CN101302473B - 实时pcr系统 - Google Patents

实时pcr系统 Download PDF

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Publication number
CN101302473B
CN101302473B CN2008100961874A CN200810096187A CN101302473B CN 101302473 B CN101302473 B CN 101302473B CN 2008100961874 A CN2008100961874 A CN 2008100961874A CN 200810096187 A CN200810096187 A CN 200810096187A CN 101302473 B CN101302473 B CN 101302473B
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CN
China
Prior art keywords
temperature
real
conversion zone
thermal source
control
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2008100961874A
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English (en)
Chinese (zh)
Other versions
CN101302473A (zh
Inventor
森胁俊贵
世取山翼
濑川雄司
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Sony Corp
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Sony Corp
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Filing date
Publication date
Application filed by Sony Corp filed Critical Sony Corp
Publication of CN101302473A publication Critical patent/CN101302473A/zh
Application granted granted Critical
Publication of CN101302473B publication Critical patent/CN101302473B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K7/00—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements
    • G01K7/01—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using semiconducting elements having PN junctions
    • G01K7/015—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using semiconducting elements having PN junctions using microstructures, e.g. made of silicon
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00783—Laminate assemblies, i.e. the reactor comprising a stack of plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00851—Additional features
    • B01J2219/00853—Employing electrode arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00781—Aspects relating to microreactors
    • B01J2219/00873—Heat exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008100961874A 2007-05-11 2008-05-09 实时pcr系统 Expired - Fee Related CN101302473B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-126344 2007-05-11
JP2007126344A JP5205802B2 (ja) 2007-05-11 2007-05-11 リアルタイムｐｃｒ装置

Publications (2)

Publication Number Publication Date
CN101302473A CN101302473A (zh) 2008-11-12
CN101302473B true CN101302473B (zh) 2013-10-16

Family

ID=39619177

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008100961874A Expired - Fee Related CN101302473B (zh) 2007-05-11 2008-05-09 实时pcr系统

Country Status (4)

Country Link
US (1) US8017380B2 (enExample)
EP (1) EP1994980A1 (enExample)
JP (1) JP5205802B2 (enExample)
CN (1) CN101302473B (enExample)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9475051B2 (en) 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
JP4458133B2 (ja) * 2007-02-27 2010-04-28 ソニー株式会社 核酸増幅装置
JP5045268B2 (ja) 2007-06-28 2012-10-10 ソニー株式会社 反応処理装置
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムｐｃｒ装置
JP5310373B2 (ja) * 2009-05-14 2013-10-09 ソニー株式会社 光学的検出装置
US8419273B2 (en) * 2010-05-03 2013-04-16 Sharp Kabushiki Kaisha Array element for temperature sensor array circuit, temperature sensor array circuit utilizing such array element, and AM-EWOD device including such a temperature sensor array circuit
JP2012118055A (ja) * 2010-11-12 2012-06-21 Sony Corp 反応処理装置及び反応処理方法
KR101853882B1 (ko) * 2011-01-10 2018-05-02 삼성전자주식회사 생체물질 검사장치 및 그 제어방법
US9988668B2 (en) * 2011-06-23 2018-06-05 Anitoa Systems, Llc Apparatus for amplification of nucleic acids
CN103635568B (zh) 2011-06-24 2016-04-27 株式会社日立高新技术 核酸扩增装置和核酸分析装置
CN102533544B (zh) * 2011-12-31 2013-07-10 中山大学达安基因股份有限公司 一种电化学基因检测系统
CN102586098B (zh) * 2012-02-29 2013-11-20 北京工业大学 一种面向空间的微体积单位的实时荧光pcr工作系统
JP6298048B2 (ja) * 2012-06-28 2018-03-20 フルオレセントリック，インコーポレイテッド 化学的インジケータデバイス
TWI458828B (zh) * 2012-07-18 2014-11-01 Univ Nat Pingtung Sci & Tech Droplet type high throughput thermal cycling system
AU2013202793B2 (en) * 2012-07-31 2014-09-18 Gen-Probe Incorporated System, method and apparatus for automated incubation
JP6087138B2 (ja) 2012-12-28 2017-03-01 株式会社日立ハイテクノロジーズ 遺伝子検査装置、遺伝子検査方法及びプログラム
KR101696259B1 (ko) 2014-07-23 2017-01-13 나노바이오시스 주식회사 멀티플렉스 ｐｃｒ 칩 및 이를 포함하는 멀티플렉스 ｐｃｒ 장치
CN106328659B (zh) * 2016-08-19 2019-06-14 京东方科技集团股份有限公司 阵列基板及其制备方法、显示面板
CN107058090A (zh) * 2017-04-27 2017-08-18 滨江华康（北京）生物科技有限公司 一种实时荧光定量pcr基因扩增检测仪
CN107460121A (zh) * 2017-07-22 2017-12-12 新疆昆泰锐生物技术有限公司 一种pcr反应体系配制及进样装置及全自动pcr仪
CN107460108A (zh) * 2017-07-23 2017-12-12 新疆昆泰锐生物技术有限公司 一种连续进样的pcr反应体系配制及进样装置及pcr仪
CN107653187A (zh) * 2017-11-07 2018-02-02 安图实验仪器(郑州)有限公司 随机pcr系统
JP7157658B2 (ja) * 2018-12-27 2022-10-20 株式会社日立ハイテク 自動分析装置
WO2021210298A1 (ja) 2020-04-14 2021-10-21 Ｐｈｃホールディングス株式会社 核酸検査装置および核酸検査方法
CN118401676A (zh) 2021-09-23 2024-07-26 N6科技公司 样本分析的方法和系统
CN114672405A (zh) * 2022-03-21 2022-06-28 诺美纳瑞(广州)医疗技术有限公司 一种pcr扩增检测集成系统及其pcr反应控制方法
USD1069156S1 (en) 2023-04-10 2025-04-01 Becton, Dickinson And Company Dispensing device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1687391A (zh) * 2005-04-21 2005-10-26 中国科学院上海微系统与信息技术研究所 用于驱动聚合酶链式反应微芯片阵列的温度控制方法及装置
US20060073491A1 (en) * 2004-05-28 2006-04-06 Victor Joseph Apparatus and method for multiplex analysis

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2129787A1 (en) * 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
AU5887000A (en) * 1999-06-22 2001-01-09 Tecan Trading Ag Devices and methods for the performance of miniaturized in vitro amplification assays
JP4071907B2 (ja) * 1999-11-29 2008-04-02 オリンパス株式会社 自動核酸検査装置
US7727479B2 (en) * 2000-09-29 2010-06-01 Applied Biosystems, Llc Device for the carrying out of chemical or biological reactions
WO2002081729A2 (en) * 2001-04-06 2002-10-17 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6762049B2 (en) * 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
US6504226B1 (en) 2001-12-20 2003-01-07 Stmicroelectronics, Inc. Thin-film transistor used as heating element for microreaction chamber
US20030116552A1 (en) 2001-12-20 2003-06-26 Stmicroelectronics Inc. Heating element for microfluidic and micromechanical applications
KR100459896B1 (ko) 2002-03-06 2004-12-04 삼성전자주식회사 Ｐｃｒ 칩을 구동하기 위한 온도 제어 방법 및 그 장치
AU2003243281A1 (en) * 2002-05-17 2003-12-02 Applera Corporation Optical instrument includung excitation source
KR101216828B1 (ko) * 2002-12-30 2013-01-04 더 리전트 오브 더 유니버시티 오브 캘리포니아 병원균 검출과 분석을 위한 방법과 기구
EP1541237A3 (en) 2003-12-10 2006-02-01 Samsung Electronics Co., Ltd. Polymerase chain reaction (pcr) module and multiple pcr system using the same
JP2006003222A (ja) * 2004-06-17 2006-01-05 Daikin Ind Ltd Ｄｎａ検出装置及びｄｎａ検出用電極
US20070212681A1 (en) * 2004-08-30 2007-09-13 Benjamin Shapiro Cell canaries for biochemical pathogen detection
JP4771043B2 (ja) * 2004-09-06 2011-09-14 日本電気株式会社 薄膜半導体素子及びその駆動回路並びにそれらを用いた装置
WO2007034374A2 (en) 2005-09-23 2007-03-29 Koninklijke Philips Electronics N.V. A micro-fluidic device based upon active matrix principles
US8124033B2 (en) * 2006-02-17 2012-02-28 Agency, Science, Technology and Research Apparatus for regulating the temperature of a biological and/or chemical sample and method of using the same
US20100163414A1 (en) * 2006-03-21 2010-07-01 Koninklijke Philips Electronics N.V. Microelectronic device with field electrodes
US7881594B2 (en) * 2007-12-27 2011-02-01 Stmicroeletronics, Inc. Heating system and method for microfluidic and micromechanical applications

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060073491A1 (en) * 2004-05-28 2006-04-06 Victor Joseph Apparatus and method for multiplex analysis
CN1687391A (zh) * 2005-04-21 2005-10-26 中国科学院上海微系统与信息技术研究所 用于驱动聚合酶链式反应微芯片阵列的温度控制方法及装置

Also Published As

Publication number Publication date
JP2008278810A (ja) 2008-11-20
JP5205802B2 (ja) 2013-06-05
US8017380B2 (en) 2011-09-13
EP1994980A1 (en) 2008-11-26
US20080280350A1 (en) 2008-11-13
CN101302473A (zh) 2008-11-12

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