CN101084233B - 用于在糖蛋白产生中减少或消除α-甘露糖苷酶抗性聚糖的方法 - Google Patents

用于在糖蛋白产生中减少或消除α-甘露糖苷酶抗性聚糖的方法 Download PDF

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Publication number
CN101084233B
CN101084233B CN2005800137925A CN200580013792A CN101084233B CN 101084233 B CN101084233 B CN 101084233B CN 2005800137925 A CN2005800137925 A CN 2005800137925A CN 200580013792 A CN200580013792 A CN 200580013792A CN 101084233 B CN101084233 B CN 101084233B
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pichia
host cell
nucleic acid
mannose
mannosidase
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CN101084233A (zh
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P·波布罗维茨
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Glycofi Inc
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Glycofi Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN2005800137925A 2004-04-29 2005-04-29 用于在糖蛋白产生中减少或消除α-甘露糖苷酶抗性聚糖的方法 Expired - Lifetime CN101084233B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US56673604P 2004-04-29 2004-04-29
US62018604P 2004-10-18 2004-10-18
US60/620,186 2004-10-18
PCT/IB2005/051415 WO2005106010A2 (en) 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins

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CN101084233A CN101084233A (zh) 2007-12-05
CN101084233B true CN101084233B (zh) 2012-08-15

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US (3) US7465577B2 (enExample)
EP (1) EP1747280B1 (enExample)
JP (1) JP4954866B2 (enExample)
CN (1) CN101084233B (enExample)
AU (1) AU2005238308B8 (enExample)
CA (1) CA2565125A1 (enExample)
WO (1) WO2005106010A2 (enExample)

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US20060024304A1 (en) * 2000-06-28 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Man5GlcNAc2 glycoform
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
EP2359685B1 (en) * 2001-12-27 2013-12-04 GlycoFi, Inc. Methods to engineer mammalian-type carbohydrate structures
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
US6964784B2 (en) * 2002-03-07 2005-11-15 Optigenex, Inc. Method of preparation and composition of a water soluble extract of the bioactive component of the plant species uncaria for enhancing immune, anti-inflammatory, anti-tumor and dna repair processes of warm blooded animals
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US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
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US9598682B2 (en) 2009-09-29 2017-03-21 Vib Vzw Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
CN102648286A (zh) * 2009-10-16 2012-08-22 默沙东公司 在巴斯德毕赤氏酵母中生产缺乏与针对宿主细胞抗原的抗体的可检测交叉结合活性的蛋白质的方法
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US8158384B2 (en) 2012-04-17
US20090117616A1 (en) 2009-05-07
US7465577B2 (en) 2008-12-16
JP4954866B2 (ja) 2012-06-20
EP1747280A4 (en) 2011-11-09
AU2005238308B8 (en) 2009-10-08
US7713719B2 (en) 2010-05-11
CN101084233A (zh) 2007-12-05
WO2005106010A2 (en) 2005-11-10
US20060211085A1 (en) 2006-09-21
EP1747280A2 (en) 2007-01-31
AU2005238308A1 (en) 2005-11-10
EP1747280B1 (en) 2018-01-17
WO2005106010A3 (en) 2007-08-16
JP2007534330A (ja) 2007-11-29
AU2005238308B2 (en) 2009-05-14
US20090093020A1 (en) 2009-04-09
CA2565125A1 (en) 2005-11-10

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