CN101167758B - Method for preparing antiviral natural polymerization polysaccharide and oligosaccharide - Google Patents
Method for preparing antiviral natural polymerization polysaccharide and oligosaccharide Download PDFInfo
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Abstract
The invention discloses a process for preparing antiviral natural polymer polysaccharide and oligosaccharides, which settles the problems of the process for preparing the existing polysaccharide and the oligosaccharides, such as low purity, low yield, extensive investment capital and high cost of production. The invention takes edible fungi as raw material and prepares natural polymer polysaccharide and natural polymer oligosaccharides with high purity and excellent antiviral effect through new processing technology of biology bacterium cooperated with water logging extract, methylated alcohol, etc. Furthermore, the products also can be prepared to various oral capsule or oral liquid/powder electuary with different sizes. The process has low cost, high yield and expansive market outlook.
Description
Technical field
The present invention relates to the preparation method of a kind of polysaccharide and oligosaccharide.
Background technology
Find that through domestic and international medical research polysaccharide and oligosaccharide are in the human body immunity improving function, anti-virus aspect has extremely excellent performance, its health care is worth and has been subjected to extensive concern on market, along with technical development, the market demand of polysaccharide and oligosaccharide is also progressively improving, prior art for preparing polysaccharide and oligosaccharide generally all are the fruits with plant, stem, leaf, root, the mushrooms sporophore, pluck and fur are raw material, by water logging extraction and ethanol alcohol folding extracting method, through concentrating, ethanol alcohol is analysed the reaction back and is produced precipitate, and to drying after its collection, grinding and processing forms.Because in raw material, mushrooms raw material particularly, its active polysaccharide and oligosaccharide only have only trace to exist, and after processing, the amount that really finally can obtain polysaccharide and oligosaccharide is not high, and purity is also lower, makes that the infusion of financial resources amount is big, and production cost is extremely high.As being raw material with the mushrooms when in-depth processing is extracted, one ton of polysaccharide raw materials requirement of every processing amount is about 300 tons, the product recovery rate is about 0.3%, one ton of oligosaccharide raw materials requirement of every processing amount is about 3000 tons, recovery rate is about 0.03%, moreover, as alcohol analyse extract usefulness ethanol also up to 6 times of raw material, this Product industrialization is produced and the demand of market development thereby limited.
Summary of the invention
The present invention seeks to solve the problems of the technologies described above, provide a kind of purity height, production cost low, be easy to human body oral absorption, health-care effect strong, have the natural polymerization polysaccharide of excellent antiviral efficacy and a preparation method of natural polymerization oligosaccharide.
The preparation method of antiviral natural polymerization polysaccharide of the present invention, oligosaccharide, its step comprises:
(1) adopts water logging extraction, ethanol alcohol to analyse extracting method with antiviral drug-food plant raw material, obtain the drug-food plant polysaccharide through collecting precipitation thing, oven dry, pulverizing;
(2) above-mentioned drug-food plant polysaccharide directly added in the culture matrix mix thoroughly,, in culture matrix, insert to send in the culturing room behind each edible mushroom strain and cultivate then according to the edible fungi operational approach, with cultivate the white hypha body take out the back oven dry;
(3) the white hypha body that goes out with above-mentioned each edible mushroom breeding strain respectively is a raw material, adopts water logging extraction, ethanol alcohol to analyse extracting method, and collecting precipitation thing, oven dry, pulverizing obtain natural polymerization polysaccharide; To extract again alcohol extract behind the natural polymerization polysaccharide store leave standstill, post precipitation, extract supernatant and reclaim ethanol, with the surplus solution behind the recovery ethanol is raw material, concentrates, filters, adopts ethanol alcohol analysis method, and collecting precipitation thing, oven dry, pulverizing obtain the natural polymerization oligosaccharide.
In the described step (2) culture matrix and drug-food plant polysaccharide be preferably 1000: 40 by weight ratio～70, more be preferably 1000: 60, described culture matrix is frumentum, potato class or beans.
Alcohol extract in the described step (3) behind the extraction natural polymerization polysaccharide is stored and is left standstill, precipitates 60～100 days.
To be the natural polymerization polysaccharide that extracts of raw material with each edible mushroom strain in the described step (3) can be applicable to oral various dosage forms accordingly with making after the natural polymerization oligosaccharide mixes, as powder, tablet, oral solvent or capsule.
The present invention is a raw material with antiviral drug-food plant earlier, be prepared into drug-food plant polysaccharide powder, and the drug-food plant polysaccharide is added in the thick miscellaneous grain crops training substrate, be raw material with each edible mushroom strain again, adopt the biological bacteria novel technique, with the active substance that trace in medicinal or edible fungus and the plant exists, through absorbing, fission propagation, polymerization forms has multiple bioactive sugar chain.By substrate allotment, biosynthesis, increase the group effect of former drug-food plant, can balancedly satisfy human body requirements and the needs that are easy to absorb, utilize.By in culture matrix, adding the drug-food plant polysaccharide, make the component of interior polysaccharide of the mycelium of turning out and the active sugar chain of oligosaccharide increase, rise to more than 33% by original 14.7%, with it when extracting the raw material of natural polymerization polysaccharide and natural polymerization oligosaccharide, the recovery rate of product is largely increased, and then reduces with respect to the consumption of raw material and ethanol.
Raw material as the drug-food plant polysaccharide, present technique field personnel can choose the drug-food plant with antivirus action according to general knowledge and reference drug incompatibility, as in the Cortex Acanthopanacis Radicis of selecting to have antiviral efficacy, green tea, Bulbus Allii Cepae, the mao bamboo (Radix Crotalariae szemoensis), Fructus Lycii, Herb Gynostemmae Pentaphylli, RADIX SALVIAE MILTIORRHIZAE, Radix Isatidis, Herba Apii graveolentis, Fructus Cucurbitae moschatae, Fructus Momordicae charantiae, the Flos Chrysanthemi Indici etc. one or more, its each component proportioning does not limit, get final product with those skilled in the art's proportioning commonly used, as the prescription of preferably forming by Cortex Acanthopanacis Radicis, Herb Gynostemmae Pentaphylli, green tea, Bulbus Allii Cepae, bamboo sprout, Fructus Lycii.
As the edible bacterium of cultivating, optional enchashment has in the technology edible mushroom strain commonly used of preparation polysaccharide or oligosaccharide, as in Ganoderma, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, Cordyceps, JINZHENGU, Caulis Bambusae In Taeniam bacterium, Coriolous Dersicolor (Fr.) Quel, Brazilian mushroom, Chinese scholartree bolt bacterium, Poria, Marasmius androsaceus (L.ex Fr.) Fr., Agaricus blazei Murrill and the Grifola frondosa etc. one or more.
In culture matrix, add antiviral drug-food plant polysaccharide, make the former strain height that polyoses content is not more cultivated in its mycelium of cultivating, active sugar chain branch content height in the polysaccharide structures, and by the biological bacteria novel technique, the bacterium that reaches polysaccharide is planted compound, promptly the natural polymerization active polysaccharide natural polymerization of Ti Quing the multi-efficiency of mushroom polysaccharide and vegetable polysaccharides, as cultivating as edible bacterium with Ganoderma, in culture matrix, added drug-food plant polysaccharide with antiviral composition, thereby the polysaccharide component that active sugar chain extracts than prior art in the Ganoderma natural polymerization polysaccharide component that the inventive method makes has improved 19%, has had good antiviral activity.In like manner, the natural polymerization oligosaccharide of preparation also has above-mentioned feature.
Compare the drug regimen of the simple polysaccharide that occurs on the market, the inventor thinks also can allocate combination with the polysaccharide and the oligosaccharide that make, with the natural polymerization polysaccharide of each strain of obtaining via preparation method of the present invention respectively and natural polymerization oligosaccharide through suitable combination, can effectively prevent and treat at the various diseases that various different virus cause, have collaborative summation action between component, reach the maximization of treatment and health-care effect.
Can successively extract polysaccharide and oligosaccharide in the inventive method, technology is easy, and used device is few, and energy resource consumption is low, and, since the purity height, recovery rate height: purity of polysaccharide 〉=70%, recovery rate is 1.2%～2.5%, oligosaccharide purity 〉=75%, and recovery rate is 1 ‰～1.5 ‰.Make that relatively the ethanol consumption of raw material and conduct extraction solvent is few, production cost only is 1/8th of a prior art.In the technological operation, do not have any mildew-resistant of interpolation, agrochemical chemical substance, belong to natural component, meet the requirement of the world " pollution-free food ".It is edible for a long time that it is suitable for human body, have no side effect, can be according to by in culture matrix, adding the drug-food plant polysaccharide that makes by different antiviral drug-food plant prescriptions, and choose natural polymerization polysaccharide and the natural polymerization oligosaccharide that suitable edible mushroom strain preparation goes out each mushrooms strain at different viral diseases; Further, can as required the powder of natural polymerization polysaccharide and natural polymerization oligosaccharide be allocated again, antibiotic as an alternative health care dietary product, be easy to oral, be easy to absorb, curative effect is good, has vast market prospect.
Embodiment 1
One, the preparation of raw material:
1. strain: the test tube kind after Ganoderma, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, the Cordyceps rejuvenation.
2. antiviral drug-food plant raw material prescription: Cortex Acanthopanacis Radicis 1000 gram, Herb Gynostemmae Pentaphylli 800 grams, green tea 400 grams, Bulbus Allii Cepae 1000 grams, bamboo sprout 800 grams, Fructus Lycii 1000 grams, total amount is 5000 grams, requires wild that excavate, the free from insect pests of rich selenium not have the raw material that goes mouldy.
3. handle the early stage of culture matrix:
(1) raw material: corn 1000 gram, requiring the free from insect pests of rich selenium area farm nanure planting not have the corn that goes mouldy is raw material.
(2) preparation method: with standby corn pour into soak 24-36 hour in 1% the lime solution (supernatant) after, to kill mycete and insect in the corn, pour out soak, with corn flushing repeatedly in clear water, make acid-base value PH reach 6-7, the dried excessive moisture of weeping then, dry up surface moisture after, standby.
4. extraction preparing solvent:
(1) raw material: 65 degree grain wine of 95 degree edible ethanol, corn or corn brews of corn or corn brew.
(2) preparation: the grain wine of getting brews such as corn or corn brew edible ethanol and corn mixes by weight at 4: 1, comprehensive back wine degree is 88～90 degree, adds active carbon for liquor and stirs, filter, leave standstill 48 hours after, extract supernatant, it is standby to make the extraction solvent.
Two, preparation method:
1. the manufacture method extracted of drug-food plant polysaccharide:
Antiviral drug-food plant raw material is respectively charged in the rustless steel container by above-mentioned weight, the filtration tap water that adds the treble amount, heat, make water temperature in 85 ℃, kept 85～100 minutes, pour out extracting solution, the filtration tap water that adds isodose more still keeps water temperature at 85 ℃, keeps 60 minutes, pour out extracting solution for the second time, waste; Extracted twice liquid is merged, after the filtration, it is concentrated to heat, and is concentrated at about 1/3 o'clock, from fire, container is put into the cold water cooling, after 4～6 hours, extract supernatant, the extraction solvent that in supernatant, adds 60 kilograms, stir, produced smalls shape precipitate in about 1～2 minute, leave standstill 4 hours after, extract supernatant, reuse extracts the solvent washing precipitate 2～3 times, collects precipitate, the dried solution of weeping, in 85 ℃ air dry oven, dry, pulverize, cross 80 mesh sieves, make drug-food plant polysaccharide (purity is 40～50%, and recovery rate is 1%-1.5%), standby.
2. bacteriumization cultivation:
The white sugar that above-mentioned standby corn is added 10 grams, the drug-food plant polysaccharide powder of the edible soda ash of 1 gram and 60 grams, stir, stack after 10 minutes, allow it fully dissolve, after the absorption, be respectively charged in the wide-mouth vial of 400-650 gram, clean the outer foreign material of bottle, seal with Polypropylence Sheet and paper, keep sterilizing in 8 hours after in pressure cooker (1.25kg pressure/40 minute) and normal-pressure sterilization pot, going up gas, after the cooling, put into the aseptic inoculation case, press the edible fungi operational approach, insert Ganoderma respectively, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, the test tube strains of Cordyceps is (after mycelia covers with full bottle, can continue switching enlarges), in 20-28 ℃ of constant temperature, cultivated about 30 days, after the mycelium for the treatment of white covers with whole cultivation bases, draw out respectively, oven dry, standby.During cultivation, yellow, black, green etc. should in time remove when variegated if occur in the bottle.
3. in-depth is extracted and is made:
(1) extraction of natural polymerization polysaccharide:
Pour into the standby mycelium raw material that various edible bacteriums are cultivated in the rustless steel container respectively, after adding the filtration tap water of 3 times of amounts of mycelium raw material, heat, water temperature was kept in 85 ℃ 85～100 minutes, pours out extracting solution for the first time, add the filtration tap water of 3 times of amounts again after, make water temperature in 85 degree, kept 85～100 minutes, and poured out extracting solution for the second time, waste.Extracted twice liquid is merged after-filtration, heat and be concentrated into three/for the moment, from fire, container is put into after cold water cooling leaves standstill 24 hours, extract supernatant, in supernatant, pour the extraction solvent of 4 times of amounts into, stir, about 1～2 minute Powdered polysaccharide precipitation thing of generation yellow-white, after leaving standstill 4 hours, pour out alcohol extract, collect precipitate, reuse extracts solvent washing 2～3 times, weep behind the dried solvent, in 85 ℃ air blast dehydrator, dry respectively, pulverize, obtain Ganoderma respectively after crossing 80 mesh sieves, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, the Powdered natural polymerization active polysaccharide of Cordyceps, purity of polysaccharide 〉=70%, recovery rate is 1.2%-2.5%.Alcohol extract behind the extraction polysaccharide is stored respectively in the ceramic wine jar by different strain, leaves standstill 60-100 days, and is standby.
(2) extraction of natural polymerization oligosaccharide:
Above-mentioned each standby edible mushroom strain alcohol extract is extracted supernatant, reclaim ethanol at wine in recycling can respectively, yield reaches at 3/5ths o'clock, stop to reclaim, heat when being concentrated into sticking wooden dipper (promptly to surplus solution total amount about 1/3rd) reclaiming surplus solution behind the ethanol, from fire, in cold water, cool off, after leaving standstill 24 hours, extract supernatant, pour the extraction solvent of 6 times of amounts into, stir, brown smalls reaction precipitation thing appears in the solution in the time of about 2～3 minutes, after leaving standstill 12 hours, pour out alcohol extract, after reuse extracts solvent washing 2～3 times, the tacky precipitate of dark brown is collected, pack in the stainless steel disc, in 85 ℃ air dry oven, dry respectively, pulverize, cross 80 mesh sieves, obtain Ganoderma respectively, Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, the Powdered natural polymerization oligosaccharide of Cordyceps etc., oligosaccharide purity 〉=75%, recovery rate are 1 ‰-1.5 ‰.
Three, diet therapy formulation proportioning (is unit of account with 1000 gram products):
Be used to prevent and treat various malignant tumor and the allotment of syndromic dietary product thereof that bacterial virus causes:
(1) prescription: Ganoderma natural polymerization polysaccharide 300 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization polysaccharide 200 grams, Cordyceps natural polymerization polysaccharide 100 grams, Lentinus Edodes natural polymerization polysaccharide 100 grams, Ganoderma natural polymerization oligosaccharide 100 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization oligosaccharide 50 grams, Cordyceps natural polymerization oligosaccharide 100 grams, Lentinus Edodes natural polymerization oligosaccharide 50 grams.
(2) processing: be processed into capsule, every 0.6 gram.
(3) eating method: oral, every day three times, each three to five, 30 days is a course of treatment.
Embodiment 2
One, the preparation of raw material:
1. strain: the test tube kind after Ganoderma, Hericium erinaceus (Bull. Ex Fr.) Pers., Cordyceps, the Brazilian mushroom rejuvenation.
2. antiviral drug-food plant raw material prescription: Herb Gynostemmae Pentaphylli 2000 restrains, green tea 1500 restrains,
Bulbus Allii Cepae 800 grams, Radix Isatidis 700 grams, total amount is 5000 grams.
3. culture matrix: with embodiment 1.
4. extraction solvent: 90 degree grain wine of corn or corn brew.
Two, preparation method: wherein, the drug-food plant polysaccharide powder that adds in corn culture medium matter is 70 grams, and all the other methods are with embodiment 1.
Three, diet therapy formulation proportioning (is unit of account with 1000 gram products):
1, the dietary product allotment of diseases such as the acquired immune deficiency syndrome (AIDS) that causes of control bacterial virus, SARS, bird flu:
(1) prescription: Ganoderma natural polymerization polysaccharide 300 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization polysaccharide 150 grams, Cordyceps natural polymerization polysaccharide 150 grams, Brazilian mushroom natural polymerization polysaccharide 100 grams, Ganoderma natural polymerization oligosaccharide 100 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization oligosaccharide 50 grams, Cordyceps natural polymerization oligosaccharide 100 grams, low natural polymerization oligosaccharide 50 grams of Brazilian mushroom.
(2) processing: with above raw material stirring evenly after, be processed into tabletting, every 0.6 gram.
(3) eating method: oral, every day three times, each three to five, 30 days is a course of treatment.
Embodiment 3
One, the preparation of raw material:
1. strain: the test tube kind after Ganoderma, Cordyceps, Chinese scholartree bolt bacterium, Agaricus blazei Murrill, the Hericium erinaceus (Bull. Ex Fr.) Pers. rejuvenation.
2. antiviral drug-food plant raw material prescription: Bulbus Allii 1000 grams, Radix Isatidis 1500 grams, green tea 1500 grams, bamboo sprout 500 grams, Flos Chrysanthemi Indici 500 grams, total amount is 5000 grams.
3. culture matrix: with embodiment 1.
4. extraction solvent: with embodiment 1.
Two, preparation method: wherein, the drug-food plant polysaccharide powder that adds in corn culture medium matter is 40 grams, and all the other are with embodiment 1.
Three, diet therapy formulation proportioning (is unit of account with 1000 gram products):
Disease dietary product allotments such as the various hepatitis that the control bacterial virus causes, fatty liver, liver cirrhosis:
(1) prescription: Ganoderma natural polymerization polysaccharide 250 grams, Cordyceps natural polymerization polysaccharide 200 grams, Chinese scholartree bolt bacterium natural polymerization polysaccharide 150 grams, Agaricus blazei Murrill natural polymerization polysaccharide 100 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization polysaccharide 100 grams, low natural polymerization oligosaccharide 80 grams of Ganoderma, Cordyceps natural polymerization oligosaccharide 70 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization oligosaccharide 50 grams.
(2) processing: with above raw material stirring evenly after, be processed into powder, every bag 0.6 gram.
(3) eating method: oral, every day three times, each four to six bags, 30 days is a course of treatment.
Embodiment 4
One, the preparation of raw material:
1. strain: the test tube kind after Hericium erinaceus (Bull. Ex Fr.) Pers., Lentinus Edodes, Coriolous Dersicolor (Fr.) Quel, Cordyceps, Caulis Bambusae In Taeniam bacterium, the Grifola frondosa rejuvenation.
2. antiviral drug-food plant raw material prescription: green tea 1500 grams, Bulbus Allii 1000 grams, Bulbus Allii Cepae 1000 grams, Cortex Acanthopanacis Radicis 800 grams, RADIX SALVIAE MILTIORRHIZAE 700 grams, total amount is 5000 grams.
3. culture matrix: with embodiment 1.
4. extraction solvent: with embodiment 1.
Two, preparation method: with embodiment 1.
Three, diet therapy formulation proportioning (is unit of account with 1000 gram products):
Prevent and treat the dietary product allotment of various bacterial disease toxicity myocarditiss, viral pneumonia, viral enteritis disease:
(1) prescription: Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization polysaccharide 300 grams, Lentinus Edodes natural polymerization polysaccharide 150 grams, Coriolous Dersicolor (Fr.) Quel natural polymerization polysaccharide 150 grams, Cordyceps natural polymerization polysaccharide 100 grams, Caulis Bambusae In Taeniam bacterium natural polymerization polysaccharide 100 grams, Grifola frondosa polymerization polysaccharide 50 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization oligosaccharide 50 grams, Coriolous Dersicolor (Fr.) Quel natural polymerization oligosaccharide 30 grams, Lentinus Edodes natural polymerization oligosaccharide 30 grams, Cordyceps natural polymerization oligosaccharide 20 grams, Caulis Bambusae In Taeniam bacterium natural polymerization oligosaccharide 20 grams.
(2) processing: with above raw material stirring evenly after, be processed into capsule or tabletting, every (sheet) 0.6 gram.
(3) eating method: oral, every day secondary, each four to six, 30 days is a course of treatment.
Embodiment 5
One, make the preparation of raw material:
1. strain: the test tube kind after Ganoderma, Caulis Bambusae In Taeniam bacterium, Cordyceps, Poria, the Marasmius androsaceus (L.ex Fr.) Fr. rejuvenation.
2. antiviral drug-food plant raw material prescription: green tea 1500 grams, Herb Gynostemmae Pentaphylli 800 grams, Fructus Cucurbitae moschatae 800 grams, Cortex Acanthopanacis Radicis 500 grams, Fructus Momordicae charantiae 500 grams, RADIX SALVIAE MILTIORRHIZAE 500 grams, Fructus Lycii 400 grams, total amount is 5000 grams.
3. culture matrix: Semen Tritici aestivi 1000 grams, preparation method is with embodiment 1.
4. extraction solvent: with embodiment 2.
Two, preparation method: with embodiment 1.
Three, diet therapy formulation proportioning (is unit of account with 1000 gram products):
Prevent and treat cardiovascular disease, diabetes, obesity and the allotment of symptomes complice dietary product thereof:
(1) prescription: Ganoderma natural polymerization polysaccharide 300 grams, Caulis Bambusae In Taeniam bacterium natural polymerization polysaccharide 200 grams, Cordyceps natural polymerization polysaccharide 150 grams, Poria natural polymerization polysaccharide 100 grams, Marasmius androsaceus (L.ex Fr.) Fr. natural polymerization polysaccharide 100 grams, Ganoderma natural polymerization oligosaccharide 80 grams, Caulis Bambusae In Taeniam bacterium natural polymerization oligosaccharide 30 grams, Marasmius androsaceus (L.ex Fr.) Fr. natural polymerization oligosaccharide 20 grams, Cordyceps natural polymerization oligosaccharide 20 grams.
(2) processing: with above raw material stirring evenly after, be processed into capsule or tabletting, every (sheet) 0.6 gram.
(3) eating method: oral, every day secondary, each four to six, took in 8 o'clock sooner or later, 30 days is a course of treatment.
Embodiment 6
One, the preparation of raw material:
1. strain: the test tube kind after Hericium erinaceus (Bull. Ex Fr.) Pers., Ganoderma, Cordyceps, JINZHENGU, the Poria rejuvenation.
2. antiviral drug-food plant raw material prescription: green tea 2000 grams, bamboo sprout 1000 grams, Fructus Lycii 1000 grams, the Radix Aucklandiae 200 grams, Rhizoma Dioscoreae 800 grams, total amount is 5000 grams.
3. culture matrix: Semen Tritici aestivi 1000 grams, preparation method is with embodiment 1.
4. extraction solvent: with embodiment 1.
Two, preparation method: with embodiment 1.
Three, diet therapy formulation proportioning (is unit of account with 1000 gram products):
The digestive tract tumor that control pylori virus causes, old gastric ulcer, gastritis and the allotment of syndromic dietary product thereof:
(1) prescription: Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization polysaccharide 400 grams, Ganoderma natural polymerization polysaccharide 150 grams, Cordyceps natural polymerization polysaccharide 100 grams, JINZHENGU natural polymerization polysaccharide 100 grams, Poria natural polymerization polysaccharide 100 grams, Hericium erinaceus (Bull. Ex Fr.) Pers. natural polymerization oligosaccharide 100 grams, Ganoderma natural polymerization oligosaccharide 50 grams.
(2) processing: with above raw material stirring evenly after, be processed into capsule or tabletting, every (sheet) 0.6 gram.
(3) eating method: oral, every day three times, each three to four, 30 days is a course of treatment.
Claims (7)
1. the preparation method of a natural polymerization polysaccharide, oligosaccharide is characterized in that its step comprises:
(1) is that raw material adopts water logging extraction, ethanol alcohol to analyse extracting method with antiviral drug-food plant, obtains the drug-food plant polysaccharide through collecting precipitation thing, oven dry, pulverizing;
(2) above-mentioned drug-food plant polysaccharide directly added in the culture matrix mix thoroughly,, in culture matrix, insert to send in the culturing room behind each edible mushroom strain and cultivate then according to the edible fungi operational approach, with cultivate the white hypha body take out the back oven dry;
(3) the white hypha body that goes out with above-mentioned each edible mushroom breeding strain respectively is a raw material, adopts water logging extraction, ethanol alcohol to analyse extracting method, and collecting precipitation thing, oven dry, pulverizing obtain natural polymerization polysaccharide; To extract again alcohol extract behind the natural polymerization polysaccharide store leave standstill, post precipitation, extract supernatant and reclaim ethanol, with the surplus solution behind the recovery ethanol is raw material, concentrates, filters, adopts ethanol alcohol analysis method, and collecting precipitation thing, oven dry, pulverizing obtain the natural polymerization oligosaccharide.
2. the preparation method of natural polymerization polysaccharide as claimed in claim 1, oligosaccharide is characterized in that, described antiviral drug-food plant is made up of Cortex Acanthopanacis Radicis, Herb Gynostemmae Pentaphylli, green tea, Bulbus Allii Cepae, bamboo sprout, Fructus Lycii.
3. the preparation method of natural polymerization polysaccharide as claimed in claim 1, oligosaccharide is characterized in that, culture matrix and drug-food plant polysaccharide are 1000: 40～70 by weight ratio in the described step (2).
4. the preparation method of natural polymerization polysaccharide as claimed in claim 1, oligosaccharide is characterized in that, culture matrix and drug-food plant polysaccharide are 1000: 60 by weight ratio in the described step (2).
5. the preparation method of natural polymerization polysaccharide as claimed in claim 1, oligosaccharide, described culture matrix are frumentum, potato class or beans.
6. the preparation method of natural polymerization polysaccharide as claimed in claim 1, oligosaccharide is characterized in that, the alcohol extract in the described step (3) behind the extraction natural polymerization polysaccharide is stored and left standstill, precipitates 60～100 days.
7. as the preparation method of the described natural polymerization polysaccharide of each claim of claim 1～6, oligosaccharide, it is characterized in that will be the natural polymerization polysaccharide that successively extracts of raw material with each edible mushroom strain in the described step (3) can be applicable to oral various dosage forms with making after the natural polymerization oligosaccharide mixes.
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| CN109771433A (en) * | 2019-03-05 | 2019-05-21 | 严正华 | The low molecule oligosaccharide composition for preventing and treating hepatitis B, liver cancer |
| CN115737494B (en) * | 2022-10-19 | 2024-05-14 | 菲朗生物科技(湖北)有限公司 | Antioxidant moisturizing skin care product and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1116906A (en) * | 1995-04-26 | 1996-02-21 | 严正华 | Health food mushroom essence and its preparing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1116906A (en) * | 1995-04-26 | 1996-02-21 | 严正华 | Health food mushroom essence and its preparing method |
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