CN101273734A - Smokes applied in producing edible or medicinal fungi for sterilization - Google Patents
Smokes applied in producing edible or medicinal fungi for sterilization Download PDFInfo
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- CN101273734A CN101273734A CNA2008100639715A CN200810063971A CN101273734A CN 101273734 A CN101273734 A CN 101273734A CN A2008100639715 A CNA2008100639715 A CN A2008100639715A CN 200810063971 A CN200810063971 A CN 200810063971A CN 101273734 A CN101273734 A CN 101273734A
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- neurospora
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Abstract
The invention discloses a sterilization smoke agent applied to the production of edible and medicinal fungus, the dust amount is only 0.2823g/m<2> after lighting the agent. The smoke agent spreads fast, the scientific combination of the germicide has obvious synergism, showing rather strong toxicity in a combined toxicity experiment. The smoke agent of the invention is a mixture and formed by crashing and mixing solid materials, the formulation is 8 to 12 percent of daconil ( 96 percent of effective content ), 6 to 8 percent of urotropine, 2 to 4 percent of nitre, 8 to 12 percent of paraformaldehyde and the rest of sodium dichloro cyanurate.
Description
Technical field
A kind of be used to put out, prevent edible and medical fungi cultivate between the bactericide that spreads of assorted bacterium such as Neurospora, specifically a kind of fumicants.
Technical background
The desirable air purity of edible and medical fungi culturing room is ten thousand grades of 10-30.Common facility can't reach this standard.Various microorganisms such as kind of fungal spore, yeast, actinomyces surplus kind of the bacterium, 600 are suspending in the air surplus in the of 160.Competitive assorted bacterium often causes the failure of mushroom class cultivation.Bread beading mould (being commonly called as Neurospora, asexual generation Monilia sitophila Mont., sexual generation Neurosoura sitophila), spore diameter 10-12 micron.25 ℃ of-30 ℃ of conditions were sprouted in 6 hours, formed mycelia, 48 hours formation spores, rapid spread.
Chinese patent, application number are 02100362.9, and name is called " a kind of microbicide compositions " and also discloses a kind of sterilization fumicants, and it comprises at least a acryloyl morpholine series bactericidal agent and Bravo.The match ratio of acryloyl morpholine series bactericidal agent and Bravo is 1: 70-20: 1.Above-mentioned composition is mixed with sterilization fumicants, controlling plant diseases.But this fumicants has an innings property to the treatment effect in the big generation of Neurospora place.At present, the popular Neurospora bactericide of explosion type is not still had active drug, prevent, put out the big generation of Neurospora and lack technical measures.
Summary of the invention
The object of the invention is to disclose and a kind ofly is applied to eat/sterilization fumicants that medicinal bacterium produces, and it only is 0.2823g/m that preparation is lighted the back amount of dust
2The fumicants diffusion is fast, and the science combination of bactericide has obvious synergistic effect, demonstrates to have very strong virulence in the co-toxicity test.
Fumicants of the present invention is a mixture, mixes after solids is pulverized, and filling a prescription is:
Bravo (effective content 96%) 8-12%, methenamine 6-8%, potassium nitrate 2-4%, paraformaldehyde 8-12%, all the other are sodium dichloro cyanurate.
Bravo (2,4,5,6-tetrachloro-1, the 3-benzene dicarbonitrile, chlorothalonil) fungicidal spectrum is wide.6 days half-life, fusing point 250-251 ℃, can make the host of fumicants.Sodium dichloro cyanurate (Babysafe, CDB
63, Sodium dichloroisocryanuranurate, C
3O
3N
3Cl
2Na M=219.9) is industrial bactericide, approved product is arranged.The two is used with, through measuring with filter paper method and Pfennig method, proves that co-toxicity has summation action.Be used as fumicants, certain effect is arranged.
To being carried out integrated conduct method be sealing source-dispensing fumicants-sprinkling aqua-scrub facility-aeration-drying-dispensing fumicants by the culturing room of Neurospora severe contamination.Polluting material will deal with, and contaminated raw material are admixed bactericide, and autoclaving is reused after residual medicament is degraded to safe concentration.
The specific embodiment
Embodiment 1
Mixture of the present invention, filling a prescription is:
Bravo (effective content 96%) 8%, methenamine 6%, potassium nitrate 2%, paraformaldehyde 8%, sodium dichloro cyanurate (effective content 60%) 76%; To mix after the solids pulverizing.
Embodiment 2
Mixture of the present invention, filling a prescription is:
Bravo (effective content 96%) 10%, methenamine 7%, potassium nitrate 3%, paraformaldehyde 10%, sodium dichloro cyanurate (effective content 60%) 70%; To mix after the solids pulverizing.
Embodiment 3
Mixture of the present invention, filling a prescription is:
Bravo (effective content 96%) 12%, methenamine 8%, potassium nitrate 4%, paraformaldehyde 12%, sodium dichloro cyanurate (effective content 60%) 66%; To mix after the solids pulverizing.
The present invention is to the processing in the big generation of Neurospora place:
The big place that takes place of sealing, preparation fumicants, dosage 8-12g/m
3, tightly sealed 24 hours.The preparation aqua sprays uses aqua, Bravo (96%) 4%, and sodium dichloro cyanurate (60%) 5% sprays contaminated bacteria bag, ground, wall, support, door and window, ceiling, ventilating opening, corridor comprehensively.Shift out the bacterium bag that surperficial with medicament was sprayed, make fertilizer or buried behind the autoclaving.Scrub the wiped part of cement or brick floor file face, heating equipment, air vent, cable, lighting apparatus.Aqua with thiophanate methyl (7%) sodium dichloro cyanurate (5%) is scrubbed comprehensively.All metal objects are scrubbed the back with the clear water flushing, wipe clean.Each dead angle is all fully scrubbed.After scrubbing, open door and window and ventilate, to reduce the air humidity of handling the place.Spray with Bravo (96%) 4%, the aqua of sodium dichloro cyanurate (60%) 5% on body of wall, accumulation article, ground outside the culturing room.Culturing room presses fumicants and handles dosage 5g/m after above-mentioned steps is handled
3
As be basement, with Bravo (effective content 96%) 76%, sulphur powder 4%, the fumicants of methenamine 20% is handled, dosage 6-8g/m
3
Effect
First experiment
Neurospora drops out existing probability after using fumicants of the present invention, dispenser after 6 hours chain spore enzyme probability to occur be 0.
Second batch of experiment
Preparation is to the virulence of Neurospora, and clump count (Cfu/ ware) background is 0.32 ± 0.62, dispenser with 6hr after clump count be 0.
The production application example
Place 1: Liu Xiu cloud mushroom room, Harbin, time 2002-03-12
The culturing room of Shi Yonging for many years, the big generation of Neurospora during test.The V=290m of culturing room
3, fumicants numbering 1.1a.30 in background sampling culture dish all detects Neurospora, and is covered with culture dish, other assorted bacterium 82.4cfu/ wares on each ware.
Dosage 7g/m
3, airtight 24 hours.Sampling (30 culture dishes), only No. 9 culture dish detects a bacterial clump.30 culture dishes all do not detect Neurospora.
Virulence: to Neurospora 100%, comprehensive virulence 99.97%.
Place 2: lotus flower township, Linkou County, Mudanjiang City edible mushroom factory, time 2003-04-07 to 2003-04-09.
Neurospora takes place in culturing room, and Neurospora appears in sampling culture dish 37.5%.Culturing room is 500m altogether
3, preparation 1.1a, medication 2 times, each 10g/m
3, medication is taken a sample after 6 hours, is cultivated.Do not detect Neurospora and any assorted bacterium.Adopt the listed aggregate measures reason of the application simultaneously, return to normal production.
Place 3: forest farm, Fangzheng County, Heilongjiang Province, in mid-December, 2000 time.
Entirely for Neurospora pollutes, use the application's preparation between 2 cultivations.Dosage 8g/m
3, sampling after 12 hours.Do not detect Neurospora and other assorted bacterium in 20 φ 90mm culture dishes.Virulence 100%.
Place 4: the emerald green mountains in a range self-employed worker in Yichun, Heilongjiang Province, time: 2002-01-22.
The application uses 2 times, each 5g/m
3, V=1000m between cultivation
3, the Neurospora that has spread is extinguished.
Claims (1)
1. one kind is applied to eat/sterilization fumicants that medicinal bacterium produces, is mixture, will mix after the solids pulverizing, and filling a prescription is:
Bravo (effective content 96%) 8-12%, methenamine 6-8%, potassium nitrate 2-4%, paraformaldehyde 8-12%, all the other are sodium dichloro cyanurate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100639715A CN101273734B (en) | 2008-02-01 | 2008-02-01 | Smokes applied in producing edible or medicinal fungi for sterilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100639715A CN101273734B (en) | 2008-02-01 | 2008-02-01 | Smokes applied in producing edible or medicinal fungi for sterilization |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101273734A true CN101273734A (en) | 2008-10-01 |
| CN101273734B CN101273734B (en) | 2012-06-13 |
Family
ID=39993932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100639715A Expired - Fee Related CN101273734B (en) | 2008-02-01 | 2008-02-01 | Smokes applied in producing edible or medicinal fungi for sterilization |
Country Status (1)
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| CN (1) | CN101273734B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101543241B (en) * | 2009-05-04 | 2011-05-11 | 中国科学院华南植物园 | Combustible fruit and vegetable smoke formulation fresh-keeping agent |
| CN102369960A (en) * | 2010-08-20 | 2012-03-14 | 河南亚卫动物药业有限公司 | Solid fumigation disinfectant and preparation method thereof |
| CN103858934A (en) * | 2014-03-18 | 2014-06-18 | 中国农业科学院兰州畜牧与兽药研究所 | Veterinary suffumigation medicine composition and preparation method and application thereof |
| CN104224874A (en) * | 2013-06-08 | 2014-12-24 | 虎尾科技大学 | Asarum extract and application thereof |
| CN105875819A (en) * | 2016-05-11 | 2016-08-24 | 龙岩泰华实业有限公司 | Antibacterial fruit preservative as well as preparation and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1371606A (en) * | 2002-01-15 | 2002-10-02 | 王现全 | Sterilizing composition |
| CN1575657A (en) * | 2003-07-29 | 2005-02-09 | 勾刚 | Medicament for preventing and curing Neurospora |
| GB0422401D0 (en) * | 2004-10-08 | 2004-11-10 | Syngenta Participations Ag | Fungicidal compositions |
-
2008
- 2008-02-01 CN CN2008100639715A patent/CN101273734B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101543241B (en) * | 2009-05-04 | 2011-05-11 | 中国科学院华南植物园 | Combustible fruit and vegetable smoke formulation fresh-keeping agent |
| CN102369960A (en) * | 2010-08-20 | 2012-03-14 | 河南亚卫动物药业有限公司 | Solid fumigation disinfectant and preparation method thereof |
| CN102369960B (en) * | 2010-08-20 | 2013-10-02 | 河南亚卫动物药业有限公司 | Solid fumigation disinfectant and preparation method thereof |
| CN104224874A (en) * | 2013-06-08 | 2014-12-24 | 虎尾科技大学 | Asarum extract and application thereof |
| CN103858934A (en) * | 2014-03-18 | 2014-06-18 | 中国农业科学院兰州畜牧与兽药研究所 | Veterinary suffumigation medicine composition and preparation method and application thereof |
| CN103858934B (en) * | 2014-03-18 | 2016-03-02 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of fumigation pharmaceutical composition for animals and its preparation method and application |
| CN105875819A (en) * | 2016-05-11 | 2016-08-24 | 龙岩泰华实业有限公司 | Antibacterial fruit preservative as well as preparation and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101273734B (en) | 2012-06-13 |
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Owner name: HEILONGJIANG RUIFENGYING MODERN AGRICULTURE TECHNO Free format text: FORMER OWNER: KANG KEXIN Effective date: 20130221 |
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Effective date of registration: 20130221 Address after: 150010 No. 1, 3 floor, 41 Wenchang street, Nangang District, Heilongjiang, Harbin Patentee after: Heilongjiang Ruifeng modern agricultural science and Technology Co., Ltd. Address before: 150008 No. 8 Yanhe Road, Nangang District, Heilongjiang, Harbin Patentee before: Kang Kexin |
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