CA2565125A1 - Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins - Google Patents

Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins Download PDF

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Publication number
CA2565125A1
CA2565125A1 CA002565125A CA2565125A CA2565125A1 CA 2565125 A1 CA2565125 A1 CA 2565125A1 CA 002565125 A CA002565125 A CA 002565125A CA 2565125 A CA2565125 A CA 2565125A CA 2565125 A1 CA2565125 A1 CA 2565125A1
Authority
CA
Canada
Prior art keywords
seq
nucleic acid
acid sequence
pichia
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002565125A
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English (en)
French (fr)
Inventor
Piotr Bobrowicz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glycofi Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2565125A1 publication Critical patent/CA2565125A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002565125A 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins Abandoned CA2565125A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US56673604P 2004-04-29 2004-04-29
US62018604P 2004-10-18 2004-10-18
US60/620,186 2004-10-18
PCT/IB2005/051415 WO2005106010A2 (en) 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins

Publications (1)

Publication Number Publication Date
CA2565125A1 true CA2565125A1 (en) 2005-11-10

Family

ID=40588462

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002565125A Abandoned CA2565125A1 (en) 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins

Country Status (7)

Country Link
US (3) US7465577B2 (enExample)
EP (1) EP1747280B1 (enExample)
JP (1) JP4954866B2 (enExample)
CN (1) CN101084233B (enExample)
AU (1) AU2005238308B8 (enExample)
CA (1) CA2565125A1 (enExample)
WO (1) WO2005106010A2 (enExample)

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US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
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US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
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EP1937305A4 (en) * 2005-09-09 2008-10-08 Glycofi Inc IMMUNOGLOBULIN CONTAINING PRESENTLY A MAN7GLCNAC2, MAN8GLCNAC2 GLYCOFORM
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BRPI0711898A2 (pt) * 2006-05-19 2011-12-27 Glycofi Inc composiÇço de proteÍna da eritropoietina substancialmente homogÊnea, composiÇço farmacÊutica, e, mÉtodos de aumentar hematàcrito em um mamÍfero, e de produzir uma composiÇço de eritropoietina
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EP2263089B1 (en) * 2008-03-03 2015-01-28 GlycoFi, Inc. Surface display of recombinant proteins in lower eukaryotes
AU2009251328B2 (en) * 2008-05-30 2014-10-02 Glycofi, Inc. Yeast strain for the production of proteins with terminal alpha-1,3-linked galactose
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US9598682B2 (en) 2009-09-29 2017-03-21 Vib Vzw Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
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AU2010313608A1 (en) 2009-10-30 2012-06-07 Merck Sharp & Dohme Corp. Methods for the production of recombinant proteins with improved secretion efficiencies
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JP2013511272A (ja) 2009-11-19 2013-04-04 オキシレイン ユーケー リミテッド 哺乳類様複合ｎ−グリカンを生成する酵母株
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US9416173B2 (en) * 2011-04-21 2016-08-16 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Peptide and conjugate vaccines for fungal infections
JP2014517846A (ja) 2011-05-25 2014-07-24 メルク・シャープ・アンド・ドーム・コーポレーション 改善された特性を有するＦｃ含有ポリペプチドの製造方法
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Also Published As

Publication number Publication date
US8158384B2 (en) 2012-04-17
US20090117616A1 (en) 2009-05-07
US7465577B2 (en) 2008-12-16
JP4954866B2 (ja) 2012-06-20
CN101084233B (zh) 2012-08-15
EP1747280A4 (en) 2011-11-09
AU2005238308B8 (en) 2009-10-08
US7713719B2 (en) 2010-05-11
CN101084233A (zh) 2007-12-05
WO2005106010A2 (en) 2005-11-10
US20060211085A1 (en) 2006-09-21
EP1747280A2 (en) 2007-01-31
AU2005238308A1 (en) 2005-11-10
EP1747280B1 (en) 2018-01-17
WO2005106010A3 (en) 2007-08-16
JP2007534330A (ja) 2007-11-29
AU2005238308B2 (en) 2009-05-14
US20090093020A1 (en) 2009-04-09

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Effective date: 20170116