CN101226149A - Light excitation component for testing micro pole analysis fluorescence - Google Patents
Light excitation component for testing micro pole analysis fluorescence Download PDFInfo
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- CN101226149A CN101226149A CNA2008100453256A CN200810045325A CN101226149A CN 101226149 A CN101226149 A CN 101226149A CN A2008100453256 A CNA2008100453256 A CN A2008100453256A CN 200810045325 A CN200810045325 A CN 200810045325A CN 101226149 A CN101226149 A CN 101226149A
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Abstract
The invention discloses a light excitation assembly of microcylinder fluorometric analysis detection, in particular to an assembly of micro-sample analyzer, belonging to the technical field of clinical pharmacology and life science or the like. The invention comprises a laser source (10), an optic fiber (30) and a separation microcylinder (40), wherein the front end of the fiber (30) is coupled with the laser source (10), while the back end is extruded into a detection window of the separation microcylinder (40), the fiber (30) is provided with a cone section (3) whose diameter is gradually reduced from front to back. The invention has the advantages of high laser optical transmission efficiency for improving detection sensitivity and accuracy, simple optical structure and small volume for easy laser source adjustment, and the application for combining microcylinder separation system as capillary electrophoresis, capillary liquid chromatography and flow injection or the like.
Description
Technical field
The present invention relates to the check and analysis instrument, particularly a kind of light excitation component that mainly is applicable in the micro-example micro pole analysis fluoroscopic examination in the fields such as clinical drug and life science.
Background technology
For adapting to the development trend of analytical instrument microminiaturization, highly sensitive detection method is the important content of analytical chemistry research always, and the sensitivity that therefore improves detecting device has become the key issue of Micro-Column Separation technical applicationization.Because the internal diameter very little (being generally tens to the hundreds of micron) of microtrabeculae, the volume of detection window is only received liters for several to hundreds of on the post, and the ultraviolet photometric detection method of routine can not satisfy the high sensitivity requirement of detection.Fluorescence detection has obtained fast-developing in recent years as a kind of novel high-sensitivity detection mode and has used widely, it is one of the highest optical detecting method of sensitivity up to now, its detectability to fluorescent material can reach the zmol order of magnitude, but used LASER Light Source is generally used Argon ion laser (488nm), he-Ne laser (594nm) in the laser induced fluorescence detector, and He-Cd laser device (325nm) uses less because of the life-span is short.Because gas laser, have that the light source volume is big, shortcomings such as energy consumption and cost height, limited applying of LIF.
Semiconductor diode laser and the detection of LED induced fluorescence have advantages such as cost is low, easy to operate and are widely adopted, particularly Xiao etc. has proposed a kind of based on post inner fiber directs excitation light induced fluorescence detection method (Electrophoresis, 2006,27 (2), 461-467; Electrophoresis, 2007,28,3105-3114; Electrophoresis, 2007,28,233-242), utilize these methods to be used for medicine and bioanalysis has obtained certain application (Journal ofChromatography A, 2006,1123,138-141 at Capillary Electrophoresis; Journal of Chromatography B, 2006,833 (2), 129-134; Journal of Chromatography B, 2007,856,245-251; Journal of Pharmaceutical and Biomedical Ahalysis.2007,45,362-366).The applicant is ZL200620034929.7 in the patent No., notification number is in the utility model patent instructions of CN2921831, a kind of efficient capillary electrophoresis apparatus is disclosed, in this efficient capillary electrophoresis apparatus, adopted the light transmitting fiber that stretches to the detection window position from detection port capillaceous, but the optical fibers that uses is a cylindrical fiber, and the efficient when existing with the excitation source coupling is lower.If particularly adopt cylindrical fiber, we can only select the external diameter optical fiber more smaller than capillary inner diameter to realize leaded light in the tubular stinger smoothly so, but too thin optical fiber is difficult and the fine coupling of light source, even behind light source, adopt microscope to focus on, spot size after the focusing also may surpass the cross-sectional area of selected optical fiber, it is incomplete that this will optically-coupled occur, thereby influence the launching efficiency of leaded light and fluorescence.Therefore, the exciting light that how to obtain greater efficiency is implemented fluoroscopic examination in the post, and stability, the sensitivity that improves instrument simultaneously but is that we need the further problem of solution.
Summary of the invention
Technical matters to be solved by this invention provides the light excitation component in a kind of micro pole analysis fluoroscopic examination, and this light excitation component not only has higher exciting light conduction efficiency, and transfers more convenient to light source.
The technical solution adopted for the present invention to solve the technical problems is: the light excitation component in the micro pole analysis fluoroscopic examination of the present invention, comprise excitation source, light transmitting fiber and separate microtrabeculae, this fibre-optic front end and excitation source coupling, its rear end then stretches to the detection window that separates microtrabeculae, it is characterized in that: described light transmitting fiber has diameter from front to back and reduces the conical section that forms gradually.
After taking technique scheme,, therefore have higher exciting light conduction efficiency, thereby help improving detection sensitivity and accuracy because the tapered light transmitting fiber of front can more effectively be coupled with excitation source; The sectional dimension of this light transmitting fiber front end is many greatly with respect to cylindrical fibre-optic sectional dimension, makes accent more convenient to light source.
As further optimization to technique scheme, can stretch to the detection window that separates microtrabeculae more easily for the rear end that makes optical fiber, to adapt to the different detection windows position of various separation microtrabeculaes better, described light transmitting fiber is convenient to also have the cylindrical section of longitudinal extension behind conical section.
The invention has the beneficial effects as follows to have higher exciting light conduction efficiency, help improving detection sensitivity and accuracy; Optical texture is simple, and volume is little, and it is convenient to transfer excitation source; Can with Micro-Column Separation system couplings such as Capillary Electrophoresis, capillary liquid chromatography and flow injection.
Description of drawings
This instructions comprises following five width of cloth accompanying drawings:
Fig. 1 is the synoptic diagram of the light excitation component in the micro pole analysis fluoroscopic examination of the present invention;
Fig. 2 is the present invention and the traditional cylindrical fiber fluorescence excitation comparison diagram to same sample under same light source different angles coupling condition;
Fig. 3 adopts the present invention to be connected light source with traditional cylindrical fiber respectively to measure Cobastab under the same concentration
2Electrophoretogram;
Fig. 4 does excitation source with LED and diode semiconductor laser instrument, when utilizing coupling of the present invention and traditional cylinder optical fiber and transmission exciting light respectively, to Cobastab
2The linearity curve comparison diagram that mensuration obtains;
Fig. 5 is to be that excitation source adopts two kinds of excitation modes to obtain both Capillary Electrophoresis comparison diagrams to same sample with LED.
Parts, toponym and pairing mark among the figure: excitation source 10, focalizer 20, light transmitting fiber 30, conical section 31, cylindrical section 32, separation microtrabeculae 40, detection window 41, phosphor collection device 50.
Embodiment
The present invention is further described below in conjunction with drawings and Examples.
With reference to Fig. 1, light excitation component in the micro pole analysis fluoroscopic examination of the present invention, comprise excitation source 10, light transmitting fiber 30 and separate microtrabeculae 40, the front end of this light transmitting fiber 30 and excitation source 10 couplings, its rear end then stretches to detection window 41 places that separate microtrabeculae 40.Described light transmitting fiber 30 has diameter from front to back and reduces the conical section 31 that forms gradually.An end and excitation source coupling at the bottom of the awl of the light transmitting fiber 30 among the present invention is bored a sharp end and is then inserted to separate in the microtrabeculae 40 and directly excite the sample fluoroscopic examination.The diameter of the awl bottom of light transmitting fiber 30 can be chosen to be 0.2～0.3mm usually, diameter with respect to common employing is the cylindrical light transmitting fiber of 0.04mm, its coupled end sectional dimension increases several times, therefore has higher exciting light conduction efficiency, and can improve sensitivity, enhancing stability significantly, and make accent more convenient to light source.
Because light transmitting fiber 30 has larger-diameter awl bottom, when being coupled, can allow more light enter into fibre-optic inside largely, even after focusing on, bigger hot spot is arranged with light source, can guarantee that also whole hot spot drops into fibre-optic awl bottom, realize big coupling efficiency.In addition, the conical section of the tapered light guides fiber that the present invention adopts has approximate " focusing function ", even cancellation focalizer 20 also can reach cylindrical light transmitting fiber and adopt the suitable fluorescence excitation effect of focalizer.
As a kind of preferred implementation of the present invention, for the rear end that makes optical fiber can stretch to the detection window 41 that separates microtrabeculae more easily, to adapt to the different detection windows position of various separation microtrabeculaes better.With reference to Fig. 1, described light transmitting fiber 30 also has the cylindrical section 32 of longitudinal extension behind conical section 31.The light path and the excitation light path of the phosphor collection device 50 of detection window 41 are perpendicular.Usually, also be provided with focalizer 20 between the front end of described light transmitting fiber 30 and the excitation source 10.
Fig. 2 is the present invention and the traditional cylindrical fiber fluorescence excitation comparison diagram to same sample under same light source different angles coupling condition, promptly be connected excitation source with the conical fiber among the present invention with cylindrical fiber respectively, on same surface level, change the incident angle (0～180 °) of excitation source, resulting fluorescence excitation adopts conical fiber (TOF) to connect the resulting fluorescence intensity of light source resulting fluorescence intensity when being coupled with traditional cylinder optical fiber (COF) and light source from far away as shown in the figure as can be seen from Figure.The incident angle of light source and shaft axis of optic fibre leaded light intensity of transfer pair conical fiber between-20～20 ° all is not much affected in addition, but bigger to the influence of traditional cylindrical fiber leaded light intensity.Conical fiber among the present invention excitation source that is coupled can not only obtain higher exciting light and make the stability of instrument be greatly improved.
Fig. 3 adopts the present invention to be connected light source with traditional cylindrical fiber respectively to measure under the same concentration (2.66 * 10
-6M) Cobastab
2Electrophoretogram.The sensitivity of the raising instrument that the conical fiber coupling light source that adopts among the present invention can be to a great extent can be obviously found out in diagram measurement result comparison.
Fig. 4 does excitation source with LED and diode semiconductor laser instrument, when utilizing conical fiber among the present invention and coupling of traditional cylinder optical fiber and transmission exciting light respectively, to Cobastab
2The comparison diagram of the linearity curve that mensuration obtains.As can be seen from the figure, no matter be to make excitation source with LED or with laser, linear gradient when adopting conical fiber coupling and transmission exciting light all is higher than the slope that adopts coupling of cylinder optical fiber and transmission exciting light gained linearity, that is to say and adopts conical fiber can obtain higher sensitivity.It can also be seen that from figure, adopt the sensitivity of conical fiber gained if make excitation source with LED, can reach with laser and do the sensitivity that excitation source adopts cylinder optical fiber gained, promptly both have suitable detection lower limit 0.8nM.
Fig. 5 is to be that excitation source adopts two kinds of excitation modes to obtain both Capillary Electrophoresis comparison diagrams to same sample with LED.Be not difficult to find with reference to this figure, conical fiber can not only strengthen the coupling efficiency of light, also have " optically focused effect " simultaneously, even the light that excitation source sends does not focus on and directly uses conical fiber and its coupling, the fluorescence excitation intensity of gained sample can reach yet and adopt cylinder optical fiber to realize the effect that optically-coupled obtains in conjunction with microcobjective by microcobjective.
Claims (4)
1. the light excitation component in the micro pole analysis fluoroscopic examination, comprise excitation source (10), light transmitting fiber (30) and separate microtrabeculae (40), front end of this light transmitting fiber (30) and excitation source (10) coupling, its rear end then stretches to the detection window that separates microtrabeculae (40), it is characterized in that: described light transmitting fiber (30) has diameter from front to back and reduces the conical section (31) that forms gradually.
2. the light excitation component in the micro pole analysis fluoroscopic examination according to claim 1, it is characterized in that: described light transmitting fiber (30) also has the cylindrical section (32) of longitudinal extension behind conical section (31).
3. as the light excitation component in the micro pole analysis fluoroscopic examination as described in the claim 2, it is characterized in that: the diameter of described conical section (31) awl bottom is 0.2～0.3mm, and the diameter of described cylindrical section (32) is 0.04～0.08mm.
4. as the light excitation component in the micro pole analysis fluoroscopic examination as described in the claim 1,2 or 3, it is characterized in that: be provided with focalizer (20) between the front end of described light transmitting fiber (30) and the excitation source (10).
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008100453256A CN101226149A (en) | 2008-01-31 | 2008-01-31 | Light excitation component for testing micro pole analysis fluorescence |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103308503A (en) * | 2013-06-06 | 2013-09-18 | 四川大学 | Separated micro-column coupling light guide fiber exciting light induction fluorescence component |
| CN107218576A (en) * | 2017-06-02 | 2017-09-29 | 华侨大学 | A kind of light funnel illuminator guide-lighting based on conical fiber |
| CN114674470A (en) * | 2022-03-21 | 2022-06-28 | 云南师范大学 | Transparent pressure detection device based on carbon quantum dots |
-
2008
- 2008-01-31 CN CNA2008100453256A patent/CN101226149A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103308503A (en) * | 2013-06-06 | 2013-09-18 | 四川大学 | Separated micro-column coupling light guide fiber exciting light induction fluorescence component |
| CN103308503B (en) * | 2013-06-06 | 2015-11-25 | 四川大学 | Be separated microtrabeculae coupling light transmitting fiber exciting light induced fluorescence assembly |
| CN107218576A (en) * | 2017-06-02 | 2017-09-29 | 华侨大学 | A kind of light funnel illuminator guide-lighting based on conical fiber |
| CN107218576B (en) * | 2017-06-02 | 2019-06-04 | 华侨大学 | A kind of light funnel lighting system based on conical fiber leaded light |
| CN114674470A (en) * | 2022-03-21 | 2022-06-28 | 云南师范大学 | Transparent pressure detection device based on carbon quantum dots |
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Open date: 20080723 |