CN101077045A - Artificial raise seedling method for red bilberry - Google Patents
Artificial raise seedling method for red bilberry Download PDFInfo
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- CN101077045A CN101077045A CN 200710065992 CN200710065992A CN101077045A CN 101077045 A CN101077045 A CN 101077045A CN 200710065992 CN200710065992 CN 200710065992 CN 200710065992 A CN200710065992 A CN 200710065992A CN 101077045 A CN101077045 A CN 101077045A
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- blueberry
- seedling method
- raise seedling
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Abstract
The present invention provides artificial blueberry seedling raising process, and features that the blueberry seed after being sterilized is inoculated to MS culture medium under aseptic condition for culturing under tissue culture condition. The present invention meets the specific requirement of blueberry seed during germination, and has simple operation, short seedling raising period, high germination rate and other advantages. The present invention makes it possible to obtain great amount of blueberry seedlings in short term by means of industrial production.
Description
Technical field
The present invention relates to a kind of artificial raise seedling method for red bilberry.
Background technology
Blueberry (blueberry) is called blue berry, blueberry, the blue certain kind of berries again, is Ericaceae (Ericaceae) genus vaccinium (Vaccinium L.) plant.It is blue that the blueberry fruit is, and on average weighs 0.5～2.5g, maximum 5g, and fruit colour beauty, pleasing, the pulp exquisiteness, seed is minimum, and edible rate is 100%, light fragrance, sweet and sour palatability is for eating good merchantable brand raw.In the blueberry fruit except sugar, acid and the V of routine
CBe rich in V outward,
E, V
A, V
B, mineral elements such as SOD, Arbutin, protein, anthocyan, dietary fibre and abundant potassium, iron, zinc, calcium.According to analysis, anthocyanidin content is up to 163mg/100g fresh fruit, V in the fruit
EContent 9.3 μ g/100g fresh fruits, total amino acid content 2.54%.The blueberry fruits nutrition is abundant, contains the plurality kinds of health care composition, and international food and agricultural organization classifies them one of as human five big healthy food.In the cowberry seeds germination process light had special demand, under dark condition, be difficult to germinate, conventional blueberry seedling-cultivating method is the surface of seed broadcasting at soil, and this seedling-cultivating method is because illumination, moisture content and nutrition are unbalanced and be difficult to artificial adjustment, cause growth rate slow, general growing-seedling period is at least 1 year, and emergence rate is low, and seedling is of poor quality.
Summary of the invention
The objective of the invention is to remedy above-mentioned deficiency, provide that a kind of growing-seedling period is short, emergence rate is high, the seedling quality is good, and can carry out a kind of artificial raise seedling method for red bilberry of suitability for industrialized production.
The present invention is achieved in that the cowberry seeds after the sterilization treatment, is inoculated under aseptic condition in the MS medium, cultivates under conditions of tissue culture.
Embodiment
Further specify particular content of the present invention below in conjunction with embodiment.
With the cowberry seeds after the sterilization treatment, under aseptic condition, be inoculated in the MS medium, under conditions of tissue culture, cultivate.For the sterilization effect that improves seed with avoid that seed comes to harm in sterilization process, during the cowberry seeds sterilization treatment, can gather ripe blueberry fruit earlier, be preferably the blueberry fruit of 6～9 maturations, after earlier soaking 10～30 fens kinds with detergent liquid, with flowing water towards Xian 0.5～1.5 hour, on superclean bench with 70% ethanol disinfection 10～30 seconds, aseptic water washing 2～6 times, 0.1%HgCl
2Sterilized 5～10 minutes, aseptic water washing 2～6 times behind the suck dry moisture, under aseptic condition, is inoculated into the broken seed that takes out of blueberry fruit in the MS medium, is positioned over tissue culture room, cultivates under conditions of tissue culture.Most of seed begins to germinate after 20～30 days, and germination rate can reach more than 60%, and 50～60 days long later on to 4～6 centimetres, carries out acclimatization and transplants by the mode of tissue cultivating seedling.
The invention solves the problem that in the cowberry seeds germination process light is had specific demand, simple to operate, shortened growing-seedling period greatly.In the seed sterilization process, improve the sterilization effect of seed and avoided seed to come to harm, made the percentage of seedgermination height, become seedling sturdy, easily transplant survival.The present invention can carry out batch production production, brings out a large amount of high-quality seedlings in the short period of time.
Claims (3)
1, a kind of artificial raise seedling method for red bilberry is characterized in that the cowberry seeds after the sterilization treatment, is inoculated under aseptic condition in the MS medium, cultivates under conditions of tissue culture.
2, artificial raise seedling method for red bilberry according to claim 1 after it is characterized in that gathering ripe blueberry fruit and carrying out sterilization treatment, under aseptic condition, is inoculated into the broken seed that takes out of blueberry fruit in the MS medium.
3, artificial raise seedling method for red bilberry according to claim 2, the maturity that it is characterized in that gathering the blueberry fruit is 6～9 maturations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710065992 CN101077045A (en) | 2007-06-26 | 2007-06-26 | Artificial raise seedling method for red bilberry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710065992 CN101077045A (en) | 2007-06-26 | 2007-06-26 | Artificial raise seedling method for red bilberry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101077045A true CN101077045A (en) | 2007-11-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710065992 Pending CN101077045A (en) | 2007-06-26 | 2007-06-26 | Artificial raise seedling method for red bilberry |
Country Status (1)
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| CN (1) | CN101077045A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822219B (en) * | 2010-05-12 | 2011-09-21 | 通化师范学院 | Method for directly inducing and quickly propagating bog bilberry root nodule in test tube |
| CN107182354A (en) * | 2017-06-30 | 2017-09-22 | 黑龙江省带岭林业科学研究所 | A kind of method for improving great bilberry nursery planting percent |
-
2007
- 2007-06-26 CN CN 200710065992 patent/CN101077045A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822219B (en) * | 2010-05-12 | 2011-09-21 | 通化师范学院 | Method for directly inducing and quickly propagating bog bilberry root nodule in test tube |
| CN107182354A (en) * | 2017-06-30 | 2017-09-22 | 黑龙江省带岭林业科学研究所 | A kind of method for improving great bilberry nursery planting percent |
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Open date: 20071128 |