CN101518295B - Method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons - Google Patents
Method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons Download PDFInfo
- Publication number
- CN101518295B CN101518295B CN 200810059760 CN200810059760A CN101518295B CN 101518295 B CN101518295 B CN 101518295B CN 200810059760 CN200810059760 CN 200810059760 CN 200810059760 A CN200810059760 A CN 200810059760A CN 101518295 B CN101518295 B CN 101518295B
- Authority
- CN
- China
- Prior art keywords
- molecular weight
- whey
- dalton
- powder
- small peptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons belongs to the technical field of processing dairy products. The main production process thereof is as follows: enzymatic hydrolysis, embedding and homogenization are firstly carried out on concentrated powder (WPC80 powder) containing 80 percent of whey protein, then the powder is prepared by spray drying, and the product can be used as functional ingredients for infant milk powder. The method adopts trypsin for enzymatic hydrolysis, the degree of hydrolysis achieves 20-21 percent, and then hydrophilic-hydrophobic wall materials of 5-10 percent of beta-cyclodextrin and 5-8 percent of lecithin are adopted, so the method can not only bury the bitterness, but also maintain the biological activities of anti-protein allergy and improve the immunity of the whey small peptides with the molecular weight of 1000 daltons to the maximum extent. The method can be applied to the infant formula milk powder and have good effects of improving the ability of anti-protein allergy of the infant milk powder and improving the immunity.
Description
Technical field
A kind of molecular weight that is rich in is 1000 dalton's whey small peptides powder preparation methods, belongs to technical field of processing dairy products.
Background technology
Human polypeptide's Material Source mainly is two aspects in proteinaceous nutrient, the one, and food protein in digestion process produces polypeptide, absorbed by health, the 2nd, cells in vivo utilizes the degradation product amino acid of protein directly synthetic.If owing to reasons such as nutrition, digestion, diseases, peptide material lacks in the body, the health of human body is affected, for example: immunocompetence reduction, indigestion, metabolic disorder, dysplasia etc.Therefore, scientist's suggestion for somatogenic need, after the basic nutrition that has satisfied human body is taken in, suitably replenishes polypeptide nutrition, is of great benefit to for healthy and rehabilitation.Studies show that, no matter lactalbumin is the physiologically active function, or digestion and absorption function, all greatly is better than casein, belongs to the full price high-quality protein, the height of tiring.Whey small peptides can not only provide people's bulk-growth, grow required nutriment, and has unique biological function.
At present both at home and abroad the patent of invention aspect functional whey peptides is less, mainly lay particular emphasis on lactalbumin technique with and application and development in milk powder.Chinese patent CN1409974A has reported the antifatigue effect of the concentrated powder of a kind of undenatured whey protein, is the product of liquid condition, is unfavorable for storing and transportation, and is inconvenient as additive application.Chinese patent CN1392773A has reported a kind of lactalbumin emulsion preparation method, whey protein is sneaked into intensifying method and the composition of dairy products, the homogeneous mixture or the emulsion that comprise whey protein and rare cream comprising formation, and the homogeneous mixture of hybrid packet whey protein-containing and rare cream or emulsion, and this homogeneous mixture or emulsion sneaked into dairy products, this patent is only suitable in wet production milk powder.Chinese patent CN1409974 patent of invention discloses the concentrated powder of a kind of undenatured whey protein as the application of preparation anti-fatigue health-product containing, be included in low altitude area and high altitude localities and make the direct oral beverage that is capsule of antifatigue without auxiliary material, or the oral beverage that mixes with liquid, the concentrated powder of the undenatured whey protein of its powdery and drinking water or fruit juice or sour milk or cold milk or cold shot slurry or cold oatmeal and analog thereof directly mix oral drinking without stirring down being lower than 40 ℃, directly utilize lactalbumin, its biological function is further brought into play.Chinese patent CN1386783A has reported a kind of heat resistant type microcapsules dalton HA preparation technology, has wherein comprised to adopt two stage embedding techniqueses, and a kind of new embedding method is provided, and does not compare with some functional little peptides.Chinese patent CN1419936A has reported the method for hemoglobin microcapsule blood substitute, mainly adopts special embedding method, then carries out homogeneous etc.
In sum, in existing background technology, do not relate to the to a certain extent preparation methods such as rear microencapsulation, spray-drying of hydrolyzed whey protein, do not relate to the aspects such as product application.
Summary of the invention
The present invention is to provide a kind of take the WPC80 powder as primary raw material, adopt trypsase enzymolysis under fixing condition, be degraded to 20～21% and obtain molecular weight for being rich in 1000 dalton's whey small peptides, then adopt the wall material beta-schardinger dextrin-of close and distant combination and lecithin with the bitter taste embedding, then carrying out spray-drying, to make molecular weight be 1000 dalton's whey small peptides powder, can be used as antiallergy and improve one of formula milk batching of immunity, improve Chinese physique.The invention solves the outstanding difficult problem of bitter taste behind the WPC80 powder degree of hydrolysis height, for the simulation breast milk composition provides a kind of new method.
Technical solution
A kind of molecular weight that is rich in is that 1000 dalton's whey small peptides powder preparation methods are: 1) enzymolysis: first PURE WHEY is added water (adding 50mL distilled water by the 3.0g whey powder calculates) stirring and dissolving, be preheated to 60 ℃, hydro-oxidation sodium or hydrochloric acid solution transfer to pH7.0, then add the required trypsase (ratio of enzyme/substrate is 3%) of hydrolysis, enzymolysis 5h in 60 ℃ of waters bath with thermostatic control; 2) microencapsulation: take enzymolysis liquid as core, adopt wall material 5～10% beta-schardinger dextrin-s of close and distant combination and 5～8% lecithin with the bitter taste embedding, its ratio is 70: 15: 15, and the rotating speed of embedding high speed dispersion is 20000r/min; 3) spray-drying, drying condition are 190 ℃ of EATs, and leaving air temp is 95 ℃, and material flow is 1.35L/h.
The invention provides a kind of take the WPC80 powder as primary raw material, be used in conjunction with trypsin hydrolysis and obtain molecular weight 1000 daltonian whey small peptides, adopt 5～10% beta-schardinger dextrin-s and 5～8% lecithin microencapsulation technologies to process also and adopt spray-dired technique under the certain condition.
Beneficial effect of the present invention
Compare with the application that the WPC80 powder is traditional, it is 1000 dalton's whey small peptides that the present invention can obtain molecular weight, and the acquisition condition control of lactalbumin than the active hydrolysate active peptides of homogeneous is provided.
Compare with the WPC80 powder, molecular weight of the present invention is that 1000 dalton's whey small peptides have the functions such as anti-albumen is irritated, enhancing immunity.
Compare with traditional microencapsulation effect, adopt wall material 5～10% beta-schardinger dextrin-s and 5～8% lecithin of close and distant combination both bitter taste can be buried, can use up also that to keep to greatest extent molecular weight be the BA of 1000 dalton's whey small peptides.
Compare with traditional milk powder, molecular weight of the present invention is that 1000 dalton's whey small peptides are applied in the baby formula milk powder, can improve the functions such as the anti-albumen allergy of infant, immunocompetence.
Comprehensive and opinion is compared with background technology, the present invention is by the enzymolysis to lactalbumin, obtaining molecular weight is 1000 daltonian bitter taste whey small peptides, then by microencapsulation technology with the bitter taste embedding, be applied in the formula milk, can improve the effects such as anti-albumen allergy of baby formula milk powder.
The specific embodiment
Embodiment 1: the preparation of molecular weight 1000 dalton's whey small peptides powder is got the 30kg whey and is dissolved in the 50L water, stir, be preheating to 60 ℃, hydro-oxidation sodium or hydrochloric acid solution transfer to pH7.0, then add the required trypsase 1.5kg of hydrolysis, enzymolysis 5h in 60 ℃ of waters bath with thermostatic control, take enzymolysis liquid as core, wall material 5～10% beta-schardinger dextrin-s of the close and distant combination of adding employing and 5～8% lecithin are with the bitter taste embedding, its ratio is 70: 15: 15, the rotating speed of embedding high speed dispersion is 20000r/min, disperses 10min; Spray-drying, drying condition are 190 ℃ of EATs, and leaving air temp is 95 ℃, and material flow is 1.35L/h, cooling, packing, storage.
Claims (3)
1. a molecular weight is 1000 dalton's whey peptides preparation methods, it is characterized in that:
(1) the 3.0g PURE WHEY is dissolved in the 50mL distilled water, stirs, then be preheated to 60 ℃, hydro-oxidation sodium or hydrochloric acid solution transfer to pH7.0, add 0.09g trypsase, enzymolysis 5h in 60 ℃ of waters bath with thermostatic control, making molecular weight is 1000 dalton's whey peptides enzymolysis liquids;
(2) cycloheptaamylose and lecithin are dissolved in respectively form 5～10% concentration and 5～8% concentration solution in 60～70 ℃ of hot water;
(3) be cooled to 30～35 ℃;
(4) getting the above-mentioned 700mL molecular weight for preparing is 1000 dalton's whey peptides solution, adds 150mL cycloheptaamylose solution and 150mL lecithin soln, stirs 20min, fully dissolves each other;
(5) high speed dispersion, 10000r/min processes 10min;
(6) disperse rear emulsion to carry out spray-drying, drying condition is 190 ℃ of EATs, and leaving air temp is 95 ℃, and material flow is 1.35L/h.
2. a kind of molecular weight according to claim 1 is 1000 dalton's whey peptides preparation methods, it is characterized in that: after molecular weight product was the embedding of 1000 dalton's whey peptides process, embedding rate was more than 80%.
3. 1000 dalton's whey peptides powder purposes according to claim 1 is characterized in that: be used for dairy products, health products, medicine, feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810059760 CN101518295B (en) | 2008-02-25 | 2008-02-25 | Method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810059760 CN101518295B (en) | 2008-02-25 | 2008-02-25 | Method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101518295A CN101518295A (en) | 2009-09-02 |
| CN101518295B true CN101518295B (en) | 2013-03-06 |
Family
ID=41079258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810059760 Expired - Fee Related CN101518295B (en) | 2008-02-25 | 2008-02-25 | Method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101518295B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102251001B (en) * | 2011-06-22 | 2013-10-30 | 华南理工大学 | Preparation method of vegetable protein antioxidant |
| CN103271241B (en) * | 2013-04-27 | 2014-07-16 | 深圳安佑康牧科技有限公司 | Piglet forage ingredient and its preparation method and use |
| CN104757138B (en) * | 2015-04-28 | 2017-09-19 | 东北农业大学 | A kind of preparation and its method using prebiotics and WPC as the microencapsulation lactalbumin hydrolysate of wall material |
| CN105476012B (en) * | 2015-12-24 | 2019-04-05 | 中健医用食品有限公司 | A kind of special medicine purposes formula food and preparation method thereof |
| CN106858613B (en) * | 2017-03-08 | 2020-09-04 | 云南农业大学 | Compound spicy xyloglucan polypeptide-amino acid buccal tablet and preparation method thereof |
| CN107858394B (en) * | 2017-12-25 | 2021-09-07 | 内蒙古蒙牛乳业（集团）股份有限公司 | Method for preparing lactalbumin hydrolysate |
| CN111543578A (en) * | 2020-05-18 | 2020-08-18 | 济南大学 | Method for embedding sesame protein peptide peculiar smell by using instant coffee powder and resistant dextrin |
| CN112293635B (en) * | 2020-11-07 | 2023-07-04 | 润科生物工程（福建）有限公司 | Spray drying process of instant chlorella protein peptide powder and application of instant chlorella protein peptide powder in solid beverage |
| CN113383854A (en) * | 2021-04-09 | 2021-09-14 | 浙江科技学院 | Method for producing soybean protein isolate and whey small peptide extrusion modified product |
| CN113892655A (en) * | 2021-10-12 | 2022-01-07 | 长春大学 | Method for preparing digestion-free whey protein by taking whey protein powder as raw material |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854305A (en) * | 2005-04-26 | 2006-11-01 | 李长青 | Production of brain-tonifying nutrient oyster peptide |
| CN1994107A (en) * | 2005-12-31 | 2007-07-11 | 大连玉璘企业集团有限公司 | Method for preparing polypeptide nutrient |
-
2008
- 2008-02-25 CN CN 200810059760 patent/CN101518295B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854305A (en) * | 2005-04-26 | 2006-11-01 | 李长青 | Production of brain-tonifying nutrient oyster peptide |
| CN1994107A (en) * | 2005-12-31 | 2007-07-11 | 大连玉璘企业集团有限公司 | Method for preparing polypeptide nutrient |
Non-Patent Citations (2)
| Title |
|---|
| 包怡红.乳清多肽及其发酵制品的研究.《全国硕士论文数据库》.2002,全文. * |
| 梁玉梅等.加热处理对大豆扎清中胰蛋白酶抑制剂活性以及大豆孚L清蛋白体外消化率的影响.《食品工业科技》.2006,第27卷(第11期),全文. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101518295A (en) | 2009-09-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101518295B (en) | Method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons | |
| CN1275534C (en) | Corn peptide beverage and its processing method | |
| CN101143010A (en) | Special-purpose nutritive food for diabetes patient and its preparation method | |
| CN108812909A (en) | A kind of improvement partial eclipse or the diatery supplement nutriment of anorexia and preparation method thereof | |
| CN107183728A (en) | A kind of probiotics composite powder containing walnut peptide and preparation method thereof | |
| CN101461421A (en) | Method for processing egg milk fermented active lactic acid bacteria beverage | |
| CN101176485B (en) | Sour codonopsis lanceolata and preparation method thereof | |
| CN103329992A (en) | Preparation method of skim polypeptide cow milk | |
| CN107319569A (en) | A kind of high-purity silkworm chrysalis peptide functional foodstuff and preparation method thereof | |
| CN101341908A (en) | High-protein milk | |
| CN101697752A (en) | Sour milk added with casein phosphopeptide and preparation method thereof | |
| CN1943369A (en) | Collagen protein milk drink and its producing method | |
| CN101263861B (en) | Preparation animal protolysate | |
| CN104507334A (en) | Novel protein material | |
| CN103169092A (en) | L-asparaginic acid chelated calcium drinking water | |
| CN103798810A (en) | Method for preparing lactic acid bacteria powder containing multilayer microcapsule lactic acid bacteria | |
| CN110604295A (en) | Composite bone peptide and preparation method thereof | |
| CN106901235A (en) | A kind of instant egg-white powder of rich calcium and preparation method thereof | |
| CN101264317A (en) | Preparation of orally-administered protolysate | |
| CN103070396B (en) | Yak qula and calcium tablet health composition | |
| CN100362925C (en) | Method for preparing sour milk contg. micron additives for tonifying-Yin and eliminating-fatigue | |
| CN100333735C (en) | Fresh-bone high-calcium infusing prescription | |
| CN101264316B (en) | Preparation of medicinal protolysate | |
| CN1111094A (en) | Method for making high-calcium lactate milk | |
| CN1199573C (en) | Polypeptide fortified milk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20090902 Assignee: Zhejiang beiyinmeike Industry Co., Ltd. Assignor: Zhejiang University of Science and Technology Contract record no.: 2013330000125 Denomination of invention: Method for preparing powder rich in whey small peptides with molecular weight of 1000 daltons Granted publication date: 20130306 License type: Common License Record date: 20130506 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130306 Termination date: 20140225 |