CA2621243A1 - Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification - Google Patents
Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification Download PDFInfo
- Publication number
- CA2621243A1 CA2621243A1 CA002621243A CA2621243A CA2621243A1 CA 2621243 A1 CA2621243 A1 CA 2621243A1 CA 002621243 A CA002621243 A CA 002621243A CA 2621243 A CA2621243 A CA 2621243A CA 2621243 A1 CA2621243 A1 CA 2621243A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- recombinant
- viral
- particles
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000002245 particle Substances 0.000 title claims abstract description 264
- 230000003612 virological effect Effects 0.000 title claims abstract description 213
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims abstract description 142
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims abstract description 142
- 102000037865 fusion proteins Human genes 0.000 title claims abstract description 130
- 108020001507 fusion proteins Proteins 0.000 title claims abstract description 130
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 92
- 108090000565 Capsid Proteins Proteins 0.000 title claims abstract description 91
- 238000001742 protein purification Methods 0.000 title description 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 198
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 187
- 238000000034 method Methods 0.000 claims abstract description 51
- 230000008569 process Effects 0.000 claims abstract description 25
- 241000196324 Embryophyta Species 0.000 claims description 175
- 239000011159 matrix material Substances 0.000 claims description 80
- 125000000539 amino acid group Chemical group 0.000 claims description 43
- 101710132601 Capsid protein Proteins 0.000 claims description 42
- 101710094648 Coat protein Proteins 0.000 claims description 42
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims description 42
- 101710125418 Major capsid protein Proteins 0.000 claims description 42
- 101710141454 Nucleoprotein Proteins 0.000 claims description 42
- 101710083689 Probable capsid protein Proteins 0.000 claims description 42
- 108060003951 Immunoglobulin Proteins 0.000 claims description 35
- 102000018358 immunoglobulin Human genes 0.000 claims description 35
- 210000004027 cell Anatomy 0.000 claims description 31
- 230000004927 fusion Effects 0.000 claims description 31
- 229940072221 immunoglobulins Drugs 0.000 claims description 29
- 239000013603 viral vector Substances 0.000 claims description 24
- 241000723873 Tobacco mosaic virus Species 0.000 claims description 22
- 241001147459 Turnip vein-clearing virus Species 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 13
- 102000040430 polynucleotide Human genes 0.000 claims description 12
- 108091033319 polynucleotide Proteins 0.000 claims description 12
- 239000002157 polynucleotide Substances 0.000 claims description 12
- 238000001261 affinity purification Methods 0.000 claims description 10
- 108700011201 Streptococcus IgG Fc-binding Proteins 0.000 claims description 7
- 210000004899 c-terminal region Anatomy 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 108010090804 Streptavidin Proteins 0.000 claims description 6
- 101710120037 Toxin CcdB Proteins 0.000 claims description 6
- 238000012384 transportation and delivery Methods 0.000 claims description 6
- 108010088160 Staphylococcal Protein A Proteins 0.000 claims description 5
- 208000015181 infectious disease Diseases 0.000 claims description 5
- 230000001404 mediated effect Effects 0.000 claims description 5
- 241000589158 Agrobacterium Species 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 230000002950 deficient Effects 0.000 claims description 2
- 230000002779 inactivation Effects 0.000 claims 1
- 238000009877 rendering Methods 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 235000018102 proteins Nutrition 0.000 description 163
- 241000894007 species Species 0.000 description 54
- 241000700605 Viruses Species 0.000 description 45
- 230000014509 gene expression Effects 0.000 description 31
- 238000000746 purification Methods 0.000 description 28
- 239000013256 coordination polymer Substances 0.000 description 24
- 241000709992 Potato virus X Species 0.000 description 21
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 17
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 17
- 239000005090 green fluorescent protein Substances 0.000 description 17
- 239000013598 vector Substances 0.000 description 14
- 230000009885 systemic effect Effects 0.000 description 12
- 239000012634 fragment Substances 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 108700019146 Transgenes Proteins 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 9
- 102000004196 processed proteins & peptides Human genes 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 150000003384 small molecules Chemical class 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000008188 pellet Substances 0.000 description 7
- 238000012552 review Methods 0.000 description 7
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 230000006798 recombination Effects 0.000 description 6
- 238000005215 recombination Methods 0.000 description 6
- 241000207746 Nicotiana benthamiana Species 0.000 description 5
- 150000001413 amino acids Chemical group 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 238000001493 electron microscopy Methods 0.000 description 5
- 238000001962 electrophoresis Methods 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 210000002845 virion Anatomy 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 108060004795 Methyltransferase Proteins 0.000 description 4
- 244000061176 Nicotiana tabacum Species 0.000 description 4
- 108050006628 Viral movement proteins Proteins 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000004132 cross linking Methods 0.000 description 4
- 239000003431 cross linking reagent Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 241001493065 dsRNA viruses Species 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 230000008595 infiltration Effects 0.000 description 4
- 238000001764 infiltration Methods 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 108010058731 nopaline synthase Proteins 0.000 description 4
- 230000012743 protein tagging Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 241000114864 ssRNA viruses Species 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- 241000724328 Alfalfa mosaic virus Species 0.000 description 3
- 241000723655 Cowpea mosaic virus Species 0.000 description 3
- 241000450599 DNA viruses Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241000702463 Geminiviridae Species 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 3
- 102100034343 Integrase Human genes 0.000 description 3
- 108010061833 Integrases Proteins 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 108700026244 Open Reading Frames Proteins 0.000 description 3
- 241000723762 Potato virus Y Species 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 241000723848 Tobamovirus Species 0.000 description 3
- 108020000999 Viral RNA Proteins 0.000 description 3
- 238000001042 affinity chromatography Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 108010011170 Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000724256 Brome mosaic virus Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241001310482 Cucumber fruit mottle mosaic virus Species 0.000 description 2
- 241000723845 Cucumber green mottle mosaic virus Species 0.000 description 2
- 101150062179 II gene Proteins 0.000 description 2
- 241000702459 Mastrevirus Species 0.000 description 2
- 102100035971 Molybdopterin molybdenumtransferase Human genes 0.000 description 2
- 101710119577 Molybdopterin molybdenumtransferase Proteins 0.000 description 2
- 229930193140 Neomycin Natural products 0.000 description 2
- 241000723826 Odontoglossum ringspot virus Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 241000500290 Ribgrass mosaic virus Species 0.000 description 2
- 241000961587 Secoviridae Species 0.000 description 2
- 101000582398 Staphylococcus aureus Replication initiation protein Proteins 0.000 description 2
- 108091027544 Subgenomic mRNA Proteins 0.000 description 2
- 241000723881 Sunn-hemp mosaic virus Species 0.000 description 2
- 241000723613 Tomato mosaic virus Species 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 108010067390 Viral Proteins Proteins 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 244000037640 animal pathogen Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 229920001222 biopolymer Polymers 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 229960004927 neomycin Drugs 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 230000005030 transcription termination Effects 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- OTIXUSNHAKOJBX-UHFFFAOYSA-N 1-(aziridin-1-yl)ethanone Chemical compound CC(=O)N1CC1 OTIXUSNHAKOJBX-UHFFFAOYSA-N 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 241000724330 Alfamovirus Species 0.000 description 1
- 235000016790 Allium chinense Nutrition 0.000 description 1
- 244000295724 Allium chinense Species 0.000 description 1
- 241000405760 Alphapartitivirus Species 0.000 description 1
- 241000710009 Apple chlorotic leaf spot virus Species 0.000 description 1
- 241001135987 Apple stem grooving virus Species 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 108700006678 Arabidopsis ACT2 Proteins 0.000 description 1
- 208000002109 Argyria Diseases 0.000 description 1
- NOWKCMXCCJGMRR-UHFFFAOYSA-N Aziridine Chemical compound C1CN1 NOWKCMXCCJGMRR-UHFFFAOYSA-N 0.000 description 1
- 241000701513 Badnavirus Species 0.000 description 1
- 241000724306 Barley stripe mosaic virus Species 0.000 description 1
- 241000709756 Barley yellow dwarf virus Species 0.000 description 1
- 241000724681 Barley yellow mosaic virus Species 0.000 description 1
- 241000702286 Bean golden mosaic virus Species 0.000 description 1
- 241000702325 Beet curly top virus Species 0.000 description 1
- 241000710149 Beet yellows virus Species 0.000 description 1
- 241000702451 Begomovirus Species 0.000 description 1
- 241000405758 Betapartitivirus Species 0.000 description 1
- 235000007689 Borago officinalis Nutrition 0.000 description 1
- 240000004355 Borago officinalis Species 0.000 description 1
- 241000372028 Broad bean wilt virus Species 0.000 description 1
- 241001533462 Bromoviridae Species 0.000 description 1
- 241000724268 Bromovirus Species 0.000 description 1
- 241001533357 Bymovirus Species 0.000 description 1
- 102100031102 C-C motif chemokine 4 Human genes 0.000 description 1
- 241000710011 Capillovirus Species 0.000 description 1
- 241000710175 Carlavirus Species 0.000 description 1
- 241000714207 Carmovirus Species 0.000 description 1
- 241000710173 Carnation latent virus Species 0.000 description 1
- 241000714206 Carnation mottle virus Species 0.000 description 1
- 241000723666 Carnation ringspot virus Species 0.000 description 1
- 241000969784 Carrot mottle virus Species 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 241001115395 Caulimoviridae Species 0.000 description 1
- 241000701459 Caulimovirus Species 0.000 description 1
- 241000156297 Chinese Rape Mosaic Virus Species 0.000 description 1
- 241000973027 Closteroviridae Species 0.000 description 1
- 241000710151 Closterovirus Species 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 241000701515 Commelina yellow mottle virus Species 0.000 description 1
- 241000723607 Comovirus Species 0.000 description 1
- 241000948358 Crinivirus Species 0.000 description 1
- 241000724252 Cucumber mosaic virus Species 0.000 description 1
- 241000724253 Cucumovirus Species 0.000 description 1
- 241000702461 Curtovirus Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000712467 Cytorhabdovirus Species 0.000 description 1
- 241000723672 Dianthovirus Species 0.000 description 1
- 241000723747 Enamovirus Species 0.000 description 1
- 241000723648 Fabavirus Species 0.000 description 1
- 241001302129 Fiji disease virus Species 0.000 description 1
- 241000702658 Fijivirus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000723722 Furovirus Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241001478515 Grapevine fleck virus Species 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- 241000724309 Hordeivirus Species 0.000 description 1
- 241001533403 Idaeovirus Species 0.000 description 1
- 241000724277 Ilarvirus Species 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 241001429356 Lettuce infectious yellows virus Species 0.000 description 1
- 241000191478 Lettuce necrotic yellows cytorhabdovirus Species 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 241000709757 Luteovirus Species 0.000 description 1
- 241001533339 Machlomovirus Species 0.000 description 1
- 241000710118 Maize chlorotic mottle virus Species 0.000 description 1
- 241000611254 Maize rayado fino virus Species 0.000 description 1
- 241000702489 Maize streak virus Species 0.000 description 1
- 241000442455 Maize white line mosaic virus Species 0.000 description 1
- 241000709759 Marafivirus Species 0.000 description 1
- 241000711513 Mononegavirales Species 0.000 description 1
- 101000777470 Mus musculus C-C motif chemokine 4 Proteins 0.000 description 1
- 241000723638 Nepovirus Species 0.000 description 1
- 108010077850 Nuclear Localization Signals Proteins 0.000 description 1
- 102220482008 Nuclear cap-binding protein subunit 2_T76R_mutation Human genes 0.000 description 1
- 241000712466 Nucleorhabdovirus Species 0.000 description 1
- 241001467023 Olive latent virus 2 Species 0.000 description 1
- 241000712894 Orthotospovirus Species 0.000 description 1
- 241000702633 Oryzavirus Species 0.000 description 1
- 241001112513 Ourmia melon virus Species 0.000 description 1
- 241000726026 Parsnip yellow fleck virus Species 0.000 description 1
- 241000710936 Partitiviridae Species 0.000 description 1
- 241001443531 Pea enation mosaic virus 1 Species 0.000 description 1
- 241000220691 Pelargonium zonate spot virus Species 0.000 description 1
- 241000723824 Pepper mild mottle virus Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000150350 Peribunyaviridae Species 0.000 description 1
- 241000609851 Petunia vein clearing virus Species 0.000 description 1
- 241001112770 Petuvirus Species 0.000 description 1
- 241000702656 Phytoreovirus Species 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 101900259239 Potato virus X Coat protein Proteins 0.000 description 1
- 241001474398 Potato yellow dwarf nucleorhabdovirus Species 0.000 description 1
- 241000710007 Potexvirus Species 0.000 description 1
- 241001533393 Potyviridae Species 0.000 description 1
- 241000710078 Potyvirus Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- 241000724648 Raspberry bushy dwarf virus Species 0.000 description 1
- 241000702247 Reoviridae Species 0.000 description 1
- 241000711931 Rhabdoviridae Species 0.000 description 1
- 101710097247 Ribulose bisphosphate carboxylase large chain Proteins 0.000 description 1
- 101710104360 Ribulose bisphosphate carboxylase large chain, chromosomal Proteins 0.000 description 1
- 241000144068 Rice ragged stunt virus Species 0.000 description 1
- 241000724205 Rice stripe tenuivirus Species 0.000 description 1
- 241000701507 Rice tungro bacilliform virus Species 0.000 description 1
- 241001492231 Rice tungro spherical virus Species 0.000 description 1
- 241000124072 Ryegrass mosaic virus Species 0.000 description 1
- 241001533356 Rymovirus Species 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 241000709666 Sequivirus Species 0.000 description 1
- 108010052160 Site-specific recombinase Proteins 0.000 description 1
- 241000710119 Sobemovirus Species 0.000 description 1
- 241001135883 Soil-borne wheat mosaic virus Species 0.000 description 1
- 241000710117 Southern bean mosaic virus Species 0.000 description 1
- 241000701529 Soybean chlorotic mottle virus Species 0.000 description 1
- 241000186983 Streptomyces avidinii Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000724318 Tenuivirus Species 0.000 description 1
- 241000723573 Tobacco rattle virus Species 0.000 description 1
- 241000723677 Tobacco ringspot virus Species 0.000 description 1
- 241000724291 Tobacco streak virus Species 0.000 description 1
- 241000723717 Tobravirus Species 0.000 description 1
- 241000710145 Tomato bushy stunt virus Species 0.000 description 1
- 241000016010 Tomato spotted wilt orthotospovirus Species 0.000 description 1
- 241001533336 Tombusviridae Species 0.000 description 1
- 241000710141 Tombusvirus Species 0.000 description 1
- 241000122134 Trichovirus Species 0.000 description 1
- 101000933110 Turnip vein-clearing virus Capsid protein Proteins 0.000 description 1
- 241000710155 Turnip yellow mosaic virus Species 0.000 description 1
- 241000710136 Tymovirus Species 0.000 description 1
- 241001533358 Umbravirus Species 0.000 description 1
- 241000709760 Waikavirus Species 0.000 description 1
- 235000000760 Wasabia japonica Nutrition 0.000 description 1
- 244000195452 Wasabia japonica Species 0.000 description 1
- 241001137667 White clover cryptic virus 1 Species 0.000 description 1
- 241001396914 White clover cryptic virus 2 Species 0.000 description 1
- 241000702661 Wound tumor virus Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011097 chromatography purification Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000001553 co-assembly Methods 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 241001492478 dsDNA viruses, no RNA stage Species 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 108010021843 fluorescent protein 583 Proteins 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 244000052637 human pathogen Species 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000005442 molecular electronic Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 108010054624 red fluorescent protein Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 102200111112 rs397514590 Human genes 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 241001147420 ssDNA viruses Species 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012385 systemic delivery Methods 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 241001336411 unassigned viruses Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 241000709655 unidentified tobacco necrosis virus Species 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/00041—Use of virus, viral particle or viral elements as a vector
- C12N2770/00043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/26011—Flexiviridae
- C12N2770/26023—Virus like particles [VLP]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05020311.6 | 2005-09-17 | ||
| EP05020311A EP1764414A1 (en) | 2005-09-17 | 2005-09-17 | Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification |
| PCT/EP2006/009029 WO2007031339A1 (en) | 2005-09-17 | 2006-09-15 | Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2621243A1 true CA2621243A1 (en) | 2007-03-22 |
Family
ID=35427929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002621243A Abandoned CA2621243A1 (en) | 2005-09-17 | 2006-09-15 | Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20090062514A1 (enExample) |
| EP (2) | EP1764414A1 (enExample) |
| JP (1) | JP2009507503A (enExample) |
| KR (1) | KR20080065613A (enExample) |
| CN (1) | CN101268192A (enExample) |
| AU (1) | AU2006291435A1 (enExample) |
| CA (1) | CA2621243A1 (enExample) |
| WO (1) | WO2007031339A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2215223B1 (en) * | 2007-10-31 | 2013-05-01 | Precision Biosciences, Inc. | Rationally-designed single-chain meganucleases with non-palindromic recognition sequences |
| EP2500035A1 (en) | 2011-03-15 | 2012-09-19 | Icon Genetics GmbH | Pharmaceutical formulation containing immunglobulin |
| US10117923B2 (en) | 2012-11-07 | 2018-11-06 | Vacplanta Limited | Production of an immunogen using a plant virus |
| WO2014074509A1 (en) * | 2012-11-07 | 2014-05-15 | Vacplanta Limted | Production of an immunogen using a plant virus |
| EP2964032A4 (en) * | 2013-03-05 | 2016-11-23 | Shifa Biomedical | COMPOSITIONS AND METHOD FOR THE PRODUCTION OF VIRUSELIC PARTICLES |
| EP2777711A1 (en) | 2013-03-11 | 2014-09-17 | Icon Genetics GmbH | Her2/Neu cancer vaccine |
| EP3085774A1 (en) * | 2015-04-20 | 2016-10-26 | QIAGEN GmbH | Method for concentrating, purifying and/or isolating virus particles |
| CA2985490A1 (en) * | 2015-06-17 | 2016-12-22 | E.I. Du Pont De Nemours And Company | Plant regulatory elements and methods of use thereof |
| WO2017020570A1 (en) * | 2015-08-06 | 2017-02-09 | Medigen Biotechnology Corp. | Virus-like particle vaccines |
| WO2017051434A1 (en) * | 2015-09-22 | 2017-03-30 | Indian Institute Of Science | Chimeric plant based virus-like-particles, and uses thereof |
| CN106566842B (zh) * | 2016-07-18 | 2019-04-19 | 湖南农业大学 | 一种蛋白或多肽的植物表达载体及其应用 |
| CN108956983B (zh) * | 2018-05-07 | 2021-05-07 | 西北工业大学 | 一种以烟草花叶病毒为模板的微传感器可控阵列化制备方法 |
| KR102714626B1 (ko) * | 2018-06-12 | 2024-10-08 | 케이바이오 홀딩스 리미티드 | 바이러스 및 항원 정제 및 접합 |
| US11690907B2 (en) | 2018-06-12 | 2023-07-04 | Kbio Holdings Limited | Vaccines formed by virus and antigen conjugation |
| US11529413B2 (en) | 2018-06-12 | 2022-12-20 | Kbio Holdings Limited | Virus and antigen purification and conjugation |
| US11696948B2 (en) | 2018-06-12 | 2023-07-11 | Kbio Holdings Limited | Vaccines formed by virus and antigen conjugation |
| CN118684781A (zh) * | 2023-03-21 | 2024-09-24 | 深圳赫兹生命科学技术有限公司 | GnRH-VLP重组去势疫苗及其制备方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5466788A (en) | 1985-03-07 | 1995-11-14 | Mycogen Plant Science, Inc. | Subgenomic promoter |
| US6660500B2 (en) * | 1988-02-26 | 2003-12-09 | Large Scale Biology Corporation | Production of peptides in plants as viral coat protein fusions |
| US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
| US5977438A (en) | 1988-02-26 | 1999-11-02 | Biosource Technologies, Inc. | Production of peptides in plants as viral coat protein fusions |
| IT1249603B (it) | 1991-02-21 | 1995-03-09 | Cremona Angelo & Figlio | Stazione di taglio automatizzata per tranciati di legno |
| JP2880024B2 (ja) * | 1992-03-31 | 1999-04-05 | 鐘紡株式会社 | 植物ウィルスベクター及びプラスミド並びに外来遺伝子を植物中で発現させる方法 |
| EP1162267A3 (en) * | 1992-03-31 | 2002-12-18 | Kanebo Limited | Plant virus vector, plasmid, process for expression of foreign gene and process for obtaining foreign gene product |
| JPH06169789A (ja) * | 1992-12-08 | 1994-06-21 | Kanebo Ltd | 外来遺伝子産物の取得方法 |
| US5491076A (en) | 1993-11-01 | 1996-02-13 | The Texas A&M University System | Expression of foreign genes using a replicating polyprotein producing virus vector |
| US5670353A (en) | 1994-08-25 | 1997-09-23 | Mycogen Plant Science, Inc. | Subgenomic promoter |
| DE10049587A1 (de) | 2000-10-06 | 2002-05-02 | Icon Genetics Ag | Vektorsystem für Pflanzen |
| JP3936661B2 (ja) * | 2001-01-18 | 2007-06-27 | アイコン・ジェネティクス，インコーポレイテッド | タンパク質を検出または精製するためのウィルスの使用 |
| EP2184363B1 (en) | 2003-11-10 | 2013-08-28 | Icon Genetics GmbH | RNA virus-derived plant expression system |
| EP1686176A1 (en) | 2005-01-28 | 2006-08-02 | Icon Genetics AG | Production of antibodies in plants with plus-sense single-stranded viral RNA vectors |
-
2005
- 2005-09-17 EP EP05020311A patent/EP1764414A1/en not_active Withdrawn
-
2006
- 2006-09-15 AU AU2006291435A patent/AU2006291435A1/en not_active Abandoned
- 2006-09-15 WO PCT/EP2006/009029 patent/WO2007031339A1/en not_active Ceased
- 2006-09-15 US US12/067,109 patent/US20090062514A1/en not_active Abandoned
- 2006-09-15 CA CA002621243A patent/CA2621243A1/en not_active Abandoned
- 2006-09-15 EP EP06792110A patent/EP1926819B1/en active Active
- 2006-09-15 JP JP2008530428A patent/JP2009507503A/ja active Pending
- 2006-09-15 CN CNA2006800340346A patent/CN101268192A/zh active Pending
- 2006-09-15 KR KR1020087008611A patent/KR20080065613A/ko not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007031339A1 (en) | 2007-03-22 |
| EP1926819A1 (en) | 2008-06-04 |
| EP1764414A1 (en) | 2007-03-21 |
| EP1926819B1 (en) | 2013-01-16 |
| AU2006291435A1 (en) | 2007-03-22 |
| KR20080065613A (ko) | 2008-07-14 |
| AU2006291435A8 (en) | 2008-05-15 |
| CN101268192A (zh) | 2008-09-17 |
| US20090062514A1 (en) | 2009-03-05 |
| JP2009507503A (ja) | 2009-02-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1926819B1 (en) | Plant viral particles comprising a plurality of fusion proteins consisting of a plant viral coat protein, a peptide linker and a recombinant protein and use of such plant viral particles for protein purification | |
| Lindbo | TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector | |
| Peyret et al. | When plant virology met Agrobacterium: the rise of the deconstructed clones | |
| Lico et al. | Viral vectors for production of recombinant proteins in plants | |
| Komarova et al. | Transient expression systems for plant-derived biopharmaceuticals | |
| Sainsbury et al. | Cowpea mosaic virus: the plant virus–based biotechnology workhorse | |
| Huang et al. | High‐level rapid production of full‐size monoclonal antibodies in plants by a single‐vector DNA replicon system | |
| RU2539793C2 (ru) | Системы экспрессии белка | |
| US20150315604A1 (en) | Productions of hetero-oligomeric proteins in plants | |
| CA2824152C (en) | Method and system for producing a heterologous polypeptide in a nicotiana tabacum plant | |
| Wagner et al. | Plant virus expression systems for transient production of recombinant allergens in Nicotiana benthamiana | |
| JP7164894B2 (ja) | アフリカ豚熱の診断のための抗原生産用組み換えベクター及びその使用 | |
| Lacomme et al. | Plant viruses | |
| Majer et al. | A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein | |
| CA2621466A1 (en) | Modified tobacco mosaic virus particles as scaffolds for display of protein antigens for vaccine applications | |
| JP3936661B2 (ja) | タンパク質を検出または精製するためのウィルスの使用 | |
| Soleimanizadeh et al. | Enhanced expression and purification of anti-VEGF nanobody in cucurbit plants | |
| AU2002243614A1 (en) | Using viruses to detect or purify proteins | |
| Zhang et al. | Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana | |
| Shokrian Hajibehzad et al. | High-level transient expression of the N-terminal domain of IpaD from Shigella dysenteriae in four plant species transformed with different construct configurations | |
| Hajibehzad et al. | High-level transient expression of the N-terminal domain of IpaD from Shigella dysenteriae in four plant species transformed with different construct configurations | |
| Rosenthal | Improving expression vectors for recombinant protein production in plants | |
| Andrzej | 3 Green Way of Biomedicine–How to Force Plants to Produce New Important Proteins |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |
Effective date: 20130917 |