CN101120098A - 核酸分析方法 - Google Patents

核酸分析方法 Download PDF

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Publication number
CN101120098A
CN101120098A CNA2005800191648A CN200580019164A CN101120098A CN 101120098 A CN101120098 A CN 101120098A CN A2005800191648 A CNA2005800191648 A CN A2005800191648A CN 200580019164 A CN200580019164 A CN 200580019164A CN 101120098 A CN101120098 A CN 101120098A
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China
Prior art keywords
nucleic acid
nucleotide
reaction mixture
labeled
template
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Pending
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CNA2005800191648A
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English (en)
Chinese (zh)
Inventor
C·W·福勒
J·R·纳尔逊
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Global Life Sciences Solutions USA LLC
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Amersham Biosciences Corp
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Publication of CN101120098A publication Critical patent/CN101120098A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNA2005800191648A 2004-06-10 2005-06-09 核酸分析方法 Pending CN101120098A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57878904P 2004-06-10 2004-06-10
US60/578,789 2004-06-10

Publications (1)

Publication Number Publication Date
CN101120098A true CN101120098A (zh) 2008-02-06

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ID=35510340

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2005800191648A Pending CN101120098A (zh) 2004-06-10 2005-06-09 核酸分析方法

Country Status (9)

Country Link
US (1) US7871771B2 (https=)
EP (1) EP1766075B1 (https=)
JP (1) JP5037343B2 (https=)
CN (1) CN101120098A (https=)
AT (1) ATE461293T1 (https=)
AU (1) AU2005254984B2 (https=)
CA (1) CA2567591C (https=)
DE (1) DE602005020011D1 (https=)
WO (1) WO2005123957A2 (https=)

Cited By (15)

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CN103717753A (zh) * 2011-05-27 2014-04-09 吉纳普赛斯股份有限公司 用于遗传和生物分析的系统和方法
US9150915B2 (en) 2010-10-04 2015-10-06 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US9274077B2 (en) 2011-05-27 2016-03-01 Genapsys, Inc. Systems and methods for genetic and biological analysis
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9434983B2 (en) 2011-05-27 2016-09-06 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
US9945807B2 (en) 2010-10-04 2018-04-17 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
WO2018121587A1 (zh) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法
US10093975B2 (en) 2011-12-01 2018-10-09 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
US10125393B2 (en) 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
CN109790578A (zh) * 2016-10-05 2019-05-21 豪夫迈·罗氏有限公司 使用纳米晶体管的核酸测序
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
US10900075B2 (en) 2017-09-21 2021-01-26 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CN114555832A (zh) * 2019-05-27 2022-05-27 通用测序技术公司 鉴定核酸序列中组分的方法

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US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
WO2010141391A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
WO2013096692A1 (en) 2011-12-21 2013-06-27 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
EP2844774B1 (en) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna sequencing
EP2844775B1 (en) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna sequencing
WO2013166303A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
KR102254451B1 (ko) * 2016-04-22 2021-05-20 옴니옴 인코포레이티드 뉴클레오타이드-특이적 삼원 복합체 형성의 향상된 검출을 사용하는 핵산 서열 결정 방법 및 시스템
CA3022431C (en) 2016-04-29 2023-03-21 Omniome, Inc. Method of nucleic acid sequence determination
EP3449012B1 (en) 2016-04-29 2020-12-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
KR102230444B1 (ko) 2016-08-15 2021-03-23 옴니옴 인코포레이티드 핵산을 시퀀싱하기 위한 방법 및 시스템
US11248254B2 (en) 2016-12-30 2022-02-15 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
US10975427B2 (en) 2017-01-20 2021-04-13 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10737267B2 (en) 2017-04-04 2020-08-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2019079593A1 (en) 2017-10-19 2019-04-25 Omniome, Inc. BACKGROUND NOISE REDUCTION AND STABILIZATION OF SIMULTANEOUS COMPLEXES IN LINKED ASSAY WORKFLOWS
CN112567048B (zh) 2018-07-24 2026-01-23 加利福尼亚太平洋生物科学股份有限公司 三元复合物种类的连续形成
EP3853358A1 (en) 2018-09-17 2021-07-28 Omniome, Inc. Engineered polymerases for improved sequencing
GB2613480B (en) 2018-11-15 2023-11-22 Element Biosciences Inc Methods for generating circular nucleic acid molecules
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
WO2021092431A1 (en) 2019-11-08 2021-05-14 Omniome, Inc. Engineered polymerases for improved sequencing by binding
CA3178970A1 (en) * 2020-05-20 2021-11-25 Sinan ARSLAN Methods for paired-end sequencing library preparation

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WO1998045474A1 (en) * 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
ATE371022T1 (de) * 1998-06-17 2007-09-15 Ge Healthcare Bio Sciences Fy7 polymerase
CN1197975C (zh) * 1998-08-19 2005-04-20 生物风险公司 测定多核苷酸序列变异的方法
GB9901475D0 (en) * 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US20030108867A1 (en) 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6274320B1 (en) * 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
EP1368497A4 (en) * 2001-03-12 2007-08-15 California Inst Of Techn METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis

Cited By (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9945807B2 (en) 2010-10-04 2018-04-17 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9150915B2 (en) 2010-10-04 2015-10-06 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US9187783B2 (en) 2010-10-04 2015-11-17 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US10539527B2 (en) 2010-10-04 2020-01-21 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods for detecting or analyzing a sample
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US10472674B2 (en) 2010-10-04 2019-11-12 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US10100356B2 (en) 2010-10-04 2018-10-16 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US9533305B2 (en) 2010-10-04 2017-01-03 Genapsys, Inc. Systems and methods for automated reusable parallel biological reactions
US10787705B2 (en) 2011-05-27 2020-09-29 Genapsys, Inc. Systems and methods for genetic and biological analysis
US10494672B2 (en) 2011-05-27 2019-12-03 Genapsys, Inc. Systems and methods for genetic and biological analysis
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US11155865B2 (en) 2011-05-27 2021-10-26 Genapsys, Inc. Systems and methods for genetic and biological analysis
US11021748B2 (en) 2011-05-27 2021-06-01 Genapsys, Inc. Systems and methods for genetic and biological analysis
US10059982B2 (en) 2011-05-27 2018-08-28 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
CN103717753A (zh) * 2011-05-27 2014-04-09 吉纳普赛斯股份有限公司 用于遗传和生物分析的系统和方法
CN103717753B (zh) * 2011-05-27 2016-12-07 吉纳普赛斯股份有限公司 用于遗传和生物分析的系统和方法
US10612091B2 (en) 2011-05-27 2020-04-07 Genapsys, Inc. Systems and methods for genetic and biological analysis
US10260095B2 (en) 2011-05-27 2019-04-16 Genapsys, Inc. Systems and methods for genetic and biological analysis
US10266892B2 (en) 2011-05-27 2019-04-23 Genapsys, Inc. Systems and methods for genetic and biological analysis
US9274077B2 (en) 2011-05-27 2016-03-01 Genapsys, Inc. Systems and methods for genetic and biological analysis
US9434983B2 (en) 2011-05-27 2016-09-06 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US10093975B2 (en) 2011-12-01 2018-10-09 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
US11286522B2 (en) 2011-12-01 2022-03-29 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
US10570449B2 (en) 2013-03-15 2020-02-25 Genapsys, Inc. Systems and methods for biological analysis
US10125393B2 (en) 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
US10533218B2 (en) 2014-04-18 2020-01-14 Genapsys, Inc. Methods and systems for nucleic acid amplification
US11332778B2 (en) 2014-04-18 2022-05-17 Genapsys, Inc. Methods and systems for nucleic acid amplification
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CN109790578A (zh) * 2016-10-05 2019-05-21 豪夫迈·罗氏有限公司 使用纳米晶体管的核酸测序
CN109790578B (zh) * 2016-10-05 2024-04-09 豪夫迈·罗氏有限公司 使用纳米晶体管的核酸测序
WO2018121587A1 (zh) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 一种基于单荧光染料的测序方法
US11466318B2 (en) 2016-12-27 2022-10-11 Egi Tech (Shen Zhen) Co., Limited Single fluorescent dye-based sequencing method
US10900075B2 (en) 2017-09-21 2021-01-26 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CN114555832A (zh) * 2019-05-27 2022-05-27 通用测序技术公司 鉴定核酸序列中组分的方法

Also Published As

Publication number Publication date
EP1766075A2 (en) 2007-03-28
WO2005123957A3 (en) 2006-05-18
DE602005020011D1 (de) 2010-04-29
CA2567591A1 (en) 2005-12-29
CA2567591C (en) 2011-08-02
US7871771B2 (en) 2011-01-18
WO2005123957A2 (en) 2005-12-29
ATE461293T1 (de) 2010-04-15
US20080287305A1 (en) 2008-11-20
EP1766075B1 (en) 2010-03-17
AU2005254984A1 (en) 2005-12-29
JP5037343B2 (ja) 2012-09-26
JP2008502369A (ja) 2008-01-31
EP1766075A4 (en) 2008-05-21
AU2005254984B2 (en) 2008-10-16

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Application publication date: 20080206