KR20080065613A - 식물 바이러스 외피 단백질, 펩티드 링커 및 재조합단백질로 구성된 다수의 융합 단백질을 포함하는 식물바이러스 입자 및 상기 식물 바이러스 입자의 단백질정제용 용도 - Google Patents

식물 바이러스 외피 단백질, 펩티드 링커 및 재조합단백질로 구성된 다수의 융합 단백질을 포함하는 식물바이러스 입자 및 상기 식물 바이러스 입자의 단백질정제용 용도 Download PDF

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KR20080065613A
KR20080065613A KR1020087008611A KR20087008611A KR20080065613A KR 20080065613 A KR20080065613 A KR 20080065613A KR 1020087008611 A KR1020087008611 A KR 1020087008611A KR 20087008611 A KR20087008611 A KR 20087008611A KR 20080065613 A KR20080065613 A KR 20080065613A
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protein
virus
recombinant
plant
particle
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Korean (ko)
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스테판 워너
실베스트레 마릴로네트
빅터 크림유크
유리 그레바
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아이콘 제네틱스 지엠비에이치
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00041—Use of virus, viral particle or viral elements as a vector
    • C12N2770/00043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/26011—Flexiviridae
    • C12N2770/26023—Virus like particles [VLP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
KR1020087008611A 2005-09-17 2006-09-15 식물 바이러스 외피 단백질, 펩티드 링커 및 재조합단백질로 구성된 다수의 융합 단백질을 포함하는 식물바이러스 입자 및 상기 식물 바이러스 입자의 단백질정제용 용도 Ceased KR20080065613A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05020311.6 2005-09-17
EP05020311A EP1764414A1 (en) 2005-09-17 2005-09-17 Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification

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KR20080065613A true KR20080065613A (ko) 2008-07-14

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KR1020087008611A Ceased KR20080065613A (ko) 2005-09-17 2006-09-15 식물 바이러스 외피 단백질, 펩티드 링커 및 재조합단백질로 구성된 다수의 융합 단백질을 포함하는 식물바이러스 입자 및 상기 식물 바이러스 입자의 단백질정제용 용도

Country Status (8)

Country Link
US (1) US20090062514A1 (enExample)
EP (2) EP1764414A1 (enExample)
JP (1) JP2009507503A (enExample)
KR (1) KR20080065613A (enExample)
CN (1) CN101268192A (enExample)
AU (1) AU2006291435A1 (enExample)
CA (1) CA2621243A1 (enExample)
WO (1) WO2007031339A1 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2215223B1 (en) * 2007-10-31 2013-05-01 Precision Biosciences, Inc. Rationally-designed single-chain meganucleases with non-palindromic recognition sequences
EP2500035A1 (en) 2011-03-15 2012-09-19 Icon Genetics GmbH Pharmaceutical formulation containing immunglobulin
US10117923B2 (en) 2012-11-07 2018-11-06 Vacplanta Limited Production of an immunogen using a plant virus
WO2014074509A1 (en) * 2012-11-07 2014-05-15 Vacplanta Limted Production of an immunogen using a plant virus
EP2964032A4 (en) * 2013-03-05 2016-11-23 Shifa Biomedical COMPOSITIONS AND METHOD FOR THE PRODUCTION OF VIRUSELIC PARTICLES
EP2777711A1 (en) 2013-03-11 2014-09-17 Icon Genetics GmbH Her2/Neu cancer vaccine
EP3085774A1 (en) * 2015-04-20 2016-10-26 QIAGEN GmbH Method for concentrating, purifying and/or isolating virus particles
CA2985490A1 (en) * 2015-06-17 2016-12-22 E.I. Du Pont De Nemours And Company Plant regulatory elements and methods of use thereof
WO2017020570A1 (en) * 2015-08-06 2017-02-09 Medigen Biotechnology Corp. Virus-like particle vaccines
WO2017051434A1 (en) * 2015-09-22 2017-03-30 Indian Institute Of Science Chimeric plant based virus-like-particles, and uses thereof
CN106566842B (zh) * 2016-07-18 2019-04-19 湖南农业大学 一种蛋白或多肽的植物表达载体及其应用
CN108956983B (zh) * 2018-05-07 2021-05-07 西北工业大学 一种以烟草花叶病毒为模板的微传感器可控阵列化制备方法
KR102714626B1 (ko) * 2018-06-12 2024-10-08 케이바이오 홀딩스 리미티드 바이러스 및 항원 정제 및 접합
US11690907B2 (en) 2018-06-12 2023-07-04 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
US11529413B2 (en) 2018-06-12 2022-12-20 Kbio Holdings Limited Virus and antigen purification and conjugation
US11696948B2 (en) 2018-06-12 2023-07-11 Kbio Holdings Limited Vaccines formed by virus and antigen conjugation
CN118684781A (zh) * 2023-03-21 2024-09-24 深圳赫兹生命科学技术有限公司 GnRH-VLP重组去势疫苗及其制备方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466788A (en) 1985-03-07 1995-11-14 Mycogen Plant Science, Inc. Subgenomic promoter
US6660500B2 (en) * 1988-02-26 2003-12-09 Large Scale Biology Corporation Production of peptides in plants as viral coat protein fusions
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5977438A (en) 1988-02-26 1999-11-02 Biosource Technologies, Inc. Production of peptides in plants as viral coat protein fusions
IT1249603B (it) 1991-02-21 1995-03-09 Cremona Angelo & Figlio Stazione di taglio automatizzata per tranciati di legno
JP2880024B2 (ja) * 1992-03-31 1999-04-05 鐘紡株式会社 植物ウィルスベクター及びプラスミド並びに外来遺伝子を植物中で発現させる方法
EP1162267A3 (en) * 1992-03-31 2002-12-18 Kanebo Limited Plant virus vector, plasmid, process for expression of foreign gene and process for obtaining foreign gene product
JPH06169789A (ja) * 1992-12-08 1994-06-21 Kanebo Ltd 外来遺伝子産物の取得方法
US5491076A (en) 1993-11-01 1996-02-13 The Texas A&M University System Expression of foreign genes using a replicating polyprotein producing virus vector
US5670353A (en) 1994-08-25 1997-09-23 Mycogen Plant Science, Inc. Subgenomic promoter
DE10049587A1 (de) 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
JP3936661B2 (ja) * 2001-01-18 2007-06-27 アイコン・ジェネティクス，インコーポレイテッド タンパク質を検出または精製するためのウィルスの使用
EP2184363B1 (en) 2003-11-10 2013-08-28 Icon Genetics GmbH RNA virus-derived plant expression system
EP1686176A1 (en) 2005-01-28 2006-08-02 Icon Genetics AG Production of antibodies in plants with plus-sense single-stranded viral RNA vectors

Also Published As

Publication number Publication date
WO2007031339A1 (en) 2007-03-22
EP1926819A1 (en) 2008-06-04
EP1764414A1 (en) 2007-03-21
EP1926819B1 (en) 2013-01-16
CA2621243A1 (en) 2007-03-22
AU2006291435A1 (en) 2007-03-22
AU2006291435A8 (en) 2008-05-15
CN101268192A (zh) 2008-09-17
US20090062514A1 (en) 2009-03-05
JP2009507503A (ja) 2009-02-26

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