CN101085978B - Filtering-precipitation dual perfusion system for animal cell perfusion culture - Google Patents
Filtering-precipitation dual perfusion system for animal cell perfusion culture Download PDFInfo
- Publication number
- CN101085978B CN101085978B CN2007100288536A CN200710028853A CN101085978B CN 101085978 B CN101085978 B CN 101085978B CN 2007100288536 A CN2007100288536 A CN 2007100288536A CN 200710028853 A CN200710028853 A CN 200710028853A CN 101085978 B CN101085978 B CN 101085978B
- Authority
- CN
- China
- Prior art keywords
- perfusion
- peristaltic pump
- filtering
- precipitation
- inner prop
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses one filteration- deposition combined perfusion system used for animal cell perfusion culture. It comprises filtering- deposition- perfusion column, peristaltic pump, automatic controller, tri- way pipeline and pipe clamp; filtering- deposition- perfusion column is connected with peristaltic pump through tri- way pipeline, the rotation of peristaltic pump is controlled by automatic controller, pipe clamp is installed on pipe between filtering- deposition- perfusion column and peristaltic pump. The invention is characterized by simple structure, low cost, high collecting efficiency, long life length and wide application on micro- carrier or cluster animal cell perfusion culture.
Description
Technical field
The present invention relates to a kind of filtering-precipitation double combination perfusion system that the zooblast perfusion is cultivated that is used for, belong to cell engineering field and field of biological pharmacy.
Background technology
Kind with zooblast producer gene engineering medicine is more and more, the particularly appearance of engineered antibody, and output has become " bottleneck " of production.With perfused culture system substitute traditional batch or batch-production technique added more and more causes people's attention.Adopt perfused culture system can improve cell density and output greatly, significantly reduce the investment in equipment and delivery room.Cultivate in order to carry out effective perfusion, but its core be to have a cover high-level efficiency, low cost, and life-time service with the perfusion system of cell retention in retort.The device for trapping of existing so far report has built-in or external placed type strainer, sedimentation device, tubular fibre, ultrasonic filter, whizzer etc., have in these device for trapping to hold back efficient cost limited, that have not long duration of service very high, that have, all also there is some deficiency, can not satisfies each side's needs.
Summary of the invention
A nested structure is simple, cost is low, hold back efficient height life-time service but the purpose of this invention is to provide, and is applicable to the microcarrier of zooblast or the filtering-precipitation double combination perfusion system that cluster perfusion is cultivated.
Purpose of the present invention is achieved by taking following technical scheme:
A kind of filtering-precipitation double combination perfusion system that is used for the cultivation of zooblast perfusion, comprise the two-in-one perfusion post of filtering-precipitation, peristaltic pump, two-position controller, three-way pipeline and pipeline folder, the two-in-one perfusion post of described filtering-precipitation is connected with peristaltic pump by three-way pipeline, and peristaltic pump is by two-position controller control running, and the pipeline folder is arranged on the pipeline that is connected between the two-in-one perfusion post of filtering-precipitation and the peristaltic pump.
The two-in-one perfusion post of filtering-precipitation of the present invention comprises inner prop and overcoat, inner prop is enclosed within the overcoat, wherein inner prop is made up of filter screen, support and the drain pipe in 20～70 μ m apertures, overcoat is made up of loam cake, sleeve and drain pipe, the drain pipe of inner prop places the upper end of inner prop and overcoat respectively with the drain pipe of overcoat and links to each other with the drawing liquid pipeline, and the two-in-one perfusion post of filtering-precipitation places in the retort.
Peristaltic pump of the present invention is a peristaltic pump can long-term operation, forward and reverse rotation, comprises drawing liquid peristaltic pump and reinforced peristaltic pump, and the drawing liquid peristaltic pump is connected with the drawing liquid jar, and reinforced peristaltic pump is connected with feed pot.
Two-position controller of the present invention is can orientation and the electronic digit two-position controller of timing control peristaltic pump sense of rotation and time.
The inner prop of the two-in-one perfusion post of filtering-precipitation of the present invention is connected in feed pot by three-way pipeline, and is used for the filter screen of Artificial Control fresh culture flushing inner prop by pipeline folder trip switch.
Technical characterstic of the present invention is:
(1) cultivate the initial stage when the bio-reactor inner cell at microcarrier or conglomeration, have the part cell also not enter carrier, or also do not form enough big conglomeration, the filter screen inner prop in the perfusion post can play crown_interception to single or cell nodule mass;
(2) for avoiding cell to stop up filter screen, control peristaltic pump rotating direction and the electronic digit two-position controller of time can make peristaltic pump interval type ground will sweep away attached to the cell on the filter screen with the supernatant of results;
(3) for strengthening the efficient that sweeps away cell on the filter screen, utilize three-way pipeline and the pipeline folder that is switchable to fresh culture, every day, the filter screen of inner prop was washed in 1～2 short period of time artificially with fresh culture;
(4) enter the carrier of cell or bigger cell conglomeration, proportion is bigger, and perfusion post overcoat can dam it in the telescopic bottom, thereby has alleviated the filtration load of filter screen inner prop and the probability of stopping up;
(5) when cell density very high, when carrier or cell conglomeration proportion are very big, results supernatant can be without the filter screen inner prop, directly receive liquid through the liquid outlet of sedimentation overcoat, same, when cell perfusion incubation time long, during the part cell senescence, also can be in order to removable parts carrier and cell.
In sum, but that the present invention has is simple in structure, cost is low, hold back characteristics such as efficient height life-time service, and the microcarrier or the cluster perfusion that can be widely used in zooblast are cultivated.
Description of drawings
Fig. 1 is the two-in-one perfusion rod structure of a filtering-precipitation of the present invention synoptic diagram;
Fig. 2 is the concrete implementing procedure synoptic diagram of perfusion system of the present invention.
Embodiment
As shown in Figure 1, 2, a kind of filtering-precipitation double combination perfusion system that the zooblast perfusion is cultivated that is used for provided by the invention, comprise the two-in-one perfusion post of filtering-precipitation, peristaltic pump 5,6, two-position controller 9, three-way pipeline 10 and pipeline folder 12, the two-in-one perfusion post of described filtering-precipitation is connected with peristaltic pump 5,6 by three-way pipeline 10, peristaltic pump 5,6 is by two-position controller 9 control running, and pipeline folder 12 is arranged on the pipeline that is connected between the two-in-one perfusion post of filtering-precipitation and the peristaltic pump.The two-in-one perfusion post of filtering-precipitation comprises inner prop 1 and overcoat 2, inner prop 1 is enclosed within the overcoat 2, wherein inner prop 1 is made up of filter screen 11, support 14 and the drain pipe 13 in 20～70 μ m apertures, the material of filter screen 11 can be used stainless steel, also high pressure resistant disinfectant plastics of available energy such as polypropylene, compactings such as polytetrafluoroethylene; Overcoat 2 is made up of loam cake 21, sleeve 22 and drain pipe 23, the material of overcoat 2 adopts stainless steel to make, overcoat 2 diameters are during with one day two volume of continuous drawing liquid, microcarrier 3 is not drawn out of and is the best, overcoat 2 is long being suitable from about the top of retort 4 and each 3cm of bottom, half that inner prop 1 is about to overcoat, diameter less than overcoat 10mm about.Two drain pipes 13,23 place the upper end of inner prop 1 and overcoat 2 respectively, and its diameter and length can design according to the interface of the top cover of retort 4, so that link to each other with the drawing liquid pipeline, the two-in-one perfusion post of filtering-precipitation places in the retort 4.Peristaltic pump is a peristaltic pump can long-term operation, forward and reverse rotation, comprises drawing liquid peristaltic pump 5 and reinforced peristaltic pump 6, and drawing liquid peristaltic pump 5 is connected with drawing liquid jar 7, and reinforced peristaltic pump 6 is connected with feed pot 8.Two-position controller 9 is can be directed and the electronic digit two-position controller of timing control peristaltic pump sense of rotation and time, as the disclosed two-position controller of ZL91208893.1 etc.
Concrete utilization example: cultivate CHO engineering cell A2 with 5L retort 4 and produce a kind of genetically engineered thrombolytic drug TNK-tPA, adopted microcarrier serum-free perfusion to cultivate.Microcarrier 3 adopts the Cytopore porous microcarrier, working concentration is 1mg/ml, adjust the rotating time length of drawing liquid peristaltic pump 5 in the cultivation with two-position controller 9, by inner prop 1 drawing liquid and the liquor charging of the two-in-one perfusion post of filtering-precipitation, make perfusion flow (promptly entering the amount of drawing liquid jar 7) increase to 1 of every day gradually more than the volume.Every morning, with the about 200ml of fresh culture in the feed pot 8, the short period of time was by the Kai Heguan of three-way pipeline 10 and a plurality of pipeline folder 11, flushing filter screen with reinforced peristaltic pump 6.So cultured continuously is 49 days, harvested cell supernatant 269L altogether, and the TNK-tPA activity is up to 19440IU/ml, and the high-density of cell reaches 5 * 10
6/ ml, perfusion pillar cell rejection reaches 92.3%, and experiment finishes the back to be checked, does not see that filter screen has any obstruction, has obtained good result, as shown in Figure 2.
In a word, the present invention has exemplified above-mentioned preferred implementation, but should illustrate, obviously those skilled in the art can carry out various variations and remodeling.Therefore, unless such variation and remodeling have departed from scope of the present invention, otherwise all should be included in protection scope of the present invention.
Claims (4)
1. one kind is used for the filtering-precipitation double combination perfusion system that the zooblast perfusion is cultivated, it is characterized in that: comprise the two-in-one perfusion post of filtering-precipitation, peristaltic pump, two-position controller, three-way pipeline and pipeline folder, the two-in-one perfusion post of described filtering-precipitation is connected with peristaltic pump by three-way pipeline, and peristaltic pump is by two-position controller control running, and the pipeline folder is arranged on the pipeline that is connected between the two-in-one perfusion post of filtering-precipitation and the peristaltic pump; The two-in-one perfusion post of described filtering-precipitation comprises inner prop and overcoat, inner prop is enclosed within the overcoat, wherein inner prop is made up of filter screen, support and the drain pipe in 20～70 μ m apertures, overcoat is made up of loam cake, sleeve and drain pipe, the drain pipe of inner prop places the upper end of inner prop and overcoat respectively with the drain pipe of overcoat and links to each other with the drawing liquid pipeline, and the two-in-one perfusion post of filtering-precipitation places in the retort.
2. the filtering-precipitation double combination perfusion system that is used for the cultivation of zooblast perfusion according to claim 1, it is characterized in that: described peristaltic pump is a peristaltic pump can long-term operation, forward and reverse rotation, comprise drawing liquid peristaltic pump and reinforced peristaltic pump, the drawing liquid peristaltic pump is connected with the drawing liquid jar, and reinforced peristaltic pump is connected with feed pot.
3. the filtering-precipitation double combination perfusion system that is used for the cultivation of zooblast perfusion according to claim 1 and 2 is characterized in that: described two-position controller is can orientation and the electronic digit two-position controller of timing control peristaltic pump sense of rotation and time.
4. the filtering-precipitation double combination perfusion system that is used for the cultivation of zooblast perfusion according to claim 3, it is characterized in that: the inner prop of the two-in-one perfusion post of described filtering-precipitation is connected in feed pot by three-way pipeline, and is used for the filter screen of Artificial Control fresh culture flushing inner prop by pipeline folder trip switch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100288536A CN101085978B (en) | 2007-06-27 | 2007-06-27 | Filtering-precipitation dual perfusion system for animal cell perfusion culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100288536A CN101085978B (en) | 2007-06-27 | 2007-06-27 | Filtering-precipitation dual perfusion system for animal cell perfusion culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101085978A CN101085978A (en) | 2007-12-12 |
| CN101085978B true CN101085978B (en) | 2011-01-05 |
Family
ID=38937137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100288536A Active CN101085978B (en) | 2007-06-27 | 2007-06-27 | Filtering-precipitation dual perfusion system for animal cell perfusion culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101085978B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102690781A (en) * | 2012-04-28 | 2012-09-26 | 苏州金盟生物技术有限公司 | Method for replacing culture medium in perfusion-type cell culture |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103031250B (en) * | 2012-10-17 | 2015-06-17 | 浙江赛尚医药科技有限公司 | Cell culture pipeline device and automatic cell culture system |
| SG11201806310TA (en) * | 2016-01-26 | 2018-08-30 | Boehringer Ingelheim Int | Linked perfusion to continuous-flow stirred-tank reactor cell culture system |
| CN105820952A (en) * | 2016-03-31 | 2016-08-03 | 上海拜高乐生物技术有限公司 | Bioreactor for cell perfusion culture |
| CN108865885B (en) * | 2018-07-05 | 2021-03-16 | 广州铭康生物工程有限公司 | Cell culture interception device and cell culture interception method |
| WO2024056037A1 (en) * | 2022-09-14 | 2024-03-21 | 北京华龛生物科技有限公司 | Liquid drawing device, bioreactor, and liquid drawing method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990011345A1 (en) * | 1989-03-17 | 1990-10-04 | Baxter International Inc. | Spin filter for removing substantially cell-free culture medium from suspension cell culture system |
| EP0909811A2 (en) * | 1997-10-16 | 1999-04-21 | B. BRAUN CAREX S.p.A. | Bioreactor for culturing animal and/or human cells, particularly hepatocytes |
| CN2420286Y (en) * | 2000-03-20 | 2001-02-21 | 上海中路生物工程有限公司 | New organized irrigation device |
-
2007
- 2007-06-27 CN CN2007100288536A patent/CN101085978B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990011345A1 (en) * | 1989-03-17 | 1990-10-04 | Baxter International Inc. | Spin filter for removing substantially cell-free culture medium from suspension cell culture system |
| EP0909811A2 (en) * | 1997-10-16 | 1999-04-21 | B. BRAUN CAREX S.p.A. | Bioreactor for culturing animal and/or human cells, particularly hepatocytes |
| CN2420286Y (en) * | 2000-03-20 | 2001-02-21 | 上海中路生物工程有限公司 | New organized irrigation device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102690781A (en) * | 2012-04-28 | 2012-09-26 | 苏州金盟生物技术有限公司 | Method for replacing culture medium in perfusion-type cell culture |
| CN102690781B (en) * | 2012-04-28 | 2014-04-30 | 苏州金盟生物技术有限公司 | Method for replacing culture medium in perfusion-type cell culture |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101085978A (en) | 2007-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101085978B (en) | Filtering-precipitation dual perfusion system for animal cell perfusion culture | |
| CN101519634B (en) | Open type biological reactor for microalgae cultivation and microalgae cultivation method | |
| CN101897308B (en) | Equipment and method for collecting industrially cultivated pelagic eggs | |
| CN208807387U (en) | A kind of photovoltaic fish and vegetable symbiotic system | |
| CN105724304A (en) | Layered fish and shrimp circulating water polyculture system | |
| CN206034403U (en) | Automatic clean -up equipment of pond water surface garbage breeds | |
| CN102531293A (en) | Centralized treatment system for sewage in rural area | |
| CN112980649A (en) | Hollow tube bundle type fixed bed bioreactor for adherent culture of breast-feeding cells | |
| CN101692782B (en) | Fertigation facility of domestic sewage after biochemical treatment | |
| CN109220965B (en) | Circulating water culture container and culture system | |
| CN210438740U (en) | Microalgae in-situ expanding culture system for shellfish culture pond | |
| CN207885494U (en) | A kind of cultivating box for leech convenient for batch cultivation | |
| CN203027914U (en) | Net cage type culture pond for experiment | |
| CN206024898U (en) | Automatic clean recirculating aquaculture pond of industrialization | |
| CN108410715A (en) | A kind of lignocellulose raw material high solid methane phase system and its process | |
| CN208649316U (en) | A kind of lignocellulose raw material high solid methane phase system | |
| CN202019628U (en) | Aquaculture system | |
| CN102268375B (en) | Method and system for continuously collecting algae liquid cultured from unicellular algae | |
| CN103130381B (en) | VUN treatment construction method for domestic sewage | |
| CN211210738U (en) | Agricultural ecological system | |
| CN218244642U (en) | Novel family farm energy cyclic utilization device | |
| CN204634769U (en) | A kind of multifunctional culture pond | |
| CN108990873A (en) | From irrigation formula rice field fish culture system | |
| CN107173210A (en) | The cultivator and its cultural method of a kind of long-term cropping | |
| CN208768749U (en) | A kind of stereo biological cultivation equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 510000 No.1, jinfengyuan Road, Guangzhou Economic and Technological Development Zone, Guangdong Province Patentee after: Shijiazhuang Pharmaceutical Group mingfule Pharmaceutical (Guangzhou) Co.,Ltd. Address before: 510730 Friendship Road 173, Guangzhou economic and Technological Development Zone, Guangdong, China Patentee before: GUANGZHOU RECOMGEN BIOTECH CO.,LTD. |
|
| CP03 | Change of name, title or address |