CN100543144C - 一种制备功能性蛋白肽的方法 - Google Patents
一种制备功能性蛋白肽的方法 Download PDFInfo
- Publication number
- CN100543144C CN100543144C CNB2007100977098A CN200710097709A CN100543144C CN 100543144 C CN100543144 C CN 100543144C CN B2007100977098 A CNB2007100977098 A CN B2007100977098A CN 200710097709 A CN200710097709 A CN 200710097709A CN 100543144 C CN100543144 C CN 100543144C
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- protein peptide
- enzyme digestion
- digestion reaction
- enzymolysis
- functional protein
- Prior art date
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 238000006243 chemical reaction Methods 0.000 claims abstract description 40
- 239000002994 raw material Substances 0.000 claims abstract description 30
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 28
- 238000003756 stirring Methods 0.000 claims abstract description 25
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- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 19
- 238000001035 drying Methods 0.000 claims abstract description 14
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 12
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229930000755 gossypol Natural products 0.000 claims abstract description 11
- 229950005277 gossypol Drugs 0.000 claims abstract description 11
- 239000002002 slurry Substances 0.000 claims abstract description 9
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 8
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 8
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 6
- 235000019198 oils Nutrition 0.000 claims abstract description 5
- 239000002904 solvent Substances 0.000 claims abstract description 4
- 238000012544 monitoring process Methods 0.000 claims abstract description 3
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- 102000035195 Peptidases Human genes 0.000 claims description 8
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- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 4
- 206010020843 Hyperthermia Diseases 0.000 claims description 4
- 230000033228 biological regulation Effects 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
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- 230000003301 hydrolyzing effect Effects 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
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- 238000001694 spray drying Methods 0.000 abstract description 6
- 239000003921 oil Substances 0.000 abstract description 4
- 239000002385 cottonseed oil Substances 0.000 abstract description 2
- 230000007423 decrease Effects 0.000 abstract description 2
- 238000002386 leaching Methods 0.000 abstract description 2
- 108010004032 Bromelains Proteins 0.000 abstract 1
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- 235000018102 proteins Nutrition 0.000 description 45
- 229920000742 Cotton Polymers 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
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- 238000001514 detection method Methods 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2007100977098A CN100543144C (zh) | 2007-04-28 | 2007-04-28 | 一种制备功能性蛋白肽的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2007100977098A CN100543144C (zh) | 2007-04-28 | 2007-04-28 | 一种制备功能性蛋白肽的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101063162A CN101063162A (zh) | 2007-10-31 |
CN100543144C true CN100543144C (zh) | 2009-09-23 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CNB2007100977098A Expired - Fee Related CN100543144C (zh) | 2007-04-28 | 2007-04-28 | 一种制备功能性蛋白肽的方法 |
Country Status (1)
Country | Link |
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CN (1) | CN100543144C (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103215310B (zh) * | 2013-03-27 | 2015-01-21 | 中棉紫光生物科技(上海)有限公司 | 用于发酵的棉籽蛋白及其制备方法和应用 |
CN106191183B (zh) * | 2015-05-04 | 2019-11-29 | 浙江海正药业股份有限公司 | 西兰花蛋白肽的制备方法及其应用 |
CN108267590B (zh) * | 2016-12-31 | 2021-05-11 | 江苏众红生物工程创药研究院有限公司 | Peg修饰蛋白的peg结合数检测方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1071815A (zh) * | 1991-10-26 | 1993-05-12 | 无锡轻工业学院 | 棉籽仁片脱棉酚的方法 |
CN1306078A (zh) * | 2000-12-11 | 2001-08-01 | 北京中棉紫光生物科技有限公司 | 一种棉籽的处理方法 |
-
2007
- 2007-04-28 CN CNB2007100977098A patent/CN100543144C/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1071815A (zh) * | 1991-10-26 | 1993-05-12 | 无锡轻工业学院 | 棉籽仁片脱棉酚的方法 |
CN1306078A (zh) * | 2000-12-11 | 2001-08-01 | 北京中棉紫光生物科技有限公司 | 一种棉籽的处理方法 |
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CN101063162A (zh) | 2007-10-31 |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: ZHONGFA MAIDIER BIOTECHNOLOGY JIANGSU CO., LTD. Free format text: FORMER OWNER: JIANGSU CHINA COTTON-UNIS COTTON INDUSTRY SCI. + TECH. CO., LTD. Effective date: 20140724 |
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C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20140724 Address after: Dafeng City, Jiangsu province 224100 Yimin Road Economic Development Zone No. 112 Patentee after: Maddie biotech Jiangsu Co., Ltd. Address before: Dafeng City, Jiangsu Province Economic Development Zone 224100 Yimin Road West first Patentee before: Jiangsu China Cotton-Unis Cotton Industry Sci. & Tech. Co., Ltd. |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090923 Termination date: 20160428 |