CN202145194U - LED induced fluorescence detection device - Google Patents

LED induced fluorescence detection device Download PDF

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Publication number
CN202145194U
CN202145194U CN201120243593U CN201120243593U CN202145194U CN 202145194 U CN202145194 U CN 202145194U CN 201120243593 U CN201120243593 U CN 201120243593U CN 201120243593 U CN201120243593 U CN 201120243593U CN 202145194 U CN202145194 U CN 202145194U
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CN
China
Prior art keywords
photodetector
calibration platform
self calibration
phosphor collection
web member
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Expired - Fee Related
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CN201120243593U
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Chinese (zh)
Inventor
李晓琼
张志广
邓玉林
胡晓明
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Beijing Institute of Technology BIT
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Beijing Institute of Technology BIT
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Priority to CN201120243593U priority Critical patent/CN202145194U/en
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Publication of CN202145194U publication Critical patent/CN202145194U/en
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Expired - Fee Related legal-status Critical Current

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Abstract

The utility model relates to an LED (Light-Emitting Diode) fluorescence detection device belonging to the technical field of analytical detection. The LED induced fluorescence detection device comprises a gland, self-calibration platform, a fluorescence collecting lens, a photoelectric detector connecting element, an optical filter, a photoelectric detector and a support, wherein the bland is in threaded connection with the self-calibration platform; a through hole formed at the upper end of the self-calibration platform is in threaded connection with the fluorescence collecting lens, and U-shaped grooves formed at the upper end are used for mounting capillary tubes and optical fibers, respectively, while through holes formed at the lower end are in threaded connection with the photoelectric detector connecting element; the optical filter is mounted within the counter bore of the photoelectric detector connecting element; the photoelectric detector is mounted in a mounting groove thereof through a screw; and the centers of the fluorescence collecting lens, the optical filter and the photoelectric detector are located in the same axis. The LED induced fluorescence detection device overcomes the shortcomings such as large volume and the like of the conventional laser induced fluorescence detector; and the device can not only meet the requirements of high-accuracy centering and repeated positioning of the optical fibers and a detection channel, but also increase the number of the exciting optical fibers according to the requirements so as to improve the detection limit of the system.

Description

A kind of LED-induced fluorescence detection device
Technical field
The utility model relates to a kind of LED-induced fluorescence detection device, belongs to technical field of analysis and detection, is mainly used in trace and trace materials analyzing and testing in biology, chemistry, food and the environment.
Background technology
Fluoroscopic examination is a kind of highly sensitive detection technique commonly used, and the characteristic of utilizing analyte under the specific wavelength optical excitation, can produce fluorescence is carried out qualitative and quantitative detection to it.Wherein, laser Induced Fluorescence Technology has high sensitivity, but shortcomings such as the laser instrument cost is high, volume is big, energy consumption height to a great extent limit the application of laser Induced Fluorescence Technology.Semiconductor laser has volume and cost advantage, but its emission wavelength mainly is confined to the long-wavelength region at present, is difficult to find suitable fluorescent dye in this zone.In recent years, light emitting diode is applied to fluoroscopic examination and has caused people's attention gradually.Light emitting diode has advantages such as output power is stable, energy consumption is low, volume is little, the life-span is long; And emission wavelength selectable range broad; Be very suitable for the microminiaturized trend of instrument as a kind of new type light source, by the increasing analyzing and testing field that has been applied to.
The mode that induced fluorescence commonly used at present detects according to light path coupling between excitation light path and phosphor collection light path mainly is divided into: the coupling of Lens Coupling excitation fiber is collected, the coupling of optical fiber coupling excitation fiber is collected, Lens Coupling excites Lens Coupling collection etc.; Can be divided into conllinear type and orthogonal type dual mode again according to the phosphor collection light path design.
The utility model has designed light emitting diode as excitation source; Be applicable to that optical fiber excites the fluorescence detection device of the orthogonal type of collecting with lens; Not only can satisfy the high precision centrally aligned of optical fiber and sense channel and the demand of resetting; Also can increase the quantity of excitation fiber as required, improve the detectability of system.
Summary of the invention
The purpose of the utility model is to overcome defectives such as conventional laser-induced fluorescence detector is bulky, and a kind of portable light Emitting Diode-Induced Fluorescence pick-up unit is provided.
The utility model adopts following technical scheme: a kind of light Emitting Diode-Induced Fluorescence pick-up unit comprises gland, self calibration platform, phosphor collection lens, photodetector web member, optical filter, photodetector, support.Gland has internal thread; There is external thread self calibration platform upper end, and has two U type grooves, has two threaded holes on its central shaft, and lower end snail pit diameter is bigger than upper end snail pit; The phosphor collection lens have external thread; The photodetector web member has external thread, and the upper end has counterbore, and the lower end has mounting groove.
The external thread of the internal thread of gland and self calibration platform upper end is connected; Self calibration platform end upper screwed hole and phosphor collection lens can make phosphor collection reach maximum efficient through the distance of rotation phosphor collection lens adjustment with detection window through being threaded; The U type groove of self calibration platform upper end is used for installing kapillary and optical fiber respectively; Between self calibration platform lower end snail pit and photodetector web member through being threaded; Photodetector web member upper end counter bore hole diameters is slightly larger than the diameter of optical filter, optical filter is fixed in the counterbore of photodetector web member with optical cement; Photodetector is installed in the mounting groove of photodetector web member through screw; Support is connected with self calibration platform bottom, supports whole device; Phosphor collection lens, optical filter and photodetector center are in same axis, thereby reach best phosphor collection efficient.
Wherein, The self calibration platform is to utilize high-accuracy mechanical process equipment processed optical fiber-kapillary alignment device, and platform surface is processed with two U type grooves, and two U type grooves are orthogonal to intersect; Its diameter is complementary with kapillary and optical fiber respectively; Kapillary and optical fiber are individually fixed in the two U type grooves, and kapillary, both axis of optical fiber are orthogonal and intersect at a point, and there is a through hole this lower end; As the diaphragm of phosphor collection light path, the intersection point that kapillary, optical fiber intersect vertically is positioned on the central shaft of diaphragm.Thereby make the optical fiber bright dipping better be coupled into kapillary, can realize optical fiber and high duplication high precision alignment capillaceous.
The self calibration platform can realize that multiple light courcess, multispectral induced fluorescence detect; Can confirm the number of excitation source according to the height of fluorescence concentration of specimens; Can realize that also the multispectral of multiple fluorescence sample excites; Also can avoid the influence of external light source, make whole device need not lucifuge operation and extra lucifuge facility.
The excitation source of being selected for use is a light emitting diode, and it is long to choose random wave, any power consumption, and employed optical fiber can be multimode optical fiber or single-mode fiber, and can use the joint of all kinds, like type fiber joints such as SMA, FC, LC, SC and ST.
Principle of work: the exciting light that is got into by optical fiber connector passes to the self calibration platform, and it is detection window capillaceous that exciting light gets into detection cell, excites the fluorescent material emitting fluorescence in the kapillary; Excited fluorescent gets into the phosphor collection lens; And through optical filter elimination exciting light and collect fluorescence, fluorescence gets into photodetector and converts light signal into electric signal, through amplify, Filtering Processing; Output shows becomes chromatogram, promptly can be used for check and analysis.
Description of drawings
Fig. 1 is the utility model light Emitting Diode-Induced Fluorescence pick-up unit structural drawing.
Fig. 2 is self calibration platform structure figure.
Fig. 3 is the enlarged drawing of self calibration platform U type groove.
Wherein, 1-gland, 2-self calibration platform, 3-phosphor collection lens, 4-photodetector web member, 5-optical filter, 6-photodetector, 7-support
Embodiment
Below in conjunction with the accompanying drawing embodiment that develops simultaneously, the utility model is described in detail.
The utility model provides a kind of light Emitting Diode-Induced Fluorescence pick-up unit, comprises gland 1, self calibration platform 2, phosphor collection lens 3, photodetector web member 4, optical filter 5, photodetector 6, support 7.There is external thread self calibration platform 2 upper ends, and have two U type grooves, have two threaded holes on its central shaft, and the lower end through-hole diameter is bigger than upper end through hole; Phosphor collection lens 3 have external thread; Photodetector web member 4 has external thread, and the upper end has counterbore, and the lower end has mounting groove.
The external thread of the internal thread of gland 1 and self calibration platform 2 upper ends is connected; Self calibration platform 2 upper end snail pits and phosphor collection lens 3 can make phosphor collection reach maximum efficient through the distance of rotation phosphor collection lens 3 adjustment with detection window through being threaded; The U type groove of self calibration platform 2 upper ends is used for installing kapillary and optical fiber respectively; 4 of self calibration platform 2 lower end snail pits and photodetector web members are through being threaded; Photodetector web member 4 upper end counter bore hole diameters are slightly larger than the diameter of optical filter 5, optical filter 5 are fixed in the counterbore of photodetector web member 4 with optical cement; Photodetector 6 is installed in the mounting groove of photodetector web member 4 through screw; Support 7 is connected with self calibration platform 2 bottoms, supports whole device; Phosphor collection lens 3, optical filter 4 and photodetector 6 are centered close on the same axis, thereby reach best phosphor collection efficient.

Claims (3)

1. a light Emitting Diode-Induced Fluorescence pick-up unit is characterized in that, comprising: gland (1), self calibration platform (2), phosphor collection lens (3), photodetector web member (4), optical filter (5), photodetector (6), support (7); There is external thread self calibration platform (2) upper end, and has two U type grooves, has two threaded holes on its central shaft, and lower end snail pit diameter is bigger than upper end snail pit; Phosphor collection lens (3) have external thread; Photodetector web member (4) has external thread, and the upper end has counterbore, and the lower end has mounting groove;
The external thread of the internal thread of gland (1) and self calibration platform (2) upper end is connected; Self calibration platform (2) upper end snail pit and phosphor collection lens (3) can make phosphor collection reach maximum efficient through the distance of rotation phosphor collection lens (3) adjustment with detection window through being threaded; The U type groove of self calibration platform (2) upper end is used for installing kapillary and optical fiber respectively; Between self calibration platform (2) lower end through hole and photodetector web member (4) through being threaded; Photodetector web member (4) upper end counter bore hole diameters is slightly larger than the diameter of optical filter (5), with optical cement optical filter (5) is fixed in the counterbore of photodetector web member (4); Photodetector (6) is installed in the mounting groove of photodetector web member (4) through screw; Support (7) is connected with self calibration platform (2) bottom, supports whole device.
2. a kind of light Emitting Diode-Induced Fluorescence pick-up unit as claimed in claim 1; It is characterized in that the platform surface of self calibration platform (2) is processed with two U type grooves, two U type grooves are orthogonal to intersect; Its diameter is complementary with kapillary and optical fiber respectively; Kapillary and optical fiber are individually fixed in the two U type grooves, and kapillary, both axis of optical fiber are orthogonal and intersect at a point, and there is a through hole this lower end; As the diaphragm of phosphor collection light path, the intersection point that kapillary, optical fiber intersect vertically is positioned on the central shaft of diaphragm.
3. a kind of light Emitting Diode-Induced Fluorescence pick-up unit as claimed in claim 1 is characterized in that, phosphor collection lens (3), optical filter (4) and photodetector (6) are centered close on the same axis.
CN201120243593U 2011-07-12 2011-07-12 LED induced fluorescence detection device Expired - Fee Related CN202145194U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201120243593U CN202145194U (en) 2011-07-12 2011-07-12 LED induced fluorescence detection device

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Application Number Priority Date Filing Date Title
CN201120243593U CN202145194U (en) 2011-07-12 2011-07-12 LED induced fluorescence detection device

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CN202145194U true CN202145194U (en) 2012-02-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102279173A (en) * 2011-07-12 2011-12-14 北京理工大学 Light emitting diode induced fluorescence detection device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102279173A (en) * 2011-07-12 2011-12-14 北京理工大学 Light emitting diode induced fluorescence detection device

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C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120215

Termination date: 20120712