CN101297632A - Medium prescription for production of fungus-treated tobacco rooted seedlings by floating nursery - Google Patents
Medium prescription for production of fungus-treated tobacco rooted seedlings by floating nursery Download PDFInfo
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- CN101297632A CN101297632A CNA2008100698272A CN200810069827A CN101297632A CN 101297632 A CN101297632 A CN 101297632A CN A2008100698272 A CNA2008100698272 A CN A2008100698272A CN 200810069827 A CN200810069827 A CN 200810069827A CN 101297632 A CN101297632 A CN 101297632A
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Abstract
The invention relates to a special matrix formula suitable for culturing mycorrhizal tobacco seedling in the condition of flue-cured tobacco seedling by floating system; all the constituents and the volume thereof are as follows: 60 percent of peat, 20 percent of perlite and 20 percent of vermiculite. The formula can improve the comprehensive quality of the tobacco seedling, cultures sound seedlings and has the advantages of wide material sources, easy acquisition, easy matrix preparation, simple production, simple inoculating operation, little fungicide dosage and easy promotion, etc. The culture medium, the inoculating technique and the corresponding Glomus intraradices Smith and Schenck are combined together, infection rate of mycorrhiza is up to over 40 percent and the effect of the mycorrhiza is good.
Description
Technical field
The present invention relates to modern agriculture production, crop cultivating technology field, particularly relate under a kind of floating seedling cultivation of fire-cured tobacco condition, the matrix formulations of production Mycorrhizal cigarette seedling.
Background technology
Flue-cured tobacco is the important economic crops of China, occupies critical role in Chinese national economy.The tobacco planting area was 1,050,000 hectares in 2007, and tax revenue reaches 3,880 hundred million yuan, occupied first of the national every profession and trade individual event tax revenue.In cured tobacco production, cultivating strong sprout is one of important step of flue-cured tobacco cultivation, also is the important measures that improve yield of flue-cured tobacco and quality.At present, floating seedlings is the main mode of flue-cured tobacco seedling-cultivating, accounts for 85% of cigarette seedling output.Floating seedlings carries out in greenhouse or booth, is carrier with matrix, provides nutrient with nutrient solution.But in the practice of floating seedlings, the illumination in greenhouse or the booth is poor, and high humidity causes the excessive growth of cigarette seedling easily, the plant water content height, and supporting tissue is undeveloped.In addition, after the cigarette transplantation of seedlings, seedling-slowing stage is long partially, and drought-resistant ability is relatively poor, is unfavorable for the flue-cured tobacco high yield and high quality.
Cultivating strong sprout is the basis of flue-cured tobacco good quality and high output, attaches great importance to the flue-cured tobacco cultivation in strong sprout both at home and abroad.Inoculate good arbuscular mycorrhizal fungi (being called for short AM), infect formation Mycorrhizal cigarette seedling and can improve cigarette seedling nutrition condition, promote physiological metabolism, strengthen resistance, improve cigarette seedling quality, cultivate strong sprout, and then be of value to raising yield of tobacco and quality.In addition, the AM fungi is also playing an important role aspect maintenance soil and the plant health.For a long time, people are exploring the technical method of mycorhiza AM biological technology of mycorrhiza being pushed to practical application always.
In floating seedling cultivation of fire-cured tobacco, moisture is near saturated nutrient abundance, and the AM fungi growth is grown and is suppressed, and is difficult to infect host's root system and forms mycorhiza.Matrix is the environment of cigarette shoot root system growth, and the formation of mycorhiza and growth and matrix are closely related.In addition, moisture and nutrition obtain by matrix, and the composition of matrix directly influences the formation of cigarette seedling mycorhiza and the growth of mycorhiza seedling.Especially it should be noted that the AM fungi still can not pure culture, the microbial inoculum cost is higher.After matrix is selected, also need supporting with it inoculation technique, reduce the microbial inoculum consumption, improve postvaccinal infection rate.But, still do not have both at home and abroad at present under the floating seedlings condition, be fit to cultivate the dedicated substrate prescription and the corresponding inoculation technique of Mycorrhizal cigarette seedling, there is not relevant research report yet.So the dedicated substrate prescription and the corresponding inoculation technique of development Mycorrhizal cigarette seedling are pressing for of China's floating seedling cultivation of fire-cured tobacco intensification production.
Summary of the invention
The object of the present invention is to provide a kind ofly under the floating seedling cultivation of fire-cured tobacco condition, be fit to cultivate the dedicated substrate prescription of Mycorrhizal cigarette seedling, be used for production Mycorrhizal cigarette seedling, improve cigarette seedling overall qualities, cultivate strong sprout.
The component of the matrix formulations of floating seedling cultivation of fire-cured tobacco cultivation Mycorrhizal cigarette seedling of the present invention and the volumn concentration of each component are: peat 60%, perlite 20%, vermiculite 20%.
Base starting material requires to be preferably peat and crosses the 2mm sieve; Diameter 3～the 4mm of perlite particle; Diameter 3～the 4mm of vermiculite granule.
The inoculation method that floating seedling cultivation of fire-cured tobacco of the present invention is cultivated Mycorrhizal cigarette seedling formulation media is:
1, presses 1m
3Matrix is evenly sprayed 2% formalin 5L, sterilization back airing in airtight 7 days, and repeated multiple times is stirred in the airing process;
2, the matrix behind the sterilization is placed the hole of growing seedlings of floating dish, be filled to the height apart from top 10mm, the inoculum of thick 5mm is put in the shop; Broadcast 3 tobacco seeds, cover seed with thick 2mm river sand again, it is moistening to add water; Place the mycorrhizal seedling raising nutrient solution, cultivate the cigarette seedling according to the method for floating seedlings; The back 20 days even seedlings of emerging stay the best cigarette seedling of a strain growing way, continue to be cultured to be a seedling.
In the step 2 the mycorrhizal seedling raising nutrient solution prescription following (number of patent application: 200810069228.0):
Contain KH in every 1L nutrient solution
2PO
4Mother liquor 0.08ml, KNO
3Mother liquor 0.4ml, Ca (NO
3)
2Mother liquor 0.4ml, MgSO
4Mother liquor 0.16ml, micro-mother liquor 0.5ml, EDTA-mother liquid of iron salt 0.5ml, all the other are water;
KH
2PO
4, KNO
3, Ca (NO
3)
2, MgSO
4, and the concentration of EDTA-mother liquid of iron salt be 1mol/L;
The trace element mother liquor is a mixed solution, prepares in following ratio:
2.90g H
3BO
3, 1.86g MnCl
24H
2O, 0.28g ZnSO
47H
2O, 0.08g CuSO
45H
2O and 0.02gH
2MoO
4H
2O is dissolved in water respectively, adds 50ml concentrated sulfuric acid mixing, and the adding distil water dilution is settled to 1L, promptly is mixed with the trace element solution mother liquor;
The EDTA-mother liquid of iron salt, prepare in following ratio:
5.57g FeSO
47H
2O and 7.5g Na-EDTA reagent dissolve respectively to mix and are settled to 1L and are lmol/L EDTA-Fe.
Advantage of the present invention is:
1, it is wide to form the raw material sources of this matrix, easily purchases.
2, this matrix preparation is produced simple easily.
3, inoculation is simple to operate, and the microbial inoculum consumption is few, promotes easily.With sacculus mould (Glomus intraradices Smith﹠amp in this culture matrix, inoculation technique and the corresponding root; Schenck, BEG Number 141 is called for short BEG-141) triplicity, the mycorhiza infection rate is up to more than 40%, and the mycorhiza effect is good.
Embodiment
Embodiment 1:
Got the peat 0.6m of 2mm sieve respectively
3, diameter 3～4mm perlite 0.2m
3, diameter 3～4mm vermiculite 0.2m
3, fully mix, be mixed with the Mycorrhizal Friendly tobacco seedling raising medium; Formaldehyde 250ml with 40% converts water and is diluted to 5L, the formalin of preparation 2%; Get above-mentioned matrix 1m
3, 2% formaldehyde 5L is sprayed on the matrix uniformly, stir, place 1m
3In the cement pit, plastic covering film, and strict sealing, sterilization 7 days is spread into the thick thin layer of 3～4cm out after the taking-up, shine to dry in the air 7 days, and repeated multiple times is stirred in the airing process; Select the special-purpose seedling-cultivating tray of flue-cured tobacco in 90～120 holes, pack into matrix behind the sterilization, at the height of distance top 10mm, the shop put one deck 5mm thick, expand the mould inoculum of sacculus (Glomuse mosseae Gerdemann in the root after numerous with clover and corn; Trappe, BEG Number167 is called for short BEG-167), then, broadcast 3 tobacco seeds (kind is K326), cover seed with the 2mm river sand again, it is moistening to add water; Seedling-cultivating tray is placed nutrient solution, and (per 1 L nutrient solution contains 0.08ml 1mol/L KH
2PO
4Mother liquor, 0.4ml1mol/L KNO
3Mother liquor, 0.4ml 1mol/L Ca (NO
3)
2Mother liquor, 0.16ml 1mol/L MgSO
4Mother liquor, 0.5ml trace element mother liquor, 0.5ml1mol/L EDTA-mother liquid of iron salt, all the other are water), be cultured to be a seedling by the management of floating seedling cultivation of fire-cured tobacco and the fertilizing method of fungus rooting floating raise seedling.Microscopy becomes the cigarette seedling in seedling stage, AM infection rate 35%.
Embodiment 2:
Got the peat 3m of 2mm sieve respectively
3, diameter 3～4mm perlite 1m
3, diameter 3～4 mm vermiculite 1m
3, fully mix, be mixed with the Mycorrhizal Friendly tobacco seedling raising medium; Formaldehyde 750ml with 40% converts water and is diluted to 15L, the formalin of preparation 2%; Get above-mentioned matrix 3m
3, 2% formaldehyde 15L is sprayed on the matrix uniformly, stir, place 3m
3In the cement pit, plastic covering film, and strict sealing, sterilization 7 days is spread into the thick thin layer of 3～4cm out after the taking-up, shine to dry in the air 7 days, and repeated multiple times is stirred in the airing process; Select the special-purpose seedling-cultivating tray of flue-cured tobacco in 90～120 holes, pack into matrix behind the sterilization, at the height of distance top 10mm, the shop put one deck 5mm thick, expand the mould inoculum of sacculus (Glomuse mosseae Gerdemann in the root after numerous with clover and corn; Trappe, BEG Number167 is called for short BEG-167 and Glomus intraradices Smith ﹠amp; Schenck, BEG Number 141 is called for short BEG-141), then, broadcast 3 tobacco seeds (kind is K326), cover seed with the 2mm river sand again, it is moistening to add water; Seedling-cultivating tray is placed nutrient solution, and (every 1L nutrient solution contains 0.08ml 1mol/L KH
2PO
4Mother liquor, 0.4ml1mol/L KNO
3Mother liquor, 0.4ml1mol/L Ca (NO
3)
2Mother liquor, 0.16ml 1mol/L MgSO
4Mother liquor, 0.5ml trace element mother liquor, 0.5ml1mol/L EDTA-mother liquid of iron salt, all the other are water), be cultured to be a seedling by the management of floating seedling cultivation of fire-cured tobacco and the fertilizing method of fungus rooting floating raise seedling.Microscopy becomes the cigarette seedling in seedling stage, AM infection rate 40%.
Embodiment 3:
Got the peat 3m of 2mm sieve respectively
3, diameter 3～4mm perlite 1m
3, diameter 3～4mm vermiculite 1m
3, fully mix, be mixed with the Mycorrhizal Friendly tobacco seedling raising medium; Formaldehyde 750ml with 40% converts water and is diluted to 15L, the formalin of preparation 2%; Get above-mentioned matrix 3m
3, 2% formaldehyde 15L is sprayed on the matrix uniformly, stir, place 3m
3In the cement pit, plastic covering film, and strict sealing, sterilization 7 days is spread into the thick thin layer of 3～4cm out after the taking-up, shine to dry in the air 7 days, and repeated multiple times is stirred in the airing process; Select the special-purpose seedling-cultivating tray of flue-cured tobacco in 90～120 holes, pack into matrix behind the sterilization, at the height of distance top 10mm, the shop put one deck 5mm thick, expand the mould inoculum of sacculus (Glomuse mosseae Gerdemann in the root after numerous with clover and corn; Trappe, BEG Number167 is called for short BEG-167; Glomuse etunicatum Becker ﹠amp; Gerdemann, BEG Number168 is called for short BEG-168 and Glomus intraradices Smith ﹠amp; Schenck, BEG Number 141 is called for short BEG-141), then, broadcast 3 tobacco seeds (kind is K326), cover seed with the 2mm river sand again, it is moistening to add water; Seedling-cultivating tray is placed nutrient solution, and (every 1L nutrient solution contains 0.08ml 1mol/L KH
2PO
4Mother liquor, 0.4ml1mol/L KNO
3Mother liquor, 0.4ml 1mol/LCa (NO
3)
2Mother liquor, 0.16ml 1mol/L MgSO
4Mother liquor, 0.5ml trace element mother liquor, 0.5ml 1mol/L EDTA-mother liquid of iron salt, all the other are water), be cultured to be a seedling by the management of floating seedling cultivation of fire-cured tobacco and the fertilizing method of fungus rooting floating raise seedling.Microscopy becomes the cigarette seedling in seedling stage, AM infection rate 45%.
Claims (2)
1, the matrix formulations of floating seedlings production Mycorrhizal cigarette seedling is characterized in that, the component of this prescription and the volumn concentration of each component are: peat 60%, perlite 20%, vermiculite 20%.
2, prescription according to claim 1 is characterized in that, peat is crossed the 2mm sieve; Diameter 3～the 4mm of perlite particle; Diameter 3～the 4mm of vermiculite granule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100698272A CN101297632A (en) | 2008-06-13 | 2008-06-13 | Medium prescription for production of fungus-treated tobacco rooted seedlings by floating nursery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100698272A CN101297632A (en) | 2008-06-13 | 2008-06-13 | Medium prescription for production of fungus-treated tobacco rooted seedlings by floating nursery |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101297632A true CN101297632A (en) | 2008-11-05 |
Family
ID=40077691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100698272A Pending CN101297632A (en) | 2008-06-13 | 2008-06-13 | Medium prescription for production of fungus-treated tobacco rooted seedlings by floating nursery |
Country Status (1)
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138429A (en) * | 2011-04-11 | 2011-08-03 | 郭保银 | Cured tobacco seedling culturing method |
| CN102224787A (en) * | 2011-04-29 | 2011-10-26 | 云南省烟草农业科学研究院 | Method for producing mycorrhizal tobacco seedings by utilizing moist and float seedling culturing manner |
| CN102771362A (en) * | 2012-07-30 | 2012-11-14 | 河南农业大学 | Tobacco floating seedling culture medium and preparation method thereof |
| CN103229667A (en) * | 2013-04-27 | 2013-08-07 | 北京林业大学 | Mycorrhizal inoculum and preparing method and use thereof |
| CN104885889A (en) * | 2015-05-12 | 2015-09-09 | 河南艾农生物科技有限公司 | Matrix for tobacco floating seedling |
| CN106577004A (en) * | 2016-12-30 | 2017-04-26 | 广东中烟工业有限责任公司 | Composite, preparation method of composite and application of composite to tobacco leaf float seedling substrate field |
-
2008
- 2008-06-13 CN CNA2008100698272A patent/CN101297632A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138429A (en) * | 2011-04-11 | 2011-08-03 | 郭保银 | Cured tobacco seedling culturing method |
| CN102138429B (en) * | 2011-04-11 | 2012-01-25 | 郭保银 | Cured tobacco seedling culturing method |
| CN102224787A (en) * | 2011-04-29 | 2011-10-26 | 云南省烟草农业科学研究院 | Method for producing mycorrhizal tobacco seedings by utilizing moist and float seedling culturing manner |
| CN102771362A (en) * | 2012-07-30 | 2012-11-14 | 河南农业大学 | Tobacco floating seedling culture medium and preparation method thereof |
| CN103229667A (en) * | 2013-04-27 | 2013-08-07 | 北京林业大学 | Mycorrhizal inoculum and preparing method and use thereof |
| CN103229667B (en) * | 2013-04-27 | 2015-06-10 | 北京林业大学 | Mycorrhizal inoculum and preparing method and use thereof |
| CN104885889A (en) * | 2015-05-12 | 2015-09-09 | 河南艾农生物科技有限公司 | Matrix for tobacco floating seedling |
| CN106577004A (en) * | 2016-12-30 | 2017-04-26 | 广东中烟工业有限责任公司 | Composite, preparation method of composite and application of composite to tobacco leaf float seedling substrate field |
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Open date: 20081105 |