CN104825387B - A kind of raspberry polyoses oral liquid and application thereof - Google Patents
A kind of raspberry polyoses oral liquid and application thereof Download PDFInfo
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Abstract
The present invention provides the present invention provides a kind of raspberry polyoses oral liquids, it is prepared by the supplementary material of following weight proportion：Raspberry polysaccharide 15%~35%, remaining is distilled water.The present invention also provides the purposes of the oral liquid.Present invention research is found, raspberry polysaccharide has good active anticancer, can effectively reduce the toxic side effect of chemotherapeutics, and also have hypoglycemic and anti-fatigue effect, therefore, the present invention has good prospect of the application and social effect using the oral liquid that raspberry polysaccharide is prepared as active constituent.
Description
Technical field
The invention belongs to medical sci-tech fields, and in particular to a kind of raspberry polyoses oral liquid and preparation method thereof further relates to
The purposes of raspberry polyoses oral liquid.
Background technology
Malignant tumour is still the major disease of serious threat human life.Nearest statistics shows that China is new every year
It was found that about 20,000,000 people of cancer patient, wherein 1,500,000 people die of cancer.The death toll of cancer accounts for the 1/5 of total death toll.
Raspberry (Rubus idaeus L) also known as Rubus corchorifolius, raspberry, raspberry etc. belong to rose family rubus.Raspberry master
Northern Hemisphere temperate zone and frigid zone are distributed in, fruit sweet slightly sour to starch small aggregate fruit more.Raspberry contains polysaccharide, organic acid, Huang
Ketone, anthocyanidin, a variety of active ingredients such as polyphenol, vitamin, mineral element, volatile oil, tannin, have high nutritive value and
Medical care effect is known as " gold fruit ".At present, the raspberry product on domestic market is still based on foodstuff, such as with
Fruit juice, jam, the fruit wine of raspberry for primary raw material etc..And raspberry active ingredient is extracted, and these active ingredients are applied to protect
Strong product or even the example in medical sci-tech field are considerably less.
The report of gastric cancer can effectively be inhibited by having patent to disclose Bramble gallogen class extract at present.It is however, right at present
Raspberry polysaccharide is but without research in this respect.
Invention content
The invention reside in a kind of raspberry polyoses oral liquid is provided, another object of the present invention is to provide the use of the oral liquid
On the way.
Specifically, the present invention provides a kind of raspberry polyoses oral liquid, be prepared by the supplementary material of following weight proportion and
Into：
Raspberry polysaccharide 15%~35%, remaining is distilled water.
It is prepared by the supplementary material of following weight proportion：Raspberry polysaccharide 17%~25%, remaining is distilled water.
Wherein, oral liquor is as follows：
Raspberry polysaccharide is added in heating in distilled water makes its dissolving, is uniformly mixed, filling after cooling, sterilizes to get oral
Liquid.
Wherein, in the raspberry polysaccharide, purity of polysaccharide is more than 50%；Further, purity of polysaccharide is more than 80%；More
Further, purity of polysaccharide is more than 85%；Preferably, purity of polysaccharide is 85~95%w/w.
Wherein, the raspberry polysaccharide is prepared using water extraction and alcohol precipitation method；Further, the water extraction and alcohol precipitation method is specifically grasped
Make as follows：
It takes dry raspberry, after petroleum ether or ether defatting, is extracted with 75~95%v/v of concentration ethyl alcohol, filtering, filter residue adds water
Extraction after water intaking extract concentration, adds ethyl alcohol to alcohol content to reach 75%~85%, and cooling is stood, and it is that raspberry is more to take solid content
Sugar.
Wherein, remove seed or non-remove seed before raspberry extraction.
Wherein, the raspberry is the fruit of rose family rubus plant；Further, the raspberry be selected from raspberry or
Black raspberry.
The present invention also provides above-mentioned raspberry polyoses oral liquids to prepare antitumor or prevention or treatment chemotherapeutics institute
Purposes in the food of intoxicating side effect, drug or health products.
Further, the tumour is melanoma, nasopharyngeal carcinoma, liver cancer, lung cancer, glioma, thyroid cancer, pancreas
Cancer, the cancer of the esophagus, colon cancer, oophoroma or prostate cancer.
In the present invention, the toxic side effect is organ damage, and in the specific embodiment of the invention, the organ is liver, spleen
Or kidney.
Wherein, the chemotherapeutics is selected from taxol, cis-platinum or/and docetaxel.
Wherein, the chemotherapeutics uses injection type.Wherein, the injection type can be selected from hypodermic injection, muscle
Usual way in the various tumor therapeutic procedures such as injection or intravenous injection.
The present invention also provides above-mentioned raspberry polyoses oral liquids to prepare hypoglycemic or antifatigue drug, health products or food
Purposes in product.
Further, the drug, health products or food are prevention or drug, the health care for the treatment of I types or type-2 diabetes mellitus
Product or food.
Further, the drug, health products or food be improve the drug of dyslipidemia in diabetes, health products or
Food.
Further, the drug, health products or food are to reduce diabetic TC, TG and LDL level, are improved
Drug, health products or the food of HDL levels.
Further, the drug, health products or food are to reduce serum urea nitrogen, lactic acid, creatine kinase level, are improved
Blood glucose, the drug of lactate dehydrogenase levels, health products or food.
The present invention utilizes the internal antitumous effect of mice with tumor model evaluation raspberry polysaccharide, finally found that raspberry polysaccharide to rising
Tumour derived from the skin of people, incidence, brain, thyroid gland, pancreas, oesophagus, colon or rectum, ovary, prostate has well
Inhibiting effect, and act on, toxic side effect smaller close with chemotherapeutics, for treatment or prevention tumour provide new choosing
It selects.
Human body can generate toxic side effect, such as liver, kidney or/and splenic injury really after chemotherapeutics is used, this
Invention using raspberry polysaccharide the study found that can effectively reduce the toxic side effects such as the above-mentioned organ damage that chemotherapeutics brings, very
The antitumaous effect of chemotherapeutics can extremely be enhanced, the clinical application for cancer patient provides new selection.
To sum up, the present invention is the study found that raspberry polysaccharide can effectively reduce chemotherapeutics with good active anticancer
Toxic side effect, and also there is hypoglycemic and anti-fatigue effect, therefore, the mouth that the present invention is prepared using raspberry polysaccharide as active constituent
Liquid is taken with good prospect of the application and social effect.
Description of the drawings
1 raspberry polysaccharide of attached drawing is to melanin tumour b16 F10 mouse transplanting tumor growth inhibition effects；
2 raspberry polysaccharide of attached drawing is to human nasopharyngeal carcinoma CNE nude mouse xenograft tumor growth inhibition effects；
3 raspberry polysaccharide of attached drawing is to human glioma U87 nude mouse xenograft tumor growth inhibition effects；
4 raspberry polysaccharide of attached drawing is to human thyroid carcinomas SW-579 nude mouse xenograft tumor growth inhibition effects；
5 raspberry polysaccharide of attached drawing is to human pancreas cancer SW-1990 nude mouse xenograft tumor growth inhibition effects；
6 raspberry polysaccharide of attached drawing is to human esophagus cancer Ec109 nude mouse xenograft tumor growth inhibition effects；
7 raspberry polysaccharide of attached drawing is to human colon carcinoma HT-29 nude mouse xenograft tumor growth inhibition effects；
8 raspberry polysaccharide of attached drawing is to human ovarian cancer SK-OV-3 nude mouse xenograft tumor growth inhibition effects；
9 raspberry polysaccharide of attached drawing is to human prostata cancer DU-145 nude mouse xenograft tumor growth inhibition effects.
10 docetaxels of attached drawing~raspberry polysaccharide is to melanin tumour b16 F10 mouse transplanting tumor growth inhibition effects；
11 cis-platinums of attached drawing~raspberry polysaccharide is to human lung cancer H460 nude mouse xenograft knurl growth inhibition effects；
12 taxols of attached drawing~raspberry polysaccharide makees the inhibition of the nude mouse xenograft tumor of human liver cancer Bel~7402 growth
With；
13 chemotherapeutics of attached drawing~raspberry polysaccharide combines the influence to tumor-bearing mice liver organization form；
14 chemotherapeutics of attached drawing~raspberry polysaccharide combines the influence to tumor-bearing mice renal tissue form；
15 chemotherapeutics of attached drawing~raspberry polysaccharide combines the influence to C57BL/6 mouse spleen indexes.
Specific embodiment
The raspberry polysaccharide used in the specific embodiment of the invention is prepared into using conventional Polyose extraction, way of purification
It arrives, concrete operations are as follows in the present invention：
It is prepared by raspberry polysaccharide：Dry raspberry is taken, after crushing remove seed, adds 5 times of petroleum ethers (60~90 DEG C of boiling range) degreasing, eliminates stone
75~95%v/v ethyl alcohol is added in after oily ether in 60 DEG C of reflux, the filter residue after filtering is added into 10 times of pure water ultrasonic extraction (power
60W, temperature：80 DEG C, processing time：100min), centrifuging and taking supernatant, filter residue are extracted twice again by old terms, and merging filtrate subtracts
Pressure is concentrated into the 1/4 of original volume, adds in 95%v/v ethyl alcohol, and solution alcohol content is made to be stood up to 75% in 4 degrees Celsius for 24 hours, filtering
Filter residue is collected, filter residue dries to obtain raspberry polysaccharide.
After measured, the raspberry polysaccharide yield that prepared by the present invention is 10.4%~11.6%, detects to obtain through Phenol-sulphate acid method
Purity of polysaccharide is 90.8%~91.2%.
In various embodiments of the present invention, raspberry polysaccharide dosage is in terms of polysaccharide.
Embodiment 1
The inhibiting effect that raspberry polysaccharide grows melanin tumour b16 F10 mouse xenograft tumors
Take the logarithm the melanoma cells B16F10 in growth period, is aseptically prepared into 5 × 107/ ml cell suspensions,
It is subcutaneous that C57BL/6 right side of mice armpits are inoculated in 0.1ml.With vernier caliper measurement mice-transplanted tumor diameter after 5 days, tumour is treated
Grow to 60-90mm3Animal is grouped at random afterwards.Using the method for measuring knurl footpath, dynamic observation is tested the antitumous effect of drug.
The pendulous frequency of diameter of tumor is every 2 days 1 time, and measure also needs to weigh mouse weight simultaneously every time.Grouping and administering mode are as follows：It is negative
Control group injects normal saline, 1 time a day.Positive controls (docetaxel), which use, to be subcutaneously injected, 10mg/kg, and every three
It is administered once.The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg, 200mg/kg, 400mg/kg, using gastric infusion,
1 time a day.
Gross tumor volume calculation formula：TV=0.52 × a × b2, wherein a, b represent length and width respectively.According to the result meter of measurement
Calculate relative tumour volume.The evaluation index of antitumor activity be Relative tumor proliferation rate T/C (%), calculation formula：T/C (%)
=TRTV/CRTV× 100%, TRTV：Treatment group RTV：CRTV：Negative control group RTV.
The inhibiting effect that 1. raspberry polysaccharide of table grows melanin tumour b16 F10 mouse xenograft tumors
As a result：It is shown in Table 1 and Fig. 1, suppressions of the positive control docetaxel 10mg/kg to melanin tumour b16 F10 mice-transplanted tumors
Ratio of outflow is 66.49%.The low middle high dose group of raspberry polysaccharide is respectively to the tumour inhibiting rate of melanin tumour b16 F10 mice-transplanted tumors
7.56%, 24.32 and 60.95%.Compared with negative control group, docetaxel and raspberry high dose are small to melanin tumour b16 F10
Inhibiting effect (* P ＜ 0.05, * * P ＜ 0.01) of the growth with conspicuousness of mouse transplantable tumor.But docetaxel toxicity is larger, moves
Object weight loss is apparent, and animal has death in experimentation.Raspberry polysaccharide has no significant effect the weight of experimental animal, has no bright
Aobvious toxicity.
Embodiment 2
Raspberry polysaccharide is to human nasopharyngeal carcinoma CNE nude mouse xenograft tumor growth inhibition tests
It takes the logarithm the tumour cell in growth period, is aseptically prepared into 5 × 107/ ml cell suspensions, are connect with 0.1ml
Kind armpit on the right side of nude mice is subcutaneous.With vernier caliper measurement transplanted tumor in nude mice diameter, treat tumour growth to 100-200mm3After move
Object is grouped at random.Use the method for measuring knurl footpath, the antitumous effect of dynamic observation raspberry polysaccharide.The pendulous frequency of diameter of tumor
It it is every 2 days 1 time, measure also needs to weigh mouse weight simultaneously every time.
Administering mode is as follows：Negative control group injects normal saline, 1 time a day；Cis-platinum group 10mg/kg, using skin
Lower injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg, 200mg/kg, 400mg/kg, is used
Gastric infusion, 1 time a day.Gross tumor volume is calculated with embodiment 1
The inhibiting effect that 2. raspberry polysaccharide of table grows human nasopharyngeal carcinoma CNE nude mouse xenograft tumors
As a result：2 and Fig. 2 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human nasopharyngeal carcinoma CNE transplanted tumor in nude mice
70.38%；The basic, normal, high dosage group of raspberry polysaccharide is respectively 39.77% to the tumour inhibiting rate of human nasopharyngeal carcinoma CNE transplanted tumor in nude mice,
52.36%, 65.18%.But toxicity of cisplatin is larger, and the weight of animals declines significantly, and animal has death in experimentation.And raspberry is more
Sugar influences experimental animal weight without conspicuousness.
Therefore, raspberry polysaccharide to human nasopharyngeal carcinoma CNE transplanted tumor in nude mice growth inhibition test the result shows that, with negative control group
It compares, raspberry polysaccharide high dose group is to inhibiting effect (* * P ＜ of the growth with pole conspicuousness of human nasopharyngeal carcinoma CNE transplantable tumors
0.07), raspberry polysaccharide middle dose group is to inhibiting effect (* P ＜ of the growth with conspicuousness of human nasopharyngeal carcinoma CNE transplantable tumors
0.05).Compared with positive control cis-platinum, raspberry polysaccharide has no significant effect the weight of experimental animal, has no that apparent poison is secondary anti-
It should.
Embodiment 3
Raspberry polysaccharide tests the inhibition that human glioma U87 nude mouse xenograft tumors are grown
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injection normal saline, daily 1
It is secondary；Taxol group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/
Kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibiting effect that 3. raspberry polysaccharide of table grows human glioma U87 nude mouse xenograft tumors
As a result：3 and Fig. 3 are shown in Table, taxol 10mg/kg groups are to the tumour inhibiting rate of human glioma U87 transplanted tumor in nude mice
73.11%；The high, medium and low dosage group of raspberry polysaccharide to the tumour inhibiting rate of human glioma U87 transplanted tumor in nude mice respectively up to 78.19%,
76.21%, 69.73%.But taxol toxicity is larger, and the weight of animals declines, and animal has death in experimentation.And raspberry polysaccharide
There is no conspicuousness influence to nude mice weight.
Therefore, raspberry polysaccharide to human glioma U87 transplanted tumor in nude mice growth inhibition test the result shows that, with negative control
Group is compared, and the high, medium and low dosage group of raspberry polysaccharide is respectively provided with the growth of human glioma U87 transplantable tumors the inhibition of pole conspicuousness
It acts on (* * P ＜ 0.01).Compared with positive controls taxol, raspberry polysaccharide has not significant impact the weight of experimental animal,
Have no apparent toxicity.
Embodiment 4
Raspberry polysaccharide human thyroid carcinomas SW-579 nude mouse xenograft tumor growth inhibition tests
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Dosage regimen is as follows：Negative control group injects equivalent
Physiological saline, 1 time a day；5 FU 5 fluorouracil group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle height of raspberry polysaccharide
Dosage group is respectively 100mg/kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibiting effect that 4. raspberry polysaccharide of table grows human thyroid carcinomas SW-579 nude mouse xenograft tumors
As a result：4 and Fig. 4 are shown in Table, 5-Fu (5 one fluorouracil) 10mg/kg groups are to human thyroid carcinomas SW-579 nude mice models
The tumour inhibiting rate of knurl is 69.26%；The high, medium and low dosage group of raspberry polysaccharide is to the tumor suppression of human thyroid carcinomas SW-579 transplanted tumor in nude mice
Rate is respectively up to 70.81%, 62.35%, 57.98%.But 5-Fu toxicity is larger, and the weight of animals declines, and there is animal in experimentation
It is dead.And raspberry polysaccharide does not have conspicuousness influence to nude mice weight.
Therefore, raspberry polysaccharide to human thyroid carcinomas SW-579 transplanted tumor in nude mice growth inhibition test the result shows that, with feminine gender
Control group is compared, and the high, medium and low dosage group group of raspberry polysaccharide extremely shows being respectively provided with for growth of human thyroid carcinomas SW-579 transplantable tumors
The inhibiting effect (* * P ＜ 0.01) of work property.Compared with positive controls 5-Fu, raspberry polysaccharide is not bright to the weight of experimental animal
Development is rung, and has no apparent toxicity.
Embodiment 5
Raspberry polysaccharide is to human pancreas cancer SW-1990 nude mouse xenograft tumor growth inhibition tests
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injection normal saline, daily 1
It is secondary；5 FU 5 fluorouracil group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively
100mg/kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibiting effect that 5. raspberry polysaccharide of table grows human pancreas cancer SW-1990 nude mouse xenograft tumors
As a result：5 and Fig. 5 are shown in Table, 5-Fu 10mg/kg groups are to the tumour inhibiting rate of human pancreas cancer SW-1990 transplanted tumor in nude mice
65.26%；The high, medium and low dosage group of raspberry polysaccharide reaches the tumour inhibiting rate of human pancreas cancer SW-1990 transplanted tumor in nude mice respectively
69.08%, 53.82%, 45.31%.But 5-Fu toxicity is larger, and the weight of animals declines, and animal has death in experimentation.And it sets
Certain kind of berries polysaccharide does not have conspicuousness influence to nude mice weight.
Therefore, raspberry polysaccharide to human pancreas cancer SW-1990 transplanted tumor in nude mice growth inhibition test the result shows that, it is right with feminine gender
It is compared according to group, raspberry polysaccharide high dose group has the growth of human pancreas cancer SW-1990 transplantable tumors inhibiting effect (the * * of pole conspicuousness
P ＜ 0.01), significant inhibiting effect (the * P ＜ of growth of the raspberry polysaccharide middle dose group to human pancreas cancer SW-1990 transplantable tumors
0.05).Compared with positive controls 5-Fu, raspberry polysaccharide has not significant impact the weight of experimental animal, has no apparent poison
Side reaction.
Embodiment 6
Raspberry polysaccharide is to human esophagus cancer Ec109 nude mouse xenograft tumor growth inhibition tests
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injection normal saline, daily 1
It is secondary；Taxol group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/
Kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibiting effect that 6. raspberry polysaccharide of table grows human esophagus cancer Ec109 nude mouse xenograft tumors
As a result：6 and Fig. 6 are shown in Table, taxol 10mg/kg groups are to the tumour inhibiting rate of human esophagus cancer Ec109 transplanted tumor in nude mice
69.76%；The high, medium and low dosage group of raspberry polysaccharide to the inhibitory rate 67.55% of human esophagus cancer Ec109 transplanted tumor in nude mice,
50.03%, 40.21%.But taxol toxicity is larger, and the weight of animals declines significantly, has death during experiment.And raspberry polysaccharide
There is no conspicuousness influence to nude mice weight.
Therefore, raspberry polysaccharide to human esophagus cancer Ec109 transplanted tumor in nude mice growth inhibition test the result shows that, with negative control
Group is compared, and raspberry polysaccharide high dose group has the growth of human esophagus cancer Ec109 transplantable tumors inhibiting effect (the * * P ＜ of pole conspicuousness
0.01), significant inhibiting effect (the * P ＜ of growth of the raspberry polysaccharide middle dose group to human esophagus cancer Ec109 transplantable tumors
0.05).Compared with positive controls taxol, raspberry polysaccharide has not significant impact the weight of experimental animal, has no apparent
Toxicity.
Embodiment 7
Raspberry polysaccharide tests the inhibition that human colon carcinoma HT-29 nude mouse xenograft tumors are grown
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injection normal saline, daily 1
It is secondary；Taxol group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/
Kg, 200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibiting effect that 7. raspberry polysaccharide of table grows human colon carcinoma HT-29 nude mouse xenograft tumors
As a result：7 and Fig. 7 are shown in Table, taxol 10mg/kg groups are to the tumour inhibiting rate of human colon carcinoma HT-29 transplanted tumor in nude mice
68.23%；The high, medium and low dosage group of raspberry polysaccharide is respectively 62.88% to the tumour inhibiting rate of human colon carcinoma HT-29 transplanted tumor in nude mice,
53.13%, 45.33%.But taxol toxicity is larger, and the weight of animals declines significantly, has death during experiment.And raspberry polysaccharide
There is no conspicuousness influence to nude mice weight.
Therefore, raspberry polysaccharide to human colon carcinoma HT-29 transplanted tumor in nude mice growth inhibition test the result shows that, with negative control
Group is compared, and raspberry polysaccharide high dose group has the growth of human colon carcinoma HT-29 transplantable tumors inhibiting effect (the * * P ＜ of pole conspicuousness
0.01), significant inhibiting effect (the * P ＜ of growth of the raspberry polysaccharide middle dose group to human colon carcinoma HT-29 transplantable tumors
0.05).Compared with positive controls taxol, raspberry polysaccharide has not significant impact the weight of experimental animal, has no apparent
Toxicity.
Embodiment 8
The inhibition experiment of raspberry polysaccharide human ovarian cancer SK-OV-3 nude mouse xenograft tumors growth
Specific embodiment is with reference to embodiment 2.Administering mode is as follows：Negative control group injection normal saline, daily 1
It is secondary；Cis-platinum group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg,
200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibiting effect that 8. raspberry polysaccharide of table grows human ovarian cancer SK-OV-3 nude mouse xenograft tumors
As a result：8 and Fig. 8 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human ovarian cancer SK-OV-3 transplanted tumor in nude mice
The 68.24% high, medium and low dosage group of raspberry polysaccharide is respectively to the tumour inhibiting rate of human ovarian cancer SK-OV-3 transplanted tumor in nude mice
70.13%, 59.87%, 48.08%.But toxicity of cisplatin is larger, and the weight of animals declines significantly, has death during experiment.And it sets
Certain kind of berries polysaccharide does not have conspicuousness influence to nude mice weight.
Therefore, raspberry polysaccharide to human ovarian cancer SK-OV-3 transplanted tumor in nude mice growth inhibition test the result shows that, it is right with feminine gender
It is compared according to group, raspberry polysaccharide height, middle dose group have the growth of human ovarian cancer SK-OV-3 transplantable tumors the inhibiting effect of pole conspicuousness
(* * P ＜ 0.01), the significant inhibiting effect (* of growth of the raspberry polysaccharide low dose group to human ovarian cancer SK-OV-3 transplantable tumors
P ＜ 0.05).Compared with positive controls cis-platinum, raspberry polysaccharide has not significant impact the weight of experimental animal, has no apparent
Toxicity.
Embodiment 9
Raspberry polysaccharide tests the inhibition that human prostata cancer DU-145 nude mouse xenograft tumors are grown
Specific embodiment is with reference to embodiment 2.Administering mode is as follows：Negative control group injection normal saline, daily 1
It is secondary；Cis-platinum group 10mg/kg, using hypodermic injection, weekly administration 1 time；The low middle high dose group of raspberry polysaccharide is respectively 100mg/kg,
200mg/kg, 400mg/kg, using gastric infusion, 1 time a day.
The inhibiting effect that 9. raspberry polysaccharide of table grows human prostata cancer DU-145 nude mouse xenograft tumors
As a result：9 and Fig. 9 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human prostata cancer DU-145 transplanted tumor in nude mice
71.38%；The high, medium and low dosage group of raspberry polysaccharide is respectively to the tumour inhibiting rate of human prostata cancer DU-145 transplanted tumor in nude mice
75.31%, 55.63%, 48.27%.But toxicity of cisplatin is larger, and the weight of animals declines significantly, has death during experiment.And it sets
Certain kind of berries polysaccharide does not have conspicuousness influence to nude mice weight.
Therefore, raspberry polysaccharide to human prostata cancer DU-145 transplanted tumor in nude mice growth inhibition test the result shows that, with feminine gender
Control group is compared, and raspberry polysaccharide high dose group has the growth of human prostata cancer DU-145 transplantable tumors the inhibiting effect of pole conspicuousness
(* * P ＜ 0.01), in raspberry polysaccharide, the inhibition that growth of the low dose group to human prostata cancer DU-145 transplantable tumors is significant makees
With (* P ＜ 0.05).Compared with positive controls cis-platinum, raspberry polysaccharide influences the weight of experimental animal without conspicuousness, has no bright
Aobvious toxicity.
The present invention is in terms of tumor-inhibiting action, attenuation synergistic and immunological regulation three to the main component of raspberry polyoses oral liquid
Raspberry polysaccharide has carried out the pharmacodynamic study of adjuvant for chemotherapy of tumour：
Embodiment 10
Docetaxel~raspberry polysaccharide combines the inhibiting effect grown to melanin tumour b16 F10 mouse xenograft tumors
Take the logarithm the melanoma cells B16F10 in growth period, is aseptically prepared into 5 × 107/ ml cell suspensions,
It is subcutaneous that C57BL/6 right side of mice armpits are inoculated in 0.1ml.With vernier caliper measurement mice-transplanted tumor diameter after 5 days, tumour is treated
Grow to 60~90mm3Animal is grouped at random afterwards.Using the method for measuring knurl footpath, dynamic observation is tested the antitumor effect of drug
Fruit.The pendulous frequency of diameter of tumor is every 2 days 1 time, and measure also needs to weigh mouse weight simultaneously every time.Grouping and administering mode are as follows：
Using being subcutaneously injected, 10mg/kg is administered once docetaxel group every three days.The low middle high administering drug combinations of docetaxel~raspberry polysaccharide
Group, using being subcutaneously injected, 10mg/kg is administered once docetaxel every three days, and raspberry polysaccharide uses gavage, and dosage is respectively
100mg/kg, 200mg/kg, 400mg/kg are administered once daily.Negative control group injects normal saline, 1 time a day.It is swollen
Knurl accumulates calculation formula：TV=0.52 × a × b2, wherein a, b represent length and width respectively.It is calculated according to the result of measurement relatively swollen
Knurl is accumulated.The evaluation index of antitumor activity be Relative tumor proliferation rate T/C (%), calculation formula：T/C (%)=TRTV/CRTV
× 100%, TRTV：Treatment group RTV；CRTV：Negative control group RTV.
10. docetaxels of table~raspberry polysaccharide drug combination makees the inhibition that C57BL/6 mouse xenograft tumors are grown
With
As a result：10 and Figure 10 are shown in Table, docetaxel 10mg/kg groups are to the tumour inhibiting rate of melanin tumour b16 F10 mice-transplanted tumors
It is respectively 40.95% to the tumour inhibiting rate of melanin tumour b16 F10 mice-transplanted tumors for 66.49% administering drug combinations group, 70.90% He
74.30%.The inhibition of melanoma is used alone higher than docetaxel when being used in combination.And docetaxel toxicity compared with
Greatly, the weight of animals declines apparent, and animal has death in experimentation.After the two is used in combination, experimental animal weight is without under apparent
Drop, and do not occur animal dead in experimentation.
Therefore, melanin tumour b16 F10 mice-transplanted tumors growth inhibition test the result shows that, compared with negative control group, respectively
A administration group group is to inhibiting effect (* P ＜ 0.05, * * P ＜ of the growth with conspicuousness of melanin tumour b16 F10 mice-transplanted tumors
0.01).The low middle high administering drug combinations group of docetaxel~raspberry polysaccharide has the growth of melanin tumour b16 F10 mice-transplanted tumors
The inhibiting effect of conspicuousness, and the inhibition of the high administering drug combinations group of docetaxel~raspberry polysaccharide is individually used higher than docetaxel
Medicine group (ΔP ＜ 0.05).Illustrate that raspberry polysaccharide can enhance the anti-tumor capacity of docetaxel.In addition, compared with docetaxel, tree
Certain kind of berries polysaccharide has no significant effect the weight of experimental animal, has no apparent toxicity.
Embodiment 11
Cis-platinum~raspberry polysaccharide is combined to human lung cancer H460 nude mouse xenograft knurl growth inhibition tests
It takes the logarithm the tumour cell in growth period, is aseptically prepared into 5 × 107/ mi cell suspensions, are connect with 0.1ml
Kind armpit on the right side of nude mice is subcutaneous.With vernier caliper measurement transplanted tumor in nude mice diameter, treat tumour growth to 100~200mm3After move
Object is grouped at random.Use the method for measuring knurl footpath, dynamic observation antitumous effect.The pendulous frequency of diameter of tumor is every 2 days 1
Secondary, measure also needs to weigh mouse weight simultaneously every time.Administering mode is as follows：Negative control group injects normal saline, 1 time a day；
Cis-platinum group 10mg/kg, weekly administration 1 time；The low middle high administering drug combinations group of cis-platinum~raspberry polysaccharide, cis-platinum, which uses, to be subcutaneously injected,
10mg/kg, weekly administration 1 time, raspberry polysaccharide use gavage, and dosage is respectively 100mg/kg, 200mg/kg, 400mg/
Kg is administered once daily.Gross tumor volume calculation formula：
TV=0.52 × a × b2
Wherein a, b represent length and width respectively.Relative tumour volume is calculated according to the result of measurement.The evaluation of antitumor activity
Index is Relative tumor proliferation rate T/C (%), and calculation formula is as follows：
T/C (%)=TRTV/CRTV× 100%
TRTV：Treatment group RTV；CRTV：Negative control group RTV
The inhibiting effect that 11. cis-platinums of table~raspberry polysaccharide drug combination grows human lung cancer H460 nude mouse xenograft tumors
As a result：11 and Figure 11 are shown in Table, cis-platinum 10mg/kg groups are to the tumour inhibiting rate of human lung cancer H460 transplanted tumor in nude mice
61.49%；Cis-platinum~raspberry polysaccharide combines basic, normal, high dosage group
40.95%, 65.90%, 72.30%.Wherein, the inhibition of the high administering drug combinations group of cis-platinum~raspberry polysaccharide is independent higher than cis-platinum
Medication group.In addition, toxicity of cisplatin is larger, the weight of animals declines significantly, and animal has death in experimentation.The two is used in combination
Afterwards, experimental animal weight is without being decreased obviously, and do not occur animal dead in experimentation.
Therefore, to human lung cancer H460 transplanted tumor in nude mice growth inhibition test the result shows that, compared with negative control group, cis-platinum
10mg/kg groups, cis-platinum~raspberry polysaccharide senior middle school administering drug combinations group have pole conspicuousness to the growth of human lung cancer H460 transplantable tumors
Inhibiting effect (* P ＜ 0.05, * * P ＜ 0.01).Compared with positive control cis-platinum, the high administering drug combinations group pair of cis-platinum~raspberry polysaccharide
H460 transplantable tumors growth with conspicuousness inhibiting effect (ΔP ＜ 0.05), and the weight of experimental animal has no significant effect, not
See apparent toxicity.
Embodiment 12
Taxol~raspberry polysaccharide, which is combined, tests the inhibition of the nude mouse xenograft tumor of human liver cancer Bel~7402 growth
Specific embodiment is with reference to embodiment 2.Dosage regimen is as follows：Negative control group injection normal saline, daily 1
It is secondary；Taxol group 10mg/kg, weekly administration 1 time；The low middle high administering drug combinations group of taxol~raspberry polysaccharide, taxol use skin
Lower injection, 10mg/kg, weekly administration 1 time, raspberry polysaccharide use gavage, and dosage is respectively 100mg/kg, 200mg/kg,
400mg/kg is administered once daily.
12. taxols of table~raspberry polysaccharide is to the inhibiting effect of the nude mice xenograft tumor growths of human liver cancer Bel~7402
As a result：It is shown in Table 12 and Figure 12, taxol 10mg/kg groups are to the tumour inhibiting rate of the transplanted tumor in nude mice of human liver cancer Bel~7402
It is 68.55%；Taxol~raspberry polysaccharide combines tumor suppression of the basic, normal, high dosage group to the transplanted tumor in nude mice of human liver cancer Bel~7402
Rate is respectively up to 45.31%, 72.47%, 76.47%.Wherein, the inhibition of taxol~raspberry polysaccharide senior middle school administering drug combinations group
Higher than taxol independent medication group (ΔP ＜ 0.05).And taxol toxicity is larger, the weight of animals declines significantly, is moved in experimentation
Object has death.After the two is used in combination, experimental animal weight is without being decreased obviously, and do not occur animal dead in experimentation.
Therefore, taxol~raspberry polysaccharide to the transplanted tumor in nude mice growth inhibition test of human liver cancer Bel~7402 the result shows that,
Compared with negative control group, taxol 10mg/kg groups, taxol~raspberry polysaccharide senior middle school administering drug combinations group to human liver cancer Bel~
The growth of 7402 transplantable tumors has the inhibiting effect (* P ＜ 0.05, * * P ＜ 0.01) of pole conspicuousness.With positive controls Japanese yew alcohol phase
Than taxol~raspberry polysaccharide senior middle school's administering drug combinations have not significant impact the weight of experimental animal, have no that apparent poison is secondary anti-
It should.
Embodiment 13
Influence of the raspberry polysaccharide to tumor-bearing mice liver, renal tissue form
After each group tumor-bearing mice anesthesia in 10~12 anti-tumor experiment of embodiment, liver and kidney are detached.By liver,
Kidney is soaked in 10% formalin fixed.By the tissue fixed through ethanol dehydration, dimethylbenzene is transparent, paraffin embedding,
Patch is sliced, dimethylbenzene dewaxing, HE dyeing, dehydration is transparent, and sealing is after optical microphotograph Microscopic observation and takes pictures.
The hepatic tissue section of each group mouse is as shown in figure 13, and the liver cell edge clear of normal mouse, cytoplasm fills, carefully
Karyon is clear, and cell arrangement is neat.In contrast, the liver cell height swelling of chemotherapeutics group mouse, subregion hepatic tissue
Focal necrosis, interstitial stove cell infiltration.And the liver cell of chemotherapeutics~raspberry polysaccharide joint group mouse has apparent recovery,
The liver cell of wherein chemotherapeutics~raspberry polysaccharide high dose joint group mouse is similar to normal mouse liver cell.As it can be seen that raspberry
Polysaccharide tumor-bearing mice hepatic injury caused by chemotherapeutics has certain repair.
The nephridial tissue slice of each group mouse is as shown in figure 14, the glomerulus regular shape of normal mouse, Bowman's capsule gap
Small, glomerular basement membrane is complete.In contrast, there is certain degeneration and damage in the glomerulus of chemotherapeutics group mouse, specifically
Show that glomerular basement membrane distorts, capsula glomeruli gap is significantly widened.The kidney of chemotherapeutics~raspberry polysaccharide joint group mouse is small
Ball restores clearly, to be mainly manifested in capsula glomeruli gap and compared with chemotherapeutics group significantly become smaller.As it can be seen that raspberry polysaccharide is to chemotherapeutic
Tumor-bearing mice injury of kidney also has certain repair caused by object.
It these results suggest that, raspberry polysaccharide can repair caused lesions of liver and kidney after chemotherapeutic drug therapy, chemotherapeutics and tree
Certain kind of berries polysaccharide is used in combination rear toxic side effect and is reduced.
Embodiment 14
Influence of the raspberry polysaccharide to tumor-bearing mice index and spleen index
After 10~12 anti-tumor experiment of embodiment, by mouse anesthesia, tumor tissues and spleen are detached, is weighed.According to
Weighing results calculate index and spleen index.Calculation formula：Index and spleen index=spleen weight (g)/net weight (kg), wherein, net weight=
Weight~knurl weight.It tests obtained result to represent with mean ± SD, and carries out statistics T and examine, P ＜ 0.05 are significant difference, P
＜ 0.01 is pole significant difference (*：Compared with negative control group, # is compared with chemotherapeutics group).
The result is shown in Figure 15.Compared with chemotherapeutics group, the spleen of the low middle high administering drug combinations group of chemotherapeutics~raspberry polysaccharide
Index dramatically increases.
Embodiment 15
Influence of the raspberry polysaccharide to blood glucose in diabetic rats and blood fat
The present invention evaluates raspberry polyoses capsule main component using the rat diabetes model of streptozotocin (STZ) induction
The effect of the hypoglycemic and reducing blood lipid of raspberry polysaccharide.
Model foundation process is as follows：Rat tail vein takes blood to survey blood glucose before modeling, weighs.Fasting later 12 hours,
Under rat limosis state (citrate of the 0.1mol/L of pH4.2 is dissolved in by the concentration disposable celiac of 60mg/kg injection STZ
Buffer solution, the matching while using under the conditions of being protected from light).96 hours rear molding venous blood samplings survey blood glucose, and blood glucose value >=16.7mmol/L's is
Think Glycemia Decline success.The successful rat of modeling is randomly divided into 5 groups, every group 10, be model group, positive control respectively
Group, raspberry polysaccharide low dose group, raspberry polysaccharide middle dosage combination raspberry polysaccharide high dose group.It is another to take 10 to make with batch healthy rat
For Normal group.The rat grouping same day weighs and gastric infusion, dosage are as follows：Normal group：Pure water；Model
Group：Pure water；Positive drug group：Glibenclamide Tablets 20mg/kg；Basic, normal, high group of dosage of raspberry polysaccharide be respectively：75mg/kg、
150mg/kg、300mg/kg.Successive administration 28 days measures blood glucose for every 7 days and weighs.
(1) raspberry polysaccharide causes STZ the influence of the basic sign of diabetes rat
13. raspberry polysaccharide of table causes STZ the influence of diabetes rat body weight
*P ＜ 0.05vs model groups；**P ＜ 0.01vs model groups；##Normal group of p ＜ 0.01vs
Such as table 13, after modeling the weight of each group diabetes rat be remarkably decreased compared with normal rat pole (##P ＜ 0.01vs are just
Normal group), bedding and padding meet the basic sign of diabetes compared with the humidity of normal rat.The hair color of normal rats is glossy, and bedding and padding are done
It is dry, weight steady-state growth.The hair color of model group rats is withered, slight to lose hair or feathers, and bedding and padding are moist, and it is bright to increase weight loss at any time
It is aobvious.The hair color of each administration group rat is good compared with model group, and bedding and padding moisture conditions are mitigated compared with model group, increases with administration time flat
Though equal weight has certain decline, downward trend is gentler than model group, the body that wherein positive controls rat last time measures
Weight compared with the significant difference of model group (*P ＜ 0.05vs model groups), the body that each dosage group rat last time of raspberry polysaccharide measures
Weight compared with model group have pole significant difference (*P ＜ 0.05vs model groups).Compared with positive drug glibenclamide, raspberry polysaccharide can be more
It is effectively prevented the mitigation of diabetes rat body weight.
(2) raspberry polysaccharide causes STZ the influence of blood glucose in diabetic rats
14. raspberry polysaccharide of table causes STZ the influence of blood glucose in diabetic rats
**P ＜ 0.01vs model groups；##Normal group of p ＜ 0.01vs
As shown in table 14, after modeling, without significant change, each group blood glucose in diabetic rats is more normal for the blood glucose of normal rats
Rat extremely significantly raising (##Normal group of p ＜ 0.01vs) after treatment, the blood glucose of each administration group rat has extremely significantly compared with model group
Property decline (**P ＜ 0.01vs model groups), and the hypoglycemic effect of raspberry polysaccharide is in dose dependent.Raspberry polysaccharide high dose group
The average blood sugar value that rat last time measures is 17.8441.41mmol/L, is measured than positive controls rat last time
Average blood sugar 18.87 ± 2.22 it is also low.
(3) raspberry polysaccharide causes T-CHOL (TC), triglycerides (TG), high density fat in diabetes rat serum to STZ
The influence of albumen (HDL) and low-density lipoprotein (LDL)
It tests the 28th day, after rats by intraperitoneal injection chloraldurate solution general anesthesia, detaches arteria carotis communis and be intubated collection
Blood.3000r/min is centrifuged 10 minutes and is obtained serum sample after blood clotting.
15. raspberry polysaccharide of table causes STZ the influence of diabetes rat blood fat
**P ＜ 0.01vs model groups；##Normal group of p ＜ 0.01vs
As shown in Table 15, compared with normal group, the extremely significant increase of TC, TG and LDL in model group rats serum,
HDL extremely significantly declines (##Normal group of p ＜ 0.01vs).After treatment, compared with model group, in each treatment group's rat blood serum
TC, TG and LDL are extremely significant to be declined, HDL extremely significantly increases (**P ＜ 0.01vs model groups).Wherein, the drop of raspberry polysaccharide
The effect of blood fat is strengthened with the increase of dosage.The lipid-lowering effect of the middle and high dosage group of raspberry polysaccharide is even better than positive control
Medicine Glibenclamide Tablets.
16 antifatigue experiment of embodiment
The present invention evaluates the anti-fatigue ability of raspberry polysaccharide using internal pharmacodynamic experiment.Specific experimental method is as follows：
By Kunming kind male and healthy mouse (weight：25 ± 3g, age：2~3 weeks) it is divided into 4 groups, it is that blank control group and raspberry are more respectively
The high, medium and low dosage group of sugar.The dosage of the high, medium and low dosage group of raspberry polysaccharide is respectively 200mg/kg, 100mg/kg and
50mg/kg, using gastric infusion, once a day.Blank control group gives equivalent distilled water.Successive administration 14 days.Each group mouse
Adaptability swimming instruction is proceeded by administration within the 7th day, swimming time is by 10 minutes/day, is incremented by 5 minutes by day.Experiment
Mouse after being administered 30 minutes, is placed in the swimming trunk went swimming of depth of water 30cm by last day, and water temperature is controlled at 25 DEG C or so, trip
Swimming 90 minutes.Rest extracts eyeball and takes blood immediately after sixty minutes, and the blood sample of acquirement is being placed at room temperature for centrifugation (4 in a moment
DEG C, 2000 turns, 15 minutes), blood serum sample is obtained, is frozen in -80 DEG C of refrigerators spare.
(1) influence of raspberry Polysaccharides on Mice serum urea nitrogen
The influence of 16. raspberry Polysaccharides on Mice serum urea nitrogen of table
* P ＜ 0.05, * * P ＜ 0.01vs blank control groups
Serum urea nitrogen is the one of the important signs that of protein in body metabolism.Movement increases serum urea nitrogen, it is evaluation
One very sensitive index of body ability to bear in physical load.Body is poorer to load performance, serum urea
The increase of nitrogen is more apparent.As shown in table 16, raspberry polysaccharide can significantly reduce the urea nitrogen content of mice serum, and dosage is presented
Dependence.Wherein, raspberry polysaccharide middle dose group has significant difference (* P ＜ 0.05vs blank pair compared with blank control
According to)；Raspberry polysaccharide high dose group has pole significant difference (* * P ＜ 0.01vs blank control groups) compared with blank control.
(2) in raspberry Polysaccharides on Mice serum creatine kinase influence
The influence of creatine kinase in 17. raspberry Polysaccharides on Mice serum of table
* P ＜ 0.05, * * P ＜ 0.01vs blank control groups
Creatine kinase is closely related with energetic supersession, it participates in the control of glycolysis, mitochondrial respiratory machine contraction of muscle supplies
Energy.Research shows that after a large amount of movements, the creatine kinase activity in serum significantly increases.The variation of creatine kinase can be anti-in serum
Film projector body is to the adaptedness of movement, and adaptedness is higher, and serum creatine kinase activity, which can reduce, even restores normal level.By
Table 17 is as it can be seen that raspberry polysaccharide can be substantially reduced the content of creatine kinase in mice serum, and dose-dependence is presented.Wherein,
Raspberry polysaccharide middle dose group has significant difference (* P ＜ 0.05vs blank controls) compared with blank control；The high agent of raspberry polysaccharide
Amount group has pole significant difference (* * P ＜ 0.01vs blank control groups) compared with blank control.
(3) influence of raspberry Polysaccharides on Mice blood glucose
The influence of blood sugar in 18. raspberry Polysaccharides on Mice serum of table
* P ＜ 0.01vs blank control groups
As shown in table 18, raspberry polysaccharide can significantly increase the content of mice serum blood glucose, and dose-dependence is presented.Its
In, the high middle dose group of raspberry polysaccharide has pole significant difference (* * P ＜ 0.01vs blank control groups) compared with blank control.
(4) in raspberry Polysaccharides on Mice serum lactic acid (LD) content influence
The influence of lactic acid in 19. raspberry Polysaccharides on Mice serum of table
* P ＜ 0.01vs blank control groups
Lactic acid is generated by anaerobic metabolism, be there is the stability for destroying organismic internal environment, is influenced cardiovascular system, bone
The normal performance of musculation function leads to the generation of fatigue.Be shown in Table 19, raspberry polysaccharide it is each can pole significantly reduce mice serum
Lactic acid content (* * P ＜ 0.01vs blank control groups), and dose-dependence is presented.
(5) in raspberry Polysaccharides on Mice serum lactic dehydrogenase (LDH) content influence
The influence of lactic dehydrogenase in 20. raspberry Polysaccharides on Mice serum of table
* P ＜ 0.01vs blank control groups
Lactic acid in lactic dehydrogenase energy decomposer, remove in time internal lactic acid for fatigue elimination have it is very positive
Effect.As shown in table 20, raspberry polysaccharide can significantly increase the content of lactic dehydrogenase in mice serum, and dose-dependant pass is presented
System.Wherein, the high middle dose group of raspberry polysaccharide has (the * * P ＜ 0.01vs blank controls of pole significant difference compared with blank control
Group).
Above-mentioned experiment shows that raspberry polysaccharide has good anti-fatigue effect.
The preparation of 17 oral liquid of embodiment
1) it matches by mass percentage：
| Raspberry polysaccharide | 17% |
| Distilled water | 83% |
2) raspberry polysaccharide is weighed in proportion.Raspberry polysaccharide is added in heating in distilled water makes its dissolving, is uniformly mixed, through cold
In filling to 10 milliliters totally-enclosed Packaging Bottles, 121 DEG C sterilize 15 minutes to get finished product.
The preparation of 18 oral liquid of embodiment
1) it matches by mass percentage：
| Raspberry polysaccharide | 25% |
| Distilled water | 75% |
2) raspberry polysaccharide powder is weighed in proportion.The more powder of raspberry are added in heating in distilled water makes its dissolving, is uniformly mixed, warp
It cools down in filling to 10 milliliters totally-enclosed Packaging Bottles, 121 DEG C sterilize 15 minutes to get finished product.
Claims (9)
1. a kind of purposes of raspberry polyoses oral liquid in antitumor drug is prepared, the raspberry polyoses oral liquid is by as follows
The supplementary material of weight proportion is prepared：
Raspberry polysaccharide 15%~35%, remaining is distilled water；
Wherein, the raspberry polysaccharide is prepared using water extraction and alcohol precipitation method；The water extraction and alcohol precipitation method concrete operations are as follows：
It takes dry raspberry, after petroleum ether or ether defatting, is extracted with 75~95%v/v of concentration ethyl alcohol, filtering, filter residue adds water to carry
It takes, after water intaking extract concentration, ethyl alcohol to alcohol content is added to reach 75%~85%, cooling is stood, and it is that raspberry is more to take solid content
Sugar；
Wherein, the raspberry is the fruit of rose family rubus plant, and selected from raspberry or black raspberry.
2. purposes according to claim 1, it is characterised in that：The raspberry polyoses oral liquid is by following weight proportion
Supplementary material is prepared：
Raspberry polysaccharide 17%~25%, remaining is distilled water.
3. purposes according to claim 1 or claim 2, it is characterised in that：Raspberry polyoses oral liquid and preparation method thereof is as follows：
Raspberry polysaccharide is added in heating in distilled water makes its dissolving, is uniformly mixed, filling after cooling, sterilizes to get oral liquid.
4. purposes according to claim 1 or claim 2, it is characterised in that：In the raspberry polysaccharide, purity of polysaccharide is more than 50%.
5. purposes according to claim 4, it is characterised in that：Purity of polysaccharide is more than 80%.
6. purposes according to claim 5, it is characterised in that：Purity of polysaccharide is more than 85%.
7. purposes according to claim 6, it is characterised in that：Purity of polysaccharide is 85~95%w/w.
8. purposes according to claim 1, it is characterised in that：Remove seed or non-remove seed before raspberry extraction.
9. purposes according to claim 1, it is characterised in that：The tumour for melanoma, nasopharyngeal carcinoma, liver cancer, lung cancer,
Glioma, thyroid cancer, cancer of pancreas, the cancer of the esophagus, colon cancer, oophoroma or prostate cancer.
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