CN100443886C - Array light-emitting diode induced fluorescent tester - Google Patents
Array light-emitting diode induced fluorescent tester Download PDFInfo
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- CN100443886C CN100443886C CNB2005100468614A CN200510046861A CN100443886C CN 100443886 C CN100443886 C CN 100443886C CN B2005100468614 A CNB2005100468614 A CN B2005100468614A CN 200510046861 A CN200510046861 A CN 200510046861A CN 100443886 C CN100443886 C CN 100443886C
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Abstract
The invention relates to an array luminescent diode induced fluorescence detector which includes the array luminescent diode, the exciting optical fiber, the detection pool, the collecting optical fiber, the light filter, the photoelectric transformation device and the signal processing circuit. The detection pool is coupled connected with the other optical parts by the optical fiber. The fluorescence detector uses the same N luminescent diode as the exciting luminous source. The every luminous source irradiates to the detection pool by one exciting light fiber, so it has N light fiber to irradiate the detection pool. The exciting light fiber is fixed in the different locus of the same plane of the detection pool. The exciting light fiber and the collection light fiber are connected with the liquid stream directly; the liquid stream, the collection fiber and the exciting light fiber are crossed with each other. The detector can be used for the capillary electrophoresis, the liquid chromatogram and the flow injection. The ratio of the signal and the noise is improved by 1/2 comparing to the single luminescent diode.
Description
Technical field
The present invention relates to a kind of array light-emitting diode induced fluorescent tester, this detecting device can be used as the detecting device with flow analysis systems such as Capillary Electrophoresis, liquid chromatography, flow injections.
Background technology
Fluoroscopic examination has high sensitivity and strong selectivity as a kind of photoluminescence type detection technique, is widely used in trace samplings detects.Light emitting diode (LED) as a kind of excitation source of economy have energy consumption low (available powered battery), output power is stable, volume is little and extremely long mission life (＞10
5Hour) etc. advantage, be very suitable for microminiaturized fluorescence detecting system.The existing at present report that much detects about the LED induced fluorescence.In the former report, adopt single led as excitation source more.Single led not only excitation light intensity is low, and its intrinsic flicker noise, is one of overriding noise source of LED induced fluorescent tester.In addition, in report in the past, detection cell all adopts on the capillary column and detects.Its shortcoming is that the cross-sectional area of kapillary column type causes very strong optical reflection and scattering, causes very high optics background, is unfavorable for the reduction of system noise.
Summary of the invention
The object of the present invention is to provide a kind of array light-emitting diode induced fluorescent tester, solved the low and intrinsic problems such as flicker noise of single led excitation light intensity, this detecting device can be used as the detecting device with flow analysis systems such as Capillary Electrophoresis, liquid chromatography, flow injections.
Technical scheme of the present invention is:
A kind of array light-emitting diode induced fluorescent tester, this fluorescence detector mainly is made up of array light-emitting diode, excitation fiber, detection cell, collection optical fiber, optical filter, photoelectric conversion device and signal processing circuit, detection cell is by optical fiber and other optics couplings, and it is excitation source that this fluorescence detector adopts N identical light emitting diode; Each excitation source shines in the detection cell by 1 excitation fiber, and total N optical fiber shines in the detection cell simultaneously; Excitation fiber is fixed on the conplane different loci of detection cell, and excitation fiber, collection optical fiber all directly contact with liquid flow path, and liquid flow path, collection optical fiber and excitation fiber three grip intersection altogether; The fluorescence that sends after sample solution is stimulated receives by collecting optical fiber, and being transferred to through optical filter that photoelectric conversion device is converted into can be for the electric signal that detects, and electric signal amplifies back output by signal processing circuit.
Described liquid flow path is through-type structure.
Described excitation source is N, N 〉=2.
Described LED source is that same centre wavelength and optical property are identical.
The detection cell body is processed by non-metallic materials such as metal material or polyetheretherketone or Teflons.
Liquid flow path in the described detection cell and excitation fiber are collected optical fiber and this plane included angle and are 30 °≤α≤90 ° at grade.
Described excitation fiber adopts concentric plastic casing cover outside, and is fixed on the conplane different loci of detection cell by nut and cutting ferrule, and the plastic casing internal diameter is identical with the excitation fiber external diameter.
The present invention has following advantage:
1, the present invention adopts N (N 〉=2) led light source, and exciting light shines in the detection cell by N excitation fiber.Excitation fiber is fixed on the conplane different loci of detection cell, collects optical fiber and is used for phosphor collection and transmission, excites and collects optical fiber and all directly contact with liquid flow path, and liquid flow path, collection optical fiber and excitation fiber three grip intersection altogether.Wherein, liquid flow path and excitation fiber are collected optical fiber and are placed perpendicular to this plane at grade, and liquid flow path becomes through-type structure.Adopt the present invention excite light intensity increase for the N of single light source doubly, fluorescence signal is corresponding increase for original N doubly, and the N that its noise level only excites for single lamp in theory
1/2Doubly, thereby compare, adopts N positive same LED to excite and to make detection limit decline N with single led fluoroscopic examination of inducing
1/2In addition, adopt optical fiber directly to contact with moving phase, their less each other refractive index difference help reducing optical reflection and scattering, obtain lower optics background.
2, detecting device of the present invention can be used as the detecting device with flow analysis systems such as Capillary Electrophoresis, liquid chromatography, flow injection.
Description of drawings
Fig. 1 is the structural representation (N=4 among the figure) of array light-emitting diode induced fluorescent tester of the present invention.
Fig. 2 is the structural representation (N=6 among the figure) of three-dimensional windowless detection cell among the present invention.
Fig. 3 is used for the spectrogram of Flow Injection Analysis system for multi-light source LED induced fluorescent tester of the present invention.
Fig. 4 single light source of the present invention and two light source led induced fluorescent tester are used for the contrast of Flow Injection Analysis system.Wherein, A: single light source analysis of spectra; B: two light source analysis of spectra.
Among the figure, 1, light emitting diode; 2, excitation fiber; 3, detection cell; 4, collect optical fiber; 5, optical filter; 6, photoelectric conversion device (as photomultiplier PMT etc.); 7, signal processing circuit; 8, liquid flow path.
Embodiment
Shown in Fig. 1-2, array light-emitting diode induced fluorescent tester of the present invention, its architectural feature is: this fluorescence detector mainly is made up of array light-emitting diode 1, excitation fiber 2, detection cell 3, collection optical fiber, optical filter 5, photoelectric conversion device 6 and signal processing circuit 7.Detection cell is by optical fiber and other opticses (as light emitting diode, optical filter etc.) coupling, and it is that excitation source, exciting light shine in the detection cell 3 by N excitation fiber 2 that this fluorescence detector adopts N identical diode (N 〉=2).Excitation fiber 2 can adopt concentric plastic casing cover outside, and is fixed on the conplane different loci of detection cell by nut and cutting ferrule, and the plastic casing internal diameter is identical with the excitation fiber external diameter, the autocollimation of realization light path and need not optical correction.The sample fluorescence that produces that is stimulated is collected by another vertical collection optical fiber of placing 4, is transferred to electrooptical device 6, and change into can be for the electric signal that detects.Exciting, collect optical fiber 2,4 all directly contacts with liquid flow path 8.Liquid flow path 8, collection optical fiber 4 and excitation fiber 2 threes grip intersection altogether.Wherein, liquid flow path 8 and excitation fiber 2 are collected optical fiber 4 and are placed perpendicular to this plane at grade.8 one-tenth through-type structures of liquid flow path avoid light signal to be subjected to fluid stream deformation disturbance.
Detection cell body of the present invention can be processed by metal material or polyetheretherketone (PEEK) or Teflon non-metallic materials such as (Teflon).
The concrete implementation step of the present invention: the LED excitation beam is by lens or be directly coupled to excitation fiber 2; Shine then in the detection cell 3.Optical fiber 4 receives the fluorescence that sends after sample solution is stimulated, being transferred to through optical filter 5 that photoelectric conversion device 6 is converted into can be for the electric signal that detects by collecting.Photoelectric conversion device changes light signal into current signal, is converted into voltage signal and exports workstation or registering instrument to after signal processing circuit amplification, filtering, and voltage signal is noted.
The structural representation of array light-emitting diode induced fluorescent tester of the present invention (N=4 among the figure) as shown in Figure 1 is among the figure
" * " expression liquid flow path direction.As shown in Figure 2, the present invention adopts three-dimensional windowless detection cell, and four excitation fiber 2 are fixed on the same plane of detection cell, collects the plane of optical fiber 4 perpendicular to detection cell.
N of the present invention (N 〉=2) light source led induced fluorescent tester is used for Flow Injection Analysis system testing fluorescein isothiocynate.
Test condition: carrier fluid, water; Connecting pipe: quartz capillary (0.25mm i.d.); Sample: fluorescein isothiocynate; Sample size: 2 μ L; Flow velocity: 10 μ L/min.
Claims (5)
1, a kind of array light-emitting diode induced fluorescent tester, it is characterized in that: this fluorescence detector mainly is made up of array light-emitting diode (1), excitation fiber (2), detection cell (3), collection optical fiber (4), optical filter (5), photoelectric conversion device (6) and signal processing circuit (7), detection cell is by excitation fiber (2) and light emitting diode (1) coupling, detection cell is by collecting optical fiber (4) and optical filter (5) coupling, it is excitation source that this fluorescence detector adopts N identical light emitting diode, N 〉=2; Each excitation source shines in the detection cell by 1 excitation fiber, and total N optical fiber shines in the detection cell simultaneously; Excitation fiber is fixed on the conplane different loci of detection cell, excitation fiber (2), collection optical fiber (4) all directly contact with liquid flow path (8), liquid flow path (8), collection optical fiber (4) and excitation fiber (2) three grip intersection altogether, liquid flow path (8) in the detection cell (3) and excitation fiber (2) at grade, collection optical fiber and this plane included angle are 30 °≤α≤90 °; The fluorescence that sends after sample solution is stimulated receives by collecting optical fiber (4), and being transferred to through optical filter (5) that photoelectric conversion device (6) is converted into can be for the electric signal that detects, and electric signal amplifies back output by signal processing circuit (7).
2, according to the described array light-emitting diode induced fluorescent tester of claim 1, it is characterized in that: described liquid flow path (8) is through-type structure.
3, according to the described array light-emitting diode induced fluorescent tester of claim 1, it is characterized in that: described LED source is that same centre wavelength and optical property are identical.
4, according to the described array light-emitting diode induced fluorescent tester of claim 1, it is characterized in that: the detection cell body is processed by metal material or polyetheretherketone or Teflon.
5, according to the described array light-emitting diode induced fluorescent tester of claim 1, it is characterized in that: described excitation fiber (2) adopts concentric plastic casing cover outside, and be fixed on the conplane different loci of detection cell by nut and cutting ferrule, the plastic casing internal diameter is identical with the excitation fiber external diameter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100468614A CN100443886C (en) | 2005-07-12 | 2005-07-12 | Array light-emitting diode induced fluorescent tester |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100468614A CN100443886C (en) | 2005-07-12 | 2005-07-12 | Array light-emitting diode induced fluorescent tester |
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| CN1896723A CN1896723A (en) | 2007-01-17 |
| CN100443886C true CN100443886C (en) | 2008-12-17 |
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| CNB2005100468614A Expired - Fee Related CN100443886C (en) | 2005-07-12 | 2005-07-12 | Array light-emitting diode induced fluorescent tester |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614652B (en) * | 2008-06-25 | 2011-04-20 | 中国科学院大连化学物理研究所 | Self-aligned optical-fiber fluorescence detection cell and array fluorescence detection cell |
| CN104568755A (en) * | 2014-12-29 | 2015-04-29 | 苏州长光华医生物医学工程有限公司 | Optical fiber conduction device in chemical light emission measuring chamber |
| CN110487723A (en) * | 2019-08-26 | 2019-11-22 | 长春美泰仪器有限公司 | A kind of multidimensional integration spectrum detection cell device |
| CN112816431A (en) * | 2020-09-22 | 2021-05-18 | 西华师范大学 | Dual-wavelength capillary electrophoresis detection system for detecting tumor marker |
| CN112782141B (en) * | 2020-12-29 | 2023-12-19 | 中国科学院合肥物质科学研究院 | Plastic rapid classification equipment based on fluorescence method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59184844A (en) * | 1983-04-04 | 1984-10-20 | Shimadzu Corp | Photometer for fluorescent light and scattered light |
| CN2655229Y (en) * | 2003-09-03 | 2004-11-10 | 西北有色地质研究院 | Multi-channel atomic fluorescent spectrograph |
-
2005
- 2005-07-12 CN CNB2005100468614A patent/CN100443886C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59184844A (en) * | 1983-04-04 | 1984-10-20 | Shimadzu Corp | Photometer for fluorescent light and scattered light |
| CN2655229Y (en) * | 2003-09-03 | 2004-11-10 | 西北有色地质研究院 | Multi-channel atomic fluorescent spectrograph |
Non-Patent Citations (1)
| Title |
|---|
| 毛细管电泳-荧光/非接触电导组合型检测器的研制. 杨丙成，谭峰，关亚峰.分析化学，第33卷第5期. 2005 * |
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