CN100353922C - Cross breeding method among venussear mottles of different geographical population - Google Patents
Cross breeding method among venussear mottles of different geographical population Download PDFInfo
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- CN100353922C CN100353922C CNB2005100967701A CN200510096770A CN100353922C CN 100353922 C CN100353922 C CN 100353922C CN B2005100967701 A CNB2005100967701 A CN B2005100967701A CN 200510096770 A CN200510096770 A CN 200510096770A CN 100353922 C CN100353922 C CN 100353922C
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- different geographical
- haliotis diversicolor
- abalones
- colorful
- synchronous
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention discloses a hybridization method of colorful abalones from different geographical populations. The present invention relates to a method of the genetic improvement of colorful abalones, particularly to a hybridization method of colorful abalones from different geographical populations. The present invention provides a hybridization method of colorful abalones from different geographical populations. Abalones are put in sea water at the temperature of 16 to 18 DEG C, and the sea water is heated for the synchronous maturity promoting culture of parent abalones. Feed is prepared from 100 parts of gracilaria, 30 to 60 parts of ulva, 20 to 50 parts of undaria pinnitafida and 1 to 15 parts of spirulina. 5-hydroxytryptamine is injected in the head or the abdominal leg of one colorful abalone. The injected colorful abalone and a colorful abalone from another producing area are made to synchronously oviposit and ejaculate. Sperms and ova are artificially inseminated to obtain filial generations. The method can guarantee a survival rate in long-distance transport. The synchronous maturity of parent abalones from two populations is promoted by reasonable feed collocation. The injection spawning inducement of 5-hydroxytryptamine is used for the synchronous discharge of gametes. Therefore, the method solves the problems of the geographic isolation and the physiological differences of sexual maturity and gamete discharge of different geographical populations. The method can promote the synchronous maturity of gonads and the synchronous discharge of gametes.
Description
Technical field
The present invention relates to a kind of method of Haliotis diversicolor genetic improvement, particularly a kind of is the method that adopts the Haliotis diversicolor of different geographical population to hybridize.
Background technology
At present, produce Bao Miao with Taiwan colony from numerous mode in the production, the shortcoming of this production method is: inbreeding causes the character decline to cause the Bao Miao premunition poor, and survival rate is low, and frequent appearance is extensive dead.It is to improve an effective way of resistance and fertility performance that the method for employing different groups intermolecular hybrid is utilized its hybrid vigor.Pedro Cruz etc. utilizes different scallop colonies to hybridize, the result shows that juvenile stage hybridization group is all showing significant hybrid vigor (PedroCruz aspect growth and the survival two, Ana M Ibarra.Larval growth and survival of two catarina scallop (Argopectencirularis, Sowerby, 1835) population and their reciprocal crosses[J] .Journal ofExperimental Marine Biology and Ecology, 1997,212:95-110.).Domestic in shellfish, also have see similar research, as Liu Xiaolin etc. the hybridization of Chlamys farreri China population and Japanese population has been carried out reporting (Liu Xiaolin, Chang Yaqing, Xiang Jianhai, early growth and development [J] Deng Chlamys farreri China population and Japanese population first-filial generation. aquatic product journal, 2002,26 (5): 385-390.).But fail to solve the hybridization problem that adopts Haliotis diversicolor (Haliotis diversicolor) between different geographical populations for a long time, its major obstacle is a geographical distribution difference, gonad maturity season and gamete discharging differences of Physiological are not appeared in the newspapers as yet about the research that how to overcome this obstacle so far.
Summary of the invention
The present invention is intended to the shortcoming that exists in the existing Bao Miao production method, and fails for a long time to solve and adopt the problem that Haliotis diversicolor hybridizes between different geographical populations, and the hybridizing method between a kind of different geographical population Haliotis diversicolor is provided.
Hybridizing method between the said different geographical population Haliotis diversicolors of the present invention the steps include:
1), the Bao of 2-4cm specification is progressively cooled to 16～18 ℃ according to 1～3 ℃ cooling rate per hour, puts into 16～18 ℃, be preferably in 18 ℃ the sea water, progressively heat up according to 0.2～1 ℃ programming rate per hour again;
2), according to the life habit of Haliotis diversicolor, carrying out the synchronous accelerating of close Bao cultivates, its composition of the bait of throwing something and feeding and be Gracilaria tenuistipitata 100, ulva 30～60, Thallus Laminariae 20～50, spirulina 1～15 by the content of mass ratio is preferably Gracilaria tenuistipitata 100, ulva 40～50, Thallus Laminariae 30～40, spirulina 2～10;
3), at the head of every Haliotis diversicolor or the injection 0.8～1.2ml of abdominal foot portion, be preferably 1ml, concentration is (0.8～1.2) * 10
-3Mol/L is preferably 10
-3The 5-hydroxy tryptamine of mol/L, it is smart that itself and another place of production Haliotis diversicolor synchronous spawning is arranged;
4), with the ovum artificial insemination that the sperm and the female Haliotis diversicolor of the discharging of male Haliotis diversicolor are discharged, obtain filial generation.
1), can guarantee well the survival rate of Haliotis diversicolor long-distance transport can reach 90% usually compare with existing Bao Miao production method, the present invention has following outstanding effect:; 2), adopting the collocation of multiple natural bait and artitificial food to raise facilitates the parent of two colonies Bao ripe synchronously; 3), adopting the 5-hydroxy tryptamine injection to hasten parturition reaches the synchronous discharging of gamete, has overcome geographical isolation, sexual maturity and gamete discharging differences of Physiological between different geographical population, can promote the synchronous maturation and the gamete of gonad to discharge synchronously; 4), resulting filial generation is suitable with the selfing group aspect indexs such as rate of fertilization, incubation rate, adhesive rate, workable, 5), problems such as the rate of fertilization that does not exist intervarietal hybridization to occur is low, abnormal rate height: at the forming stage in later stage, the Bao Miao that the growth of this technology gained Bao Miao and survival rate all are better than traditional method for culturing seedlings gained.
The specific embodiment
Embodiment 1
In Japanese original producton location, the Bao of 2.5～3cm specification is progressively dropped to 18 ℃ of transport temperatures from normal water temperature according to 2 ℃ cooling rate per hour.After transportation reaches the destination, put into 18 ℃ sea water, progressively rise to normal water temperature according to 1 ℃ programming rate per hour again, the transportation survival rate reaches 90%.According to the life habit of Japanese Haliotis diversicolor, carry out the synchronous accelerating of close Bao and cultivate, feed according to the mixed bait of Gracilaria tenuistipitata 100, ulva 40, Thallus Laminariae 30, spirulina 8.Select the full Japanese Bao of gonad, every head or abdominal foot portion injection 1ml concentration are 10
-3The 5-hydroxy tryptamine of mol/L, row is smart after 2～3 hours.The ovum that the sperm of Japanese Haliotis diversicolor (hero) discharging and Taiwan Haliotis diversicolor (female) are discharged obtains all suitable with selfing group filial generations such as rate of fertilization, incubation rate, adhesive rate according to certain mixed.Back 8 the lunar crust length of collecting seedling are higher by 10.2% than selfing group, and the survival rate of high 13.2%, the 10 monthly age hybridization of body weight group is higher by 46.7% than selfing group.
Embodiment 2
Similar to Example 1, its difference is the Bao of 2～2.5cm specification is progressively dropped to 17 ℃ of transport temperatures from normal water temperature according to 3 ℃ cooling rate per hour.After transportation reaches the destination, put into 17 ℃ sea water, progressively rise to normal water temperature according to 0.2 ℃ programming rate per hour again.Carry out the synchronous accelerating of close Bao when cultivating, feed according to the mixed bait of Gracilaria tenuistipitata 100, ulva 30, Thallus Laminariae 50, spirulina 15.Head or abdominal foot portion injection 1ml concentration every Bao are 1.2 * 10
-3The 5-hydroxy tryptamine of mol/L, row is smart after 2～3 hours.
Embodiment 3
Similar to Example 1, its difference is the Bao of 3.5～4cm specification is progressively dropped to 16 ℃ of transport temperatures from normal water temperature according to 1 ℃ cooling rate per hour.After transportation reaches the destination, put into 16 ℃ sea water, progressively rise to normal water temperature according to 0.5 ℃ programming rate per hour again.Carry out the synchronous accelerating of close Bao when cultivating, feed according to the mixed bait of Gracilaria tenuistipitata 100, ulva 60, Thallus Laminariae 20, spirulina 1.Head or abdominal foot portion injection 0.8ml concentration every Bao are 0.8 * 10
-3The 5-hydroxy tryptamine of mol/L, row is smart after 2～3 hours.
Claims (4)
1, the hybridizing method between different geographical population Haliotis diversicolors is characterized in that the steps include:
1), the Bao of 2～4cm specification is progressively cooled to 16～18 ℃ according to 1～3 ℃ cooling rate per hour, puts into 16～18 ℃ sea water after arriving destination, progressively heat up according to 0.2～1 ℃ programming rate per hour again in the original producton location;
2), according to the life habit of Haliotis diversicolor, carry out the synchronous accelerating of close Bao and cultivate, its composition of the bait of throwing something and feeding and be Gracilaria tenuistipitata 100, ulva 30～60, Thallus Laminariae 20～50, spirulina 1～15 by the content of mass ratio;
3), at the head of every Haliotis diversicolor or the injection 0.8～1.2ml of abdominal foot portion, concentration is (0.8～1.2) * 10
-3The 5-hydroxy tryptamine of mol/L makes the female Haliotis diversicolor of destination and the male Haliotis diversicolor synchronous spawning row essence in original producton location;
4), with the ovum artificial insemination that the sperm and the female Haliotis diversicolor of destination of the male Haliotis diversicolor discharging in original producton location are discharged, obtain filial generation.
2, the hybridizing method between different geographical population Haliotis diversicolor as claimed in claim 1, it is characterized in that in step 1), the Bao of 2-4cm specification is progressively cooled to 16～18 ℃ according to 1～3 ℃ cooling rate per hour, put into 18 ℃ sea water, progressively heat up according to 0.2～1 ℃ programming rate per hour again.
3, the hybridizing method between different geographical population Haliotis diversicolor as claimed in claim 1 is characterized in that in step 2) in, its composition of the bait of throwing something and feeding and be Gracilaria tenuistipitata 100, ulva 40～50, Thallus Laminariae 30～40, spirulina 2～10 by the content of mass ratio.
4, the hybridizing method between different geographical population Haliotis diversicolor as claimed in claim 1 is characterized in that in step 3), and at head or the injection 1ml of abdominal foot portion of every Haliotis diversicolor, concentration is 10
-3The 5-hydroxy tryptamine of mol/L makes the female Haliotis diversicolor of destination and the male Haliotis diversicolor synchronous spawning row essence in original producton location.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100967701A CN100353922C (en) | 2005-09-01 | 2005-09-01 | Cross breeding method among venussear mottles of different geographical population |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100967701A CN100353922C (en) | 2005-09-01 | 2005-09-01 | Cross breeding method among venussear mottles of different geographical population |
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| CN1736344A CN1736344A (en) | 2006-02-22 |
| CN100353922C true CN100353922C (en) | 2007-12-12 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1179640C (en) * | 2002-11-01 | 2004-12-15 | 中国科学院海洋研究所 | Method for cultivating novel species of Huliotis discus hannai Ino with tangerine color shell |
| CN103109765B (en) * | 2013-02-22 | 2014-11-12 | 厦门大学 | Seed production method by interspecific crossing of green abalone and Pacific abalone |
| CN104335935B (en) * | 2013-12-30 | 2016-04-27 | 中国科学院海洋研究所 | A kind of breeding method improving cultivation Bao Duxia survival rate |
| CN109984062B (en) * | 2018-12-29 | 2021-07-27 | 广西桂螺农业科技有限公司 | Breeding method of escargots |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191068A (en) * | 1997-11-04 | 1998-08-26 | 赵洪恩 | Artificial abalone fry culturing process |
| CN1582650A (en) * | 2004-05-25 | 2005-02-23 | 中国水产科学研究院淡水渔业研究中心 | Hybrid method of Afria crucia carp and mandarin fish |
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2005
- 2005-09-01 CN CNB2005100967701A patent/CN100353922C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191068A (en) * | 1997-11-04 | 1998-08-26 | 赵洪恩 | Artificial abalone fry culturing process |
| CN1582650A (en) * | 2004-05-25 | 2005-02-23 | 中国水产科学研究院淡水渔业研究中心 | Hybrid method of Afria crucia carp and mandarin fish |
Non-Patent Citations (1)
| Title |
|---|
| 几种营养素对亲鱼繁殖性能的影响 张国辉，高红梅，何瑞国.饲料工业，第26卷第4期 2005 * |
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