AU2005254984B2 - Method for nucleic acid analysis - Google Patents

Method for nucleic acid analysis Download PDF

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Publication number
AU2005254984B2
AU2005254984B2 AU2005254984A AU2005254984A AU2005254984B2 AU 2005254984 B2 AU2005254984 B2 AU 2005254984B2 AU 2005254984 A AU2005254984 A AU 2005254984A AU 2005254984 A AU2005254984 A AU 2005254984A AU 2005254984 B2 AU2005254984 B2 AU 2005254984B2
Authority
AU
Australia
Prior art keywords
nucleic acid
reaction mixture
labeled
template
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2005254984A
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English (en)
Other versions
AU2005254984A1 (en
Inventor
Carl W. Fuller
John R. Nelson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences Corp
Publication of AU2005254984A1 publication Critical patent/AU2005254984A1/en
Application granted granted Critical
Publication of AU2005254984B2 publication Critical patent/AU2005254984B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2005254984A 2004-06-10 2005-06-09 Method for nucleic acid analysis Ceased AU2005254984B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US57878904P 2004-06-10 2004-06-10
US60/578,789 2004-06-10
PCT/US2005/020378 WO2005123957A2 (en) 2004-06-10 2005-06-09 Method for nucleic acid analysis

Publications (2)

Publication Number Publication Date
AU2005254984A1 AU2005254984A1 (en) 2005-12-29
AU2005254984B2 true AU2005254984B2 (en) 2008-10-16

Family

ID=35510340

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005254984A Ceased AU2005254984B2 (en) 2004-06-10 2005-06-09 Method for nucleic acid analysis

Country Status (9)

Country Link
US (1) US7871771B2 (https=)
EP (1) EP1766075B1 (https=)
JP (1) JP5037343B2 (https=)
CN (1) CN101120098A (https=)
AT (1) ATE461293T1 (https=)
AU (1) AU2005254984B2 (https=)
CA (1) CA2567591C (https=)
DE (1) DE602005020011D1 (https=)
WO (1) WO2005123957A2 (https=)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
WO2010141390A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
KR101940833B1 (ko) * 2011-05-27 2019-01-21 제납시스 인크. 유전자 및 생물학적 분석을 위한 시스템 및 방법
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CN106591103B (zh) 2011-12-01 2021-06-04 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
WO2013096692A1 (en) 2011-12-21 2013-06-27 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
EP3783111A1 (en) 2012-05-02 2021-02-24 Ibis Biosciences, Inc. Dna sequencing
EP2844774B1 (en) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna sequencing
US10202642B2 (en) 2012-05-02 2019-02-12 Ibis Biosciences, Inc. DNA sequencing
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
EP3556864B1 (en) 2014-04-18 2020-12-09 Genapsys, Inc. Methods and systems for nucleic acid amplification
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
KR102254451B1 (ko) * 2016-04-22 2021-05-20 옴니옴 인코포레이티드 뉴클레오타이드-특이적 삼원 복합체 형성의 향상된 검출을 사용하는 핵산 서열 결정 방법 및 시스템
EP3449012B1 (en) 2016-04-29 2020-12-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
AU2017258523B2 (en) * 2016-04-29 2020-08-13 Pacific Biosciences Of California, Inc. Method of Nucleic Acid sequence determination
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
EP3497236B1 (en) 2016-08-15 2021-11-03 Omniome, Inc. Method for sequencing nucleic acids
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
US11345961B2 (en) * 2016-10-05 2022-05-31 Roche Sequencing Solutions, Inc. Nucleic acid sequencing using nanotransistors
RU2760737C2 (ru) * 2016-12-27 2021-11-30 Еги Тек (Шэнь Чжэнь) Ко., Лимитед Способ секвенирования на основе одного флуоресцентного красителя
EP3562962B1 (en) 2016-12-30 2022-01-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
WO2018136487A1 (en) 2017-01-20 2018-07-26 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
EP3697932A1 (en) 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
WO2020023362A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
EP3853358A1 (en) 2018-09-17 2021-07-28 Omniome, Inc. Engineered polymerases for improved sequencing
WO2020102766A2 (en) 2018-11-15 2020-05-22 Element Biosciences, Inc. Methods for generating circular nucleic acid molecules
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
CN114555832A (zh) * 2019-05-27 2022-05-27 通用测序技术公司 鉴定核酸序列中组分的方法
EP4055155A1 (en) 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Engineered polymerases for improved sequencing by binding
WO2021236792A1 (en) * 2020-05-20 2021-11-25 Element Biosciences, Inc. Methods for paired-end sequencing library preparation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020164629A1 (en) * 2001-03-12 2002-11-07 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998045474A1 (en) * 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
ATE371022T1 (de) * 1998-06-17 2007-09-15 Ge Healthcare Bio Sciences Fy7 polymerase
IL141329A (en) * 1998-08-19 2005-07-25 Bioventures Inc Method for detemining polynucleotides sequence variations
GB9901475D0 (en) * 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US20030108867A1 (en) * 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6274320B1 (en) * 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020164629A1 (en) * 2001-03-12 2002-11-07 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension

Also Published As

Publication number Publication date
DE602005020011D1 (de) 2010-04-29
EP1766075A2 (en) 2007-03-28
JP5037343B2 (ja) 2012-09-26
ATE461293T1 (de) 2010-04-15
EP1766075B1 (en) 2010-03-17
WO2005123957A2 (en) 2005-12-29
JP2008502369A (ja) 2008-01-31
AU2005254984A1 (en) 2005-12-29
CA2567591C (en) 2011-08-02
US20080287305A1 (en) 2008-11-20
CN101120098A (zh) 2008-02-06
EP1766075A4 (en) 2008-05-21
US7871771B2 (en) 2011-01-18
WO2005123957A3 (en) 2006-05-18
CA2567591A1 (en) 2005-12-29

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MK14 Patent ceased section 143(a) (annual fees not paid) or expired