CN101341852B - Cross breeding method for black cypress and southern cypress - Google Patents
Cross breeding method for black cypress and southern cypress Download PDFInfo
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- CN101341852B CN101341852B CN 200710025662 CN200710025662A CN101341852B CN 101341852 B CN101341852 B CN 101341852B CN 200710025662 CN200710025662 CN 200710025662 CN 200710025662 A CN200710025662 A CN 200710025662A CN 101341852 B CN101341852 B CN 101341852B
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Abstract
The invention relates to a taxodi ummucronatum(male) * swampcypress(female) cross breeding method; to solve the technical difficulties that the female flowers of a Nanjing taxodi ummucronatum tree are mature too early and easy to suffer from freezing injury, and the male flowers grow slowly and cannot conduct natural pollination, so the tree cannot bear fruit and multiply offspring naturally, theinvention adopts a taxodi ummucronatum(male) * mpcypress(female) elss combined artificial pollination cross breeding method, to obtain cross breeding fruit, seeds and seedlings. From the growth of the cross breeding seedlings, the tree not only has the evergreenness of paternal taxodi ummucronatum, but also has the dry and straight advantages of paternal swampcypress, and the height and stem of the cross breeding seedlings obviously grow faster than those of parent seedlings, which shows obvious cross breeding advantages; therefore, the cross breeding advantages are obvious, and good development potential and practical promotion value are expected.
Description
Technical field:
The tree breeding technical field is specifically related to a kind of black China fir (♀) * swamp cypress
Cross breeding method.
Background technology:
Institute of Botany has been engaged in the crossbreeding research work of Taxodium seeds always since the seventies in last century, at swamp cypress (♀) * Chi Shan
Swamp cypress (♀) * Mo Shan
And in the back cross breeding of offspring and black China fir, having obtained with middle mountain China fir 302 national kinds is a collection of forest breeding of representative, has brought into play good economic benefits.Carried out the exploratory development of the new hybrid combination possibilities of Taxodium trees in recent years again, at present domestic as yet not to black China fir (♀) * swamp cypress
Hybrid combination is effectively studied, its difficulty is: the big tree female flower of the black China fir in Nanjing is precocious and be subject to freeze injury, the male flower hypoevolutism, can not natural loose powder, and the swamp cypress bearing tree florescence is all than the slow 10-15 of black China fir days, therefore both florescence differences are obvious, and the crossbreeding technology difficulty of this hybrid combination is difficult to capture always.The present invention is precocious and be subject to freeze injury in order to solve the big tree female flower of black China fir, and the male flower hypoevolutism can not natural loose powder, thereby can not be solid naturally and the technical barrier that produces offspring, and has adopted black China fir (♀) * swamp cypress
New hybrid combination artificial pollination cross method has obtained hybridization fruit, seed and seedling.From hybrid seedling growing way, both had the evergreenness of maternal black China fir, have the perfectly straight advantage of male parent swamp cypress dry type again, and the height of hybrid seedling, footpath growth are also obviously fast than the parent, show tangible hybrid vigour.
Summary of the invention:
The objective of the invention is at black China fir (♀) * swamp cypress
New hybrid combination artificial pollination cross method.
Another object of the present invention is to obtain black China fir (♀) * swamp cypress
New hybrid combination artificial pollination hybridization fruit, seed and seedling.
Black China fir of the present invention (♀) * swamp cypress
Cross breeding method is characterized in that described black China fir (♀) * swamp cypress
Following method crossbreeding: expanded and the displaing yellow male inflorescence of loose powder not gathering 10-15 day in March from the swamp cypress select tree of 30 years age of trees, put indoor the stand on blank sheet of paper, rapped inflorescence with penholder through 1-2 days after the drying, pollen is scattered on the paper, pour in the glass vial with being about to pollen, pollen amount is no more than 1/3 of the vial degree of depth, the a small amount of absorbent cotton of plug in the pipe, the mouth of pipe ties with double gauze, put into drier and be built in 5-10 ℃ of refrigerator preservation 20-30 days, transfer to long preservation under-10 to-17 ℃ of refrigerator conditions then.Test the 6 years living grafting tree China ink China firs in nursery as elite stand with Institute of Botany in addition, the annual female cone branch that will physically well develop before female flower is open is inserted in 12 * 16cm glassine paper pocket, base portion is tightened with the metal flexible cord, and spray is inclined upwardly, 1-10 day in March when treating that female flower is in full bloom, glass tube tip hole with rear sleeve capsule ball penetrates in the bag, pinch the capsule ball with have gentle hands, swamp cypress pollen is sent forth to female cone, extract glass-tube then and use finger to twist the little wound hole of pressed glass paper bag, to 26-28 day in March separating bag, at the hybridization fruit and take out seed of gathering 1-10 day in the October in the same year, and be seeded in Institute of Botany test nursery 10-20 day March in next year, the hybrid seedling of current growth had both had half evergreenness of maternal black China fir, has the perfectly straight advantage of male parent swamp cypress dry type again, its height, the footpath amount of growth has begun to show good hybrid vigour all apparently higher than the parent.
Effect of the present invention is Mo Shan (♀) * swamp cypress
New hybrid combination is tested in 2004 first and is succeedd, 3 of results hybridization fruits, and 54 in seed, the impurity removal seedling was 9 in 2005, kept 3 strain hybrid seedlings: be numbered 405,406 and 407.Results hybridization fruit was 4 in 2005,87 in seed, and impurity removal seedling 5 strains in 2006 keep 3 strain hybrid seedlings: be numbered 501,502 and 503.Results hybridization fruit was 1 in 2006,18 in seed, and impurity removal seedling 2 strains in 2007 keep 1 strain of hybrid seedling: be numbered 601.
From hybrid seedling growing way, both had the evergreenness of maternal black China fir, have the perfectly straight advantage of male parent swamp cypress dry type again, and the height of hybrid seedling, footpath growth are also obviously fast than the parent, show tangible hybrid vigour, therefore black China fir (♀) * swamp cypress
Filial generation has bright development prospect and practical reference value.
Embodiment: one
1. swamp cypress pollen is gathered and storage technique
On March 10th, 2003, gather from the swamp cypress select tree of 30 years age of trees and to have expanded and the displaing yellow male inflorescence of loose powder not, put indoor the stand on blank sheet of paper, rapped inflorescence with penholder through 1 day after the drying, pollen is scattered on the paper, pour in the glass vial with being about to pollen, pollen amount is no more than 1/3 of the vial degree of depth, the a small amount of absorbent cotton of plug in the pipe, the mouth of pipe ties with double gauze, put into drier and be built in 5 ℃ of refrigerators preservations 20 days, transfer to long preservation under-10 ℃ of refrigerator conditions then, use for spring in 2004 hybridization.
2. black China fir elite stand is cultivated and hybridization technique
On February 30th, 2004 with 6 years black China firs of livings grafting tree in Institute of Botany test nursery as elite stand, the female cone branch that will physically well develop before female flower is open is inserted in 12 * 16cm glassine paper pocket, base portion is tightened with the metal flexible cord, and spray is inclined upwardly, March 3 when treating that female flower is in full bloom, glass tube tip hole with rear sleeve capsule ball penetrates in the bag, pinch the capsule ball with have gentle hands, swamp cypress pollen is sent forth to female cone, extracted glass-tube then and with the little wound hole of finger twisting pressed glass paper bag.Separated bag to March 28, gathered on October 2nd, 2004 and hybridize 3 of fruits, and take out 54 of hybrid seeds.
3. black China fir (♀) * swamp cypress
The cultivation of hybridization seedling
On March 1st, 2005 is with black China fir (♀) * swamp cypress
The hybrid seed that hybridization is cultivated is seeded in nursery in the institute, and 9 of impurity removal seedlings keep 3 strain hybrid seedlings: be numbered 405,406 and 407.
Embodiment: two
1. swamp cypress pollen is gathered and storage technique
On March 15th, 2004, gather from the swamp cypress select tree of 30 years age of trees and to have expanded and the displaing yellow male inflorescence of loose powder not, put indoor the stand on blank sheet of paper, rapped inflorescence with penholder through 1.5 days after the drying, pollen is scattered on the paper, pour in the glass vial with being about to pollen, pollen amount is no more than 1/3 of the vial degree of depth, the a small amount of absorbent cotton of plug in the pipe, the mouth of pipe ties with double gauze, put into drier and be built in 7 ℃ of refrigerators preservations 25 days, transfer to long preservation under-13 ℃ of refrigerator conditions then, use for spring in 2005 hybridization.
2. black China fir elite stand is cultivated and hybridization technique
On March 5th, 2005 with 6 years black China firs of livings grafting tree in Institute of Botany test nursery as elite stand, the female cone branch that will physically well develop before female flower is open is inserted in 12 * 16cm glassine paper pocket, base portion is tightened with the metal flexible cord, and spray is inclined upwardly, March 5 when treating that female flower is in full bloom, glass tube tip hole with rear sleeve capsule ball penetrates in the bag, pinch the capsule ball with have gentle hands, swamp cypress pollen is sent forth to female cone, extracted glass-tube then and with the little wound hole of finger twisting pressed glass paper bag.Separated bag to March 29, gathered on October 8th, 2005 and hybridize 4 of fruits, and take out 87 of hybrid seeds.
3. black China fir (♀) * swamp cypress
The cultivation of hybridization seedling
On March 5th, 2006 is with black China fir (♀) * swamp cypress
The hybrid seed that hybridization is cultivated is seeded in nursery in the institute, and 5 strains of impurity removal seedling keep 3 strain hybrid seedlings: be numbered 501,502 and 503.
Embodiment: three
1. swamp cypress pollen is gathered and storage technique
On March 13rd, 2005, gather from the swamp cypress select tree of 30 years age of trees and to have expanded and the displaing yellow male inflorescence of loose powder not, put indoor the stand on blank sheet of paper, rapped inflorescence with penholder through 2 days after the drying, pollen is scattered on the paper, pour in the glass vial with being about to pollen, pollen amount is no more than 1/3 of the vial degree of depth, the a small amount of absorbent cotton of plug in the pipe, the mouth of pipe ties with double gauze, put into drier and be built in 10 ℃ of refrigerators preservations 30 days, transfer to long preservation under-17 ℃ of refrigerator conditions then, use for spring in 2006 hybridization.
2. black China fir elite stand is cultivated and hybridization technique
On March 10th, 2006 with 6 years black China firs of livings grafting tree in Institute of Botany test nursery as elite stand, the female cone branch that will physically well develop before female flower is open is inserted in 12 * 16cm glassine paper pocket, base portion is tightened with the metal flexible cord, and spray is inclined upwardly, March 7 when treating that female flower is in full bloom, glass tube tip hole with rear sleeve capsule ball penetrates in the bag, pinch the capsule ball with have gentle hands, swamp cypress pollen is sent forth to female cone, extracted glass-tube then and with the little wound hole of finger twisting pressed glass paper bag.Separated bag to March 30, gathered on October 10th, 2006 and hybridize 1 of fruit, and take out 18 of hybrid seeds.
3. black China fir (♀) * swamp cypress
The cultivation of hybridization seedling
On March 10th, 2007 is with black China fir (♀) * swamp cypress
The hybrid seed that hybridization is cultivated is seeded in nursery in the institute, and 2 strains of impurity removal seedling keep 1 strain of hybrid seedling: be numbered 601.
Claims (2)
1. black China fir ♀ * swamp cypress
Cross breeding method is characterized in that described black China fir ♀ * swamp cypress
Following method crossbreeding:
Expanded and the displaing yellow male inflorescence of loose powder not gathering 10-15 day in March from the swamp cypress select tree of 30 years age of trees, put indoor the stand on blank sheet of paper, rapped inflorescence with penholder through 1-2 days after the drying, pollen is scattered on the paper, pour in the glass vial with being about to pollen, pollen amount is no more than 1/3 of the vial degree of depth, the a small amount of absorbent cotton of plug in the pipe, the mouth of pipe ties with double gauze, put into drier and be built in 5-10 ℃ of refrigerator preservation 20-30 days, transfer to long preservation under-10 to-17 ℃ of refrigerator conditions then, test the 6 years living grafting tree Taxodium mucronatums in nursery as elite stand with Institute of Botany in addition, the annual female cone branch that will physically well develop before female flower is open is inserted in 12 * 16cm glassine paper pocket, base portion is tightened with the metal flexible cord, and spray is inclined upwardly, 1-10 day in March when treating that female flower is in full bloom, glass tube tip hole with rear sleeve capsule ball penetrates in the bag, pinch the capsule ball with have gentle hands, swamp cypress pollen is sent forth to female to be taken, extract glass-tube then and, separate bag in 26-28 day in March with the little wound hole of pointing twisting pressed glass paper bag.
2. black China fir ♀ * swamp cypress according to claim 1
Cross breeding method is characterized by Mo Shan ♀ * swamp cypress
The hybridization tree is gathered in early October and hybridizes fruit and take out seed, to being seeded in Institute of Botany test nursery 10-20 day March in next year, according to newly going out the sapling observation on Growth, it had both possessed the evergreenness of maternal black China fir, have the perfectly straight characteristics of male parent swamp cypress dry type again, its height, footpath amount of growth are all apparently higher than the parent.
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| CN 200710025662 CN101341852B (en) | 2007-08-10 | 2007-08-10 | Cross breeding method for black cypress and southern cypress |
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| CN 200710025662 CN101341852B (en) | 2007-08-10 | 2007-08-10 | Cross breeding method for black cypress and southern cypress |
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| CN101341852A CN101341852A (en) | 2009-01-14 |
| CN101341852B true CN101341852B (en) | 2010-12-08 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687653A (en) * | 2011-08-12 | 2012-09-26 | 何小弟 | Cultivation method for increasing summer transplanting survival rate of engineering seedlings of north American taxodium distichum |
| CN106258704A (en) * | 2015-06-01 | 2017-01-04 | 正安县顶箐方竹笋有限公司 | A kind of method for culturing seedlings of bald cypress |
| CN108781850A (en) * | 2017-05-05 | 2018-11-13 | 江苏溯源农业科技发展有限公司 | A kind of Salt-Tolerance Identification method of the black China fir of hybridization |
| CN108781950A (en) * | 2017-05-05 | 2018-11-13 | 江苏溯源农业科技发展有限公司 | A method of popularizing planting is carried out to the black China fir saline-alkali tolerant new varieties of hybridization |
| CN109418153B (en) * | 2017-08-29 | 2021-07-20 | 江苏省中国科学院植物研究所 | Cross breeding method of black cypress and male cypress |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4102079A (en) * | 1976-07-05 | 1978-07-25 | Setsuo Kataoka | Method of breeding new varieties of the first filial generation plant |
| CN1287776A (en) * | 2000-10-24 | 2001-03-21 | 王恭祎 | Method for breeding new varieties of poplar |
-
2007
- 2007-08-10 CN CN 200710025662 patent/CN101341852B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4102079A (en) * | 1976-07-05 | 1978-07-25 | Setsuo Kataoka | Method of breeding new varieties of the first filial generation plant |
| CN1287776A (en) * | 2000-10-24 | 2001-03-21 | 王恭祎 | Method for breeding new varieties of poplar |
Non-Patent Citations (6)
| Title |
|---|
| 李涵等.落羽杉属树种及其杂交后代亲缘关系的RAPD分析.林业科学43 2.2007,43(2),48-51. |
| 李涵等.落羽杉属树种及其杂交后代亲缘关系的RAPD分析.林业科学43 2.2007,43(2),48-51. * |
| 殷云龙等.中山杉302回交一代的早期选育.植物资源与环境学报12 2.2003,12(2),22-27. |
| 殷云龙等.中山杉302回交一代的早期选育.植物资源与环境学报12 2.2003,12(2),22-27. * |
| 陈永辉等.落羽杉中山杉系列新品种选育初报.江苏林业科技33 4.2006,33(4),1-5. |
| 陈永辉等.落羽杉中山杉系列新品种选育初报.江苏林业科技33 4.2006,33(4),1-5. * |
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Application publication date: 20090114 Assignee: Jiangsu traceability agricultural science and Technology Development Co Ltd Assignor: Institute of Botany, Jiangsu Chinese Academy of Sciences Contract record no.: 2013320000693 Denomination of invention: Cross breeding method for black cypress and southern cypress Granted publication date: 20101208 License type: Exclusive License Record date: 20130916 |
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