CN205426792U - Embedded capillary electrophoresis chip fluorescence detection system of optic fibre - Google Patents
Embedded capillary electrophoresis chip fluorescence detection system of optic fibre Download PDFInfo
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- CN205426792U CN205426792U CN201620253860.0U CN201620253860U CN205426792U CN 205426792 U CN205426792 U CN 205426792U CN 201620253860 U CN201620253860 U CN 201620253860U CN 205426792 U CN205426792 U CN 205426792U
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Abstract
The utility model discloses an embedded capillary electrophoresis chip fluorescence detection system of optic fibre comprises diode pumped solid green laser, focusing lens, the single channel capillary electrophoresis chip that excites optic fibre, glass material, receipt optic fibre, narrow band pass filter, photon counter, ARM9 embedded development platform and high voltage power supply, diode pumped solid green laser, focusing lens, the single channel capillary electrophoresis chip that excites optic fibre, glass material, receipt optic fibre, narrow band pass filter, photon counter and ARM9 embedded development platform connect gradually, and high voltage power supply is connected with single channel capillary electrophoresis chip. When the scheme is adopted, the utility model discloses compact structure, integrated level and detectivity are higher, the noise is lower, have fine market using value.
Description
Technical field
This utility model relates to analytical chemistry detection field, in particular, a kind of optical fibre embedded capillary electrophoresis chip fluorescence detecting system.
Background technology
Capillary electrophoresis chip (CapillaryElectrophoresisMicrochip) is the one of microflow controlled biochip, is widely used in the biochemical analysis fields such as protein component research, drug screening, gene diagnosis.During utilizing electrophoresis chip to carry out biochemical analysis, the relevant information such as the component of sample and content needs to be measured by detecting system.Owing in chip, the diameter of microchannel is generally 10 μm ~ 100 μm, sample feeding amount is few, thus sensitivity, resolution and the response speed etc. of detecting system are had higher requirement, and the performance of detecting system will directly determine the overall performance of chip analysis system.
At present, the detection method being applied in capillary electrophoresis chip analysis system mainly has laser-Induced Fluorescence Detection, chemiluminescence detection, Electrochemical Detection, Absorbance detection and Mass Spectrometer Method etc..Wherein, laser-Induced Fluorescence Detection has higher sensitivity, the material high to some fluorescence efficiency, even can be reached Single Molecule Detection by the technology such as photon counting, two-photon excitation.Meanwhile, it also has good selectivity, only on producing fluorescence or being produced impact by the fluorescently-labeled molecule of selectivity, can effectively eliminate the interference of other composition.Conventional common focusing laser-induced fluorescence detection system laser instrument and optical alignment device volume are huge, are mostly that the fluorescence signal received is delivered to personal computer to carry out processing and draw detection curve, it is necessary to rely on personal computer.Therefore, prior art existing defects, need to improve.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of new optical fibre embedded capillary electrophoresis chip fluorescence detecting system.
For achieving the above object, this utility model be have employed following technical scheme: a kind of optical fibre embedded capillary electrophoresis chip fluorescence detecting system, this system is made up of semiconductor pumped solid green light laser (1), condenser lens (2), excitation fiber (3), the single channel capillary electrophoresis chip (4) of glass material, reception optical fiber (5), narrow band pass filter (6), photon counter (7), ARM9 embedded development platform (8) and high voltage power supply (9)；
Described semiconductor pumped solid green light laser (1) is connected with one end of excitation fiber (3) by condenser lens (2), and the other end of described excitation fiber (3) accesses the optical-fibre channel of the single channel capillary electrophoresis chip (4) of glass material；
The optical-fibre channel of the single channel capillary electrophoresis chip (4) of glass material is accessed in one end of described reception optical fiber (5), receives fluorescence signal；The other end of described reception optical fiber (5) is connected with photon counter (7) by narrow band pass filter (6)；
Described photon counter (7) is connected with ARM9 embedded development platform (8)；Described high voltage power supply (9) is connected with single channel capillary electrophoresis chip (4).
Preferably, in described optical fibre embedded capillary electrophoresis chip fluorescence detecting system, described single channel capillary electrophoresis chip (4) includes sample cell (10), sample waste pond (11), buffer pool (12), buffering waste liquid pool (13), optical-fibre channel (14), sample intake passage (15), split tunnel (16) and detection region (17), described sample cell (10) is connected by sample intake passage (15) with sample waste pond (11), described buffer pool (12) is connected with split tunnel (16), described split tunnel (16) is connected through detection region (17) with buffering waste liquid pool (13), described optical-fibre channel (14) is arranged at the both sides of detection region (17).
Preferably, in described optical fibre embedded capillary electrophoresis chip fluorescence detecting system, the structure of described single channel capillary electrophoresis chip (4) is " ten " word configuration, described sample intake passage (15) is arranged with described split tunnel (16) square crossing, and described sample cell (10) and described sample waste pond (11) are arranged at the two ends of described sample intake passage (15)；
Described buffer pool (12) is arranged at split tunnel (16) and closes on one end of described sample cell (10) and described sample waste pond (11)；
Described buffering waste liquid pool (13) is arranged at the other end of split tunnel (16)；
Described detection region (17) is closed on described buffering waste liquid pool (13) and is arranged, and described optical-fibre channel (14) is arranged at the both sides of detection region (17).
Preferably, in described optical fibre embedded capillary electrophoresis chip fluorescence detecting system, described optical-fibre channel (14) includes 3 optical-fibre channels, and excitation fiber (3) is inserted in the side of described optical-fibre channel (14), introduces laser；The opposite side of described optical-fibre channel (14) connects reception optical fiber (5), receives fluorescence signal；Described optical-fibre channel (14) is vertical with described split tunnel (16).
Preferably, in described optical fibre embedded capillary electrophoresis chip fluorescence detecting system, the center emission wavelength of described semiconductor pumped solid green light laser (1) is 532nm, and power is 0 ~ 5mW continuously adjustabe, and the fluorescence signal peak wavelength exciting rhodamine B sample to produce is 580nm.
Preferably, in described optical fibre embedded capillary electrophoresis chip fluorescence detecting system, described narrow band pass filter (6) centre wavelength is 578nm, half-peak breadth 1 ~ 10nm, and peak transmission is 50%, in order to filter out the noise signal beyond fluorescence signal.
Preferably, in described optical fibre embedded capillary electrophoresis chip fluorescence detecting system, described high voltage power supply (9) is made up of three high-pressure modulars, the exportable continuously adjustable 0 ~ 5KV voltage of each module, output electric current can mutually switch less than 0.25mA, the both positive and negative polarity of the second road voltage in three tunnel outputs.
Provide the benefit that relative to prior art, use such scheme, this utility model utilizes the efficient combination of the devices such as light source, condenser lens, multimode fibre, optical filter, photon counter, enormously simplify light channel structure complicated in traditional capillary electrophoresis chip fluorescence signal detecting system, set up out the capillary electrophoresis chip detecting system that compact conformation, integrated level and detection sensitivity are higher, noise is relatively low, there is good market using value.
Accompanying drawing explanation
Fig. 1 is the structural representation of an embodiment of the present utility model；
Fig. 2 is the capillary electrophoresis chip structural representation of Fig. 1 embodiment of the present utility model；
Fig. 3-1 is rhodamine B sample solution electrophoretic separation detection one of schematic diagram with optimal conditions of the variable concentrations of Fig. 1 embodiment of the present utility model；
Fig. 3-2 be the variable concentrations of Fig. 1 embodiment of the present utility model rhodamine B sample solution with optimal conditions electrophoretic separation detection schematic diagram two；
Fig. 3-3 be the variable concentrations of Fig. 1 embodiment of the present utility model rhodamine B sample solution with optimal conditions electrophoretic separation detection schematic diagram three；
Fig. 3-4 be the variable concentrations of Fig. 1 embodiment of the present utility model rhodamine B sample solution with optimal conditions electrophoretic separation detection schematic diagram four；
Wherein, 1-semiconductor pumped solid green light laser；2-condenser lens；3-excitation fiber；4-single channel capillary electrophoresis chip；5-receives optical fiber；6-narrow band pass filter；7-photon counter；8-ARM9 embedded development platform；9-high voltage power supply；10-sample cell；11-sample waste pond；12-buffer pool；13-buffers waste liquid pool；14-optical-fibre channel；15-sample intake passage；16-split tunnel；17-detects region.
Detailed description of the invention
For the ease of understanding this utility model, below in conjunction with the accompanying drawings and specific embodiment, this utility model is described in detail.Accompanying drawing gives preferred embodiment of the present utility model.But, this utility model can realize in many different forms, however it is not limited to the embodiment described by this specification.On the contrary, providing the purpose of these embodiments is to make the understanding to disclosure of the present utility model more thorough comprehensively.
It should be noted that be referred to as " being fixed on " another element when element, it can be directly on another element or can also there is element placed in the middle.When an element is considered as " connection " another element, and it can be directly to another element or may be simultaneously present centering elements.Term " vertical ", " level ", "left", "right" and similar statement that this specification is used are for illustrative purposes only.
Unless otherwise defined, all of technology that this specification is used and scientific terminology are identical with belonging to the implication that those skilled in the art of the present utility model are generally understood that.This specification is intended merely to describe the purpose of specific embodiment at term used in the description of the present utility model, is not intended to limit this utility model.
As shown in Figure 1, an embodiment of the present utility model is, this optical fibre embedded capillary electrophoresis chip fluorescence detecting system, is made up of semiconductor pumped solid green light laser (1), condenser lens (2), excitation fiber (3), the single channel capillary electrophoresis chip (4) of glass material, reception optical fiber (5), narrow band pass filter (6), photon counter (7), ARM9 embedded development platform (8) and high voltage power supply (9)；Described semiconductor pumped solid green light laser (1) is connected with one end of excitation fiber (3) by condenser lens (2), and the other end of described excitation fiber (3) accesses the optical-fibre channel of the single channel capillary electrophoresis chip (4) of glass material；The optical-fibre channel of the single channel capillary electrophoresis chip (4) of glass material is accessed in one end of described reception optical fiber (5), receives fluorescence signal；The other end of described reception optical fiber (5) is connected with photon counter (7) by narrow band pass filter (6)；Described photon counter (7) is connected with ARM9 embedded development platform (8)；Described high voltage power supply (9) is connected with single channel capillary electrophoresis chip (4).
Preferably, described single channel capillary electrophoresis chip (4) includes sample cell (10), sample waste pond (11), buffer pool (12), buffering waste liquid pool (13), optical-fibre channel (14), sample intake passage (15), split tunnel (16) and detection region (17), described sample cell (10) is connected by sample intake passage (15) with sample waste pond (11), described buffer pool (12) is connected with split tunnel (16), described split tunnel (16) is connected through detection region (17) with buffering waste liquid pool (13), described optical-fibre channel (14) is arranged at the both sides of detection region (17).Preferably, the structure of described single channel capillary electrophoresis chip (4) is " ten " word configuration, described sample intake passage (15) is arranged with described split tunnel (16) square crossing, and described sample cell (10) and described sample waste pond (11) are arranged at the two ends of described sample intake passage (15)；Described buffer pool (12) is arranged at split tunnel (16) and closes on one end of described sample cell (10) and described sample waste pond (11)；Described buffering waste liquid pool (13) is arranged at the other end of split tunnel (16)；Described detection region (17) is closed on described buffering waste liquid pool (13) and is arranged, and described optical-fibre channel (14) is arranged at the both sides of detection region (17).Preferably, described optical-fibre channel (14) includes 3 optical-fibre channels, and excitation fiber (3) is inserted in the side of described optical-fibre channel (14), introduces laser；The opposite side of described optical-fibre channel (14) connects reception optical fiber (5), receives fluorescence signal；Described optical-fibre channel (14) is vertical with described split tunnel (16), so can make light source close detection region as far as possible, reduce the loss of exciting light, and the noise signal simultaneously making entrance fluorescence signal acquisition unit is the least.
Preferably, the center emission wavelength of described semiconductor pumped solid green light laser (1) is 532nm, and power is 0 ~ 5mW continuously adjustabe, and the fluorescence signal peak wavelength exciting rhodamine B sample to produce is 580nm.Preferably, described narrow band pass filter (6) centre wavelength is 578nm, half-peak breadth 1 ~ 10nm, and peak transmission is 50%, in order to filter out the noise signal beyond fluorescence signal.Preferably, described high voltage power supply (9) is made up of three high-pressure modulars, the exportable continuously adjustable 0 ~ 5KV voltage of each module, and output electric current can mutually switch less than 0.25mA, the both positive and negative polarity of the second road voltage in three tunnel outputs.
Preferably, high voltage power supply has controlled each liquid storage tank of chip (10,11,12,13) alive switching, realize the sample introduction of sample and separate, when sample separated passage (16) under the driving of high voltage electric field moves to detect region (17), rhodamine B sample is by laser excitation, the fluorescence produced is collected by the optical fiber of the other end, filtered arrival photon counter, photon counter sends the fluorescence signal detected to ARM9 embedded platform, is finally completed the process of signal and the real-time display etc. of test result.
Capillary electrophoresis chip used in system is glass material, and structure is as shown in Figure 2.Chip structure is basic " ten " word configuration, deep 60 μm in microchannel, wide about 100 μm；Sample feeding passage (15) long 10mm, split tunnel (16) long 40mm, at decussation point, the distance from detection region (17) is 30mm；Every pair of optical-fibre channel (14) is respectively 200 μm, 250 μm, 500 μm at a distance of 1mm, optical-fibre channel with the distance of split tunnel；The a diameter of 2mm of each liquid storage tank, volume is about 5 μ L.Additionally, the optical-fibre channel of 100 μm both can guarantee that optical fiber inserted smoothly, will not excessively loosen again and affect signal detection.
Every time before electrophoresis experiment, use the NaOH solution of 1mol/L, deionized water (18M Ω) and Tris-Borate-EDTA(2 × TBE, pH8.3 respectively) microchannel of buffer solution cleaning capillary electrophoresis chip.Take the electrophoresis chip cleaned up, four liquid storage tanks add appropriate tbe buffer solution (EDTA of BoricAcid and 2mmol/L of the Tris of 100mmol/L, 10mmol/L, formulated with deionized water), it is ensured that in passage, bubble-free produces.At optical-fibre channel, insert excitation fiber and receive optical fiber.Rhodamine B sample solution micropipettor is taken a small amount of instillation in sample cell, then arc-spark stand is moved to above chip, four electrodes are inserted in liquid storage tank.Unlatching high voltage power supply, to external control state, opens the power supply of laser instrument and photon counter, runs detection program.Open high voltage power supply, set sample introduction, separation voltage: in the sample introduction stage, sample migrates to sample waste pond (11) from sample cell (10,600V), the voltage of buffer pool (12) and buffering waste liquid pool (13) is respectively 400V and 600V, sample waste pond electrode ground connection；After sample introduction 30s, high voltage power supply switches, and the sample at cross junction enters split tunnel (16) from sample intake passage (15) and separates and detect, now, buffer pool applies 800V voltage, and sample cell and sample waste cell voltage are 600V, buffers waste liquid pool electrode ground connection.After electrophoretic procedures terminates every time, clean chip microchannel with deionized water immediately, in order to avoid buffer solution moisture evaporation blocking channel.
By four kinds of variable concentrations (1.0 × 10-4mol/L、1.0×10-5mol/L、1.0×10-6mol/L、1.0×10-7Mol/L) rhodamine B sample solution is injected separately into the sample cell of capillary electrophoresis chip, utilizes the detecting system set up to carry out electrophoretic separation experiment.Wherein, sample introduction voltage is set to: sample cell 600V, sample waste pond 0V, buffer pool 400V, buffers waste liquid pool 600V；Separation voltage is set to: buffer pool 800V, and sample cell, sample waste pond are 600V, buffers waste liquid pool 0V.The testing result obtained such as Fig. 3-1, Fig. 3-2, Fig. 3-3, shown in Fig. 3-4, four curves in figure represent the fluorescence signal intensity detected when four kinds of descending sample solutions of concentration are separated by electrophoresis successively, sample solution concentration is the biggest, and the fluorescence signal intensity produced that is stimulated is the strongest, and corresponding response curve peak value is the biggest.
Utilize that arm processor volume is little, low in energy consumption, addressing system flexibly and execution efficiency advantages of higher, for simplifying light path system structure further, make testing result depart from the dependence of personal computer, build the optical fibre embedded single channel capillary electrophoresis chip laser-induced fluorescence detection system with ARM9 processor as control core.System, with semiconductor pumped solid green light laser as light source, uses two multimode fibres transmit exciting light respectively and receive fluorescence signal；Utilizing built-in application program exploitation software design system to control interface, the fluorescence signal after processing is depicted as fluorescence curve on the TFT-LCD of WinCE platform.System makes full use of the efficient combination of the devices such as light source, condenser lens, multimode fibre, optical filter, photon counter, enormously simplify light channel structure complicated in traditional capillary electrophoresis chip fluorescence signal detecting system, set up out the capillary electrophoresis chip detecting system that compact conformation, integrated level and detection sensitivity are higher, noise is relatively low.
It should be noted that above-mentioned each technical characteristic continues to be mutually combined, form various embodiments the most enumerated above, be accordingly to be regarded as the scope that this utility model description is recorded；Further, for those of ordinary skills, can be improved according to the above description or be converted, and all these modifications and variations all should be belonged to the protection domain of this utility model claims.
Claims (7)
1. an optical fibre embedded capillary electrophoresis chip fluorescence detecting system, it is characterized in that, this system is made up of semiconductor pumped solid green light laser (1), condenser lens (2), excitation fiber (3), the single channel capillary electrophoresis chip (4) of glass material, reception optical fiber (5), narrow band pass filter (6), photon counter (7), ARM9 embedded development platform (8) and high voltage power supply (9)；
Described semiconductor pumped solid green light laser (1) is connected with one end of excitation fiber (3) by condenser lens (2), and the other end of described excitation fiber (3) accesses the optical-fibre channel of the single channel capillary electrophoresis chip (4) of glass material；
The optical-fibre channel of the single channel capillary electrophoresis chip (4) of glass material is accessed in one end of described reception optical fiber (5), receives fluorescence signal；The other end of described reception optical fiber (5) is connected with photon counter (7) by narrow band pass filter (6)；
Described photon counter (7) is connected with ARM9 embedded development platform (8)；Described high voltage power supply (9) is connected with single channel capillary electrophoresis chip (4).
Optical fibre embedded capillary electrophoresis chip fluorescence detecting system the most according to claim 1, it is characterized in that, described single channel capillary electrophoresis chip (4) includes sample cell (10), sample waste pond (11), buffer pool (12), buffering waste liquid pool (13), optical-fibre channel (14), sample intake passage (15), split tunnel (16) and detection region (17), described sample cell (10) is connected by sample intake passage (15) with sample waste pond (11), described buffer pool (12) is connected with split tunnel (16), described split tunnel (16) is connected through detection region (17) with buffering waste liquid pool (13), described optical-fibre channel (14) is arranged at the both sides of detection region (17).
Optical fibre embedded capillary electrophoresis chip fluorescence detecting system the most according to claim 2, it is characterized in that, the structure of described single channel capillary electrophoresis chip (4) is " ten " word configuration, described sample intake passage (15) is arranged with described split tunnel (16) square crossing, and described sample cell (10) and described sample waste pond (11) are arranged at the two ends of described sample intake passage (15)；
Described buffer pool (12) is arranged at split tunnel (16) and closes on one end of described sample cell (10) and described sample waste pond (11)；
Described buffering waste liquid pool (13) is arranged at the other end of split tunnel (16)；
Described detection region (17) is closed on described buffering waste liquid pool (13) and is arranged, and described optical-fibre channel (14) is arranged at the both sides of detection region (17).
Optical fibre embedded capillary electrophoresis chip fluorescence detecting system the most according to claim 3, it is characterised in that described optical-fibre channel (14) includes 3 optical-fibre channels, the side of described optical-fibre channel (14) is inserted excitation fiber (3), is introduced laser；The opposite side of described optical-fibre channel (14) connects reception optical fiber (5), receives fluorescence signal；Described optical-fibre channel (14) is vertical with described split tunnel (16).
Optical fibre embedded capillary electrophoresis chip fluorescence detecting system the most according to claim 2, it is characterized in that, the center emission wavelength of described semiconductor pumped solid green light laser (1) is 532nm, power is 0 ~ 5mW continuously adjustabe, and the fluorescence signal peak wavelength exciting rhodamine B sample to produce is 580nm.
Optical fibre embedded capillary electrophoresis chip fluorescence detecting system the most according to claim 5, it is characterized in that, described narrow band pass filter (6) centre wavelength is 578nm, half-peak breadth 1 ~ 10nm, peak transmission is 50%, in order to filter out the noise signal beyond fluorescence signal.
Optical fibre embedded capillary electrophoresis chip fluorescence detecting system the most according to claim 6, it is characterized in that, described high voltage power supply (9) is made up of three high-pressure modulars, the exportable continuously adjustable 0 ~ 5KV voltage of each module, output electric current can mutually switch less than 0.25mA, the both positive and negative polarity of the second road voltage in three tunnel outputs.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112630285A (en) * | 2020-11-22 | 2021-04-09 | 上海应用技术大学 | Optical fiber detection device for capillary electrophoresis fluorescence analysis and use method thereof |
| WO2024086899A1 (en) * | 2022-10-28 | 2024-05-02 | University Of Tasmania | Electrophoretic method, devices and systems |
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2016
- 2016-03-30 CN CN201620253860.0U patent/CN205426792U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112630285A (en) * | 2020-11-22 | 2021-04-09 | 上海应用技术大学 | Optical fiber detection device for capillary electrophoresis fluorescence analysis and use method thereof |
| WO2024086899A1 (en) * | 2022-10-28 | 2024-05-02 | University Of Tasmania | Electrophoretic method, devices and systems |
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