CN104840748B - Chinese medicine composition with anti-cancer of the brain activity and its preparation method and application - Google Patents
Chinese medicine composition with anti-cancer of the brain activity and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine composition with anti-cancer of the brain activity and preparation method thereof and its application, the Traditional Chinese medicine compound composition is by 10 ~ 30 parts of processed rhizoma typhonii or radix aconiti corean, 15~45 parts of edible tulip, 3~9 parts of centipede, 10~30 parts of Rhizoma Arisaematis (processed), 10~30 parts of Herba Lycopi, 10~30 parts of rhizoma alismatis are made.Test result indicates that, Chinese medicine composition provided by the invention with anti-cancer of the brain activity, match scientific and reasonable, medication is rigorous, has anticancer removing toxic substances, eleminating phlegm and freeing channels and other effects, inside and outside is respectively provided with brain cancer cell strong killing effect, and the generation of tumor necrosis factor can be promoted, tumor patient immunity is improved, and it is safe, toxic side effect is low.Preparation method provided by the invention, stable quality, effective component extracting content is high, workable, is adapted to industrialized production.
Description
Technical field
The present invention relates to a kind of Chinese medicine, and in particular to a kind of Chinese medicine composition with anti-cancer of the brain activity and preparation method thereof
And application.
Background technology
Intracranial tumors are also known as brain tumor, its unknown etiology as yet, and tumour occurs from brain, meninx, pituitary, cranial nerve, brain
Blood vessel and embryonal rest organizer, are known as Primary intracranial tumor.By the Malignant tumor of bonal metastasis of the other organs and tissues of body to cranium
Interior person, is known as Secondary cases intracranial tumors.
The global statistics of intracranial tumors illness rate one is 40/,100,000.Illustrate that the incidence of intracranial tumors is not low, be worth
It must pay attention to.In the incidence of general tumour, brain tumor occupies the 5th (6.31%), is only below stomach, uterus, mammary gland, esophagus swell
Knurl.It is being grown up, brain tumor accounts for the 2% of general tumour sum.Clinically the medicine of the treatment cancer of the brain is mainly chemicals at present, but by
It is big in chemotherapeutics toxicity, while tumour cell is killed also the human body such as the kill leucocyte of non-selectivity, lymphocyte from
Body immunocyte, causes patient usually since because autoimmunity inferior capabilities are complicated by infection, patient uses erious adverse reaction, and treats
Journey is grown, costly, there is larger limitation in Clinical practice.
Traditional Chinese medicine has unique advantage in terms of tumour is treated, and the natural class medicine using Chinese medicine as representative has poison is secondary to make
With the features such as small, action target spot is various, better tolerance, more and more it is taken seriously in terms of tumour is treated.But report at present
Anti- cancer of the brain drug medication amount is larger, and impurity component is more, works slower.
Therefore, a kind of anti-cancer of the brain effect of research and development is good, and dosage is few, has good therapeutic effect to the cancer of the brain, and
The low antineoplastic Chinese traditional medicine of adverse reaction is of great significance.
The content of the invention
Goal of the invention：Present invention aims to solve the deficiencies of the prior art, and provides a kind of one kind proportioning it is scientific and reasonable, curative effect is true
Cut, Clinical practice safety, the low Chinese medicine composition with anti-cancer of the brain activity of adverse reaction, another object of the present invention is to provide
The preparation method of the Chinese medicine composition with anti-cancer of the brain activity and its application in treatment cancer of the brain disease medicament is prepared.
Technical solution：In order to realize the above object the technical solution that the present invention takes is：
A kind of Chinese medicine composition with anti-cancer of the brain activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：
10~30 parts of processed rhizoma typhonii or radix aconiti corean, 15~45 parts of edible tulip, 3~9 parts of centipede, 10~30 parts of Rhizoma Arisaematis (processed), Herba Lycopi 10~30
Part, 10~30 parts of rhizoma alismatis.
Preferably, the above-described Chinese medicine composition with anti-cancer of the brain activity, it is by following parts by weight
Bulk pharmaceutical chemicals are made：10~20 parts of processed rhizoma typhonii or radix aconiti corean, 15~30 parts of edible tulip, 3~6 parts of centipede, 10~20 parts of Rhizoma Arisaematis (processed), Herba Lycopi 10~
20 parts, 10~20 parts of rhizoma alismatis.
Preferably, the above-described Chinese medicine composition with anti-cancer of the brain activity, it is by following parts by weight
Bulk pharmaceutical chemicals are made：10 parts of processed rhizoma typhonii or radix aconiti corean, 15 parts of edible tulip, 3 parts of centipede, 10 parts of Rhizoma Arisaematis (processed), 10 parts of Herba Lycopi, 10 parts of rhizoma alismatis.
Preferably, the above-described Chinese medicine composition with anti-cancer of the brain activity, by the extract of each bulk pharmaceutical chemicals
The medicine of oral liquid, granule, capsule or Tabules is prepared into pharmaceutically acceptable carrier.
The preparation method of Chinese medicine composition of the present invention with anti-cancer of the brain activity, it comprises the following steps：
(1) processed rhizoma typhonii or radix aconiti corean is taken in parts by weight, and edible tulip, Rhizoma Arisaematis (processed), Herba Lycopi, rhizoma alismatis, adds total 6~15 times of medicinal material weight
The ethanol of volume, then ultrasonic when immersion 24~48 is small, reflux or continuous circumfluence extraction 2~3 times, every time 1~3 it is small when,
Merge extracting solution, concentration, macroporous resin column on concentrate, successively with water, the ethanol elution of 20% ethanol~60%, collect 40~
50% ethanol eluate, concentration, obtains extract；
(2) take centipede in parts by weight, with petroleum ether continuous circumfluence extraction 2~3 times, every time 1~3 it is small when, obtain petroleum ether
Extracting solution, is concentrated and dried, obtains Scolopendra extract；
(3) extract for taking step (1) to obtain, the Scolopendra extract and pharmaceutically acceptable carrier that step (2) obtains
It is prepared into the medicine of oral liquid, granule, capsule or Tabules.
Application of the Chinese medicine composition of the present invention with anti-cancer of the brain activity in anti-cancer of the brain medicine is prepared.
When the extract of each raw material of Chinese medicine composition provided by the invention with anti-cancer of the brain activity is made oral liquid,
Add distilled water to dissolve the extract of each raw material, filter, add syrup, adjust pH value, oral liquid is made.
When the extract of each raw material of Chinese medicine composition provided by the invention with anti-cancer of the brain activity is made tablet,
The extract and lactose or cornstarch of each raw material, it is necessary to when add magnesium stearate lubricant, be uniformly mixed, whole grain, is then pressed
Tablet is made in piece.
Handle when capsule is made in the extract of each raw material of Chinese medicine composition provided by the invention with anti-cancer of the brain activity
The extract and carrier lactose or cornstarch of each raw material are uniformly mixed, and whole grain is then encapsulated that capsule is made.
When granule is made in the extract of each raw material of Chinese medicine composition provided by the invention with anti-cancer of the brain activity,
The extract and diluent lactose or cornstarch of each raw material are uniformly mixed, whole grain, dry, and granule is made.
Chinese medicine composition provided by the invention with anti-cancer of the brain activity, processed rhizoma typhonii or radix aconiti corean are acrid in flavour and warm in nature；It is poisonous.Return stomach, Liver Channel.
Function is with curing mainly：Wind-dispelling phlegm, arresting convulsion are jerked, detoxicating and resolving a mass analgesic.For heap soil or fertilizer over and around the roots of apoplexy phlegm, facial paralysis, phlegm and headache, polarization head
Bitterly, larynx numbness pharyngalgia, lockjaw；Venomous snake bite etc..
Edible tulip nature and flavor：It is sweet in flavor；Micro-pungent；It is cold in nature；Return liver warp；Stomach；Lung channel.With clearing heat and detoxicating；Dispersing swelling and dissipating binds act on,
With treatment ulcer malignant sore；Scrofula tuberculosis；Pharyngalgia larynx numbness effect.
Rhizoma Arisaematis (processed) is rhizoma arisaematis processed product, bitterly, warm-natured, people's lung, liver, the spleen channel, function：It is eliminating dampness and eliminating phlegm, dispelling wind and relieving convulsion,
Dissolving lump and resolving mass, Repercusion analgesia.
Herba Lycopi's nature and flavor slightly warm in nature, it is bitter, pungent.Major function is promoting blood circulation and removing blood stasis, line water detumescence.For irregular menstruation, Amenorrhea, pain
Through, postpartum blood stasis abdominal pain,
Centipede nature and flavor：Acrid flavour；It is warm-natured；It is poisonous.Channel tropism：Liver Channel.Effect：Expelling wind and relieving convulsion；Remove obstruction in channels to relieve pain；Dispersing pathogen accumulation.It is main
Control：Infantile convulsion, epilepsy；Spasm is twitched；Apoplexy；Lockjaw；Rheumatoid arthritis stubborn；Migraine and general headache；Venomous snake bite；Sore；Scrofula.
Rhizoma alismatis nature and flavor：It is sweet, light, cold in nature；Channel tropism：Return kidney channel, bladder warp；Effect：Clearing damp and promoting diuresis, expel the heat-evil it is treating stranguria；Cure mainly：It is small
Just unfavorable, puckery pain of heat gonorrhea, oedema turgor, diarrhea, phlegmatic vertigo, seminal emission.
Above all medicines share has the removing toxic substances of good anticancer, dissipating phlegm and removing blood stasis and other effects, available for the strongly fragrant poison of wind phlegm, deficiency of both qi and yin
The treatment of caused cancer of the brain disease.
Beneficial effect：Chinese medicine composition provided by the invention with anti-cancer of the brain activity is compared to the prior art with following
Advantage：
1st, for the present invention according to traditional Chinese medical theory, theoretical using diagnosis and treatment carries out Chinese prescription screening, there is provided have
The Chinese medicine composition proportioning of anti-cancer of the brain activity is scientific and reasonable, and medication is rigorous, has the work(such as good anticancer removing toxic substances, dissipating phlegm and removing blood stasis
Effect.Test result indicates that the Chinese medicine composition provided by the invention with anti-cancer of the brain activity suppresses brain cancer cell with good
Growth and the effect that brain tumor Apoptosis can be promoted.
2nd, test result indicates that the Chinese medicine composition provided by the invention with anti-cancer of the brain activity with righting reinforcing and is exempted from
Epidemic disease function facilitation, plays Mutiple Targets, and multiple organ treats the effect of tumour, can reduce tumor patient pain, extend life cycle,
The effect of life in patients is improved, and is shown through long-term experiment research, the Chinese medicine provided by the invention with anti-cancer of the brain activity
Composition is safe, and toxic side effect is low.
3rd, the extract of the Chinese medicine composition provided by the invention with anti-cancer of the brain activity can conveniently and pharmaceutical carriers system
It is standby into a variety of formulations, it is clinically convenient to take；The preparation of Chinese medicine composition provided by the invention with anti-cancer of the brain activity at the same time
Method, it is workable, design extraction and purification process, obtained extraction especially in accordance with each herbal medicine active ingredient chemical property
Thing active constituent content is high, and impurity is few, and has the advantages that absorption is fast, can play drug effect rapidly, and clinically dosage is few, and work
Skill is stablized, and is adapted to industrialized production.
Embodiment：
With reference to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments be merely to illustrate the present invention and
It is not used in and limits the scope of the invention, after the present invention has been read, various of equal value shapes of the those skilled in the art to the present invention
The modification of formula falls within the application appended claims limited range.
Implementation column 1
1. a kind of Chinese medicine composition with anti-cancer of the brain activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：RHIZOMA TYPHONII PREPARATA
10 grams of son, 15 grams of edible tulip, 3 grams of centipede, 10 grams of Rhizoma Arisaematis (processed), 10 grams of Herba Lycopi, 10 grams of rhizoma alismatis.
2. the preparation method of the Chinese medicine composition of the present invention with anti-cancer of the brain activity, it comprises the following steps：
(1) 10 grams of processed rhizoma typhonii or radix aconiti corean is taken in parts by weight, 15 grams of edible tulip, 10 grams of Rhizoma Arisaematis (processed), 10 grams of Herba Lycopi, 10 grams of rhizoma alismatis,
Add the ethanol of total 15 times of volumes of medicinal material weight, then ultrasonic when immersion 48 is small, continuous circumfluence extraction 3 times, every time 1 it is small when,
Merge extracting solution, concentration, macroporous resin column on concentrate, successively with water, the ethanol elution of 20% ethanol~60%, collect 40~
50% ethanol eluate, concentration, obtains extract；
(2) take 3 grams of centipede in parts by weight, with petroleum ether continuous circumfluence extraction 3 times, every time 2 it is small when, obtain petroleum ether extraction
Liquid, is concentrated and dried, obtains Scolopendra extract；
(3) extract for taking step (1) to obtain, the Scolopendra extract that step (2) obtains add lactose, and cornstarch mixes
Close uniformly, add magnesium stearate lubricant, be uniformly mixed, tablet is made in whole grain, then tabletting.(lot number：001)
Implementation column 2
1. a kind of Chinese medicine composition with anti-cancer of the brain activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：RHIZOMA TYPHONII PREPARATA
20 grams of son, 30 grams of edible tulip, 6 grams of centipede, 20 grams of Rhizoma Arisaematis (processed), 20 grams of Herba Lycopi, 20 grams of rhizoma alismatis.
2. the preparation method of the Chinese medicine composition of the present invention with anti-cancer of the brain activity, it comprises the following steps：
(1) 20 grams of processed rhizoma typhonii or radix aconiti corean is taken in parts by weight, 30 grams of edible tulip, 20 grams of Rhizoma Arisaematis (processed), 20 grams of Herba Lycopi, 20 grams of rhizoma alismatis,
Add the ethanol of total 10 times of volumes of medicinal material weight, then ultrasonic when immersion 24 is small, ultrasonic extraction 2 times, every time 2 it is small when, merge
Extracting solution, concentration, macroporous resin column on concentrate, successively with water, the ethanol elution of 20% ethanol~60%, collects 40~50% second
Alcohol eluen, concentration, obtains extract；
(2) take 6 grams of centipede in parts by weight, with petroleum ether continuous circumfluence extraction 2 times, every time 2 it is small when, obtain petroleum ether extraction
Liquid, is concentrated and dried, obtains Scolopendra extract；
(3) extract for taking step (1) to obtain, the Scolopendra extract that step (2) obtains, then add diluent lactose or
Cornstarch is uniformly mixed, whole grain, dry, and granule is made.(lot number：002)
Implementation column 3
1. a kind of Chinese medicine composition with anti-cancer of the brain activity, it is made of the bulk pharmaceutical chemicals of following parts by weight：RHIZOMA TYPHONII PREPARATA
30 grams of son, 45 grams of edible tulip, 9 grams of centipede, 30 grams of Rhizoma Arisaematis (processed), 30 grams of Herba Lycopi, 30 grams of rhizoma alismatis.
2. the preparation method of the Chinese medicine composition of the present invention with anti-cancer of the brain activity, it comprises the following steps：
(1) 30 grams of processed rhizoma typhonii or radix aconiti corean is taken in parts by weight, 45 grams of edible tulip, 30 grams of Rhizoma Arisaematis (processed), 30 grams of Herba Lycopi, 30 grams of rhizoma alismatis,
Add the ethanol of total 8 times of volumes of medicinal material weight, then ultrasonic when immersion 48 is small, refluxing extraction 2 times, every time 3 it is small when, merge
Extracting solution, concentration, macroporous resin column on concentrate, successively with water, the ethanol elution of 20% ethanol~60%, collects 40~50% second
Alcohol eluen, concentration, obtains extract；
(2) take 6 grams of centipede in parts by weight, with petroleum ether continuous circumfluence extraction 3 times, every time 1 it is small when, obtain petroleum ether extraction
Liquid, is concentrated and dried, obtains Scolopendra extract；
(3) extract for taking step (1) to obtain, the Scolopendra extract that step (2) obtains, then and lactose, cornstarch
Mixing with whole grain is then encapsulated that capsule is made.(lot number：003)
4 active Immunotherapy of Cancer Induced of implementation column
(1) to the inhibitory action of brain cancer cell strain GBM8401 in nude mouse
Experimental method：Brain cancer cell strain GBM8401 cell suspensions are prepared, adjust cell concentration, often armpit notch graft before the mouse right side
Kind FTC-133 cells 5 × 106A/0.2ml, altogether be inoculated with 24, be randomly divided into treatment group (embodiment of the present invention 1 provide have
The Chinese medicine composition of anti-cancer of the brain activity, 001. gastric infusion of lot number, 2g/kg) and blank group (waiting capacity physiological saline), every group 12
Only, 24 it is small when after, start medication, once a day, continuous 24 days, after drug withdrawal, put to death animal, observation tumour growth situation simultaneously take
Tumor tissue is weighed, and specific experiment the results are shown in Table 1.
Inhibitory action of the table 1 to brain cancer cell strain GBM8401 cells in nude mouse
(2) external anti-cancer of the brain active testing
Cell line：Brain cancer cell GBM8401；96 orifice plates are purchased from Costar companies；RMPI1640 culture mediums are public purchased from Gibco
Department；DMEM culture mediums are purchased from Gibco companies；Newborn bovine serum is purchased from Hangzhou Chinese holly company；Tetrazolium bromide (MTT) and DMSO are purchased from
Amresco companies.
Laboratory apparatus is as follows used in following embodiments：
Laboratory apparatus：Superclean bench (Suzhou purifies model SW-CJ-IFD), CO2Incubator (SANYO models：XD-
101), microplate reader BIO-RAD (Model NO.550Serial NO.16971).
Experimental method is as follows used in following embodiments：
Experimental method：The Chinese medicine composition that the embodiment of the present invention 1 is prepared using improvement mtt assay carries out external anti-human
Brain cancer cell is tested：Brain cancer cell GBM8401 is digested with pancreatin, is counted, concentration is made as 5 × 104The cell of a/ml
Suspension.100 μ l cell suspensions will be added in 96 orifice plates per hole (per hole 5 × 103A cell), 37 DEG C are subsequently placed in, 5%CO2Culture
When culture 24 is small in case；Medicine is diluted to required different gradient concentrations with non-fully culture medium, and it is corresponding that 100 μ L are added per hole
Pastille culture medium, while set up negative control group, vehicle control group and positive controls, then 96 orifice plates are placed in 37 DEG C, 5%
CO2When culture 72 is small in incubator.Then per hole add 20 μ L MTT (5mg/ml), continue culture 4 it is small when, terminate culture, abandon
Culture medium is removed, 150 μ L DMSO dissolvings are added per hole, shaking table gently mixes for 10 minutes.Under λ=4570nm, two wavelength of 620nm
Absorbance, that is, OD values per hole are detected with microplate reader, the OD values using the average value of each multiple holes as this group of cell, calculate each medicine
Inhibiting rate.
Experimental result shows the Chinese medicine composition that the embodiment of the present invention 1 is prepared, and has to human brain cancer cells GBM8401
Stronger inhibitory action, each dosage group present preferable dose-effect relationship.Experimental result is as shown in table 2.
Inhibitory action result of the table 2 to human brain cancer cells GBM8401
By more than test result indicates that：Chinese medicine composition provided by the invention with anti-cancer of the brain activity is to people cancer of the brain nude mice
Transplanting in vivo has obvious inhibitory action, while the external increment that also can substantially suppress brain cancer cell, shows offer of the present invention
The Chinese medicine composition with anti-cancer of the brain activity be respectively provided with the very strong anti-cancer of the brain in vivo and in vitro, can be used for cancer of the brain disease
The treatment of disease.Implementation column 5 has experiment of the Chinese medicine composition of anti-cancer of the brain activity to immunoregulation effect
(1) to the influence of mouse IgA, IgG, IgM content
Experimental method：Take ICR small white mouses 30,18~22g of weight, be randomly divided into Normal group, lotus knurl control group and
Treatment group, traditional vaccination brain cancer cell, after inoculation 24 it is small when, what treatment group gavaged that the embodiment of the present invention 1 is prepared has anti-
Chinese medicine composition (lot number 001) 0.3ml/ of cancer of the brain activity only (2g/kg), take a blood sample within the 15th day by small white mouse eyeball.Measure IgA,
IgG, IgM content, specific experiment the results are shown in Table 3.
Influence (mg/dl of the table 3 to mouse IgA, IgG, IgM content)
*P<0.01 (compared with Normal group) * * P<0.05 (compared with lotus knurl control group)
By with upper table 3 test result indicates that：The mouse of the cancer of the brain is inoculated with, Immunoglobulin IgA, IgG, IgM content are obvious
Less than normal control mice；The present invention has IgA, IgG, IgM that the Chinese medicine composition of anti-cancer of the brain activity can significantly improve mouse
Content, shows the Chinese medicine composition provided by the invention with anti-cancer of the brain activity with stronger immunologic enhancement, Ke Yiti
The high tumor patient immune function of itself, so as to be conducive to improve Quality of Life of Tumor Patients and promote itself antitumor action.
(2) the raising effect induced to tumor necrosis factor (INF)
Experimental method：Wistar big white mouse 20 are taken, are randomly divided into control group and treatment group, every animal is injected intraperitoneally
10mg BCG vaccines, the 80 μ gLPS of intraperitoneal injection in the 15th day, take a blood sample after 90 minutes.Treatment group gavages the embodiment of the present invention 1 with anti-
Only (lot number 001,2g/kg), continuous 14 days, control group such as gavaged at the capacity physiological saline to the Chinese medicine composition 2ml/ of cancer of the brain activity.
Test serum is taken, is diluted to various concentrations；Target cell L929 10% calf serum, RPM1-1640 nutrient solution tune
Whole is 2 × 106/ ml cells, add in 96 porocyte culture plates；100 μ l/ holes, then add the test serum 100 of different extension rates
μ l/ holes；37 DEG C are put, 5%CO2When culture 18 is small in incubator.Result judgement：With the maximum dilution multiple of 50% target cell death
For INF potency.Concrete outcome is shown in Table 4.
The facilitation that table 4 induces rat INF
*P<0.001 (compared with Normal group)
By table 4 test result indicates that：Chinese medicine composition provided by the invention with anti-cancer of the brain activity can be obviously promoted
INF's induces, (P<0.001), illustrate that the Chinese medicine composition provided by the invention with anti-cancer of the brain activity can pass through induced tumors
Necrosin INF is antitumor to achieve the purpose that.
Test result indicates that, the Chinese medicine composition provided by the invention with anti-cancer of the brain activity is to tumor patient more than
Immune function is acted on raising, and can promote the generation of tumor necrosis factor (INF), can strengthen the antitumor work(of patient itself
Energy.
The present invention carries out proportioning prescription, medication is rigorous, passes through the above according to traditional Chinese medical theory using the theory of diagnosis and treatment
Test result indicates that the Chinese medicine composition provided by the invention with anti-cancer of the brain activity is with work(such as anticancer removing toxic substances, eleminating phlegm and freeing channels
Effect, has the growth and transferance for suppressing cancer of the brain tumour, and without obvious toxic-side effects, illustrates provided by the invention there is anti-brain
The Chinese medicine composition of cancer activity can be used for the novel drugs for preparing the anti-cancer of the brain.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of Chinese medicine composition with anti-cancer of the brain activity, it is characterised in that it is made of the bulk pharmaceutical chemicals of following parts by weight：
10~30 parts of processed rhizoma typhonii or radix aconiti corean, 15~45 parts of edible tulip, 3~9 parts of centipede, 10~30 parts of Rhizoma Arisaematis (processed), 10~30 parts of Herba Lycopi, pool
Rush down 10~30 parts.
2. the Chinese medicine composition according to claim 1 with anti-cancer of the brain activity, it is characterised in that it is by following parts by weight
Several bulk pharmaceutical chemicals are made：10~20 parts of processed rhizoma typhonii or radix aconiti corean, 15~30 parts of edible tulip, 3~6 parts of centipede, 10~20 parts of Rhizoma Arisaematis (processed), Herba Lycopi
10~20 parts, 10~20 parts of rhizoma alismatis.
3. the Chinese medicine composition according to claim 2 with anti-cancer of the brain activity, it is characterised in that it is by following parts by weight
Several bulk pharmaceutical chemicals are made：10 parts of processed rhizoma typhonii or radix aconiti corean, 15 parts of edible tulip, 3 parts of centipede, 10 parts of Rhizoma Arisaematis (processed), 10 parts of Herba Lycopi, 10 parts of rhizoma alismatis.
4. the Chinese medicine composition according to any one of claims 1 to 3 with anti-cancer of the brain activity, it is characterised in that will be each
The extract and pharmaceutically acceptable carrier of bulk pharmaceutical chemicals are prepared into the medicine of oral liquid, granule, capsule or Tabules.
5. claims 1 to 3 any one of them has the preparation method of the Chinese medicine composition of anti-cancer of the brain activity, it is characterised in that
Comprise the following steps：
(1) processed rhizoma typhonii or radix aconiti corean, edible tulip, Rhizoma Arisaematis (processed), Herba Lycopi and rhizoma alismatis are taken by claims 1 to 3 any one of them parts by weight,
The ethanol of total 6~15 times of volumes of medicinal material weight is added, then ultrasonic when immersion 24~48 is small, reflux or continuous backflow carry
Take 2~3 times, every time 1~3 it is small when, merge extracting solution, concentration, macroporous resin column on concentrate, successively with water, 20% ethanol~
60% ethanol elution, collects 40~50% ethanol eluates, concentration, obtains extract；
(2) centipede is taken by claims 1 to 3 any one of them parts by weight, with petroleum ether continuous circumfluence extraction 2~3 times, often
It is secondary 1~3 it is small when, obtain petroleum ether extract, be concentrated and dried, obtain Scolopendra extract；
(3) it is prepared by the extract for taking step (1) to obtain, the Scolopendra extract and pharmaceutically acceptable carrier that step (2) obtains
Into the medicine of oral liquid, granule, capsule or Tabules.
6. the preparation method of the Chinese medicine composition with anti-cancer of the brain activity described in claim 5, it is characterised in that step (1)
The macroreticular resin is D101 type resins.
7. claims 1 to 3 any one of them has the Chinese medicine composition of anti-cancer of the brain activity in anti-cancer of the brain medicine is prepared
Using.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1246358A (en) * | 1999-08-10 | 2000-03-08 | 辛长山 | Medical capsule for treating cerebroma and its preparing process |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1246358A (en) * | 1999-08-10 | 2000-03-08 | 辛长山 | Medical capsule for treating cerebroma and its preparing process |
Non-Patent Citations (1)
| Title |
|---|
| 周仲瑛治疗肿瘤的临证思路探析;霍介格等;《上海中医药杂志》;20070131;第41卷(第1期);第6页左栏"5.1脑瘤" * |
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