CN101263861B - Preparation animal protolysate - Google Patents
Preparation animal protolysate Download PDFInfo
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- CN101263861B CN101263861B CN2008100735731A CN200810073573A CN101263861B CN 101263861 B CN101263861 B CN 101263861B CN 2008100735731 A CN2008100735731 A CN 2008100735731A CN 200810073573 A CN200810073573 A CN 200810073573A CN 101263861 B CN101263861 B CN 101263861B
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Abstract
The invention discloses a preparation method of hydrolyzed animal protein, which is characterized in that: animal protein with weight account of 10 to 90 and egg protein with weight account of 10 to 90 are mixed in a vessel before stirred evenly through adding water 0.5 to 5 times amount, boiled and cooled; then proteinase or pamcreatin is added for hydrolysis, thereby getting hydrolysate; the hydrolysate is filtered to get the filtrate which is boiled before frozen to get supernatant; concentration and filtering is made to get hydrolyzed protein concentrate solution which is atomized and dried to get hydrolyzed animal protein. Total nitrogen content of the hydrolyzed animal protein can reach more than 12%, rate of amino acid reaches more than 8%. The invention can be used as clinical medication or additive of nutrition food with properties of high nutrition, easy absorption and easy popularization. The invention can be clinically used for hypoproteinemia and malnutrition and systemic failure caused by each disease and also can be used for burn, fracture and poor wound healing for postoperative wound. The hydrolyzed animal protein has the advantages of obvious therapeutic effect, good taste, convenient use, big market requirement and remarkable economic benefit.
Description
Technical field
The present invention relates to a kind of method of protein hydrolysis, especially a kind of preparation method of animal hydrolyzed protein.
Background technology
At present, mainly be to have adopted three kinds of processes about the proteolysis technology both at home and abroad: acid-hydrolysis method, alkali hydrolysis method and enzyme hydrolysis method.Wherein acid-hydrolysis method and alkali hydrolysis method belong to chemical hydrolysis, and enzyme hydrolysis method belongs to the biological enzyme hydrolysis method.Acid-hydrolysis method strong acid commonly used is sulfuric acid or hydrochloric acid hydrolysis, is 6 mol in the molar concentration of acid, and hydrolysis is more than 20 hours under the condition that high temperature is 110 ℃, and the shortcoming of the method is that hydrolysis time is long, the acidic materials etching apparatus; After the acid hydrolysis, wherein asparagine and GLU are aspartic acid and glutamic acid by complete hydrolysis respectively, and tryptophan is then destroyed fully; Serine and threonine are by partial hydrolysis, and loss is 10% and 5%, and the total nitrogen rate only is 8～9%, and reaction condition needs high temperature, strong acid, and environmental pollution is serious.Alkali hydrolysis method generally adopts NaOH or potassium hydroxide, long-time hydrolysis, most amino acid can suffer the destruction of distinct program, destroyed such as serine, threonine, arginine and cysteine, other amino acid racemization, thereby the nutritive value of its product enzymolysis liquid is reduced, and economic benefit reduces.
And the reaction condition gentleness of enzyme hydrolysis method, it is less that energy consumes, and amino acid is not destroyed, and does not produce racemization yet, and the efficient height, has incomparable advantage in the reservation of nutritional labeling.The approach of enzyme source has multiple in the enzyme hydrolysis, can utilize the various hydrolase pair cell tissues that self exist in the biological tissue to decompose, can also decompose by adding the exogenous enzymes pair cell tissue that is fit to reaction system, and the hydrolase kind that self exists in the cell tissue is a lot, vigor has nothing in common with each other, but also can be subjected to the influence of other speciality in the body, exist the self-dissolving hydrolytic enzyme activities to lose easily in the body.And, utilize the self-dissolving hydrolase aminosal that exists in the body, it is long to exist the reaction time, and efficient is not high, and productive rate is low, and shortcoming such as the easy inactivation of self-dissolving hydrolase.Therefore, the hydrolase that utilize animal body or grain self to exist is a ten minutes finite sum difficulty to its quick hydrolysis.
Patent has the preparation method of relevant hydrolytic soya bean protein liquid beverage, the method for commercial production of compound amino acid powder from protein hydrolyzate etc. that kind more than 100 is arranged at present.It is to extract albumen and porcine haemoglobin etc. with soybean that many patents are arranged.
Summary of the invention
The purpose of this invention is to provide a kind of method of hydrolyzing protein through enzyme, and use this method to produce and to regenerate, to strengthen hematopoietic function for the histocyte of promotion that has of clinical and food applications, improve immunity, the oral hydrolyzed protein that promotion is grown, human body absorbs easily.
The prescription of our research mainly extracts effective ingredient by beef or crocodile meat etc., soybean through scientific method.Beef, crocodile meat contain rich in protein, can improve body resistance against diseases, to the people of grow and perform the operation back, aftercare replenish lose blood, aspect such as repair tissue is suitable especially.The traditional Chinese medical science thinks that beef has tonifying middle-Jiao and Qi, nourishes the effect of taste, strong muscles and bones, the breath wind that reduces phlegm, the saliva that quenches the thirst.Be applicable to the sinking of qi of middle-jiao, the body void of breathing hard, yellow dizzy people is edible for soreness of bones and muscles, anaemia prolonged illness and face.Containing abundant vitamin A, B, D, E and multiple human body in the soybean can not synthesize but essential amino acid.Normal food soybean can make skin delicacy, pale, moist, effectively prevents the appearance of freckle and wrinkle, and the high content of protein matter in the soybean can nutrition skin hair, makes human body plentiful solid, and hair is pitch-black glossy, and is agerasia.
The technical solution used in the present invention is:
Animal protein 10～90 weight portions, egg protein 10～90 weight portions are placed in the container and mix, add 0.5～5 times of amount of water, stir, boiled 30 minutes, the water-bath cooling is to 30～50 ℃, add protease or pancreatin, 30～45 ℃ of temperature, hydrolysis is 3 hours under pH4.0～8.0 conditions, gets hydrolyzate; Hydrolyzate is filtered, and filtrate is boiled, in 0～5 ℃ of refrigeration 5～12 hours, leaching supernatant; Concentrate, filter, get the protein hydrolysate concentrate; Spray-drying gets the animal hydrolyzed protein powder.
Described animal protein adopts beef or crocodile meat, and egg albumen adopts egg.
The protease addition is 0.3～0.6% of a material quantity; The addition of pancreatin is 30～60% of a material quantity.
It is 1.05～1.15 that 80 ℃ of filtrates are evaporated to proportion.
The protein hydrolysate powder is as clinical application or nutraceutical additive.
The present invention adopts the preparation of zymoprotein Hydrolyze method with animal protein, egg albumen, scientific and technical personnel through my company make great efforts to explore, research, repeated tests, finally work out employing protease, obtain 17 kinds or 18 seed amino acids after the hydrolysis, wherein contain trace elements such as 8 kinds of essential amino acids (tryptophan, lysine, leucine, isoleucine, threonine, methionine, phenylalanine, valine), small peptide, sugar and iron, zinc, calcium.See following testing result:
Testing result
The high nutrition of animal hydrolyzed protein of my company development, easily absorb, promote easily, be used for malnutrition, whole body depletion due to Hypoproteinemia and the various diseases clinically, it is bad also to can be used for burn, fracture and postoperative wound healing.Have the regeneration of the histocyte of promotion, strengthen hematopoietic function, improve the flag state, improve immunity, promote to grow.The white blood cell that is widely used in tumor patient radiotherapy, chemotherapy initiation reduces, the stream of people and women's postpartum anemia, in poor health, the childhood dystrophy that the elderly's hypoimmunity and a variety of causes cause, poor appetite etc., various symptoms to the Hypoproteinemia initiation, can help the patient to tide over the critical course of disease, correct negative nitrogen balance, but and the tube feed administration, improve tumor patient Abwehrkraft des Koepers and survival rate.Reach obvious curative effects.And mouthfeel is good, taking convenience, the huge market demand, remarkable in economical benefits.
The animal hydrolyzed protein that adopts the inventive method to produce can add the medication that various pharmaceutic adjuvants are made clinical various formulations, also can be used as the nutraceutical additive and uses.
The specific embodiment
Embodiment 1:
Beef 30kg, egg 70kg (shell breaking) are mixed in container, add water 150kg, stir, boiled 30 minutes, the water-bath cooling to 30 ℃, adds proteinase-10 .6kg, and market is on sale, 30 ℃ of temperature,, get hydrolyzate with hydrolysis under the ammoniacal liquor adjusting pH5.0 condition 3 hours; Hydrolyzate is filtered, and filter residue discards, and filtrate is boiled, in 0～5 ℃ of refrigeration 5 hours, leaching supernatant.Filter, get the animal hydrolyzed protein concentrate.Adopt spray-drying: 165～170 ℃ of EATs, spray-drying under 100～105 ℃ of conditions of leaving air temp gets the white protein powder.5/ bag, 10/ bag, 15/ bag of direct packaging is packaged into the powder finished product.
Embodiment 2:
Beef 40kg, egg 60kg (shell breaking) are mixed in container, add water 300kg, stir, boiled 30 minutes, the water-bath cooling to 45 ℃, adds proteinase-10 .3kg, 45 ℃ of temperature, with hydrolysis under the ammoniacal liquor adjusting pH8.0 condition 3 hours, gets hydrolyzate; Hydrolyzate is filtered, and filter residue discards, and filtrate is boiled, in 0～5 ℃ of refrigeration 5 hours, leaching supernatant.Spray-drying: 165～170 ℃ of EATs, spray-drying under 100～105 ℃ of conditions of leaving air temp gets the animal hydrolyzed protein powder.
The animal hydrolyzed protein powder.Become capsule with the 0# capsule subpackage.With 40/bottle, 100/bottle, packed products gets final product.
Embodiment 3:
Crocodile meat 10kg, egg 90kg (shell breaking) are mixed in container, add water 300kg, stir, boiled 30 minutes, the water-bath cooling, to 40 ℃, add pancreatin 30kg (pig pancreatin or animal pancreatin, commercially available prod), 38～40 ℃ of temperature, with hydrolysis under the ammoniacal liquor adjusting pH6.5 condition 3 hours, get hydrolyzate; Hydrolyzate is filtered, and filter residue discards, and filtrate is boiled, in 0～5 ℃ of refrigeration 12 hours, leaching supernatant.Filter spray-drying: 165～170 ℃ of EATs, spray-drying under 100～105 ℃ of conditions of leaving air temp gets white animal hydrolyzed protein powder.
Animal hydrolyzed protein powder 20%, cane sugar powder 80% add an amount of medicinal adjuvant of mixing and make particle, at 50～80 ℃ of whole grains in dry back, packing 10g/ bag, 15g/ bag.Be packaged into the granule finished product.
Embodiment 4:
Beef 20kg, egg 80kg (shell breaking) are mixed in container, add water 300kg, stir, boiled 30 minutes, the water-bath cooling to 40 ℃, adds pancreatin 40kg, 38～40 ℃ of temperature, with hydrolysis under the ammoniacal liquor adjusting pH6.5 condition 3 hours, gets hydrolyzate; Hydrolyzate is filtered, and filter residue discards, and filtrate is boiled, in 0～5 ℃ of refrigeration 5 hours, leaching supernatant.Filter spray-drying: 165～170 ℃ of EATs, spray-drying under 100～105 ℃ of conditions of leaving air temp gets white animal hydrolyzed protein powder.
The about 20kg of albumen powder adds entry 1000kg adding appropriate amount of auxiliary materials anticorrisive agent and is mixed with liquid preparation, through being distributed into specifications such as 30ml, 100ml.Be packaged into the oral liquid finished product.
Embodiment 5:
Beef 90kg, egg 10kg (shell breaking) are mixed in container, add water 500kg, stir, boiled 30 minutes, the water-bath cooling to 40 ℃, adds pancreatin 50kg, 38～40 ℃ of temperature, with hydrolysis under the ammoniacal liquor adjusting pH6.5 condition 3 hours, gets hydrolyzate; Hydrolyzate is filtered, and filter residue discards, and filtrate is boiled, in 0～5 ℃ of refrigeration 8 hours, leaching supernatant.Filter spray-drying: 165～170 ℃ of EATs, spray-drying under 100～105 ℃ of conditions of leaving air temp gets white animal hydrolyzed protein powder.
Protein hydrolysate powder and carboxyrnethyl starch sodium, heating, fusing, 73 ± 2 ℃ of insulation degassings.Select suitable water dropper, splash in the dimeticone of cooling, after the moulding dripping pill is taken out, remove surperficial dimeticone, the bag film-coating.By packing specification pack the pill finished product.
Claims (3)
1. the preparation method of an animal hydrolyzed protein is characterized in that:
Beef or crocodile meat 10～90 weight portions, egg 10～90 weight portions are placed in the container and mix, add 0.5～5 times of amount of water, stir, boiled 30 minutes, the water-bath cooling is to 30～50 ℃, add protease or pancreatin, 30～45 ℃ of temperature, hydrolysis is 3 hours under pH4.0～8.0 conditions, gets hydrolyzate; Hydrolyzate is filtered, and filtrate is boiled, in 0～5 ℃ of refrigeration 5～12 hours, leaching supernatant; Concentrate, filter, get the protein hydrolysate concentrate; Spray-drying gets the animal hydrolyzed protein powder;
The addition of described pancreatin is 30～60% of a material quantity;
The addition of described protease is 0.3～1% of a material quantity.
2. the preparation method of animal hydrolyzed protein according to claim 1, it is characterized in that: described pancreatin refers to the pig pancreatin.
3. the preparation method of animal hydrolyzed protein according to claim 1, it is characterized in that: described pancreatin refers to the animal pancreatin.
Priority Applications (1)
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| CN2008100735731A CN101263861B (en) | 2008-05-08 | 2008-05-08 | Preparation animal protolysate |
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| CN2008100735731A CN101263861B (en) | 2008-05-08 | 2008-05-08 | Preparation animal protolysate |
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| CN101263861A CN101263861A (en) | 2008-09-17 |
| CN101263861B true CN101263861B (en) | 2011-03-23 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103255190A (en) * | 2013-05-30 | 2013-08-21 | 威海康博尔生物药业有限公司 | Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme |
| CN107279455A (en) * | 2017-08-08 | 2017-10-24 | 天津市虎豹调味品酿造有限公司 | The preparation method of cod bone protein hydrolysate |
| CN107772046A (en) * | 2017-11-22 | 2018-03-09 | 广西德澳新农业科技有限公司 | A kind of processing and treating method of bamboo rat tail protein hydrolysate |
| CN107928386A (en) * | 2017-12-04 | 2018-04-20 | 梅瑞 | A kind of amino acid manufacturing method and apparatus |
| CN113667710A (en) * | 2021-08-31 | 2021-11-19 | 北京化工大学 | Chicken-derived protein hydrolysate for inhibiting cancer cell proliferation and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1479055A1 (en) * | 1987-07-27 | 1989-05-15 | Московский городской научно-исследовательский институт скорой помощи им.Н.В.Склифосовского | Method of producing nutritive mixture for intraintestinal tube feeding |
| EP0325986A2 (en) * | 1988-01-28 | 1989-08-02 | Miles Inc. | Enzymatic hydrolysis of proteins |
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN1403583A (en) * | 2002-07-12 | 2003-03-19 | 成都心友生物技术有限责任公司 | Prepn of natural active protein and peptide |
-
2008
- 2008-05-08 CN CN2008100735731A patent/CN101263861B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1479055A1 (en) * | 1987-07-27 | 1989-05-15 | Московский городской научно-исследовательский институт скорой помощи им.Н.В.Склифосовского | Method of producing nutritive mixture for intraintestinal tube feeding |
| EP0325986A2 (en) * | 1988-01-28 | 1989-08-02 | Miles Inc. | Enzymatic hydrolysis of proteins |
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN1403583A (en) * | 2002-07-12 | 2003-03-19 | 成都心友生物技术有限责任公司 | Prepn of natural active protein and peptide |
Non-Patent Citations (4)
| Title |
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| 毛善勇等.复合酶水解牛肉的研究.肉类研究 9.2004,(9),16-18. |
| 毛善勇等.复合酶水解牛肉的研究.肉类研究 9.2004,(9),16-18. * |
| 舒夏娃等.动物蛋白制备生物活性肽酶解工艺的研究进展.饲料与畜牧 2.2006,(2),38-41. |
| 舒夏娃等.动物蛋白制备生物活性肽酶解工艺的研究进展.饲料与畜牧 2.2006,(2),38-41. * |
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