CN101268192A - 包含由植物病毒外壳蛋白、肽接头和重组蛋白组成的多种融合蛋白的植物病毒颗粒及此类植物病毒颗粒用于蛋白质纯化的用途 - Google Patents

包含由植物病毒外壳蛋白、肽接头和重组蛋白组成的多种融合蛋白的植物病毒颗粒及此类植物病毒颗粒用于蛋白质纯化的用途 Download PDF

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CN101268192A
CN101268192A CNA2006800340346A CN200680034034A CN101268192A CN 101268192 A CN101268192 A CN 101268192A CN A2006800340346 A CNA2006800340346 A CN A2006800340346A CN 200680034034 A CN200680034034 A CN 200680034034A CN 101268192 A CN101268192 A CN 101268192A
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protein
virus
recombinant
particle
plant
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Chinese (zh)
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S·维尔纳
S·马里约内
V·克利姆克
Y·格莱巴
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Icon Genetics AG
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Icon Genetics AG
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
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    • C12N2770/00011—Details
    • C12N2770/00041—Use of virus, viral particle or viral elements as a vector
    • C12N2770/00043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/26011—Flexiviridae
    • C12N2770/26023—Virus like particles [VLP]

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  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CNA2006800340346A 2005-09-17 2006-09-15 包含由植物病毒外壳蛋白、肽接头和重组蛋白组成的多种融合蛋白的植物病毒颗粒及此类植物病毒颗粒用于蛋白质纯化的用途 Pending CN101268192A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05020311.6 2005-09-17
EP05020311A EP1764414A1 (en) 2005-09-17 2005-09-17 Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification

Publications (1)

Publication Number Publication Date
CN101268192A true CN101268192A (zh) 2008-09-17

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CNA2006800340346A Pending CN101268192A (zh) 2005-09-17 2006-09-15 包含由植物病毒外壳蛋白、肽接头和重组蛋白组成的多种融合蛋白的植物病毒颗粒及此类植物病毒颗粒用于蛋白质纯化的用途

Country Status (8)

Country Link
US (1) US20090062514A1 (enExample)
EP (2) EP1764414A1 (enExample)
JP (1) JP2009507503A (enExample)
KR (1) KR20080065613A (enExample)
CN (1) CN101268192A (enExample)
AU (1) AU2006291435A1 (enExample)
CA (1) CA2621243A1 (enExample)
WO (1) WO2007031339A1 (enExample)

Cited By (8)

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Publication number Priority date Publication date Assignee Title
WO2017020570A1 (en) * 2015-08-06 2017-02-09 Medigen Biotechnology Corp. Virus-like particle vaccines
CN106566842A (zh) * 2016-07-18 2017-04-19 湖南农业大学 一种蛋白或多肽的植物表达载体及其应用
CN107771218A (zh) * 2015-06-17 2018-03-06 先锋国际良种公司 植物调节元件及其使用方法
CN112566494A (zh) * 2018-06-12 2021-03-26 肯塔基生物处理股份有限公司 病毒和抗原纯化和偶联
US11529413B2 (en) 2018-06-12 2022-12-20 Kbio Holdings Limited Virus and antigen purification and conjugation
US11690907B2 (en) 2018-06-12 2023-07-04 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
US11696948B2 (en) 2018-06-12 2023-07-11 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
WO2024193624A1 (zh) * 2023-03-21 2024-09-26 深圳赫兹生命科学技术有限公司 去势tmv颗粒亚单位疫苗及其制备方法

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EP2215223B1 (en) * 2007-10-31 2013-05-01 Precision Biosciences, Inc. Rationally-designed single-chain meganucleases with non-palindromic recognition sequences
EP2500035A1 (en) 2011-03-15 2012-09-19 Icon Genetics GmbH Pharmaceutical formulation containing immunglobulin
US10117923B2 (en) 2012-11-07 2018-11-06 Vacplanta Limited Production of an immunogen using a plant virus
WO2014074509A1 (en) * 2012-11-07 2014-05-15 Vacplanta Limted Production of an immunogen using a plant virus
EP2964032A4 (en) * 2013-03-05 2016-11-23 Shifa Biomedical COMPOSITIONS AND METHOD FOR THE PRODUCTION OF VIRUSELIC PARTICLES
EP2777711A1 (en) 2013-03-11 2014-09-17 Icon Genetics GmbH Her2/Neu cancer vaccine
EP3085774A1 (en) * 2015-04-20 2016-10-26 QIAGEN GmbH Method for concentrating, purifying and/or isolating virus particles
WO2017051434A1 (en) * 2015-09-22 2017-03-30 Indian Institute Of Science Chimeric plant based virus-like-particles, and uses thereof
CN108956983B (zh) * 2018-05-07 2021-05-07 西北工业大学 一种以烟草花叶病毒为模板的微传感器可控阵列化制备方法

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US6660500B2 (en) * 1988-02-26 2003-12-09 Large Scale Biology Corporation Production of peptides in plants as viral coat protein fusions
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5977438A (en) 1988-02-26 1999-11-02 Biosource Technologies, Inc. Production of peptides in plants as viral coat protein fusions
IT1249603B (it) 1991-02-21 1995-03-09 Cremona Angelo & Figlio Stazione di taglio automatizzata per tranciati di legno
JP2880024B2 (ja) * 1992-03-31 1999-04-05 鐘紡株式会社 植物ウィルスベクター及びプラスミド並びに外来遺伝子を植物中で発現させる方法
EP1162267A3 (en) * 1992-03-31 2002-12-18 Kanebo Limited Plant virus vector, plasmid, process for expression of foreign gene and process for obtaining foreign gene product
JPH06169789A (ja) * 1992-12-08 1994-06-21 Kanebo Ltd 外来遺伝子産物の取得方法
US5491076A (en) 1993-11-01 1996-02-13 The Texas A&M University System Expression of foreign genes using a replicating polyprotein producing virus vector
US5670353A (en) 1994-08-25 1997-09-23 Mycogen Plant Science, Inc. Subgenomic promoter
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JP3936661B2 (ja) * 2001-01-18 2007-06-27 アイコン・ジェネティクス，インコーポレイテッド タンパク質を検出または精製するためのウィルスの使用
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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107771218A (zh) * 2015-06-17 2018-03-06 先锋国际良种公司 植物调节元件及其使用方法
WO2017020570A1 (en) * 2015-08-06 2017-02-09 Medigen Biotechnology Corp. Virus-like particle vaccines
CN106566842A (zh) * 2016-07-18 2017-04-19 湖南农业大学 一种蛋白或多肽的植物表达载体及其应用
CN106566842B (zh) * 2016-07-18 2019-04-19 湖南农业大学 一种蛋白或多肽的植物表达载体及其应用
US11529413B2 (en) 2018-06-12 2022-12-20 Kbio Holdings Limited Virus and antigen purification and conjugation
US11485956B2 (en) 2018-06-12 2022-11-01 Kbio Holdings Limited Virus and antigen purification and conjugation
CN112566494A (zh) * 2018-06-12 2021-03-26 肯塔基生物处理股份有限公司 病毒和抗原纯化和偶联
US11655461B2 (en) 2018-06-12 2023-05-23 Kbio Holdings Limited Antigen purification
US11690907B2 (en) 2018-06-12 2023-07-04 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
US11696948B2 (en) 2018-06-12 2023-07-11 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
US12097256B2 (en) 2018-06-12 2024-09-24 Kbio Holdings Limited Virus and antigen purification and conjugation
US12173328B2 (en) 2018-06-12 2024-12-24 Kbio Holdings Limited Virus and antigen conjugation
WO2024193624A1 (zh) * 2023-03-21 2024-09-26 深圳赫兹生命科学技术有限公司 去势tmv颗粒亚单位疫苗及其制备方法

Also Published As

Publication number Publication date
WO2007031339A1 (en) 2007-03-22
EP1926819A1 (en) 2008-06-04
EP1764414A1 (en) 2007-03-21
EP1926819B1 (en) 2013-01-16
CA2621243A1 (en) 2007-03-22
AU2006291435A1 (en) 2007-03-22
KR20080065613A (ko) 2008-07-14
AU2006291435A8 (en) 2008-05-15
US20090062514A1 (en) 2009-03-05
JP2009507503A (ja) 2009-02-26

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Application publication date: 20080917