CN101358222A - Method for microwave auxiliarily extracting antiradiation polysaccharide using potentilla anserine as feedstock - Google Patents
Method for microwave auxiliarily extracting antiradiation polysaccharide using potentilla anserine as feedstock Download PDFInfo
- Publication number
- CN101358222A CN101358222A CNA2008101464879A CN200810146487A CN101358222A CN 101358222 A CN101358222 A CN 101358222A CN A2008101464879 A CNA2008101464879 A CN A2008101464879A CN 200810146487 A CN200810146487 A CN 200810146487A CN 101358222 A CN101358222 A CN 101358222A
- Authority
- CN
- China
- Prior art keywords
- under
- radiation
- hours
- polysaccharide
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a method for extracting radiation-resistant polysaccharide with the help of microwaves and with potentilla anserina L. as the raw material. The method is that the potentilla anserina L. is used as the raw material and the microwaves give the assistance to extract natural polysaccharide. The polysaccharide is mainly used for preparing a therapeutic agent which can treat radiation damages which are caused by X-ray and significantly repair the body injuries which are caused by radiation. The preparation which is prepared by the invention has no toxic side effects, good radiation-resistance effect and good efficacy to the radiation injuries which are caused by X-ray. Mice pharmacological experiments shows that taking the preparation for 30 days can enable the animals with radiation injuries to increase 32.1 percent in weight, 316.7 percent in thymus index, 210.8 percent in spleen index and 142.86 percent in the DNA content of bone marrow and reduce 58.3 percent in bone marrow micronucleus. The preparation of the invention can improve the function of the blood system and the hematopoietic system which are injured by radiation, and strengthen the body immunity.
Description
Technical field
The present invention relates to a kind of preparation method of polysaccharide, especially relating to a kind of is the method for raw material microwave auxiliarily extracting antiradiation polysaccharide with the fern fiber crops.
Background technology
Ionization radiation injury is to find and utilize the appearance afterwards of radioactive elements such as X ray, radium, thorium, uranium 19 end of the centurys.Effects of ionizing radiation is in organism, make the intravital molecule of machine, atom is ionized, excite, generate oxyradical etc., remove ability if surpass body, to cause the biomacromolecule lipid, nucleic acid, protein, enzyme, the destruction of carbohydrate etc., damaging cells plasma membrane and nucleus, disturb and destroy the cytokinetics normal procedure of the cell colony of bringing in constant renewal in, comprise inhibition, blocking-up and destruction cell eubolism, propagation, differentiation and ripening process, cause body from cell, form takes place on ubcellular and the molecular level, many-sided pathological change such as quantity and biochemistry, if fail correct the reparation, to cause lower immune function, the dna break damage, chromosome aberration, the structure and the dysfunction of the unusual and cell tissue of cell competence for added value heredity occurs and endanger.
Along with the progress of tumor radiotherapy and developing rapidly of nuclear industry, the research of antiradiation drug is come into one's own day by day.Mainly show from different levels and level the radioprotective active substance mechanism of action is furtherd investigate, the high radioprotective active substance of autotelic searching toxicity low activity.Patent CN1256084C discloses the application of Oleum Bulbus Allii in radiation-induced oligoleukocythemia and dna damage medication preparation; Patent CN1282455C discloses the antiradiation drug of a kind of ginkgolic flavone glycoside and tea-polyphenol combination; Patent CN1213669C disclose a kind of by Ganoderma spore powder make have radioprotective, Green Tea Extract and have the protection DNA functional health-care food.
Fern fiber crops (Potentilla anserina L.) are expanded the piece root for Rosaceae potentilla plants potentilla anserina, Tibetan language name " stamp agate ", and the Tibetan medicine claims " tall and erect wine was once ", belongs to per nnial herb.Though all there is the potentilla anserina growth in many areas, the whole nation, according to " Xinhua's book on Chinese herbal medicine outline " record " only in the Qinghai-Tibet Platean, there is the piece root development this kind beginning "." Tibet's economy plant " carries: " the sharp phlegm of cough-relieving is controlled various hemorrhage and diarrheas for all herbal medicine, astringing to arrest bleeding.The piece root is used as medicine, the effect of invigorating spleen and reinforcing stomach is arranged, kidney is supported in the cough-relieving of promoting the production of body fluid, benefiting vital QI for enriching blood, enriching yin; Cure mainly malnutrition, anaemia, insufficiency of the spleen diarrhoea, the illnesss such as gas loses of suffering from a deficiency of the kidney after being ill." it promptly can be used as medicine and be used for promoting health and curing diseases, and can be used as food again with the cuisines of allaying one's hunger.Show through modern scientific research: the Chemical Composition of fern fiber crops mainly contains the amino acid of multiple needed by human body such as various trace elements such as carbohydrate, protein, VitB1, nicotinic acid, tannin, zinc, manganese, copper and aspartic acid, Threonine, Serine, L-glutamic acid.
The fern fiber crops are rich in rich nutrient substances such as polysaccharide, starch and amino acid as the traditional medicine-food two-purpose plateau plant of China, have obtained in recent years paying close attention to widely.Patent CN 02138522.X has applied for a kind of protective foods and preparation thereof that contains the fern Nettle extract, adopts water and/or extraction using alcohol fern fiber crops, makes the effect that protective foods has middleman's body immunity function, antifatigue.Patent CN200710056499.8 has reported the application of fern fiber crops in the preparation anti-anoxic medicine; by integral body and cell levels anoxia model; observe the oxygen lack resistant function of Chinese medicine fern fiber crops; can significantly improve survival rate and the survival time of asphyxiating anoxic and acute decompression anoxic mouse; alleviate the myocardial damage of drug specificity anoxic mouse; neonatal rat myocardial cell and neurocyte anoxia-induced apoptosis to vitro culture also have significant provide protection, and the purposes of Chinese medicine fern fiber crops in the preparation anti-anoxic medicine is provided.
Problems such as at present, the facility investment of conventional extraction method of polysaccharides ubiquity is bigger, and running cost height and extraction yield are lower.And the microwave-assisted extraction mechanism of action is different from other extracting method, microwave technology is that the quick concussion of 24.5 hundred million ultra-high frequencies of per second that produces by magnetron makes especially polarizable medium generation concussion at a high speed of solvent molecule, spin, increased the ability that solvent enters solute inside, utilize molecular polarization or ionic conduction effect and directly material is heated, the volume heating of microwave adds heat energy with selectivity changes the microscopic structures of raw material, make that target component is easier to be discharged from matrix, make whole extraction time shorten, extraction yield increases, and because the penetration power of microwave also makes the granularity requirements that raw material is handled reduce.Patent CN 1880342A discloses a kind of extracting polysaccharide from astragalus root using microwave method, extracts astragalus polysaccharides under microwave power 200W, constant temperature, and extraction yield is 9%-20%; Patent CN 1246320C has reported a kind of technology of microwave method extraction matrine, extracts with polar organic solvent under the 12-36W/L microwave intensity, and matrine reaches more than 0.22%; Patent CN 1109028C has reported the method for a kind of microwave extraction ginkgolic flavone glycoside and bilobalide-like substances, adopt microwave power at 500-1500W, extract in the mixture of water and organic solvent, the content of Ginkgo total flavones and lactone is 38-40%/10-12% in the extract; Patent CN1872883's a kind of preparation method of radiation resistant fern amylose has reported for work, and this patent is the raw material ultrasonic auxiliary extraction method with the fern fiber crops, has obtained significantly strengthening the polysaccharide of animal capability of resistance to radiation.
The extracting method of the fern amylose of mentioning in present document is generally conventional hot water extraction, does not relate to microwave-assisted and extracts.Therefore, a kind of method of using microwave-assisted to extract fern amylose of invention is a purpose of the present invention.
Summary of the invention
It is the method for raw material microwave auxiliarily extracting antiradiation polysaccharide with the fern fiber crops that the technical problem to be solved in the present invention provides a kind of.Be raw material with the fern fiber crops promptly, microwave-assisted extracts natural polysaccharide, and this polysaccharide is mainly used in the radiation injury that preparation treatment X-ray causes, and radiation-induced body injury is had the therapeutical agent of tangible reparation.
For solving the problems of the technologies described above, the present invention is a kind of to be the method for raw material microwave auxiliarily extracting antiradiation polysaccharide with the fern fiber crops, comprises the steps:
1) takes by weighing a certain amount of fern fiber crops, place Microwave Extraction Equipment, add the distilled water of 10～40 times of fern fiber crops weight, stirring, mixing; In microwave frequency is 915MHz, and power is to extract 45～90 minutes under the condition of 200W～400W, and it is 60 ℃～80 ℃ that temperature is extracted in control; Suction filtration under the condition of 0.06～0.08MPa is collected filtrate; With filtrate concentrating under reduced pressure under 0.06～0.08Mpa,, get concentrated solution to 1/8～1/4 of former filtrate volume; Add ethanol in concentrated solution, described ethanol content accounts for and adds 70～80% of overall solution volume behind the ethanol, leaves standstill under the room temperature 20～30 hours, and is centrifugal under 3500～4000 rev/mins rotating speed then, collecting precipitation; At-60～-50 ℃, lyophilize is 30～36 hours under the condition under the 1-5Pa with throw out, gets substance A;
2) take by weighing measured quantity of material A, water dissolution with its 5～8 times of weight, heating contains the aqueous solution to 50～60 ℃ of substance A, add papoid 5u～7u/100mlA aqueous solution then and carried out enzymolysis 8～10 hours, extract 2～4 times with propyl carbinol and chloroform mixed solution, the volume ratio of described propyl carbinol and chloroform is 1: 2～4 again; Collect upper water solution, under 40～50 ℃, 0.06-0.08Mpa, be evaporated to 1/10 of former aqueous solution volume～1/12 o'clock, substance B;
3) drip strong aqua in substance B, transfer to ph=8-9, dropping concentration is 20～30% H
2O
2The aqueous solution is become faint yellowly by sorrel until solution, be evaporated to 1/8～1/4 of original solution volume then under the condition of 0.06～0.08Mpa, pours in the dialysis tubing of molecular weight cut-off 8000～14000Da and dialyses 40～48 hours; In dialysis tubing, add ethanol, ethanol content is accounted for add 70～80% of overall solution volume behind the ethanol, left standstill 10～14 hours; Centrifugation under 3500～4000 rev/mins rotating speed, collecting precipitation; At-60～-50 ℃, lyophilize is 30～36 hours under the condition under the 1-5Pa with throw out, gets the fern amylose product of purifying.
The product ultraviolet absorption spectroscopy of the present invention's preparation: the fern amylose behind the purifying has strong absorption peak through UV scanning at the 200nm place, at equal free nucleic acid of 260nm, 280nm and proteinic charateristic avsorption band, impurity such as not containing nucleic acid and protein is described; Ninhydrin reaction, IKI reaction and biuret reaction are all negative, and no protein and starch are described; Infrared spectra is presented at 2931cm
-1There is a characteristic peak at the place, is that the angle vibration of c h bond in the molecule causes; At 3425cm
-1There is a characteristic peak at the place, is that the stretching vibration of O-H causes, these are the absorption peak of polysaccharide; Fehling reagent reaction and sulfuric acid-phynol are reacted into the positive, illustrate that fern amylose is the reductibility polysaccharide.The viscosity-average molecular weight of utilizing viscosimetry to measure fern amylose is: (1.05～2) * 10
6
Extracting method of the present invention and traditional extraction process are relatively
| The experiment number | Solid-liquid ratio | Extraction time (min) | The extraction temperature (℃) | Microwave power (W) | Extraction yield (%) |
| Experiment 1 | 1∶10 | 70 | 80 | 200 | 10.86 |
| Experiment 2 | 1∶20 | 45 | 60 | 400 | 12.18 |
| Experiment 3 | 1∶40 | 90 | 70 | 300 | 11.24 |
| Comparative example | 1∶50 | 180 | 90 | - | 5.73 |
Conclusion: preparation method of the present invention can increase substantially extraction yield than traditional method.
The product radioprotective effect animal experiment of the present invention's preparation is as follows:
50 of Kunming mouses of experiment, male and female half and half, be divided into 5 groups, every group 10, as follows respectively: dosage group (M, 150mg/ (kgb.w.)), polysaccharide low dose group (L in normal control group (Control), model group (Model), polysaccharide high dose group (H, 300mg/ (kgb.w.)), the polysaccharide, 75mg/ (kgb.w.)), the normal control group is irritated stomach physiological saline.Administration is 20 days before the radiation, and administration in the 1h after the radiation is administered once and fixing administration time later every day, observes the animals survived situation in 30 days.Illuminate condition is: X ray, mouse single whole body dose 4Gy, dose rate 1Gy/min, ource-skin Distance 1m.Except that the normal control group was not shone, other groups were all shone.X ray reduces mouse body weight, peripheral blood leucocyte, thymus index, index and spleen index and marrow dna content, and the marrow micronuclear rates raises.
Normal mouse body weight body weight in experimentation continues to increase; And model group mouse body weight is on a declining curve all the time after radiation.The animal that gives this preparation is all on a declining curve back 10 days of irradiation, recovers body weight after 10 days gradually and begins to increase, and wherein high dose group body weight amplification is 12.87%, apparently higher than model group.Illustrate that this preparation can alleviate the reduction of the mouse body weight that radiation causes.
Mouse is through after the x-ray bombardment, and the marrow dna content sharply descends, and normally reaches utmost point conspicuous level (P＜0.01) without irradiation group mouse ratio.Irritate the mouse group of this preparation of stomach before and after radiation, dna content is compared all with model group and is improved, and wherein middle dosage group reaches extremely significant level (P＜0.01).Illustrate that this preparation has significant protective effect to radiation-induced mouse bone marrow cells dna content decline.
The normal control mice PCE micronuclear rates (4.67 ‰) of mouse PCE micronuclear rates (41.62 ‰) sharply increases after the radiation.Through overshoot and the mouse PCE micronuclear rates that gives this preparation decline is arranged all; wherein middle dosage group mouse PCE micronuclear rates drops to 17.34 ‰; compare with model group and to reach utmost point conspicuous level (P＜0.01), illustrate that this preparation has significant protection and repair to radiation to the damage of hemopoietic system.Obvious atrophy appears in mouse spleen and thymus gland after the radiation, and lymphopoiesis obviously descends.Through the tangible rise that all has been significantly improved of overshoot and the spleen, thymic weight and the lymphopoiesis that give the mouse of this preparation, illustrate that the immunity system after this preparation is to radiation injury has restitution, illustrate that this preparation has radioprotective effect preferably.
Beneficial effect of the present invention is as follows: the present invention is with fern fiber crops raw material, through steps such as microwave extraction, purifying, decolourings, prepare a kind of natural radioprotective preparation, said preparation has no side effect, radioprotective is effective, and the radiation injury that X ray is caused has curative effect preferably.Through the mouse pharmacological evaluation is shown: took the body weight rising 32.1% that said preparation makes the radiation injury animal in 30 days, thymus index rising 316.7%, index and spleen index rising 210.8%, marrow dna content rising 142.86%, marrow micronuclear rates reduce by 58.3%; This product formulation can be improved blood system, the hemopoietic system function behind the radiation loss, enhancing body immunizing power.
The present invention can make full use of the domestic a large amount of fern fiber crops that distribute in ground such as China Gansu, Qinghai, Inner Mongolia Autonomous Region, and good resources advantage is arranged, the produce market promise well of the present invention's preparation, and economic benefit is wide.
Description of drawings
Below in conjunction with accompanying drawing the specific embodiment of the present invention is described in further detail.
Fig. 1 is the influence of this preparation to the radiation murine body weight;
Fig. 2 is the influence of this preparation to the radiation murine index and spleen index;
Fig. 3 is the influence of this preparation to the radiation murine thymus index;
Fig. 4 is the influence of this preparation to radiation murine marrow micronuclear rates;
Fig. 5 is that this preparation influences the radiation murine dna content;
The UV scanning figure of this preparation of Fig. 6;
The infrared scan figure of this preparation of Fig. 7.
Embodiment
Embodiment 1
A kind of is the method for raw material microwave auxiliarily extracting antiradiation polysaccharide with the fern fiber crops, comprises the steps:
1) takes by weighing a certain amount of fern fiber crops, place Microwave Extraction Equipment, add the distilled water of 10 times of fern fiber crops weight, stirring, mixing; In microwave frequency is 915MHz, and power is to extract 45 minutes under the condition of 200W, and it is 80 ℃ that temperature is extracted in control; Suction filtration under the condition of 0.08MPa is collected filtrate; With filtrate concentrating under reduced pressure under 0.08Mpa,, get concentrated solution to 1/4 of former filtrate volume; Add ethanol in concentrated solution, described ethanol content accounts for and adds 70% of overall solution volume behind the ethanol, leaves standstill under the room temperature 20 hours, and is centrifugal under 3500 rev/mins rotating speed then, collecting precipitation; At-50 ℃, lyophilize is 30 hours under the condition under the 1Pa with throw out, gets substance A;
2) take by weighing measured quantity of material A, with the water dissolution of 5 times of weight, heating contains the aqueous solution to 50 ℃ of substance A, adds the 5u/100ml papoid then and carries out enzymolysis 8 hours, extract 3 times with propyl carbinol and chloroform mixed solution, the volume ratio of described propyl carbinol and chloroform is 1: 4 again; Collect upper water solution, under 45 ℃, 0.07Mpa, be evaporated to 1/10 o'clock of former aqueous solution volume, substance B;
3) drip strong aqua in substance B, transfer to ph=8, dropping concentration is 20% H
2O
2The aqueous solution is become faint yellowly by sorrel until solution, be evaporated to 1/8 of original solution volume then under the condition of 0.07Mpa, pours in the dialysis tubing of molecular weight cut-off 8000～14000Da dialysis then into 40 hours; In dialysis tubing, add ethanol, ethanol content is accounted for add 75% of overall solution volume behind the ethanol, left standstill 10 hours; Centrifugation under 4000 rev/mins rotating speed, collecting precipitation; At-55 ℃, lyophilize is 30 hours under the condition under the 1Pa with throw out, gets the fern amylose product of purifying.
Embodiment 2
A kind of is the method for raw material microwave auxiliarily extracting antiradiation polysaccharide with the fern fiber crops, comprises the steps:
1) takes by weighing a certain amount of fern fiber crops, place Microwave Extraction Equipment, add the distilled water of 30 times of fern fiber crops weight, stirring, mixing; In microwave frequency is 915MHz, and power is to extract 60 minutes under the condition of 300W, and it is 70 ℃ that temperature is extracted in control; Suction filtration under the condition of 0.06MPa is collected filtrate; With filtrate concentrating under reduced pressure under 0.06Mpa,, get concentrated solution to 1/8 of former filtrate volume; Add ethanol in concentrated solution, described ethanol content accounts for and adds 75% of overall solution volume behind the ethanol, leaves standstill under the room temperature 25 hours, and is centrifugal under 4000 rev/mins rotating speed then, collecting precipitation; At-55 ℃, lyophilize is 34 hours under the condition under the 5Pa with throw out, gets substance A;
2) take by weighing measured quantity of material A, with the water dissolution of 6 times of weight, heating contains the aqueous solution to 55 ℃ of substance A, adds the 6u/100ml papoid then and carries out enzymolysis 9 hours, extract 4 times with propyl carbinol and chloroform mixed solution, the volume ratio of described propyl carbinol and chloroform is 1: 2 again; Collect upper water solution, under 40 ℃, 0.06Mpa, be evaporated to 1/12 o'clock of former aqueous solution volume, substance B;
3) drip strong aqua in substance B, transfer to ph=9, dropping concentration is 30% H
2O
2The aqueous solution is become faint yellowly by sorrel until solution, be evaporated to 1/5 of original solution volume then under the condition of 0.08Mpa, pours in the dialysis tubing of molecular weight cut-off 8000～14000Da dialysis then into 44 hours; In dialysis tubing, add ethanol, ethanol content is accounted for add 80% of overall solution volume behind the ethanol, left standstill 14 hours; Centrifugation under 3800 rev/mins rotating speed, collecting precipitation; At-50 ℃, lyophilize is 36 hours under the condition under the 5Pa with throw out, gets the fern amylose product of purifying.
Embodiment 3
A kind of is the method for raw material microwave auxiliarily extracting antiradiation polysaccharide with the fern fiber crops, comprises the steps:
1) takes by weighing a certain amount of fern fiber crops, place Microwave Extraction Equipment, add the distilled water of 40 times of fern fiber crops weight, stirring, mixing; In microwave frequency is 915MHz, and power is to extract 90 minutes under the condition of 400W, and it is 60 ℃ that temperature is extracted in control; Suction filtration under the condition of 0.07MPa is collected filtrate; With filtrate concentrating under reduced pressure under 0.07Mpa,, get concentrated solution to 1/6 of former filtrate volume; Add ethanol in concentrated solution, described ethanol content accounts for and adds 80% of overall solution volume behind the ethanol, leaves standstill under the room temperature 30 hours, and is centrifugal under 3800 rev/mins rotating speed then, collecting precipitation; At-60 ℃, lyophilize is 36 hours under the condition under the 3Pa with throw out, gets substance A;
2) take by weighing measured quantity of material A, with the water dissolution of 8 times of weight, heating contains the aqueous solution to 60 ℃ of substance A, adds the 7u/100ml papoid then and carries out enzymolysis 10 hours, extract 2 times with propyl carbinol and chloroform mixed solution, the volume ratio of described propyl carbinol and chloroform is 1: 3 again; Collect upper water solution, under 50 ℃, 0.08Mpa, be evaporated to 1/11 o'clock of former aqueous solution volume, substance B;
3) drip strong aqua in substance B, transfer to ph=8, dropping concentration is 25% H
2O
2The aqueous solution is become faint yellowly by sorrel until solution, be evaporated to 1/4 of original solution volume then under the condition of 0.06Mpa, pours in the dialysis tubing of molecular weight cut-off 8000～14000Da dialysis then into 48 hours; In dialysis tubing, add ethanol, ethanol content is accounted for add 70% of overall solution volume behind the ethanol, left standstill 12 hours; Centrifugation under 3500 rev/mins rotating speed, collecting precipitation; At-60 ℃, lyophilize is 36 hours under the condition under the 3Pa with throw out, gets the fern amylose product of purifying.
Obviously, the above embodiment of the present invention only is for example of the present invention clearly is described, and is not to be qualification to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here can't give exhaustive to all embodiments.Everyly belong to the row that conspicuous variation that technical scheme of the present invention extends out or change still are in protection scope of the present invention.
Claims (1)
1, a kind of is the method for raw material microwave auxiliarily extracting antiradiation polysaccharide with the fern fiber crops, it is characterized in that, comprises the steps:
1) takes by weighing a certain amount of fern fiber crops, place Microwave Extraction Equipment, add the distilled water of 10～40 times of fern fiber crops weight, stirring, mixing; In microwave frequency is 915MHz, and power is to extract 45～90 minutes under the condition of 200W～400W, and it is 60 ℃～80 ℃ that temperature is extracted in control; Suction filtration under the condition of 0.06～0.08MPa is collected filtrate; With filtrate concentrating under reduced pressure under 0.06～0.08Mpa,, get concentrated solution to 1/8～1/4 of former filtrate volume; Add ethanol in concentrated solution, described ethanol content accounts for and adds 70～80% of overall solution volume behind the ethanol, leaves standstill under the room temperature 20～30 hours, and is centrifugal under 3500～4000 rev/mins rotating speed then, collecting precipitation; At-60～-50 ℃, lyophilize is 30～36 hours under the condition under the 1-5Pa with throw out, gets substance A;
2) take by weighing measured quantity of material A, water dissolution with its 5～8 times of weight, heating contains the aqueous solution to 50～60 ℃ of substance A, add papoid 5u～7u/100mlA aqueous solution then and carried out enzymolysis 8～10 hours, extract 2～4 times with propyl carbinol and chloroform mixed solution, the volume ratio of described propyl carbinol and chloroform is 1: 2～4 again; Collect upper water solution, under 40～50 ℃, 0.06-0.08Mpa, be evaporated to 1/10 of former aqueous solution volume～1/12 o'clock, substance B;
3) drip strong aqua in substance B, transfer to ph=8-9, dropping concentration is 20～30% H
2O
2The aqueous solution is become faint yellowly by sorrel until solution, be evaporated to 1/8～1/4 of original solution volume then under the condition of 0.06～0.08Mpa, pours in the dialysis tubing of molecular weight cut-off 8000～14000Da and dialyses 40～48 hours; In dialysis tubing, add ethanol, ethanol content is accounted for add 70～80% of overall solution volume behind the ethanol, left standstill 10～14 hours; Centrifugation under 3500～4000 rev/mins rotating speed, collecting precipitation; At-60～-50 ℃, lyophilize is 30～36 hours under the condition under the 1-5Pa with throw out, gets the fern amylose product of purifying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101464879A CN101358222A (en) | 2008-09-01 | 2008-09-01 | Method for microwave auxiliarily extracting antiradiation polysaccharide using potentilla anserine as feedstock |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101464879A CN101358222A (en) | 2008-09-01 | 2008-09-01 | Method for microwave auxiliarily extracting antiradiation polysaccharide using potentilla anserine as feedstock |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101358222A true CN101358222A (en) | 2009-02-04 |
Family
ID=40330820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008101464879A Pending CN101358222A (en) | 2008-09-01 | 2008-09-01 | Method for microwave auxiliarily extracting antiradiation polysaccharide using potentilla anserine as feedstock |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101358222A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169789A (en) * | 2012-12-18 | 2013-06-26 | 甘肃农业大学 | Method for extracting total flavonoid in silverweed cinquefoil root |
| CN103356691A (en) * | 2013-07-22 | 2013-10-23 | 兰州理工大学 | Preparation method of potentilla anserina L. root crude polysaccharide with potentilla anserina L. root as raw material and use |
| CN104231096A (en) * | 2013-06-13 | 2014-12-24 | 段玉虎 | Ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide |
-
2008
- 2008-09-01 CN CNA2008101464879A patent/CN101358222A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169789A (en) * | 2012-12-18 | 2013-06-26 | 甘肃农业大学 | Method for extracting total flavonoid in silverweed cinquefoil root |
| CN104231096A (en) * | 2013-06-13 | 2014-12-24 | 段玉虎 | Ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide |
| CN104231096B (en) * | 2013-06-13 | 2016-08-10 | 段玉虎 | A kind of method using ultrasonic assistant defat to extract Radix potentillae anserinae polysaccharide |
| CN103356691A (en) * | 2013-07-22 | 2013-10-23 | 兰州理工大学 | Preparation method of potentilla anserina L. root crude polysaccharide with potentilla anserina L. root as raw material and use |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103284152B (en) | Dietary fiber extracted from Chinese yew, extraction method and use | |
| CN106084080A (en) | A kind of Radix Glycyrrhizae selenium polysaccharide and preparation method thereof | |
| CN102304501A (en) | Complex enzyme preparation and application and method thereof for extracting plant polysaccharides by using same | |
| CN105504079A (en) | Process for producing astragalus polysaccharide by using ultrasonic technology | |
| CN112870236A (en) | Flavone effective part of abelmoschus manihot and preparation method and application thereof | |
| CN102119963A (en) | Extract for preventing and treating angina pectoris and coronary heart disease and preparation method and application thereof | |
| CN102119964A (en) | Extract of preventing and treating coronary heart disease and stenocardia and preparation method and application thereof | |
| CN101358222A (en) | Method for microwave auxiliarily extracting antiradiation polysaccharide using potentilla anserine as feedstock | |
| CN103830292A (en) | Apocynum flavonoid extract and preparation method thereof | |
| CN102370695A (en) | Qinglongyi active extract, its preparation method and its application | |
| CN101850032A (en) | Anti-tumor traditional Chinese medicine composition and preparation method and application thereof | |
| CN101875648B (en) | Method for extracting and purifying Pinostrobin from medicinal plants, and pharmaceutical preparation and application thereof | |
| CN105777921A (en) | Astragalus polysaccharide extraction process and application thereof | |
| CN101619108B (en) | Method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves | |
| CN1806831B (en) | Medicine for treating psoriasis and its preparing process | |
| CN101879209A (en) | Oral liquid for strengthening healthy energy and relieving internal heat or fever for animals | |
| CN105330670A (en) | Method for continuously efficiently extracting parthenolide from southern magnolia leaves | |
| CN109172637B (en) | Preparation method of capsule for treating rheumatism | |
| CN102119952A (en) | Ultrasonic extraction preparation method of Chinese traditional medicine preparation for treating lumbago due to kidney deficiency | |
| CN102038813A (en) | Coconut palm leaf extract and preparation method and use thereof | |
| CN110917232A (en) | Method for applying medical natural product targeted medicine of dandelion extracted by hydrothermal method to mainly treat dermatitis allergy | |
| CN104840748B (en) | Chinese medicine composition with anti-cancer of the brain activity and its preparation method and application | |
| CN104740181B (en) | A kind of Chinese medicine preparation for treating dam postpartum retention of afterbirth and preparation method thereof | |
| CN107582774A (en) | One kind treats rheumatalgia pain monkshood dip and preparation method thereof | |
| CN104274538B (en) | Traditional Chinese medicine extract for treating gout and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20090204 |