CN100459874C - A method for preparing feedstuff addictive from yeast - Google Patents
A method for preparing feedstuff addictive from yeast Download PDFInfo
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- CN100459874C CN100459874C CNB2005100346392A CN200510034639A CN100459874C CN 100459874 C CN100459874 C CN 100459874C CN B2005100346392 A CNB2005100346392 A CN B2005100346392A CN 200510034639 A CN200510034639 A CN 200510034639A CN 100459874 C CN100459874 C CN 100459874C
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Abstract
The feed additive preparing process with yeast emulsion, including fresh yeast emulsion and yeast emulsion compounded with dry yeast powder, is disclosed. The preparation process includes dilute sodium hydroxide solution treatment of yeast emulsion; twice enzymolysis after adding proteinase and pentaphosphodiesterase separately; centrifugally separating the enzymolyzed liquid into supernatant and dreg slurry; and separate concentration and drying of the supernatant and dreg slurry to obtain two feed additive products, including the product I containing four nucleotide monomers, AMP, GMP, CMP and UMP, in the content of 8-24 wt% and protein hydrolysate in the content of 50-60 wt%, and the product II containing beta-glucosan.
Description
Technical field
The present invention relates to a kind of feed addictive and the method that contains the feed addictive of β-dextran of from the yeast raw material, producing the mixture that contains nucleotide monomer and protolysate.
Background technology
Now disclosed technology of getting nucleotides by the saccharomycete system has multiple, discloses a kind of method of extracting nucleotides from beer waste yeast as CN1370837; CN1049521 discloses a kind of method of utilizing brewery's waste yeast to produce 5 ' nucleic acid; CN13970779 discloses the method for carrying nucleotides from monosodium glutamate waste liquid.More than these disclosed technology all belong to the fermenting and producing yeast or from brewer's yeast etc., extract nucleotides purified product, complex process, cost height.Do not see useful simple and low-cost as yet from the direct report that contains 4 nucleotide monomers (AMP, GMP, UMP, CMP), protolysate and contain the feed addictive of β-dextran of producing of yeast thalline.
Summary of the invention
The objective of the invention is to propose a kind of use two enzymes method is directly produced the feed addictive that contains protolysate (peptide, amino acid) and nucleotides and β-dextran from various yeast emulsions method.
The present invention comprises that with various yeast emulsions yeast cake emulsion or the emulsion of being allocated by dried yeast powder are raw material, contains yeast cells (dry weight) 8～12% in the raw emulsion.As follows from the technical process of yeast emulsion preparation feed addictive:
(1) raw material yeast emulsion is warming up to 90～95 ℃ rapidly and kills enzyme, reduce to room temperature then;
(2) hydro-oxidation is received solution, and making the emulsion naoh concentration is 0.25N, stirs to add hydrochloric acid after 30～60 minutes and transfer pH to 7.0～7.5, is incubated 50～60 ℃;
(3) yeast cells and the rna content in the sampling and measuring emulsion;
(4) add protease according to the yeast cells content of being measured to emulsion, the protease that is added be in the emulsion contained yeast cells weight 0.1～0.3%, in 50～60 ℃ of hydrolysis of temperature 3～6 hours, be warming up to 90～95 ℃ then rapidly and kill enzymes, stop reaction;
(5) said hydrolyzed liquid is transferred pH to 5.5～5.8 with hydrochloric acid, be incubated 63～65 ℃, add five phosphodiesterases according to the rna content of being measured to hydrolyzate again, every gram ribonucleic acid adds 2500～5000u (unit of activity), five phosphodiesterases, carry out enzymolysis, stirred 2～3 hours, and be warming up to 90～95 ℃ then rapidly and kill enzyme;
(6) above-mentioned enzymolysis liquid is gone out clear liquid and slag slurry through centrifugation, again that clear liquid and slag slurry is dry respectively, wherein the clear liquid drying must contain protolysate and mixture of ribonucleotides feed addictive, the dry feed addictive that must contain β-dextran of slag slurry.
In step of the present invention (4), the protease that is added can be the Alcalase 2.4L protease that papain, bromelain or Novo Nordisk (China) Bioisystech Co., Ltd produces, with Alcalase 2.4L protease the best.
Yeast emulsion raw material of the present invention can adopt:
(1) brewery's precipitation yeast paste is transferred pH to 8～9, obtains wet yeast through washing again, and it is deployed into the yeast emulsion that contains yeast cells (dry weight) 8～12%.
(2) geotrichum candidum squeezing piece transfers to the yeast emulsion that contains mycelium (dry weight) 8～12%.
(3) the Saccharomyces cerevisiae emulsion is deployed into the yeast emulsion that contains yeast cells (dry weight) 8～12%.
(3) the candida tropicalis emulsion is deployed into the yeast emulsion that contains yeast cells (dry weight) 8～12%.
Yeast emulsion raw material of the present invention then need not the enzyme that goes out as the emulsion that adopts the dried yeast powder allotment, all needs through being warmed up to 90～95 ℃ of enzymes that go out rapidly as adopting the yeast cake emulsion.
The present invention can directly produce the two kinds of products of feed addictive that obtain containing the feed addictive of 4 kinds of nucleotides (AMP, GMP, CMP, UMP) monomer and protolysate and contain β-dextran (the dry thing of yeast cell wall) from yeast by two enzymes method.In these two kinds of products, the former contained protolysate, peptides and amino acid content are up to 45～60%, and the content of 4 kinds of nucleotides (Amp, Gmp, Cmp, Ump) monomer also reaches 8～24%.This feedstuff additive product because of containing high nucleotides, can be used as the feed addictive of cub, poult, milk cow, fish, shrimp and other aquaculture things, and the immunocompetence of raising, acceleration cub, poult growth are arranged, and accelerates the restorative effect of ill domestic animal; Simultaneously, contained high protein hydrolysate, peptides, amino acid etc. are again fragrance adding agents in this product,, can improve the mouthfeel of feed, and increase the supply of protein together the time with nucleotides.And the feedstuff additive product of the resulting β of containing-dextran also is the good feed addictive of aquaculture things such as fish, shrimp, has the effect that promotes the crust growth.
The present invention is because its technology is simple, and production cost is very low, and the product nutritive validity is good, thereby has great application value.
The specific embodiment
Embodiment one
1, get the fermentation baker's yeast emulsion 1000ml that contains 10% dry weight, be warming up to 95 ℃ rapidly, the enzyme that goes out is reduced to room temperature;
2, add NaOH, making emulsion contain NaOH concentration is 0.25N, in more than 20 ℃, stirs 30～60 minutes;
3, measure yeast cells and rna content;
4, transfer pH to 7.5 with hydrochloric acid, be incubated 55 ℃; Add Alcalase 2.4L protease according to the yeast cells content of measuring by 0.2% of contained yeast cells weight, stir at a slow speed, enzymolysis was warming up to 95 ℃ rapidly after 4 hours, and enzyme goes out;
5, transfer pH to 5.6 with hydrochloric acid again, be incubated 65 ℃; Add 3000u five phosphodiesterases according to the rna content of measuring by every gram ribonucleic acid, stir at a slow speed, enzymolysis was warming up to 95 ℃ rapidly after 3 hours, and enzyme goes out;
6, enzymolysis liquid is centrifugal with tube centrifuge, after supernatant and slag slurry separated respectively the vacuum decompression drying must contain the feed addictive 1 of nucleotides and protein hydrolysate mixture and contain 2 two kinds of products of feedstuff additive product of β-dextran.
Embodiment two
1, yeast paste is precipitated in brewery, transfer pH to 8～9, obtain wet yeast through washing again, it is deployed into the yeast emulsion 1000ml that contains yeast cells (dry weight) 12%; Be warming up to 95 ℃ rapidly, the enzyme that goes out is reduced to room temperature;
2, add NaOH, making emulsion contain NaOH concentration is 0.25N, in more than 20 ℃, stirs 30～60 minutes;
3, measure yeast cells and rna content;
4, transfer pH to 7.0 with hydrochloric acid, be incubated 55 ℃; Add papain according to the yeast cells content of measuring by 0.1% of contained yeast cells weight, stir at a slow speed, enzymolysis was warming up to 95 ℃ rapidly after 5 hours, and enzyme goes out;
5, transfer pH to 5.8 with hydrochloric acid again, be incubated 65 ℃; Add 5000u five phosphodiesterases according to the rna content of measuring by every gram ribonucleic acid, stir at a slow speed, enzymolysis was warming up to 95 ℃ rapidly after 3 hours, and enzyme goes out;
6, enzymolysis liquid is centrifugal with tube centrifuge, after supernatant and slag slurry separated respectively the vacuum decompression drying must contain the feed addictive 1 of nucleotides and protein hydrolysate mixture and contain 2 two kinds of products of feedstuff additive product of β-dextran.
Embodiment three
1, enzyme squeezing piece is modulated into the cell suspension 1000ml that contains yeast 10% (dry weight) in vain, is warming up to 95 ℃ rapidly, and the enzyme that goes out is reduced to room temperature;
2, add NaOH, making emulsion contain NaOH concentration is 0.25N, in more than 20 ℃, stirs 30～60 minutes;
3, measure yeast cells and rna content;
4, transfer pH to 7.0 with hydrochloric acid, be incubated 55 ℃; Add bromelain according to the yeast cells content of measuring by 0.3% of contained yeast cells weight, stir at a slow speed, enzymolysis was warming up to 95 ℃ rapidly after 6 hours, and enzyme goes out;
5, transfer pH to 5.5 with hydrochloric acid again, be incubated 65 ℃; Add 2500u five phosphodiesterases according to the rna content of measuring by every gram ribonucleic acid, stir at a slow speed, enzymolysis was warming up to 95 ℃ rapidly after 3 hours, and enzyme goes out;
6, enzymolysis liquid is centrifugal with tube centrifuge, after supernatant and slag slurry separated respectively the vacuum decompression drying must contain the feed addictive 1 of nucleotides and protein hydrolysate mixture and contain 2 two kinds of products of feedstuff additive product of β-dextran.
Claims (2)
1. method for preparing feed addictive from yeast, with the yeast emulsion that contains yeast cells dry weight 8～12% is raw material, any that said yeast emulsion raw material is a beer waste yeast in the yeast emulsion that the yeast emulsion that the wet yeast allotment that washing obtains forms, yeast emulsion that the Saccharomyces cerevisiae emulsion is deployed into, yeast emulsion that geotrichum candidum squeezing piece is deployed into and candida tropicalis emulsion are deployed into, its preparation process is as follows:
(1) raw material yeast emulsion is warming up to 90～95 ℃ rapidly and kills enzyme, reduce to room temperature then;
(2) hydro-oxidation is received solution, and making the emulsion naoh concentration is 0.25N, stirs to add hydrochloric acid after 30～60 minutes and transfer pH to 7.0～7.5, is incubated 55～60 ℃;
(3) yeast cells and the rna content in the sampling and measuring emulsion;
(4) add protease according to the yeast cells content of being measured to emulsion, the protease that is added be in the emulsion contained yeast cells weight 0.1～0.3%, in 55～60 ℃ of hydrolysis of temperature 3～6 hours, be warming up to 90～95 ℃ then rapidly and kill enzymes;
(5) said hydrolyzed liquid is transferred pH to 5.5～5.8 with hydrochloric acid, be incubated 63～65 ℃, add five phosphodiesterases according to the rna content of being measured to hydrolyzate again, every gram ribonucleic acid adds 2500～5000u, five phosphodiesterases, carry out enzymolysis, stirred 2～3 hours, and be warming up to 90～95 ℃ then rapidly and kill enzyme;
(6) above-mentioned enzymolysis liquid is gone out clear liquid and slag slurry through centrifugation, again that clear liquid and slag slurry is dry respectively, wherein the clear liquid drying must contain the feed addictive of protolysate and Amp, Gmp, Cmp, four kinds of nucleotide monomer mixtures of Ump, the dry feed addictive that must contain β-dextran of slag slurry.
2. according to claim 1ly prepare the method for feed addictive, it is characterized in that the added protease of step (4) is Alcalase 2.4L protease from yeast.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100346392A CN100459874C (en) | 2005-05-20 | 2005-05-20 | A method for preparing feedstuff addictive from yeast |
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| CNB2005100346392A CN100459874C (en) | 2005-05-20 | 2005-05-20 | A method for preparing feedstuff addictive from yeast |
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| CN1864516A CN1864516A (en) | 2006-11-22 |
| CN100459874C true CN100459874C (en) | 2009-02-11 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613265B (en) * | 2012-03-31 | 2013-08-14 | 福建省麦都食品发展有限公司 | Natural yeast powder |
| CN102851335A (en) * | 2012-09-19 | 2013-01-02 | 宜兴市天石饲料有限公司 | Method for preparing feed nucleotide by waste beer yeast |
| CN102987169A (en) * | 2012-12-31 | 2013-03-27 | 南京同凯兆业生物技术有限责任公司 | Weaned piglet nucleotide feed additive |
| CN108740358B (en) * | 2018-05-17 | 2021-05-25 | 南京同凯兆业生物技术有限责任公司 | Feed additive and preparation method and application thereof |
| CN108378243B (en) * | 2018-05-17 | 2021-05-25 | 南京同凯兆业生物技术有限责任公司 | Feed additive for sows and application thereof |
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2005
- 2005-05-20 CN CNB2005100346392A patent/CN100459874C/en active Active
Non-Patent Citations (4)
| Title |
|---|
| 天然调味料--酵母抽提物的研究进展.. 晏志云等.食品与发酵工业.，第24卷第6期. 1998 |
| 天然调味料--酵母抽提物的研究进展.. 晏志云等.食品与发酵工业.，第24卷第6期. 1998 * |
| 酵母抽提物的生产以及在调味品工业上的应用研究.. 董家武等.食品工业科技.，第24卷第5期. 2003 |
| 酵母抽提物的生产以及在调味品工业上的应用研究.. 董家武等.食品工业科技.，第24卷第5期. 2003 * |
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