CN101023353B - 将危险分层次的方法与组合物 - Google Patents
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Abstract
Description
表1.增强作用研究的通用方案 |
1)将细胞悬浮在小体积的不含血清H3000stem span培养基中。 |
2)计数细胞。每份样品应存在最少20,000,000个活细胞(用于四种核心染色),加5百万个每份保存样品。 |
3)如下所示(实验布局),将细胞稀释至6.2×105个细胞/100uL,将各100uL等份加入96-孔板。 |
4)将刺激物以10ng/mL加入各列细胞。37℃刺激15分钟。 |
5)为终止刺激,向各孔加入10uL32%PFA固定细胞(室温,5分钟)。 |
6)沉淀细胞,重悬在甲醇中渗透处理。此时可保存细胞3-6周,或立即用标记的抗体染色并分析。 |
7)与实验设计相似,评估结(行)和状态(列)的两维阵列应答反应图。一般取[MFI刺激的/MFI未刺激的]的log2。就基础结而言,可利用log2[MFI基础 x/MFI肿瘤样品最低基础]将测量值置于相同的范围。 |
8)为鉴定生物特征,收集所有肿瘤样品的结状态,研究肿瘤的方差。就多药物时间点而言,各时间点的样品视作不同样品。方差大于在正常细胞中所见的这些结状态通常包括在生物特征中。 |
9)利用生物特征结状态的无监督归类来分组样品。 |
10)采用统计学显著性检验测定得到各组中临床参数的分布(对以前确定的假设进行卡方与斯氏t检验)。 |
11)给予具有相似增强作用的患者样品组名称。 |
12)基于观察到的所有可能途径的相互作用,根据各组的观察情况使途径图加 亮与变暗。总之,如果某组中一半以上样品显示增强作用超过所有样品的中值,则使该途径加亮。否则使该途径变暗。 |
13)根据各样品观察到的增强情况为各患者构建途径图,根据这些图将某患者样品表型分类属于某组(预计具有与该组相似的临床结局)。 |
14)制作由生物特征结状态构成的该疾病的预测图,以类似于原始(样品)的另一组患者样品验证。最大限度减小并优化此图,从而使之尽可能精确并具有尽 可能少的某给定样品的结状态测量值。 |
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US10/898,734 US7393656B2 (en) | 2001-07-10 | 2004-07-21 | Methods and compositions for risk stratification |
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PCT/US2005/026026 WO2006012507A2 (en) | 2004-07-21 | 2005-07-21 | Methods and compositions for risk stratification |
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EP (2) | EP1771728A2 (zh) |
JP (3) | JP2008507286A (zh) |
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US7393656B2 (en) * | 2001-07-10 | 2008-07-01 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for risk stratification |
WO2003067210A2 (en) * | 2001-07-10 | 2003-08-14 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for detecting the activation state of the multiple proteins in single cells |
US7381535B2 (en) * | 2002-07-10 | 2008-06-03 | The Board Of Trustees Of The Leland Stanford Junior | Methods and compositions for detecting receptor-ligand interactions in single cells |
US20030228703A1 (en) * | 2002-04-05 | 2003-12-11 | The Regents Of The University Of Michigan | Fluorescence resonance energy transfer quantitation and stoichiometry in living cells |
WO2005120571A2 (en) * | 2004-06-07 | 2005-12-22 | Ramot At Tel Aviv University Ltd. | Method of passive immunization against disease or disorder characterized by amyloid aggregation with diminished risk of neuroinflammation |
US20060141549A1 (en) * | 2004-08-03 | 2006-06-29 | Sudipta Mahajan | Cell-based kinase assay |
US20080069772A1 (en) * | 2004-08-26 | 2008-03-20 | Eberhard-Karls-Universitaet Tuebingen Universitaetsklinikum | Treatment of transformed or infected biological cells |
EP1634603A1 (de) * | 2004-08-26 | 2006-03-15 | Eberhard-Karls-Universität Tübingen Universitätsklinikum | Behandlung von transformierten oder infizierten biologischen Zellen |
CA2593355A1 (en) * | 2005-01-24 | 2006-07-27 | The Board Of Trustees Of The Leland Stanford Junior University | Method for modeling cell signaling systems by means of bayesian networks |
US20130210034A1 (en) * | 2005-11-04 | 2013-08-15 | Beckman Coulter, Inc. | Complex phosphoprotein activation profiles |
US20070105165A1 (en) * | 2005-11-04 | 2007-05-10 | Charles Goolsby | Composite profiles of cell antigens and target signal transduction proteins for analysis and clinical management of hematologic cancers |
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CN101802182A (zh) * | 2007-08-21 | 2010-08-11 | 诺达利蒂公司 | 用于诊断、预后和治疗方法的方法 |
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WO2006012507A2 (en) | 2006-02-02 |
JP2008507286A (ja) | 2008-03-13 |
US7393656B2 (en) | 2008-07-01 |
US20080254489A1 (en) | 2008-10-16 |
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WO2006012507A3 (en) | 2006-10-12 |
EP1771728A2 (en) | 2007-04-11 |
US20100221750A1 (en) | 2010-09-02 |
US20110201018A1 (en) | 2011-08-18 |
JP2015072286A (ja) | 2015-04-16 |
EP2302379A1 (en) | 2011-03-30 |
US8206939B2 (en) | 2012-06-26 |
CN101023353A (zh) | 2007-08-22 |
US8309316B2 (en) | 2012-11-13 |
JP2014055964A (ja) | 2014-03-27 |
US20110207149A1 (en) | 2011-08-25 |
US20090081699A1 (en) | 2009-03-26 |
US8865420B2 (en) | 2014-10-21 |
US20050112700A1 (en) | 2005-05-26 |
US7939278B2 (en) | 2011-05-10 |
US20080182262A1 (en) | 2008-07-31 |
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