CN201177602Y - Refraction compensate-micro-flowing analysis system fluorescent checking pool - Google Patents
Refraction compensate-micro-flowing analysis system fluorescent checking pool Download PDFInfo
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- CN201177602Y CN201177602Y CNU2008200116385U CN200820011638U CN201177602Y CN 201177602 Y CN201177602 Y CN 201177602Y CN U2008200116385 U CNU2008200116385 U CN U2008200116385U CN 200820011638 U CN200820011638 U CN 200820011638U CN 201177602 Y CN201177602 Y CN 201177602Y
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Abstract
The utility model relates to a fluorescent detection device, in particular to a fluorescent detection cell of a refraction compensation-microflow analysis system, which comprises a main body having a sealed hollow cavity arranged at the center part of the main body and filled with a refraction compensation liquid; a transparent capillary transversely penetrating through the hollow cavity and extending out of the main body at both ends to form a capillary channel; and two vertical optical fibers inserted into the hollow cavity and disposed on a plane vertical to the capillary channel, wherein one optical fiber is used as an excitation light path, and the other is used as a fluorescence collection light path; the central lines of the excitation light path, the fluorescence collection light path, and the capillary channel are vertical, conjugation-crossed and naturally aligned to each other. The fluorescent detection device has the advantages of low optical background, less extra-column effect, convenient use, etc.
Description
Technical field
The utility model relates to fluorescence detection device, particularly a kind of refractive power compensation-microfluidic analytic system fluorescence detection cell.
Background technology
Fluoroscopic examination has high sensitivity and very strong selectivity, uses very extensive in trace and trace samplings detection.As the vitals of fluorescence detecting system, the quality of detection cell performance has directly determined the quality of overall performance.Detect on the post is that maximum a kind of patterns is used in current fluoroscopic examination, this mode can reduce extra column effect to greatest extent, reduce the bands of a spectrum broadening, make also simply relatively, can use the external coating of removal quartz capillaries such as naked light, heating wire or hot sulfuric acid to make it to become detection window; Its major defect is: the capillary detection window in column type cross section (index of refraction 1.5) is excessive with the index of refraction difference of air (index of refraction 1.0), form air-quartz capillary wall, two optical interfaces of quartz capillary wall-air, excitation beam is produced bigger optical distortion, cause stronger parasitic light background and noise, reduced the sensitivity of detecting device.
The utility model content
The purpose of this utility model is to provide a kind of microfluidic analysis of fluorescence detection cell with liquid refractive power compensate function; This detection cell is applicable to micro-analysis systems such as Capillary Electrophoresis, capillary liquid chromatography, flow injections.
For achieving the above object, the technical solution adopted in the utility model is:
A kind of refractive power compensation-microfluidic analytic system fluorescence detection cell comprises the detection cell main body, in the centre of detection cell main body Packed cavity is set, and is filled with refractive power compensation liquid in the cavity;
One transparent capillary laterally is placed through in the cavity, and its two end forms capillary channel along the outside of stretching out the detection cell main body;
Article two, orthogonal optical fiber insert in the cavity and they be in the perpendicular plane of capillary channel in, wherein an optical fiber is as excitation light path, another optical fiber is as the phosphor collection light path;
Described excitation light path, phosphor collection light path capillary channel three's center line is vertically gripped the intersection nature mutually altogether and is aimed at.
Described transparent capillary is as detection window, and it is a detecting pattern on the capillary column, and the fiber end face that inserts in the cavity amplexiforms mutually with the transparent capillary wall.
Described transparent capillary and optical fiber are plugged on the detection cell main body, and their interface place is provided with fixing cutting ferrule and pressure cap; The transparent capillary and the optical fiber that are in cutting ferrule and pressure cap inside are arranged with teflon pipe outward.
Pond of the present utility model body is provided with excitation light path, phosphor collection light path and kapillary stationary conduit.Adopt kapillary as separating and sense channel; Adopt the fiber optic conduction exciting light and collect fluorescence, detection cell is by optical fiber and other optics coupling; Employing has the liquid close with the index of refraction of quartz capillary wall, and (refractive power compensation liquid is RIMF) as the couplant of kapillary optical window and optical fiber; Described pond body is made by nonmetallic materials such as metal material or macromolecules.
The utlity model has following advantage:
1. the 3 logical structures that intersect with X-Y plane of the utility model and process the 4th logical mode in the Z-direction of focus are with described kapillary light pond, excitation light path and phosphor collection light path three's the vertical mutually intersection of gripping altogether of center line; Adopt detecting pattern on the post, realize that extra column effect minimizes;
2. detecting pattern on the employing post has been avoided windowless detection cell problem, the problem includes: the problem to active biological sample absorption;
3. the optics background is low.The utility model adopts refractive power compensation liquid replaces air, as the couplant between capillary detection window and the excitation/emission optical fiber; Because refractive power compensation liquid has and capillary wall, the approximately equalised index of refraction of optical fiber, thereby eliminated the optical interface that causes by air, at inner realize " optical clear " of detection cell, reduced background stray light and noise level, improved detector sensitivity.
4. adopt optical fiber and other optics coupling, easy to use.
Description of drawings
Fig. 1 a is the front sectional structure synoptic diagram of the utility model along the centre;
Fig. 1 b is the side sectional structure synoptic diagram of the utility model along the centre;
Fig. 2 is used for the spectrogram of flow injection systematic analysis fluorescein for the utility model.
Fig. 3 is used for the electrophoresis spectrogram that capillary electrophoresis system is analyzed lysine, tryptophane and the phenylalanine of fluorescein isothiocynate (FITC) mark for the utility model.
Specific embodiments
Below in conjunction with accompanying drawing the utility model is further described.
Shown in figure Fig. 1 a and Fig. 1 b, the utility model comprises detection cell main body 1, and Packed cavity 5 is set in the centre of detection cell main body 1, is filled with refractive power compensation liquid in the cavity 5;
The utility model adopts has the couplant of the liquid close with the index of refraction of capillary wall as kapillary optical window and optical fiber, eliminates the optical interface that brings because of air; The pond body is made by the metal material material;
One transparent capillary laterally is placed through in the cavity 5, and its two end forms capillary channel 4 along the outside of stretching out detection cell main body 1;
Article two, orthogonal optical fiber insert in the cavity 5 and they be in the perpendicular plane of capillary channel 4 in, wherein an optical fiber is as excitation light path 2, another optical fiber is as phosphor collection light path 3, by optical fiber and other optics couplings; Described excitation light path 2, phosphor collection light path 3 capillary channels 4 threes' center line is vertically gripped the intersection nature mutually altogether and is aimed at.
Described transparent capillary is as detection window, and it is a detecting pattern on the capillary column, and the fiber end face that inserts in the cavity 5 amplexiforms mutually with the transparent capillary wall.
Described transparent capillary and optical fiber are plugged on the detection cell main body 1, and their interface place is provided with fixing cutting ferrule and pressure cap, and the transparent capillary and the optical fiber that are in cutting ferrule and pressure cap inside are arranged with teflon pipe outward.
During use, optical fiber is inserted by excitation light path 2, phosphor collection light path 3, kapillary inserts from capillary channel 4, at the optical fiber concentric Teflon sleeve pipe identical with kapillary termination point casing top external diameter, adopts nut and cutting ferrule to be fixed on the detection cell main body 1.Cavity 5 between capillary detection optical window and fiber end face is filled refractive power compensation liquid to reduce parasitic light.Exciting light imports by the excitation fiber in the light path, be radiated on the kapillary optical window, sample be stimulated the fluorescence that produces by the collection Optical Fiber Transmission in the phosphor collection light path 3 to photoelectric conversion device, being converted into can be for the electric signal of detection.
As shown in Figure 2, detection cell is used for flow injection system testing fluorescein.
Test condition: refractive power compensation liquid: glycerine; Carrier fluid: deionized water; Sample: fluorescein aqueous solution, concentration 10～500nmol/L; Flow velocity: 20 μ L/min; Sample size: 2 μ L.
Test result: getting signal to noise ratio (S/N ratio) is 3, and this detector of fluorescence induced by light-emitting diode detects fluorescein concentration and is limited to 4.3nM; Good at 10nM to the 500nM internal linear.
As shown in Figure 3, detection cell is used for lysine, tryptophane and the phenylalanine of capillary electrophoresis system test fluorescein isothiocynate (FITC) mark.
Test condition: refractive power compensation liquid: glycerine; Buffer solution: Na2B4O7 (20mM, pH9.2); The fused quartz kapillary: 0.075mm i.d. * 46/34cm (length overall/effectively); Gravity sample introduction: 10cm-15s.Separation voltage: 16kV.
Test result: 31 is lysine among the figure, and 32 is FITC, and 33 is tryptophane, and 34 is phenylalanine; Getting signal to noise ratio (S/N ratio) is 3, this detector of fluorescence induced by light-emitting diode to the concentration detectability of lysine, tryptophane and the phenylalanine of marked by fluorescein isothiocyanate be respectively 10,9,4.8nM.
Claims (4)
1, a kind of refractive power compensation-microfluidic analytic system fluorescence detection cell is characterized in that: comprise detection cell main body (1), in the centre of detection cell main body (1) Packed cavity (5) is set, be filled with refractive power compensation liquid in the cavity (5);
One transparent capillary laterally is placed through in the cavity (5), and its two end forms capillary channel (4) along the outside of stretching out detection cell main body (1);
Article two, orthogonal optical fiber inserts in the cavity (5) and in the plane that they are in and capillary channel (4) is perpendicular, wherein an optical fiber is as excitation light path (2), and another optical fiber is as phosphor collection light path (3);
Described excitation light path (2), phosphor collection light path (3) capillary channel (4) three's center line is vertically gripped the intersection nature mutually altogether and is aimed at.
2, according to the described fluorescence detection cell of claim 1, it is characterized in that: described transparent capillary is as detection window, and it is a detecting pattern on the capillary column, and the fiber end face that inserts in the cavity (5) amplexiforms mutually with the transparent capillary wall.
3. according to the described fluorescence detection cell of claim 1, it is characterized in that: described transparent capillary and optical fiber are plugged on the detection cell main body (1), and their interface place is provided with fixing cutting ferrule and pressure cap.
4. according to the described fluorescence detection cell of claim 3, it is characterized in that: the transparent capillary and the optical fiber that are in cutting ferrule and pressure cap inside are arranged with teflon pipe outward.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200116385U CN201177602Y (en) | 2008-03-19 | 2008-03-19 | Refraction compensate-micro-flowing analysis system fluorescent checking pool |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200116385U CN201177602Y (en) | 2008-03-19 | 2008-03-19 | Refraction compensate-micro-flowing analysis system fluorescent checking pool |
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| Publication Number | Publication Date |
|---|---|
| CN201177602Y true CN201177602Y (en) | 2009-01-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNU2008200116385U Expired - Lifetime CN201177602Y (en) | 2008-03-19 | 2008-03-19 | Refraction compensate-micro-flowing analysis system fluorescent checking pool |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104048918A (en) * | 2014-06-04 | 2014-09-17 | 徐静 | Total contact fluorescence detection pool for capillary electrophoresis |
| CN104181105A (en) * | 2013-05-23 | 2014-12-03 | 中国科学院大连化学物理研究所 | Sample tank used for observation of liquid oxygen fluorescence spectrum |
| CN106104254A (en) * | 2013-12-18 | 2016-11-09 | 汉迪恩公司 | In order to characterize the chip assembly of granule, flow chamber and flow cytometer |
| CN108226043A (en) * | 2016-12-22 | 2018-06-29 | 赛默飞世尔（上海）仪器有限公司 | For the measuring unit and measuring system of water quality monitoring |
| CN109870411A (en) * | 2017-12-04 | 2019-06-11 | 中国科学院大连化学物理研究所 | A kind of adjustable calibrating --- refractive power compensates fluorescence detection cell on column |
| CN112630285A (en) * | 2020-11-22 | 2021-04-09 | 上海应用技术大学 | Optical fiber detection device for capillary electrophoresis fluorescence analysis and use method thereof |
-
2008
- 2008-03-19 CN CNU2008200116385U patent/CN201177602Y/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104181105A (en) * | 2013-05-23 | 2014-12-03 | 中国科学院大连化学物理研究所 | Sample tank used for observation of liquid oxygen fluorescence spectrum |
| CN104181105B (en) * | 2013-05-23 | 2016-12-28 | 中国科学院大连化学物理研究所 | A kind of sample cell for observing liquid oxygen fluorescence spectrum |
| CN106104254A (en) * | 2013-12-18 | 2016-11-09 | 汉迪恩公司 | In order to characterize the chip assembly of granule, flow chamber and flow cytometer |
| CN106104254B (en) * | 2013-12-18 | 2019-06-07 | 深圳市芯凯瑞生物科技有限公司 | To characterize the chip assembly, flow chamber and flow cytometer of particle |
| CN104048918A (en) * | 2014-06-04 | 2014-09-17 | 徐静 | Total contact fluorescence detection pool for capillary electrophoresis |
| CN108226043A (en) * | 2016-12-22 | 2018-06-29 | 赛默飞世尔（上海）仪器有限公司 | For the measuring unit and measuring system of water quality monitoring |
| CN109870411A (en) * | 2017-12-04 | 2019-06-11 | 中国科学院大连化学物理研究所 | A kind of adjustable calibrating --- refractive power compensates fluorescence detection cell on column |
| CN109870411B (en) * | 2017-12-04 | 2021-07-06 | 中国科学院大连化学物理研究所 | Adjustable calibration refraction compensation column fluorescence detection pool |
| CN112630285A (en) * | 2020-11-22 | 2021-04-09 | 上海应用技术大学 | Optical fiber detection device for capillary electrophoresis fluorescence analysis and use method thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20090107 |
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| CX01 | Expiry of patent term |