherein 1,500,000 people die from cancer.The death toll of cancer accounts for the 1/5 of total death toll.
Raspberry (Rubus idaeus L.) also known as Rubus corchorifolius, raspberry, raspberry etc., belong to rose family rubus.Raspberry master
Northern Hemisphere temperate zone and frigid zone are distributed in, its fruit is starches small aggregate fruit more, and taste sweet tea is slightly sour.Raspberry contains polysaccharide, organic acid, Huang
Ketone, anthocyanidin, the various active composition such as polyphenol, vitamin, mineral element, volatile oil, tannin, with high nutritive value and
Medical care effect, is described as " gold fruit ".At present, the raspberry product on domestic market is still based on foodstuff, such as with
Raspberry is fruit juice, jam, fruit wine of primary raw material etc..And raspberry active composition is extracted, and these active ingredients are applied to guarantor
Strong product or even the example in medical sci-tech field are considerably less.
The report of stomach cancer can effectively be suppressed by having patent to disclose Bramble gallogen class extract at present.However, right at present
Raspberry polysaccharide is but without research in this respect.
The content of the invention
The invention reside in a kind of raspberry polysaccharide buccal tablet is provided, another object of the present invention is to provide the purposes of the lozenge.
Specifically, the invention provides a kind of raspberry polysaccharide buccal tablet, it be by following weight proportion supplementary material prepare and
Into：
20~50 parts of raspberry polysaccharide, 10~30 parts of microcrystalline cellulose, 1~15 part of citric acid, 5~20 parts of Icing Sugar, lubricant
0.1~3 part, appropriate adhesive.
Further, it is prepared from by the supplementary material of following weight proportion：
30~45 parts of raspberry polysaccharide, 8 parts of microcrystalline cellulose, 1 part of citric acid, 15~20 parts of Icing Sugar, 0.1~3 part of lubricant,
Appropriate adhesive.
Wherein, the lubricant is selected from Macrogol 6000 or magnesium stearate；Further, described adhesive is selected from PVP
Ethanol solution.
Wherein, the preparation method of the lozenge is as follows：
(1) supplementary material is taken, is mixed in proportion, suitable amount of adhesive softwood, granulation is added；
(2) lubricant is added in particle, tabletting is carried out, obtains raspberry polysaccharide buccal tablet.
Wherein, in the raspberry polysaccharide, purity of polysaccharide is more than 50%；Further, purity of polysaccharide is more than 80%；More
Further, purity of polysaccharide is more than 85%；Preferably, purity of polysaccharide is 85~95%w/w.
Wherein, the raspberry polysaccharide is prepared using water extraction and alcohol precipitation method；Further, the water extraction and alcohol precipitation method is specifically grasped
Make as follows：Take after dry raspberry, petroleum ether or ether defatting, extracted with 75~95%v/v of concentration ethanol, filtering, filter residue adds water
Extract, after water intaking extract concentration, plus ethanol to alcohol content reaches 75%~85%, cools down, stands, it is that raspberry is more to take solid content
Sugar.Wherein, remove seed or non-remove seed before raspberry is extracted.
Wherein, the raspberry is the fruit of rose family rubus plant；Further, the raspberry be selected from raspberry or
Black raspberry.Antitumor or prevention or treatment caused by chemotherapeutic medicines poison are being prepared present invention also offers above-mentioned raspberry polysaccharide buccal tablet
Purposes in food, medicine or the health products of side effect.
Further, the tumour is melanoma, nasopharyngeal carcinoma, liver cancer, lung cancer, glioma, thyroid cancer, pancreas
Cancer, the cancer of the esophagus, colon cancer, oophoroma or prostate cancer.
In the present invention, the toxic side effect is organ damage, in the specific embodiment of the invention, and the organ is liver, spleen
Or kidney.
Wherein, the chemotherapeutics is selected from taxol, cis-platinum or/and docetaxel.
Wherein, the chemotherapeutics uses injection type.Wherein, the injection type can be selected from hypodermic injection, muscle
Usual way in the various tumor therapeutic procedures such as injection or intravenous injection.
Hypoglycemic or antifatigue medicine, health products or food are being prepared present invention also offers above-mentioned raspberry polysaccharide buccal tablet
In purposes.
Further, the medicine, health products or food are prevention or medicine, the health care for the treatment of I types or type ii diabetes
Product or food.
Further, the medicine, health products or food be improve the medicine of dyslipidemia in diabetes, health products or
Food.
Further, the medicine, health products or food are reduction diabetic's TC, TG and LDL levels, are improved
Medicine, health products or the food of HDL levels.
Further, the medicine, health products or food are reduction serum urea nitrogen, lactic acid, creatine kinase level, are improved
Blood glucose, the medicine of lactate dehydrogenase levels, health products or food.
The present invention finally found that raspberry polysaccharide to rising using the internal antitumous effect of mice with tumor model evaluation raspberry polysaccharide
Coming from skin, incidence, brain, thyroid gland, pancreas, oesophagus, colon or the rectum, ovary, the tumour of prostate of people has well
Inhibitory action, and act on it is close with chemotherapeutics, toxic side effect is smaller, for treatment or prevention tumour provide new choosing
Select.
Human body can produce toxic side effect, such as liver, kidney or/and splenic injury really after using chemotherapeutics, this
Invention research is found, can effectively reduce the toxic side effects such as the above-mentioned organ damage that chemotherapeutics is brought using raspberry polysaccharide, very
The antitumaous effect of chemotherapeutics can extremely be strengthened, new selection is provided for the clinical application of cancer patient.
To sum up, present invention research finds that raspberry polysaccharide has good active anticancer, can effectively reduce chemotherapeutics
Toxic side effect, and also there is hypoglycemic and anti-fatigue effect, therefore, what the present invention was prepared by active component of raspberry polysaccharide contains
Piece has good prospect of the application and social effect.
Brief description of the drawings
The raspberry polysaccharide of accompanying drawing 1 is to melanin tumour b16 F10 mouse transplanting tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 2 is to human nasopharyngeal carcinoma CNE nude mouse xenograft tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 3 is to human glioma U87 nude mouse xenograft tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 4 is to human thyroid carcinomas SW-579 nude mouse xenograft tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 5 is to human pancreas cancer SW-1990 nude mouse xenograft tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 6 is to human esophagus cancer Ec109 nude mouse xenograft tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 7 is to human colon carcinoma HT-29 nude mouse xenograft tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 8 is to human ovarian cancer SK-OV-3 nude mouse xenograft tumor growth inhibition effects；
The raspberry polysaccharide of accompanying drawing 9 is to human prostata cancer DU-145 nude mouse xenograft tumor growth inhibition effects.
10 docetaxels of accompanying drawing~raspberry polysaccharide is to melanin tumour b16 F10 mouse transplanting tumor growth inhibition effects；
11 cis-platinums of accompanying drawing~raspberry polysaccharide is to human lung cancer H460 nude mouse xenograft knurl growth inhibition effects；
Suppression of 12 taxols of the accompanying drawing~raspberry polysaccharide to the growth of the nude mouse xenograft tumor of human liver cancer Bel~7402 is made
With；
13 chemotherapeutics of accompanying drawing~raspberry polysaccharide combines the influence to tumor-bearing mice liver organization form；
14 chemotherapeutics of accompanying drawing~raspberry polysaccharide combines the influence to tumor-bearing mice renal tissue form；
15 chemotherapeutics of accompanying drawing~raspberry polysaccharide combines the influence to C57BL/6 mouse spleen indexes.
Embodiment
The raspberry polysaccharide used in the specific embodiment of the invention is prepared into using conventional Polyose extraction, way of purification
Arrive, concrete operations are as follows in the present invention：
It is prepared by raspberry polysaccharide：Dry raspberry is taken, is crushed after remove seed, plus 5 times of petroleum ether (60~90 DEG C of boiling range) degreasings, eliminate stone
75~95%v/v ethanol is added after oily ether in 60 DEG C of backflows, the filter residue after filtering is added into 10 times of pure water ultrasonic extraction (power
60W, temperature：80 DEG C, processing time：100min), centrifuging and taking supernatant, filter residue is extracted twice again by old terms, and merging filtrate subtracts
Pressure is concentrated into the 1/4 of original volume, adds 95%v/v ethanol, makes solution alcohol content up to 75%, 24h, filtering are stood in 4 degrees Celsius
Filter residue is collected, filter residue dries to obtain raspberry polysaccharide.
After measured, the raspberry polysaccharide yield that prepared by the present invention is 10.4%~11.6%, is obtained through Phenol-sulphate acid method detection
Purity of polysaccharide is 90.8%~91.2%.
In various embodiments of the present invention, raspberry polysaccharide dosage is in terms of polysaccharide.
Embodiment 1
The inhibitory action that raspberry polysaccharide grows to melanin tumour b16 F10 mouse xenograft tumors
Take the logarithm the melanoma cells B16F10 in growth period, is aseptically prepared into 5 × 107/ ml cell suspensions,
C57BL/6 right side of mice armpits are inoculated in 0.1ml subcutaneous.Vernier caliper measurement mice-transplanted tumor diameter is used after 5 days, tumour is treated
Grow to 60-90mm3Animal is grouped at random afterwards.Use the method in measurement knurl footpath, the antitumous effect of dynamic observation subject medicine.
The pendulous frequency of diameter of tumor is every 2 days 1 time, and measurement every time also needs to weigh mouse weight simultaneously.Packet and administering mode are as follows：It is negative
Control group injects normal saline, 1 time a day.Positive controls (docetaxel) are using hypodermic injection, 10mg/kg, every three
It is administered once.The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg, 200mg/kg, 400mg/kg, using gastric infusion,
1 time a day.
Gross tumor volume calculation formula：TV=0.52 × a × b2, wherein a, b represent length and width respectively.According to the result meter of measurement
Calculate relative tumour volume.The evaluation index of antitumor activity is Relative tumor proliferation rate T/C (%), calculation formula：T/C (%)
=TRTV/CRTV× 100%, TRTV：Treatment group RTV；CRTV：Negative control group RTV.
The inhibitory action that the raspberry polysaccharide of table 1. grows to melanin tumour b16 F10 mouse xenograft tumors
As a result：It is shown in Table 1 and Fig. 1, suppressions of the positive control docetaxel 10mg/kg to melanin tumour b16 F10 mice-transplanted tumors
Ratio of outflow is 66.49%.The low middle high dose group of raspberry polysaccharide is respectively to the tumour inhibiting rate of melanin tumour b16 F10 mice-transplanted tumors
7.56%, 24.32 and 60.95%.Compared with negative control group, docetaxel and raspberry high dose are small to melanin tumour b16 F10
Inhibitory action (* P ＜ 0.05, * * P ＜ 0.01) of the growth with conspicuousness of mouse transplantable tumor.But docetaxel toxicity is larger, move
Substantially, animal has death to thing Body weight loss in experimentation.Raspberry polysaccharide has no significant effect to the body weight of experimental animal, has no bright
Aobvious toxicity.·
Embodiment 2
Raspberry polysaccharide is to human nasopharyngeal carcinoma CNE nude mouse xenograft tumor growth inhibition tests
Take the logarithm the tumour cell in growth period, be aseptically prepared into 5 × 107/ ml cell suspensions, are connect with 0.1ml
Plant the armpit on the right side of nude mice subcutaneous.With vernier caliper measurement transplanted tumor in nude mice diameter, treat tumour growth to 100-200mm3After move
Thing is grouped at random.Use the method in measurement knurl footpath, the antitumous effect of dynamic observation raspberry polysaccharide.The pendulous frequency of diameter of tumor
For every 2 days 1 time, measurement every time also needs to weigh mouse weight simultaneously.
Administering mode is as follows：Negative control group injects normal saline, 1 time a day；Cis-platinum group 10mg/kg, using skin
Lower injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg, 200mg/kg, 400mg/kg, use
Gastric infusion, 1 time a day.Gross tumor volume calculates be the same as Example 1
The inhibitory action that the raspberry polysaccharide of table 2. grows to human nasopharyngeal carcinoma CNE nude mouse xenograft tumors
As a result：2 and Fig. 2 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human nasopharyngeal carcinoma CNE transplanted tumor in nude mice
70.38%；The basic, normal, high dosage group of raspberry polysaccharide is respectively 39.77% to the tumour inhibiting rate of human nasopharyngeal carcinoma CNE transplanted tumor in nude mice,
52.36%, 65.18%.But toxicity of cisplatin is larger, the weight of animals declines substantially, and animal has death in experimentation.And raspberry is more
Sugar influences on experimental animal body weight without conspicuousness.
Therefore, raspberry polysaccharide shows human nasopharyngeal carcinoma CNE transplanted tumor in nude mice growth inhibition test results, with negative control group
Compare, growth inhibitory action (* * P ＜ with pole conspicuousness of the raspberry polysaccharide high dose group to human nasopharyngeal carcinoma CNE transplantable tumors
0.01), growth inhibitory action (* P ＜ with conspicuousness of the raspberry polysaccharide middle dose group to human nasopharyngeal carcinoma CNE transplantable tumors
0.05).Compared with positive control cis-platinum, raspberry polysaccharide has no significant effect to the body weight of experimental animal, has no that obvious poison is secondary anti-
Should.
Embodiment 3
The suppression experiment that raspberry polysaccharide grows to human glioma U87 nude mouse xenograft tumors
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injects normal saline, daily 1
It is secondary；Taxol group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/
Kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibitory action that the raspberry polysaccharide of table 3. grows to human glioma U87 nude mouse xenograft tumors
As a result：3 and Fig. 3 are shown in Table, taxol 10mg/kg groups are to the tumour inhibiting rate of human glioma U87 transplanted tumor in nude mice
73.11%；The high, medium and low dosage group of raspberry polysaccharide to the tumour inhibiting rate of human glioma U87 transplanted tumor in nude mice respectively up to 78.19%,
76.21%, 69.73%.But taxol toxicity is larger, the weight of animals declines, and animal has death in experimentation.And raspberry polysaccharide
There is no conspicuousness influence to nude mice body weight.
Therefore, raspberry polysaccharide shows human glioma U87 transplanted tumor in nude mice growth inhibition test results, with negative control
Group is compared, and growth of the high, medium and low dosage group of raspberry polysaccharide to human glioma U87 transplantable tumors is respectively provided with the suppression of pole conspicuousness
Act on (* * P ＜ 0.01).Compared with positive controls taxol, raspberry polysaccharide has not significant impact to the body weight of experimental animal,
Have no obvious toxicity.
Embodiment 4
Raspberry polysaccharide human thyroid carcinomas SW-579 nude mouse xenograft tumor growth inhibition tests
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Dosage regimen is as follows：Negative control group injects equivalent
Physiological saline, 1 time a day；5 FU 5 fluorouracil group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle height of raspberry polysaccharide
Dosage group is respectively 100mg/kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibitory action that the raspberry polysaccharide of table 4. grows to human thyroid carcinomas SW-579 nude mouse xenograft tumors
As a result：4 and Fig. 4 are shown in Table, 5-Fu (5 FU 5 fluorouracil) 10mg/kg groups are to human thyroid carcinomas SW-579 transplanted tumor in nude mice
Tumour inhibiting rate be 69.26%；Tumour inhibiting rate of the high, medium and low dosage group of raspberry polysaccharide to human thyroid carcinomas SW-579 transplanted tumor in nude mice
Respectively up to 70.81%, 62.35%, 57.98%.But 5-Fu toxicity is larger, the weight of animals declines, and there is extremely animal in experimentation
Die.And raspberry polysaccharide does not have conspicuousness influence to nude mice body weight.
Therefore, raspberry polysaccharide shows human thyroid carcinomas SW-579 transplanted tumor in nude mice growth inhibition test results, with feminine gender
Control group is compared, and the high, medium and low dosage group group of raspberry polysaccharide extremely shows to being respectively provided with for growth of human thyroid carcinomas SW-579 transplantable tumors
The inhibitory action (* * P ＜ 0.01) of work property.Compared with positive controls 5-Fu, raspberry polysaccharide is not bright to the body weight of experimental animal
Development rings, and has no obvious toxicity.
Embodiment 5
Raspberry polysaccharide is to human pancreas cancer SW-1990 nude mouse xenograft tumor growth inhibition tests
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injects normal saline, daily 1
It is secondary；5 FU 5 fluorouracil group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively
100mg/kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibitory action that the raspberry polysaccharide of table 5. grows to human pancreas cancer SW-1990 nude mouse xenograft tumors
As a result：5 and Fig. 5 are shown in Table, 5-Fu 10mg/kg groups are to the tumour inhibiting rate of human pancreas cancer SW-1990 transplanted tumor in nude mice
65.26%；The high, medium and low dosage group of raspberry polysaccharide reaches respectively to the tumour inhibiting rate of human pancreas cancer SW-1990 transplanted tumor in nude mice
69.08%, 53.82%, 45.31%.But 5-Fu toxicity is larger, the weight of animals declines, and animal has death in experimentation.And set
Certain kind of berries polysaccharide does not have conspicuousness influence to nude mice body weight.
Therefore, raspberry polysaccharide shows human pancreas cancer SW-1990 transplanted tumor in nude mice growth inhibition test results, right with feminine gender
Compared according to group, growth of the raspberry polysaccharide high dose group to human pancreas cancer SW-1990 transplantable tumors there are inhibitory action (the * * of pole conspicuousness
P ＜ 0.01), growth of the raspberry polysaccharide middle dose group to human pancreas cancer SW-1990 transplantable tumors has inhibitory action (the * P ＜ of conspicuousness
0.05).Compared with positive controls 5-Fu, raspberry polysaccharide has not significant impact to the body weight of experimental animal, has no obvious poison
Side reaction.
Embodiment 6
Raspberry polysaccharide is to human esophagus cancer Ec109 nude mouse xenograft tumor growth inhibition tests
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injects normal saline, daily 1
It is secondary；Taxol group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/
Kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibitory action that the raspberry polysaccharide of table 6. grows to human esophagus cancer Ec109 nude mouse xenograft tumors
As a result：6 and Fig. 6 are shown in Table, taxol 10mg/kg groups are to the tumour inhibiting rate of human esophagus cancer Ec109 transplanted tumor in nude mice
69.76%；The high, medium and low dosage group of raspberry polysaccharide to the inhibitory rate 67.55% of human esophagus cancer Ec109 transplanted tumor in nude mice,
50.03%, 40.21%.But taxol toxicity is larger, the weight of animals declines substantially, there is death in process of the test.And raspberry polysaccharide
There is no conspicuousness influence to nude mice body weight.
Therefore, raspberry polysaccharide shows human esophagus cancer Ec109 transplanted tumor in nude mice growth inhibition test results, with negative control
Group is compared, and growth of the raspberry polysaccharide high dose group to human esophagus cancer Ec109 transplantable tumors has inhibitory action (the * * P ＜ of pole conspicuousness
0.11), growth of the raspberry polysaccharide middle dose group to human esophagus cancer Ec109 transplantable tumors has inhibitory action (the * P ＜ of conspicuousness
0.05).Compared with positive controls taxol, raspberry polysaccharide has not significant impact to the body weight of experimental animal, has no obvious
Toxicity.
Embodiment 7
The suppression experiment that raspberry polysaccharide grows to human colon carcinoma HT-29 nude mouse xenograft tumors
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injects normal saline, daily 1
It is secondary；Taxol group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/
Kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibitory action that the raspberry polysaccharide of table 7. grows to human colon carcinoma HT-29 nude mouse xenograft tumors
As a result：7 and Fig. 7 are shown in Table, taxol 10mg/kg groups are to the tumour inhibiting rate of human colon carcinoma HT-29 transplanted tumor in nude mice
68.23%；The high, medium and low dosage group of raspberry polysaccharide is respectively 62.88% to the tumour inhibiting rate of human colon carcinoma HT-29 transplanted tumor in nude mice,
53.13%, 45.33%.But taxol toxicity is larger, the weight of animals declines substantially, there is death in process of the test.And raspberry polysaccharide
There is no conspicuousness influence to nude mice body weight.
Therefore, raspberry polysaccharide shows human colon carcinoma HT-29 transplanted tumor in nude mice growth inhibition test results, with negative control
Group is compared, and growth of the raspberry polysaccharide high dose group to human colon carcinoma HT-29 transplantable tumors has inhibitory action (the * * P ＜ of pole conspicuousness
0.01), growth of the raspberry polysaccharide middle dose group to human colon carcinoma HT-29 transplantable tumors has inhibitory action (the * P ＜ of conspicuousness
0.05).Compared with positive controls taxol, raspberry polysaccharide has not significant impact to the body weight of experimental animal, has no obvious
Toxicity.
Embodiment 8
The suppression experiment of raspberry polysaccharide human ovarian cancer SK-OV-3 nude mouse xenograft tumors growth
Specific embodiment is with reference to embodiment 2.Administering mode is as follows：Negative control group injects normal saline, daily 1
It is secondary；Cis-platinum group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg,
200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibitory action that the raspberry polysaccharide of table 8. grows to human ovarian cancer SK-OV-3 nude mouse xenograft tumors
As a result：8 and Fig. 8 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human ovarian cancer SK-OV-3 transplanted tumor in nude mice
The 68.24% high, medium and low dosage group of raspberry polysaccharide is respectively to the tumour inhibiting rate of human ovarian cancer SK-OV-3 transplanted tumor in nude mice
70.13%, 59.87%, 48.08%.But toxicity of cisplatin is larger, the weight of animals declines substantially, there is death in process of the test.And set
Certain kind of berries polysaccharide does not have conspicuousness influence to nude mice body weight.
Therefore, raspberry polysaccharide shows human ovarian cancer SK-OV-3 transplanted tumor in nude mice growth inhibition test results, right with feminine gender
Compared according to group, raspberry polysaccharide height, growth of the middle dose group to human ovarian cancer SK-OV-3 transplantable tumors have the inhibitory action of pole conspicuousness
(* * P ＜ 0.01), growth of the raspberry polysaccharide low dose group to human ovarian cancer SK-OV-3 transplantable tumors has the inhibitory action (* of conspicuousness
P ＜ 0.05).Compared with positive controls cis-platinum, raspberry polysaccharide has not significant impact to the body weight of experimental animal, has no obvious
Toxicity.
Embodiment 9
The suppression experiment that raspberry polysaccharide grows to human prostata cancer DU-145 nude mouse xenograft tumors
Specific embodiment is with reference to embodiment 2.Administering mode is as follows：Negative control group injects normal saline, daily 1
It is secondary；Cis-platinum group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg,
200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibitory action that the raspberry polysaccharide of table 9. grows to human prostata cancer DU-145 nude mouse xenograft tumors
As a result：9 and Fig. 9 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human prostata cancer DU-145 transplanted tumor in nude mice
71.38%；The high, medium and low dosage group of raspberry polysaccharide is respectively to the tumour inhibiting rate of human prostata cancer DU-145 transplanted tumor in nude mice
75.31%, 55.63%, 48.27%.But toxicity of cisplatin is larger, the weight of animals declines substantially, there is death in process of the test.And set
Certain kind of berries polysaccharide does not have conspicuousness influence to nude mice body weight.
Therefore, raspberry polysaccharide shows human prostata cancer DU-145 transplanted tumor in nude mice growth inhibition test results, with feminine gender
Control group is compared, and growth of the raspberry polysaccharide high dose group to human prostata cancer DU-145 transplantable tumors has the inhibitory action of pole conspicuousness
In (* * P ＜ 0.01), raspberry polysaccharide, growth of the low dose group to human prostata cancer DU-145 transplantable tumors have conspicuousness suppression make
With (* P ＜ 0.05).Compared with positive controls cis-platinum, raspberry polysaccharide influences on the body weight of experimental animal without conspicuousness, has no bright
Aobvious toxicity.
Main component tree of the present invention in terms of tumor-inhibiting action, attenuation synergistic and immunological regulation three to raspberry polysaccharide buccal tablet
Certain kind of berries polysaccharide has carried out the pharmacodynamic study of adjuvant for chemotherapy of tumour：
Embodiment 10
Docetaxel~raspberry polysaccharide combines the inhibitory action grown to melanin tumour b16 F10 mouse xenograft tumors
Take the logarithm the melanoma cells B16F10 in growth period, is aseptically prepared into 5 × 107/ ml cell suspensions,
C57BL/6 right side of mice armpits are inoculated in 0.1ml subcutaneous.Vernier caliper measurement mice-transplanted tumor diameter is used after 5 days, tumour is treated
Grow to 60~90mm3Animal is grouped at random afterwards.Use the method in measurement knurl footpath, the antitumor effect of dynamic observation subject medicine
Really.The pendulous frequency of diameter of tumor is every 2 days 1 time, and measurement every time also needs to weigh mouse weight simultaneously.Packet and administering mode are as follows：
Docetaxel group is using being subcutaneously injected, and 10mg/kg is administered once for every three days.The low middle high administering drug combinations of docetaxel~raspberry polysaccharide
Group, docetaxel is using being subcutaneously injected, and 10mg/kg is administered once, raspberry polysaccharide uses gavage, and dosage is respectively for every three days
100mg/kg, 200mg/kg, 400mg/kg, are administered once daily.Negative control group injects normal saline, 1 time a day.It is swollen
Knurl volume calculation formula：TV=0.52 × a × b2, wherein a, b represent length and width respectively.Calculated and swollen relatively according to the result of measurement
Knurl volume.The evaluation index of antitumor activity is Relative tumor proliferation rate T/C (%), calculation formula：T/C (%)=TRTV/CRTV
× 100%, TRTV：Treatment group RTV；CRTV：Negative control group RTV.
The suppression that 10. docetaxels of table~raspberry polysaccharide drug combination grows to C57BL/6 mouse xenograft tumors is made
With
As a result：It is shown in Table 10 and Figure 10, tumour inhibiting rate of the docetaxel 10mg/kg groups to melanin tumour b16 F10 mice-transplanted tumors
It is respectively 40.95% to the tumour inhibiting rate of melanin tumour b16 F10 mice-transplanted tumors for 66.49% administering drug combinations group, 70.90% He
74.30%.The inhibition of melanoma is used alone higher than docetaxel when being used in combination.And docetaxel toxicity compared with
Greatly, the weight of animals declines substantially, and animal has death in experimentation.After both are used in combination, experimental animal body weight is without under substantially
Drop, and do not occur animal dead in experimentation.
Therefore, melanin tumour b16 F10 mice-transplanted tumors growth inhibition test result shows, compared with negative control group, respectively
Growth inhibitory action (* P ＜ 0.05, * * P ＜ with conspicuousness of the individual administration group group to melanin tumour b16 F10 mice-transplanted tumors
0.01).Growth of the low middle high administering drug combinations group of docetaxel~raspberry polysaccharide to melanin tumour b16 F10 mice-transplanted tumors has
The inhibitory action of conspicuousness, and the inhibition of the high administering drug combinations group of docetaxel~raspberry polysaccharide individually uses higher than docetaxel
Medicine group (ΔP ＜ 0.05).Illustrate that raspberry polysaccharide can strengthen the anti-tumor capacity of docetaxel.In addition, compared with docetaxel, tree
Certain kind of berries polysaccharide has no significant effect to the body weight of experimental animal, has no obvious toxicity.
Embodiment 11
Cis-platinum~raspberry polysaccharide is combined to human lung cancer H460 nude mouse xenograft knurl growth inhibition tests
Take the logarithm the tumour cell in growth period, be aseptically prepared into 5 × 107/ ml cell suspensions, are connect with 0.1ml
Plant the armpit on the right side of nude mice subcutaneous.With vernier caliper measurement transplanted tumor in nude mice diameter, treat tumour growth to 100~200mm3After move
Thing is grouped at random.Use the method in measurement knurl footpath, dynamic observation antitumous effect.The pendulous frequency of diameter of tumor is every 2 days 1
Secondary, measurement every time also needs to weigh mouse weight simultaneously.Administering mode is as follows：Negative control group injects normal saline, 1 time a day；
Cis-platinum group 10mg/kg, weekly administration 1 time；The low middle high administering drug combinations group of cis-platinum~raspberry polysaccharide, cis-platinum, which is used, to be subcutaneously injected,
10mg/kg, weekly administration 1 time, raspberry polysaccharide uses gavage, and dosage is respectively 100mg/kg, 200mg/kg, 400mg/
Kg, is administered once daily.Gross tumor volume calculation formula：
TV=0.52 × a × b2
Wherein a, b represent length and width respectively.Relative tumour volume is calculated according to the result of measurement.The evaluation of antitumor activity
Index is Relative tumor proliferation rate T/C (%), and calculation formula is as follows：
T/C (%)=TRTV/CRTV× 100%
TRTV：Treatment group RTV；CRTV：Negative control group RTV
The inhibitory action that 11. cis-platinums of table~raspberry polysaccharide drug combination grows to human lung cancer H460 nude mouse xenograft tumors
As a result：11 and Figure 11 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human lung cancer H460 transplanted tumor in nude mice
61.49%；Cis-platinum~raspberry polysaccharide combines basic, normal, high dosage group
40.95%, 65.90%, 72.30%.Wherein, the inhibition of the high administering drug combinations group of cis-platinum~raspberry polysaccharide is independent higher than cis-platinum
Medication group.In addition, toxicity of cisplatin is larger, the weight of animals declines substantially, and animal has death in experimentation.Both are used in combination
Afterwards, experimental animal body weight is without being decreased obviously, and does not occur animal dead in experimentation.
Therefore, human lung cancer H460 transplanted tumor in nude mice growth inhibition test results are shown, compared with negative control group, cis-platinum
Growth of 10mg/kg groups, cis-platinum~the raspberry polysaccharide senior middle school administering drug combinations group to human lung cancer H460 transplantable tumors has pole conspicuousness
Inhibitory action (* P ＜ 0.05, * * P ＜ 0.01).Compared with positive control cis-platinum, the high administering drug combinations group pair of cis-platinum~raspberry polysaccharide
H460 transplantable tumors growth with conspicuousness inhibitory action (ΔP ＜ 0.05), and the body weight of experimental animal has no significant effect, not
See obvious toxicity.
Embodiment 12
Taxol~raspberry polysaccharide combines the suppression experiment to the growth of the nude mouse xenograft tumor of human liver cancer Bel~7402
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injects normal saline, daily 1
It is secondary；Taxol group 10mg/kg, weekly administration 1 time；The low middle high administering drug combinations group of taxol~raspberry polysaccharide, taxol uses skin
Lower injection, 10mg/kg, weekly administration 1 time, raspberry polysaccharide uses gavage, and dosage is respectively 100mg/kg, 200mg/kg,
400mg/kg, is administered once daily.
Inhibitory action of 12. taxols of the table~raspberry polysaccharide to the growth of the nude mouse xenograft tumor of human liver cancer Bel~7402
As a result：It is shown in Table 12 and Figure 12, tumour inhibiting rate of the taxol 10mg/kg groups to the transplanted tumor in nude mice of human liver cancer Bel~7402
For 68.55%；Taxol~raspberry polysaccharide combines tumor suppression of the basic, normal, high dosage group to the transplanted tumor in nude mice of human liver cancer Bel~7402
Rate is respectively up to 45.31%, 72.47%, 76.47%.Wherein, the inhibition of taxol~raspberry polysaccharide senior middle school administering drug combinations group
Higher than taxol independent medication group (ΔP ＜ 0.05).And taxol toxicity is larger, the weight of animals declines substantially, is moved in experimentation
Thing has death.After both are used in combination, experimental animal body weight does not occur animal dead without being decreased obviously in experimentation.
Therefore, taxol~raspberry polysaccharide shows the transplanted tumor in nude mice growth inhibition test result of human liver cancer Bel~7402,
Compared with negative control group, taxol 10mg/kg groups, taxol~raspberry polysaccharide senior middle school administering drug combinations group to human liver cancer Bel~
The growth of 7402 transplantable tumors has the inhibitory action (* P ＜ 0.05, * * P ＜ 0.01) of pole conspicuousness.With positive controls Japanese yew alcohol phase
Than taxol~raspberry polysaccharide senior middle school's administering drug combinations have not significant impact to the body weight of experimental animal, have no that obvious poison is secondary anti-
Should.
Embodiment 13
Raspberry polysaccharide is to tumor-bearing mice liver, the influence of renal tissue form
After each group tumor-bearing mice anesthesia in the anti-tumor experiment of embodiment 10~12, separation liver and kidney.By liver,
Kidney is soaked in 10% formalin fixed.By the tissue fixed through ethanol dehydration, dimethylbenzene is transparent, FFPE,
Section paster, dimethylbenzene dewaxing, HE dyeing, dehydration is transparent, and sealing is after optical microphotograph Microscopic observation and takes pictures.
As shown in figure 13, the liver cell edge clear of normal mouse, cytoplasm is filled the hepatic tissue section of each group mouse, carefully
Karyon is clear, and cell arrangement is neat.By contrast, the liver cell height swelling of chemotherapeutics group mouse, subregion hepatic tissue
Focal necrosis, interstitial stove cell infiltration.And the liver cell of chemotherapeutics~raspberry polysaccharide joint group mouse has obvious recovery,
The liver cell of wherein chemotherapeutics~raspberry polysaccharide high dose joint group mouse is similar to normal mouse liver cell.It can be seen that, raspberry
The tumor-bearing mice hepatic injury that polysaccharide is caused to chemotherapeutics has certain repair.
The nephridial tissue section of each group mouse is as shown in figure 14, the glomerulus regular shape of normal mouse, Bowman's capsule gap
Small, glomerular basement membrane is complete.By contrast, there is certain degeneration and damage in the glomerulus of chemotherapeutics group mouse, specifically
Show that glomerular basement membrane is distorted, capsula glomeruli gap is substantially widened.The kidney of chemotherapeutics~raspberry polysaccharide joint group mouse is small
Ball recovers clearly, to be mainly manifested in capsula glomeruli gap and compared with chemotherapeutics group substantially diminish.It can be seen that, raspberry polysaccharide is to chemotherapeutic
The tumor-bearing mice injury of kidney that thing is caused also has certain repair.
It these results suggest that, raspberry polysaccharide can repair caused lesions of liver and kidney after chemotherapeutic drug therapy, chemotherapeutics and tree
Certain kind of berries polysaccharide is used in combination rear toxic side effect and reduced.
Embodiment 14
Influence of the raspberry polysaccharide to tumor-bearing mice index and spleen index
After the anti-tumor experiment of embodiment 10~12 terminates, by mouse anesthesia, separation tumor tissues and spleen are weighed.According to
Weighing results calculate index and spleen index.Calculation formula：Index and spleen index=spleen weight (g)/net body weight (kg), wherein, net body weight=
Body weight~knurl weight.Test obtained result to represent with mean ± SD, and carry out statistics T and examine, P ＜ 0.05 are significant difference, P
＜ 0.01 is pole significant difference (*：Compared with negative control group, # is compared with chemotherapeutics group).
As a result Figure 15 is seen.Compared with chemotherapeutics group, the spleen of the low middle high administering drug combinations group of chemotherapeutics~raspberry polysaccharide
Index is dramatically increased.
Embodiment 15
Influence of the raspberry polysaccharide to blood glucose in diabetic rats and blood fat
The present invention evaluates raspberry polyoses capsule main component using the rat diabetes model of streptozotocin (STZ) induction
The effect of the hypoglycemic and reducing blood lipid of raspberry polysaccharide.
It is as follows that model sets up process：Rat tail vein takes blood to survey blood glucose before modeling, weighs.Fasting afterwards 12 hours,
Under rat limosis state (pH4.2 0.1mol/L citrate is dissolved in by 60mg/kg concentration disposable celiac injection STZ
Buffer solution, the matching while using under the conditions of lucifuge).Tail vein takes blood to survey blood glucose after 96 hours, and blood glucose value >=16.7mmol/L's is
Think Glycemia Decline success.The successful rat of modeling is randomly divided into 5 groups, every group 10, be model group, positive control respectively
Group, raspberry polysaccharide low dose group, raspberry polysaccharide middle dosage combination raspberry polysaccharide high dose group.It is another to take 10 to make with batch healthy rat
For Normal group.The rat packet same day weighs and gastric infusion, and dosage is as follows：Normal group：Pure water；Model
Group：Pure water；Positive drug group：Glibenclamide Tablets 20mg/kg；Basic, normal, high group of dosage of raspberry polysaccharide be respectively：75mg/kg、
150mg/kg、300mg/kg.Successive administration 28 days, every 7 days measurement blood glucose are simultaneously weighed.
(1) raspberry polysaccharide causes the influence of the basic sign of diabetes rat to STZ
The raspberry polysaccharide of table 13. causes the influence of diabetes rat body weight to STZ
*P ＜ 0.05vs model groups；**P ＜ 0.01vs model groups；##P ＜ 0.01vs normal groups
Such as table 13, after modeling the body weight of each group diabetes rat be remarkably decreased compared with normal rat pole (##P ＜ 0.01vs are just
Normal group), bedding and padding meet the basic sign of diabetes compared with the humidity of normal rat.The hair color of normal rats is glossy, and bedding and padding are done
It is dry, body weight steady-state growth.The hair color of model group rats is withered, and slight depilation, bedding and padding are moist, and it is bright to increase Body weight loss with the time
It is aobvious.The hair color of each administration group rat is good compared with model group, and bedding and padding moisture conditions have mitigated compared with model group, increases flat with administration time
Though equal body weight has certain decline, downward trend is gentler than model group, wherein the body of positive controls rat last time measurement
Weight compared with model group have significant difference (*The vs model groups of p ＜ 0.05), each dosage group rat last time measurement of raspberry polysaccharide
Body weight has pole significant difference (* p ＜ 0.05vs model groups) compared with model group.Compared with positive drug glibenclamide, raspberry polysaccharide energy
More efficiently prevent from the mitigation of diabetes rat body weight.
(2) raspberry polysaccharide causes the influence of blood glucose in diabetic rats to STZ
The raspberry polysaccharide of table 14. causes the influence of blood glucose in diabetic rats to STZ
**P ＜ 0.01vs model groups；##P ＜ 0.01vs normal groups
As shown in table 14, after modeling, the blood glucose of normal rats is without significant change, and each group blood glucose in diabetic rats is more normal
Rat extremely significantly rise (##P ＜ 0.01vs normal groups) treatment after, the blood glucose of each administration group rat has extremely notable compared with model group
Property decline (**P ＜ 0.01vs model groups), and the hypoglycemic effect of raspberry polysaccharide is in dose dependent.Raspberry polysaccharide high dose group
The average blood sugar value of rat last time measurement is 17.84 ± 1.41mmol/L, is measured than positive controls rat last time
Average blood sugar 18.87 ± 2.22 it is also low.
(3) raspberry polysaccharide causes T-CHOL (TC), triglycerides (TG), high density fat in diabetes rat serum to STZ
The influence of albumen (HDL) and low-density lipoprotein (LDL)
Test the 28th day, after rats by intraperitoneal injection chloraldurate solution general anesthesia, separate arteria carotis communis and be intubated collection
Blood.3000r/min is centrifuged 10 minutes and is obtained serum sample after blood clotting.
The raspberry polysaccharide of table 15. causes the influence of diabetes rat blood fat to STZ
**P ＜ 0.01vs model groups；##P ＜ 0.01vs normal groups
As shown in Table 15, compared with normal group, the extremely significant increase of TC, TG and LDL in model group rats serum,
HDL extremely significantly declines (##P ＜ 0.01vs normal groups).After treatment, compared with model group, in each treatment group's rat blood serum
TC, TG and LDL are extremely significant to be declined, HDL extremely significantly increases (**P ＜ 0.01vs model groups).Wherein, the drop of raspberry polysaccharide
The effect of blood fat is strengthened with the increase of dosage.The lipid-lowering effect of the middle and high dosage group of raspberry polysaccharide is even better than positive control
Medicine Glibenclamide Tablets.
The antifatigue experiment of embodiment 16
The present invention evaluates the anti-fatigue ability of raspberry polysaccharide using internal pharmacodynamic experiment.Specific experiment method is as follows：
By Kunming kind male and healthy mouse (body weight：25 ± 3g, age：2~3 weeks) it is divided into 4 groups, it is that blank control group and raspberry are more respectively
The high, medium and low dosage group of sugar.The dosage of the high, medium and low dosage group of raspberry polysaccharide is respectively 200mg/kg, 100mg/kg and
50mg/kg, using gastric infusion, once a day.Blank control group gives equivalent distilled water.Successive administration 14 days.Each group mouse
Adaptability swimming instruction is proceeded by administration within the 7th day, and swimming time is incremented by 5 minutes by 10 minutes/day by day.Experiment
Last day, after being administered 30 minutes, mouse is placed in depth of water 30cm swimming trunk went swimming, water temperature is controlled at 25 DEG C or so, trip
Swimming 90 minutes.Rest extracts eyeball after 60 minutes and takes blood immediately, and the blood sample of acquirement is placed in room temperature and centrifuges (4 in a moment
DEG C, 2000 turns, 15 minutes), blood serum sample is obtained, is frozen in -80 DEG C of refrigerators standby.
(1) influence of raspberry Polysaccharides on Mice serum urea nitrogen
The influence of the raspberry Polysaccharides on Mice serum urea nitrogen of table 16.
* P ＜ 0.05, * * P ＜ 0.01vs blank control groups
Serum urea nitrogen is one of important symbol of protein in body metabolism.Motion raises serum urea nitrogen, and it is to evaluate
One very sensitive index of body ability to bear in physical load.Body is poorer to load performance, serum urea
The increase of nitrogen is more obvious.As shown in table 16, raspberry polysaccharide can significantly reduce the urea nitrogen content of mice serum, and dosage is presented
Dependence.Wherein, raspberry polysaccharide middle dose group has significant difference (* P ＜ 0.05vs blank pair compared with blank control
According to)；Raspberry polysaccharide high dose group has pole significant difference (* * P ＜ 0.01vs blank control groups) compared with blank control.
(2) in raspberry Polysaccharides on Mice serum creatine kinase influence
The influence of creatine kinase in the raspberry Polysaccharides on Mice serum of table 17.
* P ＜ 0.05, * * P ＜ 0.01vs blank control groups
Creatine kinase is closely related with energetic supersession, and it participates in the control of glycolysis, mitochondrial respiratory machine contraction of muscle and supplied
Energy.Research shows that after a large amount of motions, the creatine kinase activity in serum is significantly raised.The change of creatine kinase can be anti-in serum
Film projector body is to the adaptedness of motion, and adaptedness is higher, and serum creatine kinase activity can reduce or even recover normal level.By
Table 17 is visible, and raspberry polysaccharide can substantially reduce the content of creatine kinase in mice serum, and dose-dependence is presented.Wherein,
Raspberry polysaccharide middle dose group has significant difference (* P ＜ 0.05vs blank controls) compared with blank control；The high agent of raspberry polysaccharide
Amount group has pole significant difference (* * P ＜ 0.01vs blank control groups) compared with blank control.
(3) influence of raspberry Polysaccharides on Mice blood glucose
The influence of blood glucose in the raspberry Polysaccharides on Mice serum of table 18.
* P ＜ 0.01vs blank control groups
As shown in table 18, raspberry polysaccharide can substantially increase the content of mice serum blood glucose, and dose-dependence is presented.Its
In, the high middle dose group of raspberry polysaccharide has pole significant difference (* * P ＜ 0.01vs blank control groups) compared with blank control.
(4) in raspberry Polysaccharides on Mice serum lactic acid (LD) content influence
The influence of lactic acid in the raspberry Polysaccharides on Mice serum of table 19.
* P ＜ 0.01vs blank control groups
Lactic acid is produced by anaerobic metabolism, and it has the stability for destroying organismic internal environment, influence cardiovascular system, bone
The normal performance of musculation function, causes the generation of fatigue.Be shown in Table 19, raspberry polysaccharide it is each can pole significantly reduce mice serum
Lactic acid content (* * P ＜ 0.01vs blank control groups), and dose-dependence is presented.
(5) in raspberry Polysaccharides on Mice serum lactic dehydrogenase (LDH) content influence
The influence of lactic dehydrogenase during the raspberry Polysaccharides on Mice blood of table 20. is clear
* P ＜ 0.01vs blank control groups
Lactic acid in lactic dehydrogenase energy decomposer, the elimination that internal lactic acid is removed in time for fatigue has very positive
Effect.As shown in table 20, raspberry polysaccharide can substantially increase the content of lactic dehydrogenase in mice serum, and dose-dependant pass is presented
System.Wherein, the high middle dose group of raspberry polysaccharide has (the * * P ＜ 0.01vs blank controls of pole significant difference compared with blank control
Group).
Above-mentioned experiment shows that raspberry polysaccharide has good anti-fatigue effect.
The preparation of the lozenge of embodiment 17
1) raw material proportioning：45 parts of raspberry polysaccharide, 8 parts of microcrystalline cellulose, 1 part of citric acid, 20 parts of Icing Sugar, polyethylene glycol
60001 parts, appropriate adhesive；
2) pelletize：Each raw material crosses 80 mesh sieves respectively, mixes in proportion, and the PVP ethanol solution systems for adding appropriate 5% are soft
Material, is crossed under the conditions of the granulation of 18 mesh sieves, 60 DEG C, is dried to water content to 3% or so, is crushed, crosses 16 mesh sieve whole grains；
3) tabletting：Macrogol 6000 is added in particle, tabletting is carried out, obtains raspberry polysaccharide buccal tablet.
The preparation of the lozenge of embodiment 18
1) raw material proportioning：30 parts of raspberry polysaccharide, 8 parts of microcrystalline cellulose, 1 part of citric acid, 15 parts of Icing Sugar, Macrogol 6000
1 part, appropriate adhesive；
2) pelletize：Each raw material crosses 80 mesh sieves respectively, mixes in proportion, and the PVP ethanol solution systems for adding appropriate 5% are soft
Material, is crossed under the conditions of the granulation of 18 mesh sieves, 60 DEG C, is dried to water content to 3% or so, is crushed, crosses 16 mesh sieve whole grains；
3) tabletting：Macrogol 6000 is added in particle, tabletting is carried out, obtains raspberry polysaccharide buccal tablet.
Claims (14)
1. a kind of raspberry polysaccharide buccal tablet is preparing food, the medicine of antitumor or prevention or treatment caused by chemotherapeutic medicines toxic side effect
Purposes in product or health products, the raspberry polysaccharide buccal tablet is prepared from by the supplementary material of following weight proportion：
20~50 parts of raspberry polysaccharide, 10~30 parts of microcrystalline cellulose, 1~15 part of citric acid, 5~20 parts of Icing Sugar, lubricant 0.1~
3 parts, appropriate adhesive.
2. purposes according to claim 1, it is characterised in that：It is prepared from by the supplementary material of following weight proportion：
30~45 parts of raspberry polysaccharide, 8 parts of microcrystalline cellulose, 1 part of citric acid, 15~20 parts of Icing Sugar, 0.1~3 part of lubricant, bonding
Appropriate agent.
3. purposes according to claim 1 or 2, it is characterised in that：The lubricant is selected from Macrogol 6000 or tristearin
Sour magnesium；Further, described adhesive is selected from PVP ethanol solutions.
4. the purposes according to claims 1 to 3 any one, it is characterised in that：Its preparation method is as follows：
(1) supplementary material is taken, is mixed in proportion, suitable amount of adhesive softwood, granulation is added；
(2) lubricant is added in particle, tabletting is carried out, obtains raspberry polysaccharide buccal tablet.
5. the purposes according to claims 1 to 3 any one, it is characterised in that：In the raspberry polysaccharide, purity of polysaccharide exists
More than 50%.
6. purposes according to claim 5, it is characterised in that：Purity of polysaccharide is more than 80%.
7. purposes according to claim 6, it is characterised in that：Purity of polysaccharide is more than 85%.
8. purposes according to claim 7, it is characterised in that：Purity of polysaccharide is 85~95%w/w.
9. the purposes according to claims 1 to 3 any one, it is characterised in that：The raspberry polysaccharide uses water extract-alcohol precipitation
Method is prepared.
10. purposes according to claim 9, it is characterised in that：The water extraction and alcohol precipitation method concrete operations are as follows：
Take after dry raspberry, petroleum ether or ether defatting, extracted with 75~95%v/v of concentration ethanol, filtering, filter residue, which adds water, to be carried
Take, after water intaking extract concentration, plus ethanol to alcohol content reaches 75%~85%, cools down, stands, it is that raspberry is more to take solid content
Sugar.
11. purposes according to claim 9, it is characterised in that：Remove seed or non-remove seed before raspberry is extracted.
12. the purposes according to claims 1 to 3 any one, it is characterised in that：The raspberry is rose family rubus
The fruit of plant.
13. purposes according to claim 12, it is characterised in that：The raspberry is selected from raspberry or black raspberry.
14. purposes according to claim 1, it is characterised in that：
The tumour is melanoma, nasopharyngeal carcinoma, liver cancer, lung cancer, glioma, thyroid cancer, cancer of pancreas, the cancer of the esophagus, colon
Cancer, oophoroma or prostate cancer；The chemotherapeutics is selected from taxol, cis-platinum or/and docetaxel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510169861.7A CN104800174B (en) | 2015-04-04 | 2015-04-04 | A kind of raspberry polysaccharide buccal tablet and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510169861.7A CN104800174B (en) | 2015-04-04 | 2015-04-04 | A kind of raspberry polysaccharide buccal tablet and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104800174A CN104800174A (en) | 2015-07-29 |
| CN104800174B true CN104800174B (en) | 2017-10-13 |
Family
ID=53685674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510169861.7A Active CN104800174B (en) | 2015-04-04 | 2015-04-04 | A kind of raspberry polysaccharide buccal tablet and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104800174B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107080257A (en) * | 2017-06-20 | 2017-08-22 | 哈尔滨工业大学 | A kind of preparation of liver protecting raspberry lozenge |
| CN108477366A (en) * | 2018-01-24 | 2018-09-04 | 中科果悦湖州生物科技开发有限公司 | A kind of complex polysaccharide lozenge with anti-oxidation efficacy |
| CN108378369A (en) * | 2018-01-24 | 2018-08-10 | 中科果悦湖州生物科技开发有限公司 | A kind of complex polysaccharide effervescent tablet of anti-oxidation efficacy |
| CN108576829A (en) * | 2018-05-09 | 2018-09-28 | 哈尔滨工业大学 | One kind fight flood and relieve victims personnel it is special it is antifatigue stress chewable tablets and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731668A (en) * | 2011-04-12 | 2012-10-17 | 谭智丽 | Method for extracting and purifying Codonopsis pilosula polysaccharide |
-
2015
- 2015-04-04 CN CN201510169861.7A patent/CN104800174B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731668A (en) * | 2011-04-12 | 2012-10-17 | 谭智丽 | Method for extracting and purifying Codonopsis pilosula polysaccharide |
Non-Patent Citations (2)
| Title |
|---|
| 覆盆子多糖提取、结构分析及自由基清除作用研究;刘明学等;《食品科技》;20091231;第34卷(第7期);第163-167页 * |
| 覆盆子多糖的提取及其产品开发;李桂兰;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20150215;B016-112，第42-47页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104800174A (en) | 2015-07-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104873532B (en) | Application of the raspberry polysaccharide in antitumor | |
| CN104800174B (en) | A kind of raspberry polysaccharide buccal tablet and application thereof | |
| CN103652552A (en) | Functional food with effects of reducing lipid and preventing fatty liver | |
| CN113546097A (en) | Application of ethanol extract of cordyceps guangdongensis fruiting body in preparation of medicine for preventing obesity and hyperlipidemia | |
| CN108042627A (en) | A kind of composition for dropping hyperuricemia and its preparation method and application | |
| CN102526479A (en) | Health-care medicine formula with functions of enhancing immunity and lowering blood sugar | |
| CN103300358A (en) | Health care product capable of improving immunity | |
| CN104857018B (en) | Raspberry polysaccharide prepares the purposes of antineoplastic combination medication with chemotherapeutics | |
| CN104758270B (en) | A kind of raspberry polysaccharide effervescence tablet and application thereof | |
| CN105664140A (en) | Glycopeptide composition as well as preparation method and application thereof | |
| CN104193845B (en) | A kind of Nostoc commune antitumor polysaccharide and its preparation method and application | |
| CN104825387B (en) | A kind of raspberry polyoses oral liquid and application thereof | |
| CN105054223B (en) | A kind of collecting method of immature exocarp of Juglans mandshurica Maxim and the processing method of immature exocarp of Juglans mandshurica Maxim | |
| CN102188557A (en) | Application of Juncus effuses L. extract in preparing anti-tumor or angiogenesis-inhibiting medicament, health food or cosmetic | |
| CN104758272B (en) | A kind of raspberry polyoses capsule and application thereof | |
| CN104758309A (en) | Application of strawberry polysaccharide in reduction of blood sugar | |
| CN107519327A (en) | A kind of Phellinus Chinese medicine composition and its extracting method and the application in antineoplastic is prepared | |
| CN101829174A (en) | Application of erigeron breviscapus extract in preparing anti-tumor or angiogenesis-suppressing medicaments, health-care food or cosmetics | |
| CN105434575A (en) | Application of Eseye and extract thereof in preparation of medicines or health food for protecting liver | |
| CN104971230A (en) | Composition for boosting immunity | |
| CN104127545B (en) | New application of murraya tetramera huang and extract thereof in preparation of medicines | |
| CN104840747B (en) | Chinese medicine composition with antithyroid cancer activity and its preparation method and application | |
| CN104223066A (en) | Traditional Chinese medicine composition for improving sleeping | |
| CN104415057A (en) | Application of American cockroach lipid in tumor prevention | |
| CN112641809B (en) | Application of wood frog seed and its extract in preparing weight-reducing medicine and food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |