AU2005238308B8 - Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins - Google Patents

Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins Download PDF

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Publication number
AU2005238308B8
AU2005238308B8 AU2005238308A AU2005238308A AU2005238308B8 AU 2005238308 B8 AU2005238308 B8 AU 2005238308B8 AU 2005238308 A AU2005238308 A AU 2005238308A AU 2005238308 A AU2005238308 A AU 2005238308A AU 2005238308 B8 AU2005238308 B8 AU 2005238308B8
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Prior art keywords
seq
nucleic acid
host cell
polypeptide
pichia
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Ceased
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AU2005238308A
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AU2005238308A1 (en
AU2005238308B2 (en
Inventor
Piotr Bobrowicz
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Glycofi Inc
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Glycofi Inc
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Publication of AU2005238308B2 publication Critical patent/AU2005238308B2/en
Publication of AU2005238308B8 publication Critical patent/AU2005238308B8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AU2005238308A 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins Ceased AU2005238308B8 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US56673604P 2004-04-29 2004-04-29
US62018604P 2004-10-18 2004-10-18
US60/620,186 2004-10-18
PCT/IB2005/051415 WO2005106010A2 (en) 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins

Publications (3)

Publication Number Publication Date
AU2005238308A1 AU2005238308A1 (en) 2005-11-10
AU2005238308B2 AU2005238308B2 (en) 2009-05-14
AU2005238308B8 true AU2005238308B8 (en) 2009-10-08

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Family Applications (1)

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AU2005238308A Ceased AU2005238308B8 (en) 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins

Country Status (7)

Country Link
US (3) US7465577B2 (enExample)
EP (1) EP1747280B1 (enExample)
JP (1) JP4954866B2 (enExample)
CN (1) CN101084233B (enExample)
AU (1) AU2005238308B8 (enExample)
CA (1) CA2565125A1 (enExample)
WO (1) WO2005106010A2 (enExample)

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US20060029604A1 (en) * 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US7795002B2 (en) * 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US20060034828A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform
ATE440959T1 (de) * 2000-06-28 2009-09-15 Glycofi Inc Verfahren für die herstellung modifizierter glykoproteine
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US20060024304A1 (en) * 2000-06-28 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Man5GlcNAc2 glycoform
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
EP2359685B1 (en) * 2001-12-27 2013-12-04 GlycoFi, Inc. Methods to engineer mammalian-type carbohydrate structures
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
US6964784B2 (en) * 2002-03-07 2005-11-15 Optigenex, Inc. Method of preparation and composition of a water soluble extract of the bioactive component of the plant species uncaria for enhancing immune, anti-inflammatory, anti-tumor and dna repair processes of warm blooded animals
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WO2005090552A2 (en) * 2004-03-17 2005-09-29 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
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EP1937305A4 (en) * 2005-09-09 2008-10-08 Glycofi Inc IMMUNOGLOBULIN CONTAINING PRESENTLY A MAN7GLCNAC2, MAN8GLCNAC2 GLYCOFORM
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US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
DK2240595T4 (da) * 2008-01-03 2019-10-07 Cornell Res Foundation Inc Glykosyleret proteinekspression i prokaryoter
JP2011514157A (ja) * 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
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AU2009251328B2 (en) * 2008-05-30 2014-10-02 Glycofi, Inc. Yeast strain for the production of proteins with terminal alpha-1,3-linked galactose
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US9528116B2 (en) 2009-10-30 2016-12-27 Merck Sharp & Dohme Corp. Method for producing therapeutic proteins in Pichia pastoris lacking dipeptidyl aminopeptidase activity
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KR101930961B1 (ko) 2010-02-24 2018-12-19 머크 샤프 앤드 돔 코포레이션 피키아 파스토리스에서 생산된 치료 당단백질 상의 ｎ-글리코실화 부위 점유를 증가시키는 방법
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US9416173B2 (en) * 2011-04-21 2016-08-16 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Peptide and conjugate vaccines for fungal infections
JP2014517846A (ja) 2011-05-25 2014-07-24 メルク・シャープ・アンド・ドーム・コーポレーション 改善された特性を有するＦｃ含有ポリペプチドの製造方法
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US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
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EP1283265A1 (en) * 2000-05-17 2003-02-12 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins

Also Published As

Publication number Publication date
US8158384B2 (en) 2012-04-17
US20090117616A1 (en) 2009-05-07
US7465577B2 (en) 2008-12-16
JP4954866B2 (ja) 2012-06-20
CN101084233B (zh) 2012-08-15
EP1747280A4 (en) 2011-11-09
US7713719B2 (en) 2010-05-11
CN101084233A (zh) 2007-12-05
WO2005106010A2 (en) 2005-11-10
US20060211085A1 (en) 2006-09-21
EP1747280A2 (en) 2007-01-31
AU2005238308A1 (en) 2005-11-10
EP1747280B1 (en) 2018-01-17
WO2005106010A3 (en) 2007-08-16
JP2007534330A (ja) 2007-11-29
AU2005238308B2 (en) 2009-05-14
US20090093020A1 (en) 2009-04-09
CA2565125A1 (en) 2005-11-10

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