CN2420286Y - New organized irrigation device - Google Patents
New organized irrigation device Download PDFInfo
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- CN2420286Y CN2420286Y CN 00216922 CN00216922U CN2420286Y CN 2420286 Y CN2420286 Y CN 2420286Y CN 00216922 CN00216922 CN 00216922 CN 00216922 U CN00216922 U CN 00216922U CN 2420286 Y CN2420286 Y CN 2420286Y
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- perfusion
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Abstract
The utility model relates to a new organized irrigation device, which can irrigate samples under a separating condition, such as tissues, cultured cells, organs, artificial biomembranes, etc. and can collect irrigation liquid to detect the biological substance or/ and activity, or/and to directly detect the change and/or the biochemical reaction of the biologically active substance of the irritation substance to establish a pharmaceutical research model on the foundation. The utility model has the advantages of high obvious irritation stability and repeatability.
Description
The present invention relates to a kind of new perfusion device, this device can carry out perfusion to samples such as tissue, culturing cell, organ and artificial membranes under isolated condition, collect perfusate and carry out biological substance or/and determination of activity, or/and directly measure the variation and/or the biochemical reaction of the biologically active substance of perfusion material, and can set up the study of pharmacy model based on this.
The perfusion of isolated condition undertissue, culturing cell, organ and artificial membrane, because the problem such as stable of the adhering to of floating, the cell of temperature controlling, tissue slice, organ, directly influence is organized in the survival rate in the perfusing course and the loss of perfusion sample, thereby it is very big to make that experiment condition is difficult to the fluctuation of unification, experimental result, the repeatability of experiment, harmoniously is difficult to control.These all cause the research work of in vitro tissue or organ level to be subjected to very big influence, therefore how to improve the stable and repeated of perfusion, are the problems that this area presses for solution always.
Purpose of the present invention is exactly to improve a kind of new tissue perfusion device, by improving the state of sample when the perfusion, reaches stable experiment condition, thereby improves experiment and/or test repeatability.
Goal of the invention of the present invention is achieved by the following technical solution: a kind of perfused tissue device, and it comprises:
One entrance end member (1), it has one and introduces mouth portion (11), and a neck (12) and one first interface section (13) are filtering and holding device (14) near being provided with first on the position of first interface section of this member;
One exit end member (2), it has ejection nozzle part (21), and a neck (22) and one second interface section (23) are filtering and holding device (24) near being provided with first on the position of first interface section of this member;
One perfusion groove body (3), the 3rd interface section (32) and the 4th interface section (33) that it has a perfusion main body (31) and can combine closely with first and second interface sections respectively, described perfusion main body is made of inside and outside two side, and be provided with the opening (34) that communicates with the formed space of inside and outside wall, so that introduce thermostatic medium.
Preferably, handle through frosted the described first, second, third and the 4th interface section.
Preferably, described first and second interface sections, and third and fourth interface section is the mutual taper shape of coupling, to realize sealing.
In the accompanying drawings,
Fig. 1 has shown a kind of perfusion device of the present invention;
Fig. 2 A has shown a kind of structure of first interface section of entrance end member;
Fig. 2 B has shown a kind of structure of second interface section of exit end member;
Fig. 3 has shown that the another kind of first interface section of entrance end member changes structure;
Fig. 4 has shown that the another kind of first interface section of entrance end member changes structure;
Fig. 5 has shown that the another kind of first interface section of entrance end member changes structure;
Fig. 6 has shown a kind of structure that is located at the port connecting apparatus between the perfusion groove body;
Fig. 7 has shown the another kind of structure that is located at the port connecting apparatus between the perfusion groove body;
Fig. 8 has shown a kind of syndeton that is used for the perfusate biological activity determination;
Fig. 9 has shown the another kind of syndeton that is used for the perfusate biological activity determination;
Figure 10 has shown the another kind of syndeton that is used for the perfusate biological activity determination.
In perfusing course, the stability of control perfusion sample in the perfusion pond is to improve whole tissue perfusion The key of experiment. Adopt new perfusion device of the present invention, can control fine and easily the perfusion sample The stability of product in the perfusion pond, thus experiment condition stablized, improved experiment and/or test repeatability.
Now referring to Fig. 1, shown an embodiment of perfusion device of the present invention among the figure. This device is divided into three ones Divide, lower, in, upper three parts are respectively perfusate entrance point member (1), perfusion groove body (3), perfusate The port of export (2).
In perfusion device of the present invention, the below is for the entrance point member (1) of introducing perfusate, It has one and introduces mouth portion (11), and a neck (12) and first interface part (13) are at this The position of the close first interface part of member is provided with first and filters and holding device (14). Entrance point The caliber size of the water inlet of member (1) (namely introducing mouth portion (11)) can change, and is various through permanent to admit The perfusate of temperatureization. Its upper end delivery port skin of entrance point member (1) is preferably ground, so as to combine closely in The lower end of perfusion groove body (i.e. the 3rd interface section (32)) and not making leaks. Can in entrance point member (1) inside The porose porcelain sieve of sealing-in can carry histotomy and can pass through perfusate again on the porcelain sieve, this part is by different purposes Can do remodeling, for example make the structure shown in Fig. 2 A, 3,4,5, thus be respectively applied to cultured cell, The perfusions such as organ.
In perfusion device of the present invention, the top be for the port of export member (2) of deriving perfusate, It has ejection nozzle part (21), and a neck (22) and one second interface section (23) are at this structure The position of the close first interface part of part is provided with first and filters and holding device (24). Port of export structure The skin of second inlet part (23) of part is preferably ground, thereby combines closely in the upper end of perfusion groove body (3) (i.e. the 4th interface section (33)) and do not make and leak. But at the porose porcelain sieve of the inside of port of export member sealing-in. Porcelain Sieve can allow to pass through perfusate. This part also can be done remodeling by different purposes, for example makes shown in Fig. 6,7 Structure. For example first interface is partly gone up and can be had groove, to insert slide. Perhaps at first interface Cover has screen cloth (silk screen) on the part.
In perfusion device of the present invention, in the middle of being positioned at is perfusion groove body (3), and it has a perfusion Main body (31) and the 3rd interface section (32) that can combine closely with first and second interface sections respectively With the 4th interface section (33), described perfusion main body is made of inside and outside two side, and is provided with and inside and outside wall The opening that formed space communicates (34) is in order to introduce thermostatic medium. Usually, the perfusion groove master at middle part Body is the jacket layer of layer glass, adopts thermostatic medium (for example water) loop control temperature, and two ends (namely about it The the 4th and the 3rd interface section (33 and 32)) is preferably ground. As for perfusion groove internal diameter, be not particularly limited, Can make different big ting models according to the perfusion sample.
Sometimes, for 2 of perfusions or a plurality of sample and observe correlation between them, may need Perfusion device with a plurality of perfusion groove bodies. This moment, apparatus of the present invention can comprise 2-5 perfusion groove usually Main body, and between the perfusion groove body ports having jockey (Fig. 6,7).
In apparatus of the present invention, first and second interface sections, and the knot of third and fourth interface section Structure is mutually to mate, and for example is the taper shape that mutually mates, to realize sealing.
The available usually material that is selected from down group of apparatus of the present invention is made: glass, synthetic glass, engineering plastics and other biological organize acceptable material to make.
Perfusion device of the present invention has been widely used, and for example is used for perfusions such as the section of isolated condition undertissue, culturing cell, organ and artificial membrane, and integrates the set of the device of other biological determination of activity.Apparatus of the present invention also can be used for measuring the existence of biologically active substance in the perfusate and/or the activity of biologically active substance, or/and biological activity wherein such as perfusion tissue, cell, organ, artificial rust changes or/and biochemical reaction in the determinator.Apparatus of the present invention are particularly useful for study of pharmacy.
For example apparatus of the present invention can be used for various cells, tissue and organ.Other tissue slicies such as cerebral tissue, liver, kidney for example.The source of these samples can be from various organisms, comprising (but being not limited to): rat, pig, ox, sheep etc.These samples can be can be natural or artificial culture.
The preparation of embodiment 1, perfusion device
Make perfusion device shown in Figure 1 with glass, wherein the internal diameter of perfusion groove body is the 5-20 millimeter, highly is the 20-100 millimeter.The size of entrance end member and exit end member and perfusion groove body are complementary.
Embodiment 2, rat brain sheet perfusion experiment
Adopt neonate rat, separate pallium, be prepared into the thick brain sheet of 400um, hatch in tissue buffer solution.Adopt the tissue perfusion device of making among the embodiment 1 as shown in Figure 1, volume according to the perfusion sample, select entrance end and exit end (as shown in Figure 2), make that the size of perfusion groove is that the 2-4 of perfusion sample volume is doubly big, earlier will top exit end and after the perfusion groove body fixes, device is turned around, seal water outlet, add perfusate and tissue, cover water-in, make perfusate flow through the perfusion groove, more whole device is turned around from water-in, constant temperature, flow velocity and the logical oxygen of control perfusate, balance can begin experiment after 90 minutes.
The activity that serum lactic dehydrogenase in the perfusate can be directly measured in experiment changes with cells injury situation in the calibrating brain sheet.
Experiment also can be measured the variation of a certain biochemical substances content in the brain sheet, as the release of neurotransmitter acetylcholine, as the device combination of Fig. 8, adopts biologic assay directly to measure the dynamic change that vagusstoff discharges.
Experiment also can be measured the release of two kinds of biologically active substances in the brain sheet simultaneously or/and active variation, as the device combination of Fig. 9.
Experiment also can adopt 96 orifice plates to collect that perfusate is used for enzyme-linked immunoassay or/and other biochemical measurements, as the device combination of Figure 10.
Experiment also can be taken out the brain sheet behind perfusion, after fixing with stationary liquid, is used for histologic analysis.
This method can be used for other tissue slicies such as liver, kidney equally except that can be used for brain tissue slice, the source of such tissue slice also not only is confined to rat, also can be applicable to other laboratory animal, comprises animals such as pig, ox.
The perfusion experiment of embodiment 3, culturing cell
Slide glass is cut into the rectangular of required width, after the cleaning, is laid in 6 well culture plates, make cell be grown on the slide glass, after treating that cell covers with slide glass, slide glass is inserted in the groove of perfusate entrance end porcelain sieve as shown in Figure 3, slide glass is put into the main body of perfusion groove and carried out perfusion.
Experiment also can be adopted device as shown in Figure 4, and cell is grown on the small-sized plastic mesh, and this screen cloth is mounted on the perfusate entrance end porcelain sieve.
Experiment also can be adopted screen cloth as shown in Figure 4, artificial membrane is grown on such plastic mesh be used for perfusion.
The mensuration project of experiment can adopt embodiment 2 to carry out like that.
The perfusion experiment of embodiment 4, tissue
The acute isolation induced lung is prepared into the 600um slab, hatches in tissue buffer solution.Adopt tissue perfusion device,, select entrance end and exit end (as shown in Figure 5) according to the volume of perfusion sample as Fig. 1, make that tissue sample can be open and flat in the perfusion groove, form the perfusion cross section of similar barrier,, can begin experiment after the balance as embodiment 2 operations.
This method is except that can be used for lung sections, can be used for other tissue slicies such as liver, kidney equally, or/and from the organs such as blood vessel isolating tissue film, the source of such sample also not only is confined to rat, also can be applicable to other laboratory animal, comprise animals such as pig, ox.
But the mensuration project similar embodiment 2 of experiment is carried out like that.
The perfusion experiment of embodiment 5, tissue
The two or more samples of perfusion are also observed mutual relationship therebetween simultaneously, can adopt port connecting apparatus as Fig. 6, make that several perfusion grooves are placed in-line, but the perfusate Continuous Flow is through a plurality of perfusion samples, as measuring the variation of middle-chain, can adopt the placed in-line port connecting apparatus that has the edge can supply the sampling opening as shown in Figure 7.
But the mensuration project similar embodiment 2 of experiment is carried out like that.
Although the present invention is described in conjunction with most preferred embodiment, clearly those skilled in the art can carry out various changes to the present invention after having read the present invention.These are changed within the scope of the invention fall within claim equally and limited.
Claims (7)
1. tissue perfusion device is characterized in that it comprises:
One entrance end member (1), it has one and introduces mouth portion (11), and a neck (12) and one first interface section (13) are filtering and holding device (14) near being provided with first on the position of first interface section of this member;
One exit end member (2), it has ejection nozzle part (21), and a neck (22) and one second interface section (23) are filtering and holding device (24) near being provided with first on the position of first interface section of this member;
Perfusion groove body (3), the 3rd interface section (32) and the 4th interface section (33) that it has a perfusion main body (31) and can combine closely with first and second interface sections respectively, described perfusion main body is made of inside and outside two side, and be provided with the opening (34) that communicates with the formed space of inside and outside wall, so that introduce thermostatic medium.
2. device as claimed in claim 1 is characterized in that, handle through frosted the described first, second, third and the 4th interface section.
3. device as claimed in claim 1 is characterized in that, described first and second interface sections, and third and fourth interface section is the mutual taper shape of coupling, to realize sealing.
4. device as claimed in claim 1 is characterized in that, this device is to make with the material that is selected from down group: glass, synthetic glass, engineering plastics.
5. device as claimed in claim 1 is characterized in that, and is fluted on described first interface section, to insert slide glass.
6. device as claimed in claim 1 is characterized in that, is with screen cloth on described first interface section.
7. device as claimed in claim 1 is characterized in that, this device comprises 2-5 perfusion groove body, and between the perfusion groove body ports having coupling device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00216922 CN2420286Y (en) | 2000-03-20 | 2000-03-20 | New organized irrigation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00216922 CN2420286Y (en) | 2000-03-20 | 2000-03-20 | New organized irrigation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2420286Y true CN2420286Y (en) | 2001-02-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00216922 Expired - Fee Related CN2420286Y (en) | 2000-03-20 | 2000-03-20 | New organized irrigation device |
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| CN (1) | CN2420286Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332719C (en) * | 2003-06-24 | 2007-08-22 | 中国人民解放军第三军医大学 | Simple extraneous liver perfusion device for digesting and separating liver cells |
| CN101085978B (en) * | 2007-06-27 | 2011-01-05 | 广州铭康生物工程有限公司 | Filtering-precipitation dual perfusion system for animal cell perfusion culture |
| CN102813559A (en) * | 2012-08-27 | 2012-12-12 | 山东大学 | Full-automatic hemoperfusion device |
| CN103018430A (en) * | 2012-11-22 | 2013-04-03 | 上海交通大学医学院附属仁济医院 | Serially-connected blood vessel grooves for blood vessel perfusion system |
| CN104713907A (en) * | 2015-01-29 | 2015-06-17 | 新乡医学院 | In vivo brain slice nerve network oscillation recording system and recording method thereof |
-
2000
- 2000-03-20 CN CN 00216922 patent/CN2420286Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332719C (en) * | 2003-06-24 | 2007-08-22 | 中国人民解放军第三军医大学 | Simple extraneous liver perfusion device for digesting and separating liver cells |
| CN101085978B (en) * | 2007-06-27 | 2011-01-05 | 广州铭康生物工程有限公司 | Filtering-precipitation dual perfusion system for animal cell perfusion culture |
| CN102813559A (en) * | 2012-08-27 | 2012-12-12 | 山东大学 | Full-automatic hemoperfusion device |
| CN103018430A (en) * | 2012-11-22 | 2013-04-03 | 上海交通大学医学院附属仁济医院 | Serially-connected blood vessel grooves for blood vessel perfusion system |
| CN104713907A (en) * | 2015-01-29 | 2015-06-17 | 新乡医学院 | In vivo brain slice nerve network oscillation recording system and recording method thereof |
| CN104713907B (en) * | 2015-01-29 | 2017-09-22 | 新乡医学院 | Brain slice in vitro neutral net vibrates record system and the method for record |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: SHANGHAI GENON BIOENGINEERING CO., LTD. Free format text: FORMER NAME OR ADDRESS: ZHONGLU BIO-ENGINEERING CO LTD, SHANGHAI |
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| CP03 | Change of name, title or address |
Address after: 201203 Shanghai Zhangjiang Cailun Road No. 88 Patentee after: Jielong Bioengineering Co., Ltd., Shanghai Address before: 201300 Shanghai city Nanhui Sanzao Industrial Park No. 999 Patentee before: Zhonglu Biological Engineering Co., Ltd., Shanghai |
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| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |