CN100998779A

CN100998779A - Traditional Chinese medicine granule for relieving exterior syndrome removing dampness and regulating the stomach and its preparing method

Info

Publication number: CN100998779A
Application number: CN 200610011174
Authority: CN
Inventors: 李祖泽; 施少斌; 郑荣波; 沈军; 黄晓丹
Original assignee: WANGLAOJI PHARMACEUTICAL CO Ltd GUANGZHOU CITY
Current assignee: WANGLAOJI PHARMACEUTICAL CO Ltd GUANGZHOU CITY
Priority date: 2006-01-12
Filing date: 2006-01-12
Publication date: 2007-07-18
Anticipated expiration: 2026-01-12
Also published as: CN100998779B

Abstract

A Chinese medicine in the form of particle for relieving exterior syndrome, dispelling dampness and regulating stomach is prepared from 16 Chinese-medicinal materials including uncaria stem with hooks, magnolia bark, trichosanthes root, tuckahoe, etc. Its preparing process is also disclosed.

Description

A kind of have induce sweat, dry and in Chinese medicine granules and preparation method thereof

Technical field

The present invention relates to field of medicaments, particularly relate to a kind of treatment gastrointestinal disease Chinese medicine granules and preparation method thereof.

Background technology

Stomachache, diarrhoea, the food acid eructation of choking, nausea and vomiting, gastrointestinal upset, dyspepsia, the dizzy wave of journey, pestilent cold, fever and headache are clinical common symptoms, and these symptoms can cause by multiple reason, and the double sense wind and cold of regarding sb. as an outsider of gastrointestinal disease is one of the main reasons wherein.In Song dynasty " formulary of peaceful benevolent dispensary " these symptoms are put down in writing to some extent, and the prescription of ageratum side as treatment is provided.Ageratum side is described as " heat-damp in summer panacea " by ancient Chinese medicine doctor.This side have induce sweat, damp eliminating and in effect, be usually used in treating the treatment of affection of exogenous wind-cold, internal injury humidity hysteresis, stomachache vomiting and diarrhoea clinically, mainly be with stomachache, diarrhoea, the food acid eructation of choking, nausea and vomiting, gastrointestinal upset: dyspepsia, the dizzy wave of journey, pestilent cold, fever and headache etc. are clinical manifestation, also are widely used at present the treatments such as common cold of gastrointestinal type, acute chronic enteritis of modern medicine.Health pill is the novel formulation that develops on the basis of the former technology of the positive invigorating oral liquid of traditional Radix agastaches, and China's " pharmacopeia " includes it wherein already.Health pill have induce sweat, damp eliminating and in function; Be mainly used in stomachache, diarrhoea, the food acid eructation of choking, nausea and vomiting, gastrointestinal upset: dyspepsia, the dizzy wave of journey, pestilent cold, the treatment of fever and headache.The Health pill of putting down in writing in the pharmacopeia adopts the method for making of " each pulverizing medicinal materials; general ball; coating ", thereby cause Health pill to exist the medicine active ingredient in gastrointestinal dissolution rate and absorbance reduction, soak time prolongs, onset is slow, and because Health pill bad smell when taking can make the patient produce resentment.

Tablet grows up on pill uses the basis, and that tablet has become is wide in variety, output is big, purposes is wide, uses and stores and transports conveniently, one of steady quality dosage form, tablet all accounts for more than 1/3 in the preparation that the pharmacopeia of China and other many countries is recorded, and its application is very wide.Tablet has the following advantages: dosage is accurate; Steady quality; Carry, transport, take more convenient; Mechanization is produced, and output is big, and cost is low, and " sanitary standard " also reaches easily.

A kind of as in the preparation of soft capsule has following characteristics: 1. product appearance novel form, tempting.The capsular packing of this graininess, concerning medicine, mouthfeel is good, easily swallows, and prevents personation.Mode of appearance is different, and rich color is very tempting, to the very attractive and novel sense of consumer.2. safe to carry, easy to use.The packing characteristics of this series products, the people that go out are separately carried according to consumption becomes possibility; In addition, its packing is difficult for broken, carries also fool proof; When being suitable for the daily house of consumer and using, be more suitable for people and be away on a vacation, using when tourism and field work.

Chinese medicine granules is a kind of dosage form that grows up on the basis of dosage forms such as Chinese medicine decoction and dry syrup, has both kept decoction to absorb soon, act on characteristics rapidly, has overcome decoction again and has faced time spent decoction inconvenience, big, the perishable shortcoming of dose.It is little to have dosage, takes, and carries the advantage that the storage transportation is more convenient.

Summary of the invention

The purpose of this invention is to provide a kind of Chinese medicine granules for the treatment of gastrointestinal disease.

Another object of the present invention provides a kind of preparation method for the treatment of the gastrointestinal disease active component.

Another object of the present invention provides a kind of preparation method for the treatment of the Chinese medicine granules of gastrointestinal disease.

Pharmaceutical composition of the present invention is by making in Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Trichosanthis, Poria, the Radix Angelicae Dahuricae, Guangdong Massa Medicata Fermentata, Fructus Tribuli, Rhizoma Atractylodis, Radix Puerariae, Exocarpium Citri Grandis, Fructus Oryzae Germinatus, Flos Chrysanthemi, the Radix Aucklandiae, Herba Pogostemonis, Herba Menthae, the Semen Coicis.

The consumption of each component of medicine of the present invention and proportioning obtain with Test Summary by groping in a large number through the inventor, and each component raw material medicine all has curative effect preferably at the following weight parts ratio range: 15.50～55.80 parts of Ramulus Uncariae Cum Uncis, 80.40～215.50 parts of Cortex Magnoliae Officinalis, 55.50～205.80 parts of Radix Trichosanthis, 155.60～423.20 parts in Poria, 80.40～215.50 parts of the Radixs Angelicae Dahuricae, 80.40～215.50 parts of Guangdong Massa Medicata Fermentata, 15.50～55.80 parts of Fructus Tribulis, 80.40～215.50 parts of Rhizoma Atractylodis, 80.40～215.50 parts of Radix Puerariaes, 36.70～125.40 parts of Exocarpium Citri Grandises, 55.50～205.80 parts of Fructus Oryzae Germinatus, 36.70～125.40 parts of Flos Chrysanthemis, 80.40～215.50 parts of the Radix Aucklandiae, 80.40～215.50 parts of Herba Pogostemonis, 36.70～125.40 parts of Herba Menthaes, 95.20～324.80 parts of Semen Coiciss.

Preferred each component raw material medicine of pharmaceutical composition of the present invention all has curative effect preferably at the following weight parts ratio range: 27.17～41.20 parts of Ramulus Uncariae Cum Uncis, 109.12～163.68 parts of Cortex Magnoliae Officinalis, 81.80～123.17 parts of Radix Trichosanthis, 217.94～327.85 parts in Poria, 109.12～165.56 parts of the Radixs Angelicae Dahuricae, 109.12～165.56 parts of Guangdong Massa Medicata Fermentata, 27.17～41.20 parts of Fructus Tribulis, 109.12～163.68 parts of Rhizoma Atractylodis, 109.12～163.68 parts of Radix Puerariaes, 54.48～81.73 parts of Exocarpium Citri Grandises, 81.80～123.17 parts of Fructus Oryzae Germinatus, 54.48～81.73 parts of Flos Chrysanthemis, 109.12～163.68 parts of the Radix Aucklandiae, 109.12～163.6 parts of Herba Pogostemonis, 54.48～81.73 parts of Herba Menthaes, 136.77～206.10 parts of Semen Coiciss.

Best each component raw material medicine of pharmaceutical composition of the present invention all has curative effect preferably in the following weight parts scope: 34.10 parts of Ramulus Uncariae Cum Uncis, 136.40 parts of Cortex Magnoliae Officinalis, 102.30 parts of Radix Trichosanthis, 272.80 parts in Poria, 136.40 parts of the Radixs Angelicae Dahuricae, 136.40 parts of Guangdong Massa Medicata Fermentata, 34.10 parts of Fructus Tribulis, 136.40 parts of Rhizoma Atractylodis, 136.40 parts of Radix Puerariaes, 68.20 parts of Exocarpium Citri Grandises, 102.30 parts of Fructus Oryzae Germinatus, 68.20 parts of Flos Chrysanthemis, 136.40 parts of the Radix Aucklandiae, 136.40 parts of Herba Pogostemonis, 68.20 parts of Herba Menthaes, 170.50 parts of Semen Coiciss.

The preparation of the active component of the present composition and preparation can adopt following method: one or several use in conjunction preparations in extraction, water extraction, decoction and alcohol sedimentation technique, extraction, infusion process, percolation, reflux extraction, continuous backflow extraction method, the macroreticular resin absorbing method.For example, these crude drug pulverize mix homogeneously can be made powder takes after mixing it with water; Also can be with these medicines decocting together, the condensed water decocting liquid is made oral liquid then.

Medicine of the present invention can will be made any preparation on the pharmaceutics.Active component of the present invention can be made any preparation on the pharmaceutics; The active component of medicine of the present invention can add various conventional adjuvant required when preparing different dosage form, be prepared into any common formulations as disintegrating agent, lubricant, binding agent etc. with the method for Chinese medicinal of routine, as tablet, sugar coated tablet, film coated tablet, enteric coated tablet, powder, capsule, soft capsule, pill, electuary, oral liquid, granule, drop pill, micropill, intramuscular injection agent, instillation, unguentum, ointment, plaster etc.; The best is made tablet, drop pill, oral liquid, granule, capsule, soft capsule.But in order to make each crude drug of this medicine bring into play drug effect better, the present invention preferably adopts following technology to extract active component to raw material, make granule, but this can not limit protection scope of the present invention.

The weight portion proportioning that following medicine activity component extracts raw materials used medicine can be any proportioning, and preferred feedstock medicine weight portion proportioning is a dosage range provided by the present invention.

Medicinal granule preparation method of the present invention comprises the steps:

A. it is standby to get crude drug proportioning Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Trichosanthis, Poria, the Radix Angelicae Dahuricae, Guangdong Massa Medicata Fermentata, Fructus Tribuli, Rhizoma Atractylodis, Radix Puerariae, Exocarpium Citri Grandis, Fructus Oryzae Germinatus, Flos Chrysanthemi, the Radix Aucklandiae, Herba Pogostemonis, Herba Menthae, Semen Coicis;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil dextrin embedding gets cyclodextrin inclusion compound; Aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water, the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction, merge extractive liquid,, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount; Merge above-mentioned concentrated solution, drying is pulverized, and gets the dry extract fine powder;

C. with dry extract fine powder and cyclodextrin inclusion compound mix homogeneously, preparation cost invention medicine activity component.

Preferred medicinal granule preparation method of the present invention comprises the steps:

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and the embedding of volatile oil dextrin gets cyclodextrin inclusion compound, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 1～4 time, each 0.5～5 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 1～4 time, merge extractive liquid,, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount; Merge above-mentioned concentrated solution, drying is pulverized, and gets the dry extract fine powder;

Best medicinal granule preparation method of the present invention comprises the steps:

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets cyclodextrin inclusion compound with a certain amount of dextrin embedding, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 1～3 time, each 0.5～3 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 1～3 time, merge extractive liquid,, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount; Merge above-mentioned concentrated solution, drying is pulverized, and gets the dry extract fine powder;

Medicinal granule preparation method of the present invention, wherein among the step b volatile oil with the dextrin embedding of 2～9 times of amounts; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae are with 30～99% ethanol extractions.

Preferred medicinal granule preparation method two of the present invention, wherein the volatile oil among the step b is with the dextrin embedding of 3～8 times of amounts; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae are with 50～95% ethanol extractions.

Medicinal granule preparation method of the present invention, wherein the dextrin among the step b is a betacyclodextrin.

Medicinal granule preparation method of the present invention, wherein the dry extract that makes among the step c, cyclodextrin inclusion compound mix with the adjuvant that granule is used always, make granule according to the conventional preparation method of granule.

Preferred medicinal granule preparation method of the present invention, wherein the dry extract that makes among the step c, cyclodextrin inclusion compound mix with the adjuvant that granule is used always, described adjuvant is the one or more combination in sucrose, steviosin, lactose, dextrin, starch, ethanol, the polyvinylpyrrolidone, makes granule.

Prepare medicinal granule method two of the present invention:

Medicinal granule preparation method two of the present invention comprises the steps:

B. get the Radix Aucklandiae, Herba Pogostemonis, Cortex Magnoliae Officinalis, Rhizoma Atractylodis, the Radix Angelicae Dahuricae, Herba Menthae 6 flavors, be ground into coarse powder, place the extractor of supercritical CO 2 device to extract respectively, get extract; Extract is mixed, add the cyclodextrin inclusion compound of 1～10 times of extract amount, get cyclodextrin clathrate, standby; Medicinal residues combine, supersound extraction, and 25～90 ℃ of temperature are extracted each 0.2～3 hour 1～3 time; Filter, get supersound extraction liquid, standby; Medical materials such as hook taking rattan, Exocarpium Citri Grandis, Radix Puerariae, Poria, Flos Chrysanthemi, Fructus Tribuli, Radix Trichosanthis, Semen Coicis, Guangdong Massa Medicata Fermentata add 4～12 times of water gagings and soak, and heating decocts three times, filters, filtrate and supersound extraction liquid merge, and concentrate, and high speed centrifugation concentrates, spray drying gets dry extract, and is standby; Get the Fructus Oryzae Germinatus of crude drug proportioning, be ground into coarse powder after, put continuous pulverizing in the micron ultra shifter, the above Fructus Oryzae Germinatus superfine powder of 400 orders, standby;

C. get above-mentioned dry extract, cyclodextrin inclusion compound, Fructus Oryzae Germinatus superfine powder and granule adjuvant commonly used and granulate,, promptly get granule particle drying.

Preferred medicinal granule preparation method two of the present invention comprises the steps:

B. get the Radix Aucklandiae, Herba Pogostemonis, Cortex Magnoliae Officinalis, Rhizoma Atractylodis, the Radix Angelicae Dahuricae, Herba Menthae 6 flavors, be ground into coarse powder, place the extractor of supercritical CO 2 device to extract respectively, get extract; Extract is mixed, add the cyclodextrin inclusion compound of 2～7 times of extract amounts, get cyclodextrin clathrate, standby; Medicinal residues combine, supersound extraction, and 35～80 ℃ of temperature are extracted each 0.5～2 hour 2 times; Filter, get supersound extraction liquid, standby; Medical materials such as hook taking rattan, Exocarpium Citri Grandis, Radix Puerariae, Poria, Flos Chrysanthemi, Fructus Tribuli, Radix Trichosanthis, Semen Coicis, Guangdong Massa Medicata Fermentata add 6～10 times of water gagings and soak, and heating decocts three times, filters, filtrate and supersound extraction liquid merge, and concentrate, and high speed centrifugation concentrates, spray drying gets dry extract, and is standby; Get the Fructus Oryzae Germinatus of crude drug proportioning, be ground into coarse powder after, put continuous pulverizing in the micron ultra shifter, the above Fructus Oryzae Germinatus superfine powder of 400 orders, standby;

C. get a kind of of above-mentioned dry extract, cyclodextrin inclusion compound, Fructus Oryzae Germinatus superfine powder and cane sugar powder, dextrin, soluble starch or polyvinylpyrrolidone or their combination, mixing is granulated, and drying promptly gets finished granule.

Best medicinal granule preparation method two of the present invention comprises the steps:

B. get the Radix Aucklandiae, Herba Pogostemonis, Cortex Magnoliae Officinalis, Rhizoma Atractylodis, the Radix Angelicae Dahuricae, Herba Menthae 6 flavors, be ground into coarse powder, place the extractor of supercritical CO 2 device to extract respectively, get extract; Extract is mixed, add the cyclodextrin inclusion compound of 5 times of extract amounts, get cyclodextrin clathrate, standby; Medicinal residues combine, supersound extraction, and 60 ℃ of temperature are extracted each 1 hour 2 times; Filter, get supersound extraction liquid, standby; Medical materials such as hook taking rattan, Exocarpium Citri Grandis, Radix Puerariae, Poria, Flos Chrysanthemi, Fructus Tribuli, Radix Trichosanthis, Semen Coicis, Guangdong Massa Medicata Fermentata add 8 times of water gagings and soak, and heating decocts three times, filters, filtrate and supersound extraction liquid merge, and concentrate, and high speed centrifugation concentrates, spray drying gets dry extract, and is standby; Get the Fructus Oryzae Germinatus of crude drug proportioning, be ground into coarse powder after, put continuous pulverizing in the micron ultra shifter, the above Fructus Oryzae Germinatus superfine powder of 400 orders, standby;

C. get a kind of of above-mentioned dry extract, cyclodextrin inclusion compound, Fructus Oryzae Germinatus superfine powder and cane sugar powder, dextrin, polyvinylpyrrolidone or their combination, mixing is granulated, and drying promptly gets finished granule.

Prepare drug particles agent method three of the present invention:

Medicinal granule preparation method three of the present invention comprises the steps;

B. above ten Six-elements, Herba Pogostemonis, the Radix Aucklandiae, Herba Menthae, Rhizoma Atractylodis extract volatile oil with steam distillation respectively, and volatile oil device is in addition preserved, and medicinal residues decoct with water 1～4 time, and each 0.2～3 hour, filter, merging filtrate is concentrated into the thick paste shape, and is standby; Massa Medicata Fermentata, Poria decoct with water respectively 1～4 time, and each 0.2～3 hour, filter, merging filtrate, filtrate is concentrated in right amount, and add ethanol and make and contain the alcohol amount and reach 30～65%, standing over night, filtration, filtrate recycling ethanol is concentrated into the thick paste shape, and is standby; Fructus Oryzae Germinatus decocts with water 1～4 time, and each 0.2～1.5 hour, filter, merging filtrate is concentrated into the thick paste shape, and is standby; Cortex Magnoliae Officinalis, Fructus Tribuli, Flos Chrysanthemi decoct with water respectively 1～4 time, and each 0.2～3 hour, filter, merging filtrate is concentrated into the thick paste shape, and is standby; The Radix Angelicae Dahuricae, Exocarpium Citri Grandis, Radix Puerariae, Radix Trichosanthis extract 1～4 time with 50～85% alcohol heating reflux respectively, and each 0.5～3.5 hour, filter, merging filtrate reclaims ethanol, is concentrated into the thick paste shape, and is standby; Ramulus Uncariae Cum Uncis extracts 1～4 time with 50～85% alcohol heating reflux, and each 0.5～3.5 hour, filter, merging filtrate with hydrochloric acid adjust pH to 3～4, fully stirs, and filters, and filtrate is concentrated into the thick paste shape with 6～14% sodium carbonate liquor adjust pH to 6～7, and is standby; Semen Coicis is ground into fine powder 50～95 ℃ of oven dry, adds above-mentioned thick paste and an amount of starch, and fully mixing adds the volatile oil with an amount of dissolve with ethanol, and drying gets dry extract;

C. get above-mentioned dry extract and granule adjuvant commonly used at present, make granule according to present conventional granulates agent preparation method.

Preferred medicinal granule preparation method three of the present invention comprises the steps;

B. above ten Six-elements, Herba Pogostemonis, the Radix Aucklandiae, Herba Menthae, Rhizoma Atractylodis extract volatile oil with steam distillation respectively, and volatile oil device is in addition preserved, and medicinal residues decoct with water 2～3 times, and each 0.5～2 hour, filter, merging filtrate is concentrated into the thick paste shape, and is standby; Massa Medicata Fermentata, Poria decoct with water respectively 2～3 times, and each 0.5～2 hour, filter, merging filtrate, filtrate is concentrated in right amount, and add ethanol and make and contain the alcohol amount and reach 35～55%, standing over night, filtration, filtrate recycling ethanol is concentrated into the thick paste shape, and is standby; Fructus Oryzae Germinatus decocts with water 2～3 times, and each 0.2～1 hour, filter, merging filtrate is concentrated into the thick paste shape, and is standby; Cortex Magnoliae Officinalis, Fructus Tribuli, Flos Chrysanthemi decoct with water respectively 2～3 times, and each 0.5～2 hour, filter, merging filtrate is concentrated into the thick paste shape, and is standby; The Radix Angelicae Dahuricae, Exocarpium Citri Grandis, Radix Puerariae, Radix Trichosanthis extract 2～3 times with 60～80% alcohol heating reflux respectively, and each 1～3 hour, filter, merging filtrate reclaims ethanol, is concentrated into the thick paste shape, and is standby; Ramulus Uncariae Cum Uncis extracts 2～3 times with 60～80% alcohol heating reflux, and each 1～3 hour, filter, merging filtrate with hydrochloric acid adjust pH to 3～4, fully stirs, and filters, and filtrate is concentrated into the thick paste shape with 8～12% sodium carbonate liquor adjust pH to 6～7, and is standby; Semen Coicis is ground into fine powder 60～90 ℃ of oven dry, adds above-mentioned thick paste and an amount of starch, and fully mixing adds the volatile oil with an amount of dissolve with ethanol, and drying gets dry extract;

Best medicinal granule preparation method three of the present invention comprises the steps;

B. above ten Six-elements, Herba Pogostemonis, the Radix Aucklandiae, Herba Menthae, Rhizoma Atractylodis extract volatile oil with steam distillation respectively, and volatile oil device is in addition preserved, and medicinal residues decoct with water secondary, and 1.5 hours for the first time, 1 hour for the second time, filter, merging filtrate is concentrated into the thick paste shape, and is standby; Massa Medicata Fermentata, Poria decoct with water secondary respectively, and each 1.5 hours, filter, merging filtrate, filtrate is concentrated in right amount, and add ethanol and make and contain the alcohol amount and reach 45%, standing over night, filtration, filtrate recycling ethanol is concentrated into the thick paste shape, and is standby; Fructus Oryzae Germinatus decocts with water secondary, and each 0.5 hour, filter, merging filtrate is concentrated into the thick paste shape, and is standby; Cortex Magnoliae Officinalis, Fructus Tribuli, Flos Chrysanthemi decoct with water secondary respectively, and each 1.5 hours, filter, merging filtrate is concentrated into the thick paste shape, and is standby; The Radix Angelicae Dahuricae, Exocarpium Citri Grandis, Radix Puerariae, Radix Trichosanthis extract secondary with 70% alcohol heating reflux respectively, and 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate reclaims ethanol, is concentrated into the thick paste shape, and is standby; Ramulus Uncariae Cum Uncis extracts secondary with 70% alcohol heating reflux, and 2 hours for the first time, 1.5 hours for the second time, filter, merging filtrate with hydrochloric acid adjust pH to 3～4, fully stirs, and filters, and filtrate is concentrated into the thick paste shape with 10% sodium carbonate liquor adjust pH to 6～7, and is standby; Semen Coicis is ground into fine powder 80 ℃ of oven dry, adds above-mentioned thick paste and an amount of starch, and fully mixing adds the volatile oil with an amount of dissolve with ethanol, and drying gets dry extract;

C. get above-mentioned dry extract, cyclodextrin inclusion compound, Fructus Oryzae Germinatus superfine powder and cane sugar powder, dextrin, mixing is granulated, and drying promptly gets finished granule.

Prepare drug particles agent method four of the present invention:

Medicinal granule preparation method four of the present invention comprises the steps;

B. above ten Six-elements are got the Radix Aucklandiae, Rhizoma Atractylodis, Herba Menthae, Herba Pogostemonis, Exocarpium Citri Grandis vapor distillation, and it is standby to collect volatile oil; Medicinal residues decoct with water 1～4 time, and each 0.5～3 hour, collecting decoction filtered, and filtrate is concentrated in right amount, put coldly, add ethanol and make and contain the alcohol amount and reach 30～60%, and standing over night filters, and recovery ethanol is concentrated into certain volume, and is standby; Ramulus Uncariae Cum Uncis, Fructus Tribuli, Flos Chrysanthemi, Cortex Magnoliae Officinalis, medicated leaven decoct with water 1～4 time, and each 0.5～3.5 hour, collecting decoction filtered, filtrate is concentrated in right amount, puts coldly, adds ethanol and makes and contain the alcohol amount and reach 30～50%, standing over night, filter, reclaim ethanol and be concentrated into certain volume, standby; Get Semen Coicis, Fructus Oryzae Germinatus decocts with water 1～4 time, each 0.2～2 hour, collecting decoction filtered, filtrate is concentrated in right amount, puts coldly, add ethanol and make and contain the alcohol amount and reach 20～60%, standing over night, recovery ethanol is concentrated into certain volume, and is standby; Get Poria, Radix Trichosanthis, the Radix Angelicae Dahuricae, Radix Puerariae, decoct with water 1～4 time, each 0.2～3.5 hour, collecting decoction filtered, filtrate is concentrated in right amount, puts coldly, adds ethanol and makes and contain the alcohol amount and reach 40～80%, standing over night, filter, reclaim ethanol, be concentrated into an amount of, standby;

C. said extracted liquid is mixed, drying adds volatile oil, gets dry extract; Get above-mentioned dry extract and granule adjuvant commonly used at present, make granule according to present conventional granulates agent preparation method.

Preferred medicinal granule preparation method four of the present invention comprises the steps;

B. above ten Six-elements are got the Radix Aucklandiae, Rhizoma Atractylodis, Herba Menthae, Herba Pogostemonis, Exocarpium Citri Grandis vapor distillation, and it is standby to collect volatile oil; Medicinal residues decoct with water 2～3 times, and each 1～2 hour, collecting decoction filtered, and filtrate is concentrated in right amount, put coldly, add ethanol and make and contain the alcohol amount and reach 40～50%, and standing over night filters, and recovery ethanol is concentrated into certain volume, and is standby; Ramulus Uncariae Cum Uncis, Fructus Tribuli, Flos Chrysanthemi, Cortex Magnoliae Officinalis, medicated leaven decoct with water 2～3 times, and each 1～3 hour, collecting decoction filtered, filtrate is concentrated in right amount, puts coldly, adds ethanol and makes and contain the alcohol amount and reach 35～45%, standing over night, filter, reclaim ethanol and be concentrated into certain volume, standby; Get Semen Coicis, Fructus Oryzae Germinatus decocts with water 2～3 times, each 0.5～1.5 hour, collecting decoction filtered, filtrate is concentrated in right amount, puts coldly, add ethanol and make and contain the alcohol amount and reach 30～50%, standing over night, recovery ethanol is concentrated into certain volume, and is standby; Get Poria, Radix Trichosanthis, the Radix Angelicae Dahuricae, Radix Puerariae, decoct with water 2～3 times, each 0.5～3 hour, collecting decoction filtered, and filtrate is concentrated in right amount, put coldly, added ethanol and made and contain the alcohol amount and reach 50～70%, and standing over night filters, and reclaims ethanol, is concentrated in right amount, and is standby;

C. said extracted liquid is mixed, drying adds volatile oil, gets dry extract; Get above-mentioned dry extract, add a kind of of cane sugar powder, dextrin, soluble starch or polyvinylpyrrolidone or their combination, mixing is granulated, and promptly gets granule.

Best medicinal granule preparation method four of the present invention comprises the steps;

B. above ten Six-elements are got the Radix Aucklandiae, Rhizoma Atractylodis, Herba Menthae, Herba Pogostemonis, Exocarpium Citri Grandis vapor distillation, and it is standby to collect volatile oil; Medicinal residues decoct with water secondary, and each 1.5 hours, collecting decoction filtered, and filtrate is concentrated in right amount, put coldly, add ethanol and make and contain the alcohol amount and reach 45%, and standing over night filters, and recovery ethanol is concentrated into certain volume, and is standby; Ramulus Uncariae Cum Uncis, Fructus Tribuli, Flos Chrysanthemi, Cortex Magnoliae Officinalis, medicated leaven decoct with water secondary, and 2 hours for the first time, 1.5 hours for the second time, collecting decoction, filter, filtrate is concentrated in right amount, puts coldly, adds ethanol and makes and contain alcohol and measure and reach 40%, standing over night filters, and reclaims ethanol and is concentrated into certain volume, and is standby; Get Semen Coicis, Fructus Oryzae Germinatus decocts with water secondary, each 1 hour, collecting decoction filtered, filtrate is concentrated in right amount, puts coldly, add ethanol and make and contain the alcohol amount and reach 45%, standing over night, recovery ethanol is concentrated into certain volume, and is standby; Get Poria, Radix Trichosanthis, the Radix Angelicae Dahuricae, Radix Puerariae, decoct with water secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction, filter, filtrate is concentrated in right amount, puts coldly, adds ethanol and makes and contain alcohol and measure and reach 60%, standing over night, filter, reclaim ethanol, be concentrated into an amount of, standby;

C. get above-mentioned dry extract, add a kind of of cane sugar powder, dextrin, soluble starch or polyvinylpyrrolidone or their combination, mixing is granulated, and drying promptly gets granule.

More than form when producing and to increase or to reduce according to corresponding ratio, as large-scale production can be unit with kilogram or with the ton, small-scale production can be unit with the gram also, and weight can increase or reduce, but the crude drug material weight proportion constant rate between each composition.

The specific embodiment

In order to understand the present invention better, logical below test example is further set forth the beneficial effect of medicine of the present invention.These test examples comprise the clinical efficacy experimental observation of medicine of the present invention.By observing medicine of the present invention wind and cold is held under the arm wet hold under the arm the stagnate flu of card, the influence of acute gastroenteritis, the therapeutical effect of medicine of the present invention is described.Below used medicine of the present invention for the ease of narration, below will be called for short medicine of the present invention according to the medicine of embodiment 21 methods preparation according to the preparation of embodiment 21 methods in the test.

Medicine main pharmacodynamics research data of the present invention

One of pharmacodynamic study: medicine of the present invention is to the influence of mice gastric emptying, intestinal propulsion

1 experiment material

1.1 medicine and reagent

1.1.1 medicine of the present invention: dry medicated powder (not containing excipient), the suitable crude drug amount of every gram 4.1g is provided lot number 20030212 by Wanglaoji Pharmaceutical Co., Ltd., Guangzhou City.Mice little, in, heavy dose of 10 times, 20 times, 30 times designs by clinical adult (60kg) consumption per day (0.185g/kg), be equivalent to crude drug amount: 1.85g/kg, 3.70g/kg, 5.55g/kg respectively.Medicine is made into 92.5g/L, 185g/L, 277.5g/L with distilled water, and the administration volume is 20mL/kg, adopts gastric infusion.

1.1.2 BAOJIWAN: dry medicated powder (not containing excipient) is provided lot number: 20030212 by Yangcheng Medicine Co., Ltd., Guangzhou City.The dosage of mice is by 10 times, 20 times designs of clinical adult (60kg) consumption per day (0.185g/kg), and dosage is: 1.85g/kg, 3.70g/kg.Medicine is made into 92.5g/L, 185g/L with distilled water, and the administration volume is 20mL/kg, adopts gastric infusion.

1.1.3 fourth woods: tablet, every 10mg, Xian-Janssen Pharmaceutical Ltd. produces, lot number: 990825009.Proportionately 10 times of designs of people's consumption of the dosage of mice are 5mg/kg, and medicine is made into 0.25mg/ml with distilled water, adopt gastric infusion.

1.1.4 cisapride: tablet, every 5mg, Xian-Janssen Pharmaceutical Ltd. produces, lot number: 991021107.Proportionately 10 times of designs of people's consumption of the dosage of mice are 2.5mg/kg, and medicine is made into 0.125mg/mL with distilled water, adopt gastric infusion.

1.1.5 dopamine hydrochloride inj: 20mg/2mL, Jintan City, Jiangsu Province pharmaceutical factory produces, lot number: 0202221, the dosage that mice is adopted is 5mg/kg, and medicine is 0.5mg/mL with the normal saline dilution, and the administration volume is 10ml/kg, adopts intraperitoneal injection.

1.1.6 adrenalin hydrochloride injection: every milliliter contains 1mg, Mingxing Pharmaceutical Factory, Guangzhou production, and lot number: 021102, the mice dosage is 0.5mg/kg, and being diluted to concentration with normal saline is 0.05mg/mL, and hypodermic volume is the 0.1mg/10g body weight.

1.1.7 atropine sulfate injection: every milliliter contains 0.5mg, and people pharmaceutical Co. Ltd of Guangdong nation produces, lot number: 020303, the dosage of mice is 0.5mL/kg, be diluted to 0.05mg/mL with normal saline, volume injected is the 0.1mL/10g body weight, adopts subcutaneous injection.

1.1.8 the neostigmine methylsulfate injection: 2mL contains 1mg, Shanghai Xinyi Pharmaceutical Plant produces, lot number: 021003, and the dosage of mice is 0.12mg/kg, is diluted to 0.012mg/mL with normal saline, volume injected is the 0.1mL/10g body weight, adopts subcutaneous injection.

1.1.9 the metoclopramide sheet, every 5mg, Jiangsu Pengyao Pharmaceutical Co., Ltd. produces, lot number: 010618, mice dosage is 10mg/kg, medicine is made into 0.5mg/mL with distilled water, adopts gastric infusion.

1.2 animal: the NIH mice, 18～23g, the cleaning level is provided the quality certification: 2002A028 by Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center.

2 experimental techniques and result

2.1 medicine of the present invention is to the influence of normal mouse gastric emptying ^[1]

Get above-mentioned specification mice, random packet, that is: distilled water group, medicine of the present invention little, in, heavy dose of group, little, the middle dosage group of Health pill, cisapride group.Except that the cisapride group, the administration treated animal was by above-mentioned dosage administration in advance 3 days, and every day 1 time, the distilled water group is given the equal-volume distilled water.Fasting is 16 hours before the last administration, after the administration 1 hour, behind the cisapride single administration 0.5 hour, quantitatively irritates stomach nutrition and sticks with paste and (press document ^[1]Method prepares in advance), every 0.6ml, animal is put to death in dislocation after 20 minutes, cuts open the belly to pluck stomach and claim the stomach gross weight with electronic balance, claims the stomach net weight after removing gastric content, deducts the stomach net weight with the stomach gross weight, i.e. and residual quantity is stuck with paste in stomach nutrition.Adopt the t check to compare the statistical significance of administration group and distilled water group.

The results are shown in Table 1, data show in the table, and medicine of the present invention and Health pill are to the effect of normal mouse gastric emptying all not obvious (P＞0.05), and cisapride then has obvious minimizing stomach rice to stick with paste the effect (P＜0.05) of residual quantity.

Table 1, medicine of the present invention are to the influence of normal mouse gastric emptying

Group	Number of animals (n)	Dosage (g/kg)	Stomach rice paste residual quantity (g) (x ± s)
Group	Number of animals (n)	Dosage (g/kg)	Stomach rice paste residual quantity (g) (x ± s)	The heavy dose of Health pill low dose of dosage in the water matched group medicine low dose of the present invention	13 12 12 12 10	Equal-volume distilled water 1.85 3.70 5.55 1.85	0.528±0.117 0.505±0.094 0.445±0.144 0.476±0.091 0.459±0.135

Middle dosage cisapride

10 12

3.70 2.5mg

0.553±0.151 0.401±0.133 ^*

Compare with the distilled water group: ^*P＜0.05

2.2 medicine of the present invention causes the influence of mice delayed gastric emptying to dopamine hydrochloride ^[2]

Get above-mentioned specification mice, random packet, that is: distilled water group, distilled water+dopamine group, little, the middle and high dosage group of medicine of the present invention+dopamine group, little, the middle dosage of Health pill+dopamine group, motilium+dopamine group.Except that the motilium group, the administration treated animal was by above-mentioned dosage administration in advance 3 days, and every day 1 time, distilled water group and distilled water+dopamine group is given the equal-volume distilled water.Fasting is 16 hours before the last administration, and after the administration 1 hour, behind the motilium single administration 0.5 hour, except that the distilled water group, all the other respectively organized equal lumbar injection dopamine, and the distilled water group is then injected the equal-volume normal saline.Quantitatively irritate stomach nutrition after 10 minutes and stick with paste (preparing in advance) by literature method, every 0.6ml, animal is put to death in dislocation after 20 minutes, cut open the belly and pluck stomach and claim the stomach gross weight with electronic balance, claim the stomach net weight after removing gastric content, deduct the stomach net weight with the stomach gross weight, i.e. residual quantity is stuck with paste in stomach nutrition.Adopt the t check to compare the statistical significance of administration group and dopamine group.

The results are shown in Table 2, data show in the table, and residual quantity is stuck with paste in nutrition in distilled water+dopamine group stomach obviously increases (P＜0.01) than the distilled water group, and the prompting dopamine significantly postpones the mice gastric emptying.Medicine low dose of the present invention, dosage group and distilled water in the medicine of the present invention+dopamine group compares, and its stomach nutrition is stuck with paste residual quantity and is obviously reduced (P＜0.01), and pointing out two kinds of dosage medicines of the present invention that dopamine is caused the mice delayed gastric emptying has antagonism.Health pill low dose and motilium show similar effect (P＜0.05 or P＜0.01), but medicine of the present invention and Health pill effect of high dosage are not obvious.

Table 2, medicine of the present invention cause the influence of mice mice delayed gastric emptying to dopamine hydrochloride

Group	Number of animals (n)	Dosage (g/kg)	Stomach rice paste residual quantity (g) (x ± s)
Group	Number of animals (n)	Dosage (g/kg)	Stomach rice paste residual quantity (g) (x ± s)	Distilled water control group distilled water+dopamine medicine of the present invention is little+dopamine medicine of the present invention in+dopamine medicine of the present invention is big+the dopamine Health pill is little+the dopamine Health pill in+dopamine Domperidone+dopamine	16 17 16 16 16 18 18 14	Equal-volume distilled water equal-volume distilled water 1.85 3.70 5.55 1.85 3.70 5mg	0.404±0.099 0.621±0.081 ^## 0.482±0.097 ^ 0.497±0.076 ^ 0.585±0.065 0.522±0.142 ^* 0.586±0.085 0.519±0.104 ^**

Compare with the distilled water group: ^##P＜0.01; Compare with distilled water+dopamine group: ^*P＜0.05, ^*P＜0.01

2.3 medicine of the present invention is to the propulsive influence of small intestine movement of mice

Get above-mentioned specification mice and be divided into 6 groups at random: three dosage groups of medicine of the present invention, two dosage groups of Health pill, metoclopramide group and distilled water matched group, the administration treated animal is by above-mentioned dosage administration in advance 3 days, every day 1 time.Matched group is given the equal-volume distilled water.Fasting is 12 hours before the last administration, 1 hour every animal is irritated suspension (5% charcoal end, stomach charcoal end after the administration, 10% gelatin) 0.2mL, put to death in 20 minutes, take out small intestinal and be tiled on the blank sheet of paper, measure the propulsive length in small intestinal total length and charcoal end, with both percentage ratio as advancing percentage rate, relatively each administration group and matched group significant difference adopt the t check.

The results are shown in Table 3, data show in the table, three dosage of medicine of the present invention, and only low dose has the intestinal propulsion effect (P＜0.05) of acceleration, and two dosage of middle and high dosage and medicine of the present invention are to the propulsive influence of mouse small intestine not obvious (P＞0.05).

Table 3, medicine of the present invention are to the influence of small intestine movement of mice progradation

Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s
Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s	Dosage metoclopramide in the low dose of Health pill of dosage medicine high dose of the present invention Health pill in the low dose of medicine of the present invention of distilled water control group medicine of the present invention	12 12 12 12 12 12 12	Equal-volume distilled water 1.85 3.70 5.55 1.85 3.70 0.01	60.9±15.3 77.4±8.7 ^* 68.8±10.7 64.0±9.3 63.2±11.2 67.4±11.3 78.6±9.8 ^**

Compare with the distilled water matched group: ^*P＜0.05, ^*P＜0.01

2.4 medicine of the present invention advances hyperfunction influence to neostigmine load mouse small intestine ^[3]

Get above-mentioned mice, be divided into 7 groups at random: distilled water matched group, neostigmine model group, three dosage of medicine of the present invention+neostigmine group, two dosage of Health pill+neostigmine group.Administration treated animal administration in advance 3 days, every day 1 time, fasting is 12 hours before the last administration, after the administration 1 hour, model group animal and administration treated animal subcutaneous injection neostigmine (0.12mg/kg), model group and matched group subcutaneous injection equal-volume normal saline, and irritate stomach charcoal end suspension immediately, 20 minutes execution animals, measure the intestinal propulsion percentage rate by last method, adopt the t check, difference comparison model group and matched group, the statistical significance of each administration group and model group.

The results are shown in Table 4, data show in the table, the apparent in view raising of model group intestinal propulsion percentage rate and matched group (P＜0.01), and it is hyperfunction that the prompting neostigmine can cause that mouse small intestine advances.The middle and high dosage of medicine of the present invention and two dosage groups of Health pill intestinal propulsion percentage rate obviously reduce than model group, and the intestinal propulsion of pointing out medicine of the present invention, Health pill that neostigmine is caused is hyperfunction inhibitory action.

Table 4, medicine of the present invention cause mouse small intestine to neostigmine and advance hyperfunction influence

Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s
Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s	Dosage in the low dose of Health pill of dosage medicine high dose of the present invention Health pill in the low dose of medicine of the present invention of distilled water control group model group medicine of the present invention	12 13 12 12 12 11 11	Equal-volume distilled water equal-volume distilled water 1.85 3.70 5.55 1.85 3.70	55.9±5.9 72.7±13.8 ^## 63.8±15.4 19.5±12.9 ^* 58.2±12.3 ^* 62.2±10.6 ^* 59.9±9.3 ^*

Compare with the distilled water matched group: ^##P＜0.01; Compare with model group: ^*P＜0.05

2.5 medicine of the present invention is to the propulsive influence of atropine load mouse small intestine ^[3]

Get above-mentioned specification mice and be divided into 7 groups at random: distilled water matched group, model group, three dosage of medicine of the present invention+atropine group, two dosage of Health pill+atropine group, metoclopramide+atropine group.Administration treated animal administration in advance 3 days, every day 1 time, model group and matched group are given the equal-volume distilled water, fasting is 12 hours before the last administration, after the administration 1 hour, model group and administration treated animal subcutaneous injection atropine (0.5mg/kg), matched group injection equal-volume normal saline.And irritate stomach charcoal end suspension immediately, put to death animal, and measured the intestinal propulsion percentage rate in 20 minutes by last method.Adopt the t check, comparison model group and distilled water matched group, the significant difference of administration group and model group.

The results are shown in Table 5, data show in the table, and model group intestinal propulsion percentage rate obviously reduces (P＜0.01) than matched group, and the prompting atropine can cause the inhibition of intestinal propulsion.Medicine high dose of the present invention and the apparent in view rising of model group (P＜0.05), two dosage of all the other low doses and Health pill and model group be no significant difference (P＞0.05) relatively all.

Table 5, medicine of the present invention cause mouse small intestine to atropine and advance the influence that suppresses

Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s
Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s	Dosage metoclopramide in the low dose of Health pill of dosage medicine high dose of the present invention Health pill in the low dose of medicine of the present invention of distilled water control group model group medicine of the present invention	10 11 11 11 11 11 11 11	Equal-volume distilled water equal-volume distilled water 1.85 3.70 5.55 1.85 3.70 0.01	65.3±16.8 42.9±6.4 ^## 44.1±9.1 43.4±9.2 51.3±8.0 ^* 46.0±14.2 43.8±7.0 55.4±7.6 ^**

Compare with the distilled water matched group: ^##P＜0.01; Compare with model group: ^*P＜0.05, ^*P＜0.01

2.6 medicine of the present invention is to the propulsive influence of epinephrine load mouse small intestine ^[3]

Get above-mentioned specification mice and be divided into 7 groups at random: distilled water matched group, model group, three dosage of medicine of the present invention+epinephrine group, two dosage of Health pill+epinephrine group, metoclopramide+epinephrine group.Administration treated animal administration in advance 3 days, every day 1 time, model group and matched group are given the equal-volume distilled water, fasting is 12 hours before the last administration, after the administration 1 hour, model group and administration treated animal subcutaneous injection epinephrine (0.5mg/kg), matched group injection equal-volume normal saline.And irritate stomach charcoal end suspension immediately, put to death animal, and measured the intestinal propulsion percentage rate in 20 minutes by last method.Adopt the t check, comparison model group and distilled water matched group, the significant difference of administration group and model group.

The results are shown in Table 6, data show in the table, and model group intestinal propulsion percentage rate significantly descends (P＜0.01) than matched group, and the prompting epinephrine can make intestinal propulsion obviously suppress.Three dosage groups of medicine of the present invention advance percentage rate and the apparent in view raising of model group (P＜0.05 or P＜0.01), carry this and show that said preparation causes mouse small intestine to epinephrine and advances inhibition that antagonism is arranged.Two dosage effects of Health pill not obvious (P＞0.05).

Table 6, medicine of the present invention cause mouse small intestine to epinephrine and advance the influence that suppresses

Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s
Group	Number of animals (n)	Dosage (g/kg)	Intestinal propulsion percentage rate (%) x ± s	Dosage metoclopramide in the low dose of Health pill of dosage medicine high dose of the present invention Health pill in the low dose of medicine of the present invention of distilled water control group model group medicine of the present invention	13 12 12 12 12 11 12 12	Equal-volume distilled water equal-volume distilled water 1.85 3.70 5.55 1.85 3.70 0.01	61.0±8.4 39.7±8.4 ^## 48.4±11.1 ^* 48.0±10.6 ^* 53.7±12.1 ^ 44.0±5.7 39.4±8.4 54.3±9.4 ^

Compare with the distilled water matched group: ^##P＜0.01; Compare with model group: ^*P＜0.05; ^*P＜0.01

List of references

1. the Wu Chun good fortune is old many. the discussion of mice gastric emptying model, Chinese Pharmacological circular, 1997; 13 (3): 271

2. Wang Jing, Hou Jiayu. power stomach granule is to the influence of digestive tract power, and world Chinese digests magazine, and 2000; 8 (4): 377

3. your person of outstanding talent of king, Du Qun, Shao Tingyin, etc. the just mechanism research of relieving constipation square tube, Traditional Chinese Medicine University Of Guangzhou's journal, 1999; 16 (2): 145

Two of pharmacodynamic study: medicine antidiarrheal of the present invention, analgesia and spasmolysis test

1 material

1.1 medicine and reagent

1.1.2 BAOJIWAN: dry medicated powder (not containing excipient), provide by Yangcheng Medicine Co., Ltd., Guangzhou City, lot number: 20030212, the dosage of mice is by 10 times, 20 times designs of clinical adult (60kg) consumption per day (0.185g/kg), and dosage is: 1.85g/kg, 3.70g/kg.Medicine is made into 92.5g/L, 185g/L with distilled water, and the administration volume is 20mL/kg, adopts gastric infusion.

1.1.3 ZHENGLUOWAN: 150mg/ grain, Japan Taiko Pharmaceutical Co., Ltd. maker is produced, lot number: 11VA07, build date: 01/2000, Coming-of-Age Day consumption be 22.5mg/kg, the dosage of mice by Coming-of-Age Day consumption 10 times of designs, be 225mg/kg, medicine is made into 11.25mg/mL with distilled water, and the administration volume is 20mL/kg, adopts gastric infusion.

1.1.4 acecoline: analytical pure, Military Medical Science Institute medical supply station, lot number: 20020908.

1.1.5 histamine phosphate: biochemical reagents, Shanghai Inst. of Biochemistry, Chinese Academy of Sciences produces, lot number: 200210291.

1.2 animal: the NIH mice, 18～22g, the cleaning level is provided the quality certification: 2002A028 by Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center; New Zealand white rabbit, 2.0～2.5kg, Baiyun District plant provides the quality certification by Guangzhou fringe north: 2002A010; Albino guinea-pig, body weight 250～350g is provided the quality certification: 2002A062 by No.1 Military Medical Univ.'s Experimental Animal Center.

2 experimental techniques and result

2.1 medicine of the present invention causes the anti-diarrhea effect of diarrhea of mouse model to Folium Sennae ^[1]

Get above-mentioned specification mice and be divided into 7 groups at random: distilled water matched group, the large, medium and small dosage group of medicine of the present invention, in the Health pill, small dose group, the ZHENGLUOWAN group, administration treated animal administration in advance 3 days, every day 1 time, matched group is given the equal-volume distilled water, and fasting is 6 hours before the last administration.After the administration 1 hour, every animal is irritated stomach 70% Folium Sennae water extract 0.4mL, mice is placed on 1 * 1cm screen cloth, on diameter 10cm filler cap, filter paper on the pad off the net, observed 1 time in per 2 hours, counting rushes down the down number of times of feces, rush down down feces and comprise that unshaped is mashed just and loose stool, with 6 hours accumulative total cathartic number of times as evaluation index, relatively the difference of administration group and water matched group adopts the t check analysis.

The results are shown in Table 1, the data in the table show that three dosage groups of medicine of the present invention, the discharge in 6 hours of two dosage treated animals of Health pill are rushed down following number of times just and obviously reduced (P＜0.05 or P＜0.01) than the distilled water matched group.In the medicine of the present invention, second of low dose group and Health pill small dose group 2 hours and the 3rd discharged and rush down down just that number of times also obviously reduces than matched group in 2 hours.Point out medicine of the present invention that anti-diarrhea effect is arranged.

Table 1, medicine of the present invention cause the anti-diarrhea effect (x ± s) of diarrhea of mouse model to Folium Sennae

Group	Number of animals (n)	Dosage (g/kg)	Rush down following just number of times
			Rush down following just number of times				First 2 hours	Second 2 hours	The 3rd 2 hours	Amounted in 6 hours
			ZHENGLUOWAN in the little Health pill of the big Health pill of medicine of the present invention in the control group medicine small capital of the present invention invention medicine	19 18 19 18 18 17 18	Equal-volume water 1.85 3.70 5.55 1.85 3.70 0.225	3.74±2.45 4.56±2.28 4.53±2.39 3.61±2.68 3.94±1.92 2.58±2.5 2.00±2.04 ^*	First 2 hours	Second 2 hours	The 3rd 2 hours	Amounted in 6 hours	5.33±2.54 3.00±1.50 ^3.15±1.68 ^4.00±1.57 3.22±2.29 ^*4.00±1.97 4.67±2.19	2.06±1.21 0.94±1.05 ^0.68±0.95 ^1.67±1.03 0.94±0.80 ^**1.24±1.30 1.92±1.62	10.79±3.03 8.44±1.82 ^*8.37±2.01 ^8.72±2.83 ^8.39±2.57 ^8.76±2.51 ^8.58±1.83 ^

Compare with matched group: ^*P＜0.05; ^*P＜0.01

2.2 the analgesic activity of medicine of the present invention test

2.2.1 acetic acid twisting method ^[2]: get above-mentioned specification mice fasting 12 hours, stratified random grouping and administration are the same, after the administration 1 hour, after the rotundine administration 30 minutes, lumbar injection 0.6% acetic acid 0.1mL/10g body weight, count 20 minutes mouse writhing number of times and writhing response and incubation period occurs, adopt the t check statistical significance of each administration group and matched group relatively respectively.

The results are shown in Table 2, data show in the table, and each administration group and matched group compare, difference that the incubation period of writhing response, there are no significant (P＜0＞0.05); Turn round body number of times medicine high dose group of the present invention and obviously reduce (P＜0.05) than matched group, two dosage groups of Health pill also have certain minimizing, but there was no significant difference (P＞0.05).Point out medicine of the present invention that certain analgesic activity is arranged.

The analgesic activity of table 2, medicine Dichlorodiphenyl Acetate writhing method mice of the present invention (x ± s)

Group	Number of animals (n)	Dosage (g/kg)	Writhing response incubation period (s)	Turn round the body number of times
Group	Number of animals (n)	Dosage (g/kg)	Writhing response incubation period (s)	Turn round the body number of times	Dosage ZHENGLUOWAN group rotundin group in the low dose of Health pill of dosage medicine high dose of the present invention Health pill in the low dose of medicine of the present invention of distilled water control group medicine of the present invention	24 25 26 25 25 24 12 13	Equal-volume distilled water 1.85 3.70 5.55 1.85 3.70 0.225 0.015	278±110 254±55 270±76 276±126 294±88 280±129 266±92 282±54	28.8±16.0 27.5±13.6 23.5±13.5 19.9±14.1 ^* 23.9±13.4 21.3±13.8 14.8±12.9 ^ 6.6±4.8 ^

Compare with the distilled water matched group: ^*P＜0.05; ^*P＜0.01

2.3 the spasmolysis of medicine of the present invention test ^[3]

Get healthy new zealand rabbit (or Cavia porcellus), the mallet portion of tapping the head causes death, and takes out rabbit ileum and guinea pig ileum immediately and places tyrode's solution, wash out the enteric cavity content with tyrode's solution, get the intestinal tube that is about 2.0cm, hang in the maxwell bath pipe that fills the 20mL tyrode's solution, 37 ± 0.5 ℃ of constant temperature, bubbling air.Trace curve movement with WXT～dynamic balancing monitor, treat to add behind the intestinal tube motion stabilization being subjected to the reagent thing, the variation of record administration anterioposterior curve, and calculate the antagonism percentage rate.Observe medicine of the present invention, the antagonism the when supernatant in the Health pill aqueous solution causes that to acetylcholine tetanic property contraction of rabbit ileum and histamine cause the contraction of the tetanic property of guinea pig in vitro small intestinal of various dose respectively.

The result shows: the rabbit that medicine of the present invention causes the acetylcholine tetanic property of the ileum contraction of exsomatizing has certain antagonism, but action intensity a little less than, the Health pill effect is not obvious.Medicine of the present invention causes that to histamine the spastic contraction of guinea pig ileum has significant antagonism (table 3).

The stripped tetanic property of the ileum contraction of rabbit that table 3, medicine of the present invention cause acetylcholine

And the effect of the spastic contraction of guinea pig ileum that causes of histamine

Drug level (mg/mL)	The antagonism percentage rate (%, x ± s)
	The antagonism percentage rate (%, x ± s)		Anti-acetylcholine	Antihistaminic
	0.0 2.0 4.0 8.0	1.5±4.2(6) 21.1±7.1(6) 28.9±7.4(6) 47.2±17.5(6)	Anti-acetylcholine	Antihistaminic	0.8±3.6(6) 29.2±5.4(7) 62.9±18.0(7) 83.7±13.9(7)

Annotate: in the bracket is the intestinal segment number

3. brief summary

3.1 the test of mouse anti diarrhoea shows: three dosage of medicine of the present invention and two dosage of Health pill cause the diarrhea of mouse model to Folium Sennae obvious diarrhea effect, show as administration group 6 hours accumulative total and rush down down just number of times, in the medicine wherein of the present invention, low dose of (3.70g/kg, 1.85g/kg) and Health pill low dose (1.85g/kg) after giving the agent of Folium Sennae diarrhea inducing second 2 hours and the 3rd rushed down down just in 2 hours that number of times also obviously reduces (P＜0.05 or P＜0.01) than matched group than the obvious minimizing of matched group (P＜0.05 or P＜0.01).

3.2 the test of acetic acid twisting method shows, each dosage group of medicine of the present invention and Health pill and matched group are relatively, the equal no significant difference incubation period of writhing response (P＞0.05), medicine high dose of the present invention (5.55g/kg) group is turned round the body number of times and is obviously reduced (P＜0.05) than matched group, two dosage groups of Health pill also have certain minimizing, but difference not significantly (P＞0.05).Above results suggest medicine of the present invention and Health pill have certain analgesic activity, but onset is slow and effect a little less than.

3.3 isolated rabbit small intestinal and the test of stripped small intestine of guinea pig spasmolysis show that medicine doses of the present invention causes that to acecoline the spastic contraction of isolated rabbit ileum has certain antagonism.The spastic contraction of guinea pig ileum that histamine phosphate is caused has tangible antagonism, thinks that mainly the tonicity of dosing metagaster obviously descends, and points out medicine of the present invention that certain spasmolysis is arranged.The Health pill spasmolysis of same dose is not obvious.

List of references:

1. open bright, etc. Pericarpium Zanthoxyli antalgic and warming middle-JIAO to stop diarrhea pharmacological research, Chinese crude drug, 1994; 17 (2): 37

2. Qi Chen edits. herbal pharmacology research methodology, Beijing: People's Health Publisher, 1993 (front pages): 377～378

3. Xu Shuyun edits. pharmacological experimental methodology, Beijing: People's Health Publisher, 1982 (front pages): 865

Three of pharmacodynamic study: medicine Chinese People's Anti-Japanese Military and Political College's Mus acute gastric mucosal lesion of the present invention and to the influence of rat tolerance secretion

1 experiment material

1.1 medicine and main agents

1.1.1 drug powder of the present invention: do not contain excipient, the suitable crude drug amount of every gram 4.1g is provided lot number: 20030212 by Wanglaoji Pharmaceutical Co., Ltd., Guangzhou City.The dosage of rat is respectively 0.925g/kg, 1.85g/kg, 3.70g/kg by 5 times, 10 times, 20 times designs of clinical adult's consumption, and medicine is made into 92.5L, 185g/L, 370g/L with distilled water, and the administration volume is 10mL/kg, adopts gastric infusion.

1.1.2 BAOJIWAN: dry medicated powder (not containing excipient) is provided lot number: 20030212 by Yangcheng Medicine Co., Ltd., Guangzhou City.The dosage of mice is by 10 times of designs of clinical adult (60kg) consumption per day (0.185g/kg), and dosage is: 1.85g/kg.Medicine is made into 92.5g/L with distilled water, and the administration volume is 20mL/kg, adopts gastric infusion.

1.1.3 Selbex capsules: contain teprenone 50mg in one, make by Japanese Eisai Co., Ltd, defend material (China) company limited packing, lot number: 020581, lot number of the repackaged products: 020581A, adult consumption 150mg every day is equivalent to 2.5mg/kg, and rat dosage is by the 10 times designs of Coming-of-Age Day with dosage, be equivalent to 25mg/kg, medicine is made into 2.5g/L with distilled water, and the administration volume is the 10mL/kg body weight, adopts gastric infusion.

1.1.4 histamine phosphate: biochemical reagents, Shanghai Inst. of Biochemistry, Chinese Academy of Sciences produces, lot number: 200210291.The rat consumption is 4 μ g/kg, adopts drug administration by injection on the skin.

1.2 animal: the SD rat, 195～240g, the cleaning level, the male and female dual-purpose is provided by Guangdong Medical Lab Animal Center, the quality certification: 2002A024.

2 experimental techniques and result

2.1 medicine of the present invention Chinese People's Anti-Japanese Military and Political College Mus acute gastric mucosal lesion effect ^[1]

Get above-mentioned specification rat, be divided into 6 groups at random: the distilled water matched group, medicine of the present invention is little, in, heavy dose of group, Health pill group, histamine phosphate's group.The administration treated animal was by above-mentioned dosage administration in advance 3 days, every day 1 time, matched group is given the equal-volume distilled water, fasting is 24 hours before the last administration, after the administration 1 hour, every rat oral gavage ethanol solution hydrochloride (100mL solution contains dehydrated alcohol 60mL, HCl 15mmol) 0.7mL put to death animal after 1 hour, get stomach ligation pylorus and cardia, inject 1% formalin 10mL to gastric, stomach is dipped in in the concentration formalin 10 minutes, cut off along greater gastric curvature, be tiled on the blank sheet of paper, perusal gastric mucosa injury situation as damage index, is damaged incidence rate with the number of animals that damage takes place and the percentage calculation of animal sum with the total length (mm) of streak damage.The significant difference that compares administration group and matched group.

The results are shown in Table 1, data show in the table, and medicine of the present invention and Health pill group gastric mucosa injury index all obviously reduce than the distilled water group, and pointing out medicine of the present invention and Health pill that acidic alcohol is caused the gastric mucosa of rat acute injury has significant protective effect.

Table 1, medicine of the present invention are to the effect of rat acute gastric mucosa injury

Group	Number of animals (n)	Dosage (g/kg)	Damage incidence rate (%)	Damage index (x ± s)
Group	Number of animals (n)	Dosage (g/kg)	Damage incidence rate (%)	Damage index (x ± s)	The heavy dose of Health pill selbex of dosage in the distilled water group medicine low dose of the present invention	12 12 12 12 12 12	Equal-volume distilled water 0.925 1.85 3.70 1.85 0.025	100 75 75 75 82 70	33.0±25.9 8.3±11.1 ^* 10.9±16.1 ^* 7.5±12.9 ^ 6.2±9.6 ^ 5.3±4.9 ^**

Compare with the distilled water group: ^*P＜0.05; ^*P＜0.01

2.2 medicine of the present invention is to the influence of rat tolerance secretion ^[2]

Get above-mentioned specification rat stratified random and be divided into 6 groups: the distilled water group, medicine of the present invention is little, in, heavy dose of group, Health pill group, histamine phosphate's group.Chinese drug-treated group administration in advance 3 days, administration every day 1 time, last adopts duodenal administration.Matched group is given the equal-volume distilled water, and histamine phosphate's group adopts disposable administration.Before the last administration, animal fasting 24 hours.Etherization, routine disinfection, laparotomy ventrotomy ligation pylorus is subjected to the reagent thing from the duodenum injection, and matched group injects distilled water group, histamine phosphate's group subcutaneous injection histamine phosphate.Sew up wound, send back in the cage, put to death animal after 5 hours, collect gastric juice, with 2000rpm centrifugal 10 minutes, measure gastric juice amount (mL), get supernatant 2mL, add and start going to a nursery not and phenolphthalein indicator,, and calculate total acid output (total acidity * per hour gastric juice amount) with 0.02N NaOH titration free acid and total acidity.Adopt the t check to compare the statistical significance of each administration group and matched group.

The results are shown in Table 2, the data in the table show that medicine low dose of the present invention does not have tangible influence to rat stomach liquid measure, free acid, total acidity, total acid output, and heavy dose of gastric juice amount, total acid output then obviously reduce, and point out the inhibitory action of this dosage to stomachial secretion.The Health pill effect is not obvious.

Table 2, medicine of the present invention are to the excretory influence of rat stomach

Group	Number of animals (n)	Dosage (g/kg)	Gastric juice amount (mL)	Free acid (mmol/L)	Total acidity (mmol/L)	Total acid output (μ mol/h)
Group	Number of animals (n)	Dosage (g/kg)	Gastric juice amount (mL)	Free acid (mmol/L)	Total acidity (mmol/L)	Total acid output (μ mol/h)	The big Health pill selbex of medicine of the present invention in the distilled water group medicine small capital of the present invention invention medicine	12 12 12 12 12 10	Equal-volume distilled water 0.925 1.85 3.70 1.85 0.025	7.9±2.8 7.8±2.7 8.2±3.2 4.7±1.9 ^ 6.7±3.6 11.0±2.4 ^	69.6±25.1 78.7±29.8 8.03±22.6 80.2±26.8 70.7±28.9 83.1±6.6	105.6±18.0 82.2±37.2 102.0±15.9 101.3±26.6 101.2±21.9 116.3±5.0	169.9±70.8 168.7±84.1 181.0±83.0 109.6±51.2 ^* 133.2±92.4 253.9±62.1 ^**

Compare with the distilled water group: ^*P＜0.05; ^*P＜0.01

3 brief summaries

3.1 Chinese People's Anti-Japanese Military and Political College's Mus acute gastric mucosal lesion test shows that three dosage of medicine of the present invention (0.925g/kg, 1.85g/kg, 3.70g/kg) and Health pill (1.85g/kg) can obviously reduce the damage index that acidic alcohol causes the rat acute gastric mucosa injury.Point out two kinds of preparations that the acute gastric mucosal lesion that some foreign substance causes is had protective effect.

3.2 gastric acid secretion test shows, in in the medicine of the present invention, low dose of (1.85g/kg, 0.925g/kg) do not have tangible influence to the pylorus ligation rat tolerance secretion, heavy dose of (3.7g/kg) shows as gastric secretion and total acid output obviously reduces than matched group, points out this dosage that stomachial secretion is had inhibitory action.Health pill (1.85g/kg) does not have tangible influence to stomachial secretion.

List of references

1. your person of outstanding talent of king, Wang Jianhua, Fu Dingzhong, etc. anti-gastric mucosa injury of BUZHONG YIQI TANG and mechanism, Pharmacology and Clinics of Chinese Materia Medica, 1993; 9 (5): 5

The Qi Chen chief editor. herbal pharmacology research methodology, People's Health Publisher, 1993:859

Further set forth the preparation method of medicine of the present invention by the following examples.

Embodiment 1:

A. get prescription one: Ramulus Uncariae Cum Uncis 15.50g, Cortex Magnoliae Officinalis 80.40g, Radix Trichosanthis 55.50g, Poria 155.60g, Radix Angelicae Dahuricae 80.40g, Guangdong Massa Medicata Fermentata 80.40g, Fructus Tribuli 15.50g, Rhizoma Atractylodis 80.40g, Radix Puerariae 80.40g, Exocarpium Citri Grandis 36.70g, Fructus Oryzae Germinatus 55.50g, Flos Chrysanthemi 36.70g, Radix Aucklandiae 80.40g, Herba Pogostemonis 80.40g, Herba Menthae 36.70g, Semen Coicis 95.20g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil dextrin embedding gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water, the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction, merge extractive liquid,, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount; Merge above-mentioned concentrated solution, drying is pulverized, and gets the dry extract fine powder;

C. with dry extract fine powder and dextrin embedding thing mix homogeneously, add crospovidone, mixing is granulated drying, granulate; Promptly get granule.

Embodiment 2:

A. get prescription two: Ramulus Uncariae Cum Uncis 55.80g, Cortex Magnoliae Officinalis 215.50g, Radix Trichosanthis 205.80g, Poria 423.20g, Radix Angelicae Dahuricae 215.50g, Guangdong Massa Medicata Fermentata 215.50g, Fructus Tribuli 55.80g, Rhizoma Atractylodis 215.50g, Radix Puerariae 215.50g, Exocarpium Citri Grandis 125.40g, Fructus Oryzae Germinatus 205.80g, Flos Chrysanthemi 125.40g, Radix Aucklandiae 215.50g, Herba Pogostemonis 215.50g, Herba Menthae 125.40g, Semen Coicis 324.80g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil dextrin embedding gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water, the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated into an amount of drying, pulverizes, and gets the dry extract fine powder;

C. with dry extract fine powder and dextrin embedding thing mix homogeneously, add cane sugar powder, mixing is granulated, and promptly gets finished granule.

Embodiment 3:

A. get prescription three: Ramulus Uncariae Cum Uncis 15.50g, Cortex Magnoliae Officinalis 215.50g, Radix Trichosanthis 55.50g, Poria 155.60g, Radix Angelicae Dahuricae 215.50g, Guangdong Massa Medicata Fermentata 215.50g, Fructus Tribuli 55.80g, Rhizoma Atractylodis 215.50g, Radix Puerariae 215.50g, Exocarpium Citri Grandis 125.40g, Fructus Oryzae Germinatus 205.80g, Flos Chrysanthemi 125.40g, Radix Aucklandiae 215.50g, Herba Pogostemonis 215.50g, Herba Menthae 125.40g, Semen Coicis 95.20g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil betacyclodextrin embedding gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water, the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction, merge extractive liquid,, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount; Merge above-mentioned concentrated solution, drying is pulverized, and gets the dry extract fine powder;

C. with dry extract fine powder and dextrin embedding thing mix homogeneously, add soluble starch, mixing is granulated, and drying promptly gets finished granule.

Embodiment 4:

A. get prescription four: Ramulus Uncariae Cum Uncis 41.20g, Cortex Magnoliae Officinalis 163.68g, Radix Trichosanthis 123.17g, Poria 327.85g, Radix Angelicae Dahuricae 165.56g, Guangdong Massa Medicata Fermentata 165.56g, Fructus Tribuli 41.20g, Rhizoma Atractylodis 163.68g, Radix Puerariae 163.68g, Exocarpium Citri Grandis 81.73g, Fructus Oryzae Germinatus 123.17g, Flos Chrysanthemi 81.73g, Radix Aucklandiae 163.68g, Herba Pogostemonis 163.6g, Herba Menthae 81.73g, Semen Coicis 206.10g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and the embedding of volatile oil dextrin gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 4 times, each 0.5 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 4 times, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated into an amount of drying, pulverizes, and gets the dry extract fine powder;

C. with dry extract fine powder and dextrin embedding thing mix homogeneously, add starch, mix, granulate, promptly get granule.

Embodiment 5:

A. get prescription five: Ramulus Uncariae Cum Uncis 27.17g, Cortex Magnoliae Officinalis 109.12g, Radix Trichosanthis 81.80g, Poria 217.94g, Radix Angelicae Dahuricae 109.12g, Guangdong Massa Medicata Fermentata 109.12g, Fructus Tribuli 27.17g, Rhizoma Atractylodis 109.12g, Radix Puerariae 109.12g, change Fructus Citri tangerinae 54.48g, Fructus Oryzae Germinatus 81.80g, Flos Chrysanthemi 54.48, Radix Aucklandiae 109.12g, Herba Pogostemonis 109.12g, Herba Menthae 54.48g, Semen Coicis 136.77g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and the embedding of volatile oil dextrin gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 1 time extracted 4 hours, and the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 2 times, merge extractive liquid,, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount; Merge above-mentioned concentrated solution, drying is pulverized, and gets the dry extract fine powder;

C. with concentrate, dextrin embedding thing and cane sugar powder, dextrin mixing, granulate, drying promptly gets granule.

Embodiment 6

A. get prescription six: Ramulus Uncariae Cum Uncis 34.10g, Cortex Magnoliae Officinalis 136.40g, Radix Trichosanthis 102.30g, Poria 272.80g, Radix Angelicae Dahuricae 136.40g, Guangdong Massa Medicata Fermentata 136.40g, Fructus Tribuli 34.10g, Rhizoma Atractylodis 136.40g, Radix Puerariae 136.40g, Exocarpium Citri Grandis 68.20g, Fructus Oryzae Germinatus 102.30g, Flos Chrysanthemi 68.20g, Radix Aucklandiae 136.40g, Herba Pogostemonis 136.40g, Herba Menthae 68.20g, Semen Coicis 170.50g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with a certain amount of dextrin embedding, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 3 times, each 3 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 3 times, merge extractive liquid,, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount; Merge above-mentioned concentrated solution, drying is pulverized, and gets the dry extract fine powder;

C. concentrate, dextrin embedding thing are mixed with cyclodextrin, steviosin, granulate, be drying to obtain granule.

Embodiment 7:

A. hook taking rattan 15.50g, Cortex Magnoliae Officinalis 80.40g, Radix Trichosanthis 55.50g, Poria 155.60g, Radix Angelicae Dahuricae 80.40g, Guangdong Massa Medicata Fermentata 80.40g, Fructus Tribuli 15.50g, Rhizoma Atractylodis 80.40g, Radix Puerariae 80.40g, Exocarpium Citri Grandis 36.70g, Fructus Oryzae Germinatus 55.50g, Flos Chrysanthemi 36.70g, Radix Aucklandiae 80.40g, Herba Pogostemonis 80.40g, Herba Menthae 36.70g, Semen Coicis 95.20g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil dextrin embedding gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water, the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount;

C. adjuvant concentrate, dextrin embedding thing and granule is commonly used is granulated, and promptly gets granule.

Embodiment 8

A. hook taking rattan 55.80g, Cortex Magnoliae Officinalis 215.50g, Radix Trichosanthis 205.80g, Poria 423.20g, Radix Angelicae Dahuricae 215.50g, Guangdong Massa Medicata Fermentata 215.50g, Fructus Tribuli 55.80g, Rhizoma Atractylodis 215.50g, Radix Puerariae 215.50g, Exocarpium Citri Grandis 125.40g, Fructus Oryzae Germinatus 205.80g, Flos Chrysanthemi 125.40g, Radix Aucklandiae 215.50g, Herba Pogostemonis 215.50g, Herba Menthae 125.40g, Semen Coicis 324.80g are standby;

C. concentrate, dextrin embedding thing are added cane sugar powder, mixing is granulated, and promptly gets finished granule.

Embodiment 9

A. hook taking rattan 15.50g, Cortex Magnoliae Officinalis 215.50g, Radix Trichosanthis 55.50g, Poria 155.60g, Radix Angelicae Dahuricae 215.50g, Guangdong Massa Medicata Fermentata 215.50g, Fructus Tribuli 55.80g, Rhizoma Atractylodis 215.50g, Radix Puerariae 215.50g, Exocarpium Citri Grandis 125.40g, Fructus Oryzae Germinatus 205.80g, Flos Chrysanthemi 125.40g, Radix Aucklandiae 215.50g, Herba Pogostemonis 215.50g, Herba Menthae 125.40g, Semen Coicis 95.20g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil betacyclodextrin embedding gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water, the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount;

C. with concentrate, dextrin embedding thing and soluble starch, mixing is granulated, and drying promptly gets finished granule.

Embodiment 10

A. hook taking rattan 41.20g, Cortex Magnoliae Officinalis 163.68g, Radix Trichosanthis 123.17g, Poria 327.85g, Radix Angelicae Dahuricae 165.56g, Guangdong Massa Medicata Fermentata 165.56g, Fructus Tribuli 41.20g, Rhizoma Atractylodis 163.68g, Radix Puerariae 163.68g, Exocarpium Citri Grandis 81.73g, Fructus Oryzae Germinatus 123.17g, Flos Chrysanthemi 81.73g, Radix Aucklandiae 163.68g, Herba Pogostemonis 163.6g, Herba Menthae 81.73g, Semen Coicis 206.10g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and the embedding of volatile oil dextrin gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 4 times, each 0.5 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 4 times, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount;

C. with concentrate, dextrin embedding thing and crospovidone mixing, granulate, drying, packing promptly gets finished granule.

Embodiment 11

A. hook taking rattan 27.17g, Cortex Magnoliae Officinalis 109.12g, Radix Trichosanthis 81.80g, Poria 217.94g, Radix Angelicae Dahuricae 109.12g, Guangdong Massa Medicata Fermentata 109.12g, Fructus Tribuli 27.17g, Rhizoma Atractylodis 109.12g, Radix Puerariae 109.12g, change Fructus Citri tangerinae 54.48g, Fructus Oryzae Germinatus 81.80g, Flos Chrysanthemi 54.48, Radix Aucklandiae 109.12g, Herba Pogostemonis 109.12g, Herba Menthae 54.48g, Semen Coicis 136.77g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and the embedding of volatile oil dextrin gets dextrin embedding thing, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 1 time extracted 4 hours, and the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 2 times, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount;

C. with concentrate, dextrin embedding thing and cane sugar powder, dextrin mixing, granulate, drying, packing promptly gets finished granule.

Embodiment 12

A. hook taking rattan 34.10g, Cortex Magnoliae Officinalis 136.40g, Radix Trichosanthis 102.30g, Poria 272.80g, Radix Angelicae Dahuricae 136.40g, Guangdong Massa Medicata Fermentata 136.40g, Fructus Tribuli 34.10g, Rhizoma Atractylodis 136.40g, Radix Puerariae 136.40g, Exocarpium Citri Grandis 68.20g, Fructus Oryzae Germinatus 102.30g, Flos Chrysanthemi 68.20g, Radix Aucklandiae 136.40g, Herba Pogostemonis 136.40g, Herba Menthae 68.20g, Semen Coicis 170.50g are standby;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with a certain amount of dextrin embedding, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 3 times, each 3 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 3 times, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount;

C. with concentrate, dextrin embedding thing and dextrin, mixing is granulated, and drying promptly gets granule.

Embodiment 13

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with a certain amount of dextrin embedding, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 2 times, each 2 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 2 times, merge extractive liquid, reclaims ethanol, and extracting solution is concentrated in right amount;

C. concentrate, dextrin embedding thing are mixed with cane sugar powder, dextrin, soluble starch, mixing is granulated, and drying promptly gets finished granule.

Embodiment 14

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with a certain amount of dextrin embedding, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 2 times, each hour, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ethanol is reclaimed in Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, Radix Angelicae Dahuricae ethanol extraction 1 time, and extracting solution is concentrated in right amount;

C. with concentrate, dextrin embedding thing and crospovidone mixing, granulate, drying promptly gets finished granule.

Embodiment 15

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with the dextrin embedding of 9 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 3 times of 9 times of amounts, and each 3 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 9 times of amounts, 99% ethanol extraction 3 times, and each 0.5～5 hour, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. concentrate, dextrin embedding thing are mixed with cane sugar powder, dextrin, soluble starch or crospovidone, mixing is granulated, and drying promptly gets finished granule.

Embodiment 16

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with the dextrin embedding of 7 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 5 times of amounts, and 2 hours for the first time, 1 hour for the second time, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 4 times of amounts, 30% ethanol extraction 2 times, and 5 hours for the first time, 2 hours for the second time, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. concentrate, dextrin embedding thing are mixed with cane sugar powder, dextrin, mixing is granulated, and drying promptly gets finished granule.

Embodiment 17

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with the dextrin embedding of 3 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 6 times of amounts, and each 1.5 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 5 times of amounts, 55% ethanol extraction 2 times, and each 3.5 hours, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. concentrate, dextrin embedding thing and starch are granulated, promptly get granule.

Embodiment 18

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with the dextrin embedding of 8 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 8 times of amounts, and each 2 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 8 times of amounts, 95% ethanol extraction 3 times, and each 4 hours, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. concentrate, dextrin embedding thing are granulated with an amount of polyvinylpyrrolidone, promptly get granule.

Embodiment 19

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with the dextrin embedding of 3 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 3 times of amounts, and each 1 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae extracted 0.5 hour with 3 times of amounts, 50% ethanol extraction 1 time, merge extractive liquid,, and concentrated extracting solution reclaims ethanol to an amount of;

C. concentrate, dextrin embedding thing and steviosin are granulated, promptly get granule.

Embodiment 20

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets dextrin embedding thing with the dextrin embedding of 5 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 5 times of amounts, and 2 hours for the first time, 1 hour for the second time, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 5 times of amounts, 75% ethanol extraction 2 times, and 4 hours for the first time, 2 hours for the second time, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. concentrate, dextrin embedding thing are granulated with an amount of and lactose starch, promptly get granule.

Embodiment 21

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 6 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 2 times, each 1.5 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae extracts 2 times with 80% concentration ethanol, merge extractive liquid,, reclaim ethanol and be concentrated into an amount of, merge concentrate and betacyclodextrin embedding thing, add sucrose, dextrin, 2% polyvinylpyrrolidone is made granule.

Embodiment 22

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 3 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 1 time, each 3 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae extracts 3 times with 50% concentration ethanol, merge extractive liquid,, reclaim ethanol and be concentrated in right amount merging concentrate and betacyclodextrin embedding thing, interpolation steviosin, dextrin, ethanol is made granule.

Implement 23

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 8 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata extracting in water 3 times, each 0.5 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae extracts 1 time with 95% concentration ethanol, merge extractive liquid,, reclaim ethanol and be concentrated in right amount merging concentrate and betacyclodextrin embedding thing, interpolation starch, lactose, ethanol is made granule.

Implement 24

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 8 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, the Guangdong Massa Medicata Fermentata adds 3.5 times of water gagings and extracts 3 times, each 0.5 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae extracts 1 time with 95% concentration ethanol, merge extractive liquid,, reclaim ethanol and be concentrated into an amount of, merge concentrate and betacyclodextrin embedding thing, add lactose, dextrin, 2% polyvinylpyrrolidone is made granule.

Embodiment 25

A. get prescription, it is standby with embodiment 4 to write out a prescription;

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 3.5 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, the Guangdong Massa Medicata Fermentata adds 6 times of water gagings and extracts 2 times, each 2 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae is with 3.5 times of amounts, 90% concentration ethanol is extracted 1 time, extracted 3 hours, reclaim ethanol and be concentrated into an amount of, merge concentrate and betacyclodextrin embedding thing, add lactose, dextrin, 3% starch is made granule.

Embodiment 26

A. get prescription, it is standby with embodiment 5 to write out a prescription;

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 6 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, the Guangdong Massa Medicata Fermentata adds 4 times of water gagings and extracts 2 times, each 1 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae is with 6 times of amounts, 55% concentration ethanol is extracted 2 times, 3 hours for the first time, 2 hours for the second time, merge extractive liquid,, recovery ethanol also is concentrated in right amount, merges concentrate and betacyclodextrin embedding thing, adds 1% steviosin, dextrin, starch is made granule.

Embodiment 27

A. get prescription, it is standby with embodiment 6 to write out a prescription;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets betacyclodextrin embedding thing with the betacyclodextrin embedding of 3 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 3 times of 12 times of amounts, and 3 hours for the first time, second and third time each 1 hour, the aqueous solution of merge extractive liquid, and above-mentioned collection is concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 4.5 times of amounts, 55% ethanol extraction 2 times, and 2 hours for the first time, 1 hour for the second time, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. with concentrate and betacyclodextrin embedding thing, add 2% sucrose, dextrin, starch and make granule.

Embodiment 28

A. get prescription, it is standby with embodiment 1 to write out a prescription;

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets betacyclodextrin embedding thing with the betacyclodextrin embedding of 4 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 3 times of 4 times of amounts, and 2 hours for the first time, 1 hour for the second time, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 4 times of amounts, 60% ethanol extraction 2 times, and 2 hours for the first time, 1 hour for the second time, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. with concentrate and betacyclodextrin embedding thing, add 75% ethanol, 3% polyvinylpyrrolidone, dextrin, starch and make granule.

Embodiment 29

B. wherein the Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, and volatile oil gets betacyclodextrin embedding thing with the betacyclodextrin embedding of 5 times of amounts, and the aqueous solution after distillation device is in addition collected; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 5 times of amounts, and 2 hours for the first time, 1 hour for the second time, the aqueous solution of merge extractive liquid, and above-mentioned collection was concentrated into an amount of; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae be with 5 times of amounts, 80% ethanol extraction 2 times, and 2 hours for the first time, 1 hour for the second time, merge extractive liquid,, concentrated extracting solution reclaims ethanol to an amount of;

C. with concentrate and betacyclodextrin embedding thing, add 75% ethanol, dextrin is made granule.

Embodiment 30

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 5.5 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, the Guangdong Massa Medicata Fermentata adds 10 times of water gagings and extracts 3 times, each 2.5 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae is with 9 times of amounts, 60% concentration ethanol is extracted 2 times, each 1 hour, merge extractive liquid,, reclaim ethanol and be concentrated into an amount of, merge concentrate and betacyclodextrin embedding thing, add sucrose, starch, 1% polyvinylpyrrolidone is made granule.

Embodiment 31

A. get prescription, it is standby with embodiment 2 to write out a prescription;

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 7 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, the Guangdong Massa Medicata Fermentata adds 7 times of water gagings and extracts 2 times, each 3 hours, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae is with 4 times of amounts, 90% concentration ethanol is extracted 3 times, each 2 hours, merge extractive liquid,, reclaim ethanol and be concentrated into an amount of, merge concentrate and betacyclodextrin embedding thing, add steviosin, dextrin, water is made granule.

Embodiment 32

A. write out a prescription, it is standby with embodiment 3 to write out a prescription;

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 4.5 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, the Guangdong Massa Medicata Fermentata adds 6 times of water gagings and extracts 2 times, 2 hours for the first time, 1 hour for the second time, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae is with 6 times of amounts, 85% concentration ethanol is extracted 2 times, each 1 hour, merge extractive liquid, reclaims ethanol and is concentrated in right amount merging concentrate and betacyclodextrin embedding thing, add starch, lactose is made granule with ethanol.

Embodiment 33

A. write out a prescription, it is standby with embodiment 7 to write out a prescription;

B. get the recipe quantity Radix Aucklandiae, Herba Pogostemonis, Exocarpium Citri Grandis, Herba Menthae, Rhizoma Atractylodis use water vapour distillation volatile oil, volatile oil is with the betacyclodextrin embedding of 4.5 times of amounts, aqueous solution after distillation device is in addition collected, the Poria of medicinal residues and all the other recipe quantities, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, the Guangdong Massa Medicata Fermentata adds 4.5 times of water gagings and extracts 2 times, 1 hour for the first time, 2 hours for the second time, the aqueous solution of merge extractive liquid, and above-mentioned collection, be concentrated into an amount of, get the Ramulus Uncariae Cum Uncis of recipe quantity again, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae is with 5.5 times of amounts, 65% concentration ethanol is extracted 2 times, each 1 hour, merge extractive liquid, reclaims ethanol and is concentrated in right amount merging concentrate and betacyclodextrin embedding thing, add starch, lactose is that 20% ethanol is made granule with concentration.

Claims

1. one kind has and induces sweat, dry, with in Chinese medicine composition, it is characterized in that it is to be made by following weight portion proportioning crude drug: 15.50～55.80 parts of Ramulus Uncariae Cum Uncis, 80.40～215.50 parts of Cortex Magnoliae Officinalis, 55.50～205.80 parts of Radix Trichosanthis, 155.60～423.20 parts in Poria, 80.40～215.50 parts of the Radixs Angelicae Dahuricae, 80.40～215.50 parts of Guangdong Massa Medicata Fermentata, 15.50～55.80 parts of Fructus Tribulis, 80.40～215.50 parts of Rhizoma Atractylodis, 80.40～215.50 parts of Radix Puerariaes, 36.70～125.40 parts of Exocarpium Citri Grandises, 55.50～205.80 parts of Fructus Oryzae Germinatus, 36.70～125.40 parts of Flos Chrysanthemis, 80.40～215.50 parts of the Radix Aucklandiae, 80.40～215.50 parts of Herba Pogostemonis, 36.70～125.40 parts of Herba Menthaes, 95.20～324.80 parts of Semen Coiciss.

One kind induce sweat, dry and in the preparation method of Chinese medicine composition, comprise the steps:

A. get 15.50～55.80 parts of crude drug Ramulus Uncariae Cum Uncis, 80.40～215.50 parts of Cortex Magnoliae Officinalis, 55.50～205.80 parts of Radix Trichosanthis, 155.60～423.20 parts in Poria, 80.40～215.50 parts of the Radixs Angelicae Dahuricae, 80.40～215.50 parts of Guangdong Massa Medicata Fermentata, 15.50～55.80 parts of Fructus Tribulis, 80.40～215.50 parts of Rhizoma Atractylodis, 80.40～215.50 parts of Radix Puerariaes, 36.70～125.40 parts of Exocarpium Citri Grandises, 55.50～205.80 parts of Fructus Oryzae Germinatus, 36.70～125.40 parts of Flos Chrysanthemis, 80.40～215.50 parts of the Radix Aucklandiae, 80.40～215.50 parts of Herba Pogostemonis, 36.70～125.40 parts of Herba Menthaes, 95.20～324.80 parts of Semen Coiciss are standby;

3. as claimed in claim 2 induce sweat, dry and in the preparation method of Chinese medicine composition, comprise the steps:

A. get 27.17～41.20 parts of crude drug Ramulus Uncariae Cum Uncis, 109.12～163.68 parts of Cortex Magnoliae Officinalis, 81.80～123.17 parts of Radix Trichosanthis, 217.94～327.85 parts in Poria, 109.12～165.56 parts of the Radixs Angelicae Dahuricae, 109.12～165.56 parts of Guangdong Massa Medicata Fermentata, 27.17～41.20 parts of Fructus Tribulis, 109.12～163.68 parts of Rhizoma Atractylodis, 109.12～163.68 parts of Radix Puerariaes, 54.48～81.73 parts of Exocarpium Citri Grandises, 81.80～123.17 parts of Fructus Oryzae Germinatus, 54.48～81.73 parts of Flos Chrysanthemis, 109.12～163.68 parts of the Radix Aucklandiae, 109.12～163.6 parts of Herba Pogostemonis, 54.48～81.73 parts of Herba Menthaes, 136.77～206.10 parts of Semen Coiciss are standby;

4. as claimed in claim 3 induce sweat, dry and in the preparation method of Chinese medicine composition, comprise the steps:

A. it is standby to get 34.10 parts of crude drug Ramulus Uncariae Cum Uncis, 136.40 parts of Cortex Magnoliae Officinalis, 102.30 parts of Radix Trichosanthis, 272.80 parts in Poria, 136.40 parts of the Radixs Angelicae Dahuricae, 136.40 parts of Guangdong Massa Medicata Fermentata, 34.10 parts of Fructus Tribulis, 136.40 parts of Rhizoma Atractylodis, 136.40 parts of Radix Puerariaes, 68.20 parts of Exocarpium Citri Grandises, 102.30 parts of Fructus Oryzae Germinatus, 68.20 parts of Flos Chrysanthemis, 136.40 parts of the Radix Aucklandiae, 136.40 parts of Herba Pogostemonis, 68.20 parts of Herba Menthaes, 170.50 parts of Semen Coiciss;

5. as claimed in claim 2 induce sweat, dry and in the preparation method of Chinese medicine composition, it is characterized in that among the step b that volatile oil is with the dextrin embedding of 2～9 times of amounts; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae are with 30～99% ethanol extractions.

6. as claimed in claim 5 induce sweat, dry and in the preparation method of Chinese medicine composition, it is characterized in that volatile oil among the step b is with the dextrin embedding of 3～8 times of amounts; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae are with 50～95% ethanol extractions.

As induce sweat, dry as described in the claim 6 and in the preparation method of Chinese medicine composition, it is characterized in that volatile oil among the step b is with the dextrin embedding of 3～8 times of amounts; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 3～8 times of amounts, each 1～2 hour; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae were with 3～8 times of amounts, 50～95% ethanol extractions 1～3 time, each 0.5～4 hour.

8. induce sweat, dry as claim 7 and in the preparation method of Chinese medicine composition, it is characterized in that volatile oil among the step b is with the dextrin embedding of 3.5～6 times of amounts; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 3.5～6 times of amounts, each 1～2 hour; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae were with 3.5～6 times of amounts, 55～90% ethanol extractions 1～2 time, each 1～3 hour.

As induce sweat, dry as described in the claim 8 and in the preparation method of Chinese medicine composition, it is characterized in that volatile oil among the step b is with the dextrin embedding of 5.5 times of amounts; Medicinal residues and Poria, Flos Chrysanthemi, Fructus Tribuli, Fructus Oryzae Germinatus, Semen Coicis, Radix Trichosanthis, Guangdong Massa Medicata Fermentata add the water extraction 2 times of 4 times of amounts, 2 hours for the first time, 1 hour for the second time; Ramulus Uncariae Cum Uncis, Cortex Magnoliae Officinalis, Radix Puerariae, the Radix Angelicae Dahuricae were with 4 times of amounts, 70% ethanol extraction 2 times, 2 hours for the first time, 1 hour for the second time.

10. as claimed in claim 2 induce sweat, dry and in the preparation method of Chinese medicine composition, it is characterized in that the dextrin among the step b is a betacyclodextrin.

11. as claim 2～10 arbitrary described induce sweat, dry and in the preparation method of Chinese medicine composition, it is characterized in that the dry extract, the cyclodextrin inclusion compound that make among the step c mix with the adjuvant that granule is used always, make granule according to the conventional preparation method of granule.

12. as claimed in claim 11 induce sweat, dry and in the preparation method of Chinese medicine composition, it is characterized in that the dry extract, the cyclodextrin inclusion compound that make among the step c mix with the adjuvant that granule is used always, described adjuvant is the one or more combination in sucrose, steviosin, lactose, dextrin, starch, ethanol, the polyvinylpyrrolidone, makes granule.

13. as claimed in claim 12 induce sweat, dry and in the preparation method of Chinese medicine composition, it is characterized in that dry extract, cyclodextrin inclusion compound and an amount of sucrose, the starch that make among the step c adopting 20% alcohol granulation, make granule.