CN101138574A - Wine-fried angelica decocting pieces, preparation method thereof and application thereof - Google Patents
Wine-fried angelica decocting pieces, preparation method thereof and application thereof Download PDFInfo
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- CN101138574A CN101138574A CNA200710066149XA CN200710066149A CN101138574A CN 101138574 A CN101138574 A CN 101138574A CN A200710066149X A CNA200710066149X A CN A200710066149XA CN 200710066149 A CN200710066149 A CN 200710066149A CN 101138574 A CN101138574 A CN 101138574A
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Abstract
The invention provides a processing method of a liquor angelica slice for promoting coronary circulation. The angelica without wiping off root and skin is undertaken the procedures of purifying, rinsing, soaking, cutting into thin slices, drying or baking. A certain amount of rice wine is added for mixing, oppressing, stir-frying and cooling. Compared with raw angelica, the liquor angelica slice processed in the method has obvious effect of inhibiting uterine smooth muscle contraction and anti-platelet aggregation and anti-pain effect. The invention can be used as a recipe for Chinese medicine, or finished medicine and natural medicine, as well as an ingredient for health products such as health food and cosmetics of special purpose. The processing method is simple and easy for quality control and industrial production. The application of the method provides a standardized procedure for the processing of traditional Chinese angelica.
Description
Technical field:
The present invention relates to a kind of raw material of Chinese medicine medicated wine fried angelica decocting pieces, its preparation method and its are in urging property of preparation medicine or sexual function improving medicine, in the preparation medicament for resisting platelet aggregation and in the application of preparation in the analgesic.
Technical background: Radix Angelicae Sinensis is the famous Chinese medicine material of China, is the root of umbelliferae angelica (Angelica sinensis L.).The use of Radix Angelicae Sinensis is with a long history.See " elegant " the earliest, " Pi " and " beg on the mountain " in the book are Radix Angelicae Sinensis.Shennong's Herbal is classified middle product as, claims its " housewife people is leaked down, sterilization, all malignant boil infectionss, incised wound is boiled drink ".Sweet in the mouth, suffering, warm in nature.Return liver, the heart, spleen channel.Effects such as having enriches blood invigorates blood circulation, menstruction regulating and pain relieving, loosening bowel to relieve constipation.Be used for blood deficiency and yellow complexion, dizzy cardiopalmus, menoxenia, amenorrhea dysmenorrhoea, asthenia cold abdominalgia, dryness of the intestine constipation, rheumatic arthralgia, injury from falling down, ulcer sores etc.In tcm prescription, use in a large number, have saying of " ten side's rules for doing division with a one-digit divisor on the abacus ", to reach hundreds of when the Chinese patent medicine that is classified as raw material.
The concocting method of Radix Angelicae Sinensis is recorded in Southern Qi Dynasty's " Liu Juanzi Guiyi Fang, Liu Juanzi " the earliest.The concocting method of summary Radix Angelicae Sinensis such as Wang Lichun has 21 kinds, and now commonly used have only 7 kinds of processed with wine, parch, charcoal processings etc., and main rising changes the property of medicine, enlarges the effect of range of application.Yu Shaojun etc. relatively discover, Cu in the processed with wine decoction pieces, and Ni increases, and Pb reduces; Fe in the soil system decoction pieces, Ni, Cu, Mn, Zn increases, and Pb reduces; Parch to black decoction pieces Ca, Ni increases, and Pb reduces; But Ni in fried liquid, Pb, Ca, the rule of Mg etc. is opposite.Zhang Jian etc. have studied the volatile oil content (official method) of Radix Angelicae Sinensis processed product, find that Radix Angelicae Sinensis contains 0.39% (ml/100g) orange-yellow oil, Radix Angelicae Sinensis (processed with wine) contains 0.21% (ml/100g) orange-yellow oil, Radix Angelicae Gigatis contains 0.06% (ml/100g) brownish red oil, and Radix Angelicae Sinensis carbon contains 0.02% (ml/100g) yellow oil.Think that the grease chamber is destroyed by high temperature and causes variation.Proposition ferulaic acid content Radix Angelicae Sinensis such as Gao Fengxi＞fry＞wine＞soil＞charcoal.Yu Shaojun etc. discover that relatively processed with wine decoction pieces, soil system decoction pieces, parch to black decoction pieces tannin increase, and ferulic acid, alcohol extract reduce; Water extraction is a height with the processed with wine decoction pieces.Rein in research comparisons such as wind and cloud, think that water-soluble sugar, reducing sugar and polysaccharide are all the highest with processed with wine decoction pieces content, soil system decoction pieces decreases, respectively at 40-55%, and 40-50% and 4-6%.Wu Hui equality
[8]The lipoid peroxidization resistant of the Radix Angelicae Sinensis of more different concocting methods is found active size according to Radix Angelicae Sinensis carbon＞burnt Radix Angelicae Sinensis＞Radix Angelicae Sinensis (processed with wine)＞Radix Angelicae Sinensis＞Radix Angelicae Gigatis rule, and is relevant with what of composition such as tannin in the Radix Angelicae Sinensis.In addition, operation such as also useful sulfur fumigation and removal epidermis is attractive in appearance to increase it in the conventional process of Radix Angelicae Sinensis.
A large amount of research reports show that the chemical constituent of Radix Angelicae Sinensis is relevant with concocting method with physiologically active.Have different chemical characteristics and physiological action with a kind of decoction pieces, usually being that processing technique is different produces.Therefore, the concocting method of angelica sinensis standard is very necessary for the quality that guarantees decoction pieces and the concordance of effect.
Summary of the invention:
The present invention aims to provide a kind of Wine-fried angelica decocting pieces, its concocting method.This concocting method is simple, is easy to scale, standardization production, can effectively control production process and product quality.Wine-fried angelica decocting pieces by the present invention's production, have the contraction of significant inhibition uterine smooth muscle, antiplatelet aggregation and analgesic activity, can be used as the tcm and herbal slice of blood circulation promoting and blood stasis dispelling, the prescription that is used for Chinese medicine, also can be used as Chinese patent medicine and natural drug, and the raw material of health-oriented products such as functional health care food and caused by cosmetics for special use.The process of preparing Chinese medicine that is applied as the Chinese medicine angelica sinensis of the present invention provides normalized method.
For realizing above-mentioned purpose of the present invention, the invention provides following technical scheme:
A kind of Wine-fried angelica decocting pieces, wherein the content of ferulic acid and ligustilide is respectively 0.0889 μ g and 0.3970 μ g.
A kind of Wine-fried angelica decocting pieces, to make as follows when being classified as raw material:
The Radix Angelicae Sinensis of root bark, remove impurity, rinsing will do not removed, soak into, cutting is the thin slice of thickness 1.7-2.7mm, and baking temperature is no more than and dries under 45 ℃ or dry, add the yellow wine of 10% proportional quantities, mix thoroughly, vexed, inhaled profit 5-7 hour, and exhausted to yellow wine, till the decoction pieces tool aroma, put in the pot, be no more than 50 ℃ with temperature, time 50-60 minute slow fire fries decoction pieces to buff, the tool aroma is taken out, and is lower than 19% to the decoction pieces water content and puts cold getting final product.
The preparation method of above-mentioned Wine-fried angelica decocting pieces will not removed the Radix Angelicae Sinensis of root bark, remove impurity, rinsing, soak into, cutting is the thin slice of thickness 1.7-2.7mm, and baking temperature is no more than and dries under 45 ℃ or dry, add the yellow wine of 10% proportional quantities, mix thoroughly, vexed, inhaled profit 5-7 hour, and exhausted to yellow wine, till the decoction pieces tool aroma, put in the pot, be no more than 50 ℃ with temperature, time 50-60 minute slow fire fries decoction pieces to buff, the tool aroma is taken out, and is lower than 19% to the decoction pieces water content and puts cold getting final product.
A kind of Wine-fried angelica decocting pieces granule is that raw material makes as follows with the Radix Angelicae Sinensis (processed with wine) sheet:
Decoct with water, adding drinking-water for the first time and be 10 times of medical material amounts, is 8 times for the second time, is 6 times for the second time, before for the first time opening steam, soaked into 30 minutes earlier, pick up counting, be respectively 2 hours, 1.5 hours and 1.5 hours for 3 times with boiling, merge extractive liquid,, concentrate below 80 ℃,, get extractum to relative density 1.37-1.40 (80 ℃); Get lactose and mannitol,, get adjuvant according to mixing in 1: 1; With extractum: adjuvant 1: the 5-6 mixed is granulated to appropriateness, crosses 20 mesh sieves, and 50 ℃ of dryings are crossed 20 mesh sieve granulate, and 50 ℃ of dryings 30 minutes promptly get the Wine-fried angelica decocting pieces granule of moisture 6%.
In addition, the present invention also provides above-mentioned angelica sinensis or angelica sinensis granule to return application in the medicine of section's mass formed by blood stasis in preparation treatment or prevention; Application in the preparation medicament for resisting platelet aggregation and the application in the preparation analgesic.
The wine of the tool function of promoting blood circulation to disperse blood clots that the above-mentioned processing procedure of process obtains is processed Radix Angelicae Sinensis and is concocted decoction pieces, the physiologically active that suppresses uterine smooth muscle contraction, analgesia and antiplatelet aggregation three aspects is all significantly greater than the Radix Angelicae Sinensis sheet, the safety and the effectiveness of Radix Angelicae Sinensis clinical practice have been guaranteed to concoct, and concocting process is simple, is easy to quality control and suitability for industrialized production.
The Radix Angelicae Sinensis that is used in the technique scheme concoct is not removed root bark.In view of ferulic acid in the root bark and ligustilide content all are higher than the peeling Radix Angelicae Sinensis and head is returned in peeling.Histochemical studies shows that volatile ingredients such as ligustilide are stored in You Shijia more, and ferulic acid etc. also mainly are distributed in phloem, and therefore, described Radix Angelicae Sinensis is not removed root bark.
It is the thin slice of thickness 1.7-2.7mm that the Radix Angelicae Sinensis that is used to concoct in the technique scheme is answered cutting.The variation of moisture and volatile ingredient when the thickness of section not only influences drying, the stripping of effective ingredient when also influence is extracted, decoction pieces is crossed the thin absorption once more that also may produce effective ingredient etc.
The temperature of dry Chinese angelica slicing is advisable to be no more than 50 ℃ in the technique scheme.Though in 40 ℃ of exsiccant Chinese angelica slicings, the content of ferulic acid and ligustilide is than 35 ℃ and 50 ℃ of exsiccant height.Owing to also contain abundant volatile material and thermally labile component in the Radix Angelicae Sinensis, be the effective ingredient of Radix Angelicae Sinensis, temperature is too high in the dry run, very easily destroys.Therefore, the baking temperature of described Radix Angelicae Sinensis Tabellae is advisable to be no more than 50 ℃.
The proportional quantities that adds yellow wine in the technique scheme is 10%.An amount of with wine, can promote the stripping of composition, but it is too many to add capacity for liquor, also may make some ingredients from lossing.Experimental result shows, the content of ferulic acid and ligustilide with the proportional quantities that adds yellow wine be 10% o'clock the highest.
After adding yellow wine in the technique scheme, need mix thoroughly, sealing was soaked into 5-7 hour, to vexed, and decoction pieces tool aroma.
The temperature that is used for parch in the technique scheme is advisable to be no more than 50 ℃.Experimental result shows, parch such as smokes at the active constituent content height of additive method, and production process is easy to control.Parch has exsiccant effect concurrently, and temperature is too low, needs the long time, the obvious zoom of decoction pieces that moderate heat and intense fire are fried, and the extracting solution color is orange.Experimental result shows that when the parch temperature was no more than 50 ℃, ferulaic acid content was the highest.The content of ligustilide then increases with temperature and reduces.Usually, parch is in the time of 20 minutes, and decoction pieces has been pale brown color, and matter is pliable and tough, the tool wine aroma.Parch is in the time of 30 minutes, and the decoction pieces color is darker, and matter is crisp firmly.In the actual plant produced, it is big to throw the sample amount, parch time and stir frequency, stir number of times etc., all prepared slice quality is had considerable influence.So do not stipulate the concrete parch time that with the pale brown color of decoction pieces, matter is pliable and tough, water content is no more than 10%, and the tool wine aroma is degree.Angelica sinensis after parch, put cold, the packing.
Have the contraction of the uterine smooth muscle of inhibition, antiplatelet aggregation and analgesic activity with the Wine-fried angelica decocting pieces for preparing in the technique scheme.The tcm and herbal slice that can be used as blood circulation promoting and blood stasis dispelling is used for the prescription of Chinese medicine, also can be used as Chinese patent medicine and natural drug, and the raw material of health-oriented products such as functional health care food and caused by cosmetics for special use.
Being used for applying high voltage liquid chromatography (HPLC) analytic process in the technique scheme, is index with the content of main component ferulic acid and ligustilide in the Radix Angelicae Sinensis, and the process of preparing Chinese medicine production technology of Radix Angelicae Sinensis is investigated, and the standardization Radix Angelicae Sinensis is concocted production routine.
The Wine-fried angelica decocting pieces granule that relates among the present invention and the preparation method of Radix Angelicae Sinensis granule adopt:
Radix Angelicae Sinensis granule: raw material: Radix Angelicae Sinensis decoction pieces.Concocting process: get the Radix Angelicae Sinensis sheet, decoct with water.For the first time adding drinking water is 10 times of medical material amounts, is 8 times for the second time, is 6 times for the second time.Before for the first time opening steam, soaked into 30 minutes earlier.Pick up counting with boiling, be respectively 2 hours, 1.5 hours and 1.5 hours for 3 times.Merge extractive liquid, concentrates below 80 ℃, to relative density 1.37-1.40 (80 ℃), gets extractum.Get lactose and mannitol,, get adjuvant according to mixing in 1: 1.With extractum: adjuvant 1: the 5-6 mixed is granulated to appropriateness, crosses 20 mesh sieves.50 ℃ of dry appropriateness (to dry), granulate (crossing 20 mesh sieves).50 ℃ of dryings 30 minutes, (moisture 6%), the Radix Angelicae Sinensis granule.
Wine-fried angelica decocting pieces granule: raw material: Radix Angelicae Sinensis (processed with wine) sheet.Concocting process: get the Radix Angelicae Sinensis (processed with wine) sheet, decoct with water.For the first time adding drinking water is 10 times of medical material amounts, is 8 times for the second time, is 6 times for the second time.Before for the first time opening steam, soaked into 30 minutes earlier.Pick up counting with boiling, be respectively 2 hours, 1.5 hours and 1.5 hours for 3 times.Merge extractive liquid, concentrates below 80 ℃, to relative density 1.37-1.40 (80 ℃), gets extractum.Get lactose and mannitol,, get adjuvant according to mixing in 1: 1.With extractum: adjuvant 1: the 5-6 mixed is granulated to appropriateness, crosses 20 mesh sieves.50 ℃ of dry appropriateness (to dry), granulate (crossing 20 mesh sieves).50 ℃ of dryings 30 minutes (moisture 6%), the Wine-fried angelica decocting pieces granule.
Description of drawings:
Fig. 1 is the canonical plotting of ferulic acid in the Wine-fried angelica decocting pieces of the present invention;
Fig. 2 is the quantitative analysis ideograph of the HPLC of ferulic acid in the Wine-fried angelica decocting pieces of the present invention;
Fig. 3 is the canonical plotting of ligustilide in the Wine-fried angelica decocting pieces;
Fig. 4 is the quantitative analysis ideograph of the HPLC of ligustilide in the Wine-fried angelica decocting pieces of the present invention;
Fig. 5 processs the HPLC quantitative analysis ideograph of Radix Angelicae Sinensis for wine of the present invention.
The specific embodiment:
Below in conjunction with accompanying drawing, further specify essentiality content of the present invention with embodiments of the invention, but do not limit the present invention with this.
Embodiment 1:
Concoct the angelica sinensis of tool function of promoting blood circulation to disperse blood clots:
(1) remove impurity: get the Radix Angelicae Sinensis of not removing root bark, adopt the method for picking to adopt the method for picking to remove weeds and silt with sieving.
(2) rinsing: will put into clear water (meeting the drinking water rank) through the Radix Angelicae Sinensis that remove impurity is handled and carry out rinsing, and remove the dust be stained with on Radix Angelicae Sinensis medical material surface etc.To avoid the operating time long in the rinsing, cause hindering water.
(3) soak into: the Radix Angelicae Sinensis medical material is tiled on the medicine platform, shows to add the drop cloth sheet.Observe the situation that moisture infiltrates medical material in the put procedure, suitable spray water after moisture runs through (method of pinching with bending method or hands is judged) with Radix Angelicae Sinensis body, the afterbody of Radix Angelicae Sinensis medical material, will be returned head and return body, tail to cut, return body, tail to enter the cutting operation, return head to proceed to soak into.This moment, the frequency and the degree of water spray can suitably strengthen, and were run through (judging with bending method or the hands method of pinching) until Radix Angelicae Sinensis.(soaked into about 12 hours, the extremely saturating heart is degree.)
(4) cutting: will in time carry out cutting through the Radix Angelicae Sinensis medical material that soaks into, specification is a thin slice, and promptly decoction pieces thickness is about 1.7～2.7mm.
(5) drying: the Radix Angelicae Sinensis of section is dried or dry (baking temperature is no more than 45 ℃), be degree (adopting hands to pinch method or bending method judgement) to moisture 8-12%.Sieve dedust bits.
(6) get above-mentioned Radix Angelicae Sinensis decoction pieces 500g, add the 50g yellow wine, mix thoroughly, vexed (inhaled profit about 5-7 hour, and exhausted to yellow wine, till the decoction pieces tool aroma), put in the pot, with slow fire with the decoction pieces parching to brown (temperature is no more than 50 ℃, time 50-60 minute, to the decoction pieces buff, the tool aroma), take out, put cold (in the moisture 10%), get the angelica sinensis of tool function of promoting blood circulation to disperse blood clots.
With test example of the present invention essentiality content of the present invention and superiority are described below.
Test example 1:
The quality standard of Wine-fried angelica decocting pieces: the high pressure liquid chromatography of ferulic acid and ligustilide (HPLC) quantitative analysis is carried out by the following method in the Wine-fried angelica decocting pieces.
(1) high pressure liquid chromatography of ferulic acid (HPLC) quantitative analysis:
Instrument and reagent: the HPLC instrument is Tianjin, island LC-10A type high performance liquid chromatograph, SPD-M10A type diode array UV-detector.Methanol is chromatographically pure, and water is ultra-pure water, and remaining solvent is analytical pure.
The selection of chromatographic condition and detection wavelength: ODS TSK-GEL (6.0mm * 150mm, 5 μ m); Methanol-water-glacial acetic acid (45: 54.45: 0.55) is a mobile phase; Flow velocity 0.8ml/min; The detection wavelength is 320nm; 25 ℃ of column temperatures.
Experiment material: get ferulic acid standard substance (purity is more than 99.9%) 5.0mg and add in the 25 μ l volumetric flasks, use methanol: formic acid (19: 1) standardize solution promptly gets reference substance solution.
System's precision is investigated: with same concentration sample, continuous sample introduction 5 times calculates content of ferulic acid (μ g/g), RSD=0.721%, and precision is good.
Repeatability is investigated: 5 parts of sample thiefs, and sample introduction, (the μ g/g) of content ferulic acid calculates, RSD=1.56%., repeatability good (table 2).
Study on the stability: with same concentration sample, 0,1,4,8,12,24 hours sample introductions calculate with content of ferulic acid (μ g/g), RSD=2.73%, and sample is stable.
The investigation of linear relationship:
Get the ferulic acid standard solution, respectively sample introduction 1,5,10,15,20,40 μ l.With peak area A the equation of linear regression that sample size M (μ g) obtains ferulic acid is respectively: M=8.00 * 10
-8A-0.116, r=99.9531%; The result shows that sample size and peak area are the good linear relation, and the range of linearity is 0.02-0.80 μ g.
Recovery test:
Precision takes by weighing the medical material sample, accurately adds a certain amount of ferulic acid, preparation test liquid, sample introduction analysis.The average recovery rate of ferulic acid is 100.00% as a result, RSD=0.73% (table 1).
(2) high pressure liquid chromatography of ligustilide (HPLC) quantitative analysis:
Instrument and reagent: Tianjin, island LC-10A type high performance liquid chromatograph, SPD-M10A type diode array UV-detector.Methanol is chromatographically pure, and water is ultra-pure water, and remaining solvent is analytical pure.
The recovery test of table 1 ferulic acid
| The suitable ferulaic acid content (μ g) of taking a sample | The amount (μ g) that adds ferulic acid | Measure the amount (μ g) of ferulic acid | The response rate (%) | Average recovery rate (%) | RSD (％) |
| 26.20 28.10 27.50 27.10 | 36.00 36.00 24.00 24.00 | 62.00 64.80 51.30 50.90 | 99.68 101.09 99.61 99.61 | 100.00 | 0.73 |
The selection of chromatographic condition and detection wavelength: ODS TSK-GEL (6.0mm * 150mm, 5 μ m); Methanol-water (40: 60) is a mobile phase; Flow velocity 0.7ml/min; The detection wavelength is 280nm; 25 ℃ of column temperatures.
Experiment material: get ligustilide standard substance (purity is more than 99%) 6.0mg and add in the 50ml volumetric flask,, promptly get reference substance solution with methanol-water (40: 60) standardize solution.
System's precision is investigated: with same concentration sample, and continuous sample introduction 5 times, the content of ligustilide (%) calculates, RSD=0.32%, precision is good.(table 1) repeatability is investigated: get wine and process 5 parts in Radix Angelicae Sinensis sample, sample introduction is with content (%) calculating of ligustilide, RSD=1.82%, repeatability good (table 2).
Study on the stability: with the concentration sample, 0,1,2,4,16,24 hours sample introductions are with content (%) calculating of ligustilide, RSD=3.95%., sample is stable.
The investigation of linear relationship: get the ligustilide standard solution, respectively sample introduction 1,2,4,6,8,10 μ l.With peak area A the equation of linear regression that sample size M (μ g) obtains ligustilide is respectively: M=3.00 * 10
-8A+0.029, r=99.9679%; The result shows that sample size and peak area are the good linear relation, and the range of linearity is 0.12-1.20 μ g.
Recovery test: precision takes by weighing wine and processs Radix Angelicae Sinensis medical material sample, accurately adds a certain amount of ligustilide, preparation test liquid, sample introduction analysis.The ligustilide average recovery rate is 98.63% as a result, RSD=1.05% (table 2).
The recovery test of table 2 ligustilide
| The suitable ligustilide content (μ g) of taking a sample | The amount (μ g) that adds ligustilide | Measure the amount (μ g) of ligustilide | The response rate (%) | Average recovery rate (%) | RSD (％) |
| 65.90 70.20 69.60 69.80 | 90.00 90.00 120.00 120.00 | 152.60 156.10 189.70 188.20 | 97.88 97.44 100.05 99.16 | 98.63 | 1.05 |
Conclusion: Radix Angelicae Sinensis wine of the present invention processs that the content of ferulic acid and ligustilide is respectively 0.0889 μ g and 0.3970 μ g in the decoction pieces.
Test example 2:
The comparison of the angelica sinensis processing procedure of tool function of promoting blood circulation to disperse blood clots is carried out by the following method.
(1) hit the variation of ferulic acid and ligustilide content before and after the skin in the subtractive process:
Get bright Radix Angelicae Sinensis 200 grams, wherein half adopts mechanical means to remove root bark, manually cuts into to return head and Radix Angelicae Sinensis.
Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 3):
Hit skin front and back ferulic acid and ligustilide cane sugar content measurement result in table 3 subtractive process
| Title | Weight (g) | The ferulic acid area | Suitable ferulic acid weight (ug) | Ferulaic acid content (%) | The ligustilide area | Suitable ligustilide weight (ug) | Ligustilide content (%) |
| Return a Radix Angelicae Sinensis epidermis peeling Radix Angelicae Sinensis peeling to return head | 1.51 1.50 1.52 1.50 1.50 | 231003 822748 509930 491207 160373 | 0.0304 0.0759 0.0519 0.0504 0.0249 | 0.0503 0.1266 0.0853 0.0840 0.0416 | 585266 1562308 1531422 275262 109984 | 0.49957 0.92947 0.91588 0.36317 0.29044 | 0.82710 1.54911 1.50638 0.60528 0.48407 |
The result shows, ferulic acid and ligustilide content all are higher than the peeling Radix Angelicae Sinensis and head is returned in peeling in the root bark.Histochemical studies shows that volatile ingredients such as ligustilide are stored in the grease chamber more, and ferulic acid etc. also mainly are distributed in phloem, therefore, in fact the removal of root bark has reduced the content of the secondary metabolite of Radix Angelicae Sinensis, has increased the content of sucrose etc. greatly.
(2) the section thickness is to the influence of ferulic acid and ligustilide content:
Get 3 batches of bright Radix Angelicae Sinensis,, be cut to sheet (thickness 2.5-4mm) respectively, thin slice (thickness 1.5-2.5mm), superthin section (thickness 1-1.5mm) according to concocting general survey in the pharmacopeia appendix.
Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 4):
The assay result of the different cutting thickness of table 4 sample ferulic acid and ligustilide
| Title | Weight (g) | The ferulic acid area | Be equivalent to ferulic acid weight (ug) | Ferulaic acid content (%) | The ligustilide area | Be equivalent to ligustilide weight (ug) | Ligustilide content (%) |
| Wafer thin thin slice sheet | 1.51 1.5 1.51 | 142294 434085 242007 | 0.02355 0.04601 0.03122 | 0.03898 0.07669 0.05169 | 92563 801212 206766 | 0.28278 0.59458 0.33303 | 0.46818 0.99097 0.55137 |
The result shows, thin slice is all high than the content of the ferulic acid of wafer thin and sheet and ligustilide.The thickness of decoction pieces, the variation of moisture and volatile ingredient when influence is dry on the one hand; The stripping of effective ingredient when influence is extracted on the one hand in addition.But decoction pieces is thin excessively, also may produce the absorption once more to composition.The comprehensive factor of putting is considered more, is advisable with thin slice.
(3) baking temperature is to the influence of ferulic acid and ligustilide content:
Get 4 batches of bright Radix Angelicae Sinensis, respectively with 35 degrees centigrade of dryings 5 hours; 40 degrees centigrade of dryings 4 hours; 50 degrees centigrade of dryings 4 hours; 50 degrees centigrade of dryings 4 hours.Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 5):
The assay result of table 5 different baking temperature sample ferulic acid and ligustilide
| Title | Weight (g) | The ferulic acid area | Be equivalent to ferulic acid weight (ug) | Ferulaic acid content (%) | The ligustilide area | Be equivalent to ligustilide weight (ug) | Ligustilide content (%) |
| 35 degree | 1.52 | 341384 | 0.03888 | 0.06394 | 536680 | 0.47819 | 0.78650 |
| 40 degree | 1.51 | 476511 | 0.04928 | 0.08159 | 1001607 | 0.68276 | 1.13039 |
| 50 degree | 1.53 | 433231 | 0.04595 | 0.07508 | 371470 | 0.40550 | 0.66258 |
| 60 degree | 1.50 | 440852 | 0.04653 | 0.07756 | 662915 | 0.53373 | 0.88956 |
The result shows that the ferulic acid of 40 ℃ of all the other temperature dryings of drying and the content of ligustilide are all high.Because Radix Angelicae Sinensis contains volatile ingredient and thermally labile component, temperature is too high, very easily destroys; Temperature is not enough, and moisture is higher again.The comprehensive factor of putting considers that baking temperature is advisable to be no more than 50 ℃ more.
(4) content of Radix Angelicae Sinensis different parts ferulic acid and ligustilide:
Get bright Radix Angelicae Sinensis, manual cutting is clip, main root (returning head), return body, return the body bottom, thick supporting root (Radix Angelicae Sinensis), several parts of ramuscule root (Radix Angelicae Sinensis).Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 6):
The assay result of table 6 different parts sample ferulic acid, ligustilide and sucrose
| Title | Weight (g) | The ferulic acid area | Be equivalent to ferulic acid weight (ug) | Ferulaic acid content (%) | The ligustilide area | Be equivalent to ligustilide weight (ug) | Ligustilide content (%) |
| The clip main root returns body to return body bottom thick supporting root ramuscule root | 1.49 1.51 1.52 1.49 1.50 1.51 | 342076 231003 500441 672216 822748 1028608 | 0.03893 0.03038 0.05112 0.06435 0.07594 0.09179 | 0.06532 0.05029 0.08408 0.10797 0.12657 0.15197 | 857868 585266 1147440 1268312 1562308 1994672 | 0.61951 0.49957 0.74692 0.80011 0.92947 1.11971 | 1.03945 0.82710 1.22849 1.34246 1.54911 1.85382 |
The result shows that the ferulic acid of main root and the content of ligustilide are minimum, and the content of ramuscule root is higher.This may be in traditional medication, " head hemostasis and up, body nourish blood and in keep, the tail removing blood stasis and dirty " empirical material base.But more the use of Radix Angelicae Sinensis at present is regardless of and returns head, Radix Angelicae Sinensis, adopts QUANGUI more.Still the standard of angelica sinensis is also only established QUANGUI.
(5) add the influence of capacity for liquor to ferulic acid and ligustilide content:
Get 3 batches of QUANGUI raw cooks, every batch 100 gram adds yellow wine according to 10%, 15%, 20% respectively, is vexedly inhaled to yellow wine, till the decoction pieces tool aroma.Put in the pot, with the decoction pieces parching to brown, taking-up is put cold with slow fire.
Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 7):
Table 7 difference adds the assay result that capacity for liquor is processed ferulic acid and ligustilide in the Radix Angelicae Sinensis sample
| Add capacity for liquor | Weight (g) | Suitable weight (μ g) | Ferulaic acid content (%) | Suitable weight (μ g) | Ligustilide content (%) |
| 10％ 15％ 20％ | 1.60 1.53 1.58 | 0.0848 0.0633 0.0589 | 0.1325 0.1034 0.0931 | 0.5493 0.3475 0.2820 | 0.8583 0.5678 0.4463 |
The result shows: when adding capacity for liquor 10%, the content of ferulic acid and ligustilide is the highest.An amount of with wine, can promote the stripping of composition, but it is too many to add capacity for liquor, also may make some ingredients from lossing.So adding capacity for liquor surely is 10%.
(6) parch temperature is to the influence of ferulic acid and ligustilide content:
Get 6 batches of QUANGUI raw cooks, every batch 100 gram adds yellow wine according to 10%, is vexedly inhaled to yellow wine, till the decoction pieces tool aroma.Put in the pot, use slow fire (35 ℃, 40 ℃, 45 ℃ and 50 ℃), moderate heat (65 ℃) and intense fire (80 ℃) with the decoction pieces parching to brown respectively, taking-up is put cold.
Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 8):
The different parch temperature of table 8 wine is processed the assay result of ferulic acid and ligustilide in the Radix Angelicae Sinensis sample
| The parch temperature | Example weight (g) | Suitable weight (μ g) | Ferulaic acid content (%) | Suitable weight (μ g) | Ligustilide content (%) |
| 80℃ 65℃ 50℃ 45℃ 40℃ 35℃ | 1.52 1.50 1.53 1.55 1.52 1.54 | 0.0676 0.0649 0.0889 0.0579 0.0535 0.0521 | 0.1112 0.1082 0.1453 0.0934 0.0881 0.0846 | 0.3174 0.2683 0.3970 0.4932 0.4813 0.5073 | 0.5220 0.4472 0.6487 0.7954 0.7916 0.8235 |
The result shows: in the time of 50 ℃, ferulaic acid content is the highest, and the content of ligustilide increases with temperature and reduces.General parch is grasped the duration and degree of heating according to experience, and parch has exsiccant effect concurrently.Temperature is too low, just needs the long time; The obvious zoom of decoction pieces that moderate heat and intense fire are fried, the extracting solution color is orange.We stipulate that the temperature of stir-fry is no more than 50 ℃.
(7) the parch time is to the influence of ferulic acid and ligustilide content:
Get 3 batches of QUANGUI raw cooks, every batch 100 gram adds yellow wine according to 10%, is vexedly inhaled to yellow wine, till the decoction pieces tool aroma.Put in the pot, fried 10 minutes, 20 minutes, 30 minutes with slow fire (about 50 ℃), taking-up is put cold.Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 9):
The result shows: the parch time increases, and ferulaic acid content increases, and the content of ligustilide reduces.The parch time, decoction pieces was pale brown color when being 20 minutes, and matter is pliable and tough, the tool wine aroma.The parch time, the decoction pieces color was darker when being 30 minutes, and matter is crisp firmly.In the actual plant produced,
The assay result of sample ferulic acid of different parch time of table 9 and ligustilide
| The parch time | Example weight (g) | Suitable weight (g) | Ferulaic acid content (%) | Suitable weight (g) | Ligustilide content (%) |
| 10 minutes 20 minutes 30 minutes | 1.51 1.51 1.55 | 0.0708 0.0763 0.0867 | 0.1171 0.1264 0.1399 | 0.4416 0.3882 0.3433 | 0.7312 0.6428 0.5538 |
It is bigger to throw the sample amount, parch time and stir frequency, stir number of times etc., all prepared slice quality is had considerable influence.So do not stipulate the concrete parch time that with the pale brown color of decoction pieces, matter is pliable and tough, water content is no more than 10%, and the tool wine aroma is degree.
(8) comparison of parch and baking:
Get 2 batches of QUANGUI raw cooks, every batch 100 gram adds yellow wine according to 10%, is vexedly inhaled to yellow wine, till the decoction pieces tool aroma.Put in the pot for 1 part, with the decoction pieces parching to brown, taking-up is put cold with slow fire (about 50 ℃); Put in the baking oven for other 1 part, extremely yellow with 50 ℃ of bakings.Content assaying method according to ferulic acid and ligustilide extracts and measures, result's following (table 10):
The assay result of table 10 parch and fired sample ferulic acid and ligustilide
| Title | Weight (g) | Suitable weight (μ g) | Ferulaic acid content (%) | Suitable weight (μ g) | Ligustilide content (%) |
| The parch oven dry | 1.50 1.50 | 0.0817 0.0617 | 0.1359 0.1027 | 0.4499 0.4459 | 0.7484 0.7427 |
The result shows: the ferulic acid of the decoction pieces of parch and the content of ligustilide are all than the height of drying.Chinese medicine as the instrument of concocting, not only plays heating with metalware, and the metallic element in the vessel etc. also have unique effect to acid ingredient etc., in addition, also play exsiccant effect in the time of the process of preparing Chinese medicine.So the process of preparing Chinese medicine of the angelica sinensis of tool blood circulation promoting and blood stasis dispelling only needs parch as a result, just can directly put coldly, pack.
Test example 3:
The pharmacological experiment of Wine-fried angelica decocting pieces of the present invention carries out by the following method.
(1) to the uterotonic inhibitory action of isolated rat:
Get female unpregnancy SD rat, body weight 180～210g tests preceding 2 days subcutaneous injection diethylstilbestrol 1mgkg
-1D
-1During experiment rat being taken off cervical vertebra puts to death, cut open the belly rapidly and take out the uterus, place the glass dish that fills pre-cooling Rockwell liquid immediately, carefully peel off the connective tissue and the fat that are attached on the Uterus wall, the uterus that the about 2cm of clip is long, two ends respectively with fill Rockwell bath of liquid groove in L shaped logical device hook link to each other with the muscular tension pick off, it is 37 ℃ ± 0.5 ℃ that water bath with thermostatic control keeps bath working solution temperature, continues to feed 95%O in the working solution
2After writing down one section normalized curve, add pituitrin (4.2u/L) and bring out uterine contraction, add behind the 10min and treat test agent, it is (inferior/as 10min), to calculate uterine contraction suppression ratio (%) to observe and write down interior uterine contraction amplitude (cm) of the forward and backward 10min of application of sample and frequency.
Found that, 1. Wine-fried angelica decocting pieces and granule thereof suppress the uterine smooth muscle contraction respectively greater than the Radix Angelicae Sinensis sheet and the Radix Angelicae Sinensis slice prescription granule of same concentrations, wherein Wine-fried angelica decocting pieces reaches 90.3% to the suppression ratio of intensity of uterine contraction, remarkable Radix Angelicae Sinensis sheet (P＜0.01) greater than same concentrations.2. two kinds of granules significantly are lower than Radix Angelicae Sinensis sheet and Wine-fried angelica decocting pieces, (0.125gL under the same concentrations condition to the inhibitory action of uterus smooth muscle contraction
-1), uterine contraction there is not obvious influence, concentration enlarges 10 times, and its inhibitory action still is lower than the latter respectively.
Four kinds of angelica sinensis are very not remarkable to the inhibitory action of uterus smooth muscle contraction frequency.Wherein, the Radix Angelicae Sinensis granule is to the inhibitory action of the contraction frequency Wine-fried angelica decocting pieces granule (table 11) greater than same concentrations.
The different angelica sinensis of concocting of table 11 bring out the uterotonic inhibitory action of isolated rat to pituitrin
| The sample title | Concentration (g/L) | Contraction intensity suppression ratio (%) | Contraction frequency suppression ratio (%) |
| Radix Angelicae Sinensis sheet Wine-fried angelica decocting pieces Radix Angelicae Sinensis slice prescription granule Wine-fried angelica decocting pieces granule | 0.125 0.125 0.125 1.25 0.125 1.25 | 53.3±4.6 90.3±2.2 a 0 43.3±5.8 0 50.4±5.2 b | 3.4±3.4 6.6±3.9 - 13.2±5.2 - 6.8±3.5 b |
N=6 x ± s is with Radix Angelicae Sinensis sheet ratio
aP＜0.01 is with Radix Angelicae Sinensis granule ratio
bP＜0.05
(2) analgesic activity of Wine-fried angelica decocting pieces test:
(A) the Radix Angelicae Sinensis sheet is to the analgesic activity (hot plate method) of mice
Select 50 of female ICR mices for use, body weight 18～21g is divided into 5 groups at random by body weight: the blank group, the basic, normal, high dosage group of Radix Angelicae Sinensis sheet (gives Radix Angelicae Sinensis sheet 0.9,1.8 and 3.6gkg respectively
-1D
-1) and positive controls (give aspirin 0.2gkg
-1D
-1).Each administration group is made into the finite concentration medicinal liquid to medicine with normal saline, press 0.2ml/10g body weight ig administration, once a day, continuous 5 days, the normal saline group is given the equivalent normal saline, and 1h carries out the hot plate method pain threshold and measures after the last administration: hot plate places more than 55 ℃ of water bath with thermostatic control preheating 10min, is placed on mice on the hot plate and picks up counting, mice is from being placed on the hot plate to the pain threshold that the metapedes required time is this Mus occurring licking, greater than 60 seconds exceeded with 60 seconds.The result shows, with normal saline group ratio, and the mice pain threshold effect that all is significantly increased of three dosage of Radix Angelicae Sinensis sheet, this effect is finite concentration and relies on trend, and its high dose group pain threshold is close with the aspirin group, shows that Radix Angelicae Sinensis Tabellae has significant analgesic activity.(table 12)
Table 12 Radix Angelicae Sinensis sheet is to the analgesic activity (hot plate method) of mice
| Group | Dosage (gkg -1·d -1) | Pain threshold (second) |
| Normal saline group Radix Angelicae Sinensis sheet aspirin | - 0.9 1.8 3.6 0.2 | 19.6±2.0 30.3±8.2 a 31.2±6.1 a 39.6±6.1 a 42.1±11.4 a |
N=10, x ± s is with normal saline group ratio
aP＜0.01
(B) four kinds of angelica sinensis analgesic activities relatively
On Radix Angelicae Sinensis sheet analgesic activity experiment basis, dosage carries out four kinds of decoction pieces analgesic activities relatively in the selection.
(I) hot plate method: the results are shown in Table 13
By table 13 as seen, four angelica sinensis group pain thresholds show that all significantly greater than the normal saline group four kinds of angelica sinensis all have significant analgesic activity; In four kinds of decoction pieces, Wine-fried angelica decocting pieces group pain threshold is greater than other each groups, but there was no significant difference (P＞0.05) between group.
Four kinds of angelica sinensis analgesic activities of table 13 are (hot plate method) relatively
| Group | Dosage (gkg -1·d -1) | Number of animals (only) | Pain threshold (second) |
| Normal saline Radix Angelicae Sinensis sheet Wine-fried angelica decocting pieces Radix Angelicae Sinensis slice prescription granule Wine-fried angelica decocting pieces granule aspirin | - 1.8 1.8 1.8 * 1.8 * 0.2 | 15 16 14 14 14 13 | 19.±1.9 32.0±6.6 a 43.6±14.0 a 33.4±6.3 a 37.6±15.6 a 41.5±14.5 a |
X ± s is with normal saline group ratio
aP＜0.01
*In contained crude drug amount
(II) mouse peritoneal injection acetic acid is caused 72 of the analgesic activity one writhing method ICR mices of pain, ♀ ♂ half and half, body weight 18～21g, be divided into 6 groups at random, grouping and the same hot plate method of administration, successive administration 5 days, 1 hour ip 0.6% acetic acid 0.2m1 after the last administration, count and turn round the body number of times in every Mus 15min, between organizing relatively.The result is similar to hot plate method, and four kinds of angelica sinensis all can significantly reduce turns round the body number of times behind the mouse peritoneal injection acetic acid, shows to have significant analgesic activity.In four kinds of decoction pieces, the analgesic activity of wine Wine-fried angelica decocting pieces and two kinds of granules is significantly greater than the Radix Angelicae Sinensis sheet, but do not have significant difference (table 14) between the three.
Four kinds of angelica sinensis analgesic activities of table 14 are (writhing method) relatively
| Group | Dosage (gkg -1·d -1) | Number of animals (only) | Turn round body number of times/15min |
| Normal saline Radix Angelicae Sinensis sheet Wine-fried angelica decocting pieces Radix Angelicae Sinensis granule Wine-fried angelica decocting pieces granule aspirin | - 1.8 1.8 1.8* 1.8* 0.2 | 12 12 12 12 12 12 | 90.8±12.9 75.8±10.5 a 52.4±11.9 ab 56.2±15.6 ab 55.5±10.8 ab 33.2±5.4 ab |
X ± s is with normal saline group ratio
aP＜0.01 is with Radix Angelicae Sinensis sheet group ratio
bP＜0.01
*In contained crude drug amount
(C) four kinds of angelica sinensis are to the influence of platelet aggregation:
A) platelet rich plasma (platelet-rich plasma, PRP) and platelet poor plasma (platelet-poor plasma, PPP) preparation: clear-headed certainly rabbit carotid artery is got blood and is collected in the plastic centrifuge tube, with 3.8% sodium citrate anticoagulant (blood and anticoagulant volume ratio 9: 1).The centrifugal 10min of 1000rpm under the room temperature gets PRP, and sucking-off PRP is in clean plastic tube; Residue blood gets PPP with the centrifugal 10min of 3000rpm again.PPP is used for returning to zero or adjusts the number of platelets of PRP, and platelet count is controlled at 5 * 10 in the process of the test
11Cell/L.
B) platelet aggregation rate is measured
With of the influence of four kinds of angelica sinensis variable concentrations of Born ' s turbidimetry for Determination medicinal liquid to ADP, PAF and the inductive platelet aggregation of AA.Be about to medicinal liquid and PRP in 37 ℃ of incubation 5min, add different derivants, measure 5min platelet maximum agglutination rate (%), (control tube is the Polyethylene Glycol of final concentration 0.6%) is calculated as follows platelet aggregation inhibition rate (%):
The result shows that four kinds of angelica sinensis all have the anticoagulant effect, and this effect is tangible dose-effect relationship.To ADP and the inductive platelet aggregation of PAF, Wine-fried angelica decocting pieces and Wine-fried angelica decocting pieces granule inhibitory action are significantly greater than Radix Angelicae Sinensis sheet and Radix Angelicae Sinensis granule, the effect of Wine-fried angelica decocting pieces granule anticoagulant is lower than Wine-fried angelica decocting pieces, but the two no significant difference.Four kinds of angelica sinensis are to the accumulative inhibitory action no significant difference of AA induced platelet (table 15).
Results of pharmacodynamic test shows, Wine-fried angelica decocting pieces suppresses the physiologically active of uterine smooth muscle contraction, analgesia and antiplatelet aggregation three aspects all significantly greater than the Radix Angelicae Sinensis sheet, shows that the angelica sinensis of gained of the present invention has the merit of good blood circulation promoting and blood stasis dispelling, menstruction regulating and pain relieving.Can be used for menoxenia, blood deficiency and yellow complexion, dizzy cardiopalmus, comprehensive card waited gynecologic blood diseases or can be used as the crude drug use of gynecologic blood diseases compound preparation climacteric.
Part results of pharmacodynamic test to four kinds of angelica sinensis preparations shows; Wine-fried angelica decocting pieces suppresses the physiologically active of uterine smooth muscle contraction, analgesia and antiplatelet aggregation three aspects all significantly greater than the Radix Angelicae Sinensis sheet; remove analgesic activity with; outside the Wine-fried angelica decocting pieces granule basically identical; other effects also are better than the latter; therefore, Wine-fried angelica decocting pieces may be the first-selected preparation of the corresponding disease of clinical treatment.
The external inhibitory action of ADP, PAF, AA being induced the rabbit platelet aggregation of table 15 angelica sinensis
| Platelet aggregation inhibition rate (%) | |||||||||
| ADP(3μmol·L -1) | PAF(7.2nmol·L -1) | AA(0.35mmol·L -1) | |||||||
| 0.3 | 0.6 | 0.9 | 0.3 | 0.6 | 0.9 | 0.3 | 0.6 | 0.9 | |
| (g·L -1) | (g·L -1) | (g·L -1) | |||||||
| Radix Angelicae Sinensis sheet Wine-fried angelica decocting pieces Radix Angelicae Sinensis granule Wine-fried angelica decocting pieces granule aspirin (final concentration mmolL -1) | 17.4±6.3 24.9±5.1 17.5±9.2 23.1±4.6 30.4±5.0 (1.85) | 32.5±7.5 36.8±5.9 29.9±8.0 30.9±10.1 43.4±0.9 (3.7) | 41.6±6.9 53.6±8.6 b 40.1±10.0 54.8±6.6 bc 64.6±2.5 (5.55) | 11.9±5.2 24.2±9.7 b 9.9±3.1 20.6±7.6 c 22.1±1.0 (1.85) | 25.0±4.8 48.2±8.8 bc 31.9±2.9 44.0±5.4 c 42.4±4.8 (3.70) | 50.1±8.1 79.3±3.9 ad 59.3±7.4 68.9±6.2 c 63.7±0.9 (5.55) | 12.1±6.2 14.2±5.6 16.3±6.1 18.3±7.4 30.9±6.9 (1.85) | 41.9±8.7 37.8±5.9 33.1±3.9 37.2±5.1 79.5±2.9 (3.70) | 57.4±8.6 51.7±8.0 50.6±6.8 51.8±8.7 85.4±0.2 (5.55) |
N=4, x ± s is with Radix Angelicae Sinensis sheet ratio
aP＜0.01
bP＜0.05 is with Radix Angelicae Sinensis granule ratio
cP＜0.05,
dP＜0.01
Drawing the present invention's superiority compared with prior art from the above embodiments with the test example is:
1, by technical scheme of the present invention concoct angelica sinensis, the physiologically active that suppresses uterine smooth muscle contraction, analgesia and antiplatelet aggregation three aspects is all significantly greater than the Radix Angelicae Sinensis sheet.
2, concocting process of the present invention is simple, is easy to quality control and suitability for industrialized production.
3, the processing of traditional Chinese angelica method that is applied as of the inventive method provides normalization procedure.
Claims (7)
1. a Wine-fried angelica decocting pieces, wherein ferulic acid (C
10H
10O
4) and the content of ligustilide be no less than 0.050% respectively, the content of ligustilide is no less than 0.080%.
2. Wine-fried angelica decocting pieces, to make as follows when being classified as raw material:
The Radix Angelicae Sinensis of root bark, remove impurity, rinsing will do not removed, soak into, cutting is the thin slice of thickness 1.7-2.7mm, and baking temperature is no more than and dries under 45 ℃ or dry, add the yellow wine of 10% proportional quantities, mix thoroughly, vexed, inhaled profit 5-7 hour, and exhausted to yellow wine, till the decoction pieces tool aroma, put in the pot, be no more than 50 ℃ with temperature, time 50-60 minute slow fire fries decoction pieces to buff, the tool aroma is taken out, and is lower than 19% to the decoction pieces water content and puts cold getting final product.
3. the preparation method of claim 1 or 2 Wine-fried angelica decocting pieces, the Radix Angelicae Sinensis of root bark will do not removed, remove impurity, rinsing is soaked into, cutting is the thin slice of thickness 1.7-2.7mm, baking temperature is no more than and dries under 45 ℃ or dry, and adds the yellow wine of 10% proportional quantities, mixes thoroughly, vexed, inhaled profit 5-7 hour, and exhausted to yellow wine, till the decoction pieces tool aroma, put in the pot, be no more than 50 ℃ with temperature, time 50-60 minute slow fire fries decoction pieces to buff, the tool aroma, take out, be lower than 19% to the decoction pieces water content and put cold getting final product.
4. Wine-fried angelica decocting pieces granule is that raw material makes as follows with the Radix Angelicae Sinensis (processed with wine) sheet:
Decoct with water, adding drinking-water for the first time and be 10 times of medical material amounts, is 8 times for the second time, is 6 times for the second time, before for the first time opening steam, soaked into 30 minutes earlier, pick up counting, be respectively 2 hours, 1.5 hours and 1.5 hours for 3 times with boiling, merge extractive liquid,, concentrate below 80 ℃,, get extractum to relative density 1.37-1.40 (80 ℃); Get lactose and mannitol,, get adjuvant according to mixing in 1: 1; With extractum: adjuvant 1: the 5-6 mixed is granulated to appropriateness, crosses 20 mesh sieves, and 50 ℃ of dryings are crossed 20 mesh sieve granulate, and 50 ℃ of dryings 30 minutes promptly get the Wine-fried angelica decocting pieces granule of moisture 6%.
5. claim 1 or 2 or 4 described Wine-fried angelica decocting pieces or Wine-fried angelica decocting pieces granule are returned application in the medicine of section's mass formed by blood stasis in preparation treatment or prevention.
6. claim 1 or 2 or 4 described Wine-fried angelica decocting pieces or the Wine-fried angelica decocting pieces granule application in the preparation medicament for resisting platelet aggregation.
7. claim 1 or 2 or 4 described Wine-fried angelica decocting pieces or the Wine-fried angelica decocting pieces granule application in the preparation analgesic.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028750A (en) * | 2010-12-01 | 2011-04-27 | 四川农业大学 | Processing method of traditional Chinese medicine Salvia miltiorrhiza Bunge |
| CN105816493A (en) * | 2014-09-17 | 2016-08-03 | 昆明中如农业科技有限公司 | Chinese angelica extract and preparation method thereof |
| CN108245540A (en) * | 2018-04-04 | 2018-07-06 | 安徽颐生堂中药饮片有限公司 | A kind of preparation method of angelica sinensis |
| CN114848693A (en) * | 2022-04-22 | 2022-08-05 | 陕西科技大学 | Wine-washed angelica sinensis decoction pieces and processing method, quality detection method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1059343C (en) * | 1997-01-16 | 2000-12-13 | 北京市中药综合研究所 | Chinese medicine dried pieces of fresh rehmannia root and its preparation process |
| CN1857437A (en) * | 2006-03-17 | 2006-11-08 | 中国科学院长春应用化学研究所 | Method for processing Chinese medicine white peony root |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102028750A (en) * | 2010-12-01 | 2011-04-27 | 四川农业大学 | Processing method of traditional Chinese medicine Salvia miltiorrhiza Bunge |
| CN105816493A (en) * | 2014-09-17 | 2016-08-03 | 昆明中如农业科技有限公司 | Chinese angelica extract and preparation method thereof |
| CN108245540A (en) * | 2018-04-04 | 2018-07-06 | 安徽颐生堂中药饮片有限公司 | A kind of preparation method of angelica sinensis |
| CN114848693A (en) * | 2022-04-22 | 2022-08-05 | 陕西科技大学 | Wine-washed angelica sinensis decoction pieces and processing method, quality detection method and application thereof |
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Assignee: Yunnan Weihe Pharmaceutical Co., Ltd. Assignor: Kunming Institute of Botany, Chinese Academy of Sciences Contract record no.: 2010530000050 Denomination of invention: Insulation material for heating wire, method for making same and use thereof Granted publication date: 20100519 License type: Exclusive License Open date: 20080312 Record date: 20100929 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100519 Termination date: 20160830 |
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