CN100348088C - Indoor hardening off method for poplar tissue cultivating seedling - Google Patents
Indoor hardening off method for poplar tissue cultivating seedling Download PDFInfo
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- CN100348088C CN100348088C CNB200410053521XA CN200410053521A CN100348088C CN 100348088 C CN100348088 C CN 100348088C CN B200410053521X A CNB200410053521X A CN B200410053521XA CN 200410053521 A CN200410053521 A CN 200410053521A CN 100348088 C CN100348088 C CN 100348088C
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Abstract
The present invention relates to a high speed propagation technology for the tissue culture of plants, particularly to an indoor seedling hardening method aiming at the tissue culture seedlings of poplars. The indoor seedling hardening method for the tissue culture seedlings of poplars is an auxiliary seedling hardening method in which indoor seedling hardening is additionally carried out before the tissue culture seedlings of poplars are transplanted into a greenhouse. In the concrete method, the tissue culture seedlings of poplars which already grow roots are cultured in a sterile environment so that the plant height reaches more than 3 centimeters; indoor culture for 7 days is then carried out under the culture conditions that the time of light irradiation every day is equal to 14 hours, and the intensity of light irradiation ranges from 3000 to 5000 Lux; in the indoor seedling hardening process in 7 days, a cover of a culture flask is gradually opened, and the air humidity is kept at more than 60%. The method of the present invention has the advantages of safety and reliability; after indoor seedling hardening, transplantation into the greenhouse is carried out; consequently, the survival rate of transplantation is ensured to be more than 90%.
Description
Technical field
The present invention relates to a kind of Plant Tissue Breeding quick propagating technology, especially at Yang Yangshu tissue cultivating seedling indoor hardening off method.
Background technology
Plant tissue culture course comprises the explant collection, a series of processes such as culture medium preparation, can, sterilization, deposit, inoculation, cultivation.The willow tissue cultivating seedling is through after the culture of rootage, and here the easy dehydration of direct transplanting booth hardening withers, and survival rate is influenced.For guaranteeing booth hardening willow tissue cultivating seedling survival rate, the invention provides a kind of indoor hardening off method.
Summary of the invention
The object of the invention is to provide a kind of willow tissue cultivating seedling indoor hardening off method, can guarantee transplanting survival rate.
Willow tissue cultivating seedling of the present invention is before transplanting booth, and we have increased this auxiliary hardening off method of indoor hardening.A kind of willow tissue cultivating seedling indoor hardening off method, be that the willow tissue cultivating seedling is before transplanting booth, this the auxiliary hardening off method of indoor hardening that increases, concrete grammar is: under gnotobasis, the willow tissue cultivating seedling plant height of having taken root is cultured to more than 3 centimetres, cultivated again 7 days indoor then, condition of culture is: every day, light application time was 14 hours, and intensity of illumination 3000-5000Lux is in 7 days indoor hardening process, open the cultivation bottle cap gradually, and keep the air humidity more than 60%.
The inventive method is safe and reliable, after indoor hardening, is transplanted to booth again, can guarantee that transplanting survival rate is more than 90%.
Embodiment
A kind of willow tissue cultivating seedling of the present invention indoor hardening off method, system is cultured to the willow tissue cultivating seedling plant height of having taken root more than 3 centimetres, cultivated again 7 days indoor then, condition of culture is: every day, light application time was 14 hours, intensity of illumination 3000-5000Lux, in 7 days indoor hardening process, open the cultivation bottle cap gradually, and keep the air humidity more than 60%; First day, unclamp bottle cap; Second day, open 0.5 centimetre of slit, and in 14 hours light application times, every 2 hours from the slit to the tissue cultivating seedling atomized water spray once; The 3rd day, open 1 centimetre of slit, from the slit spraying once every 2 hours; The 4th day, every 3 hours once from the slit spraying; The 5th day, the slit of opening bottleneck 1/3, every 3 hours once from the slit spraying; The 6th day, every 4 hours once from the slit spraying; The 7th day, bottle cap was all opened, every 4 hours from the slit spraying once; Transplant the booth seedbed behind the 8th day clean medium.In 7 days indoor hardening processes, by effective disinfectant measure, guarantee the cleanliness factor of indoor seeding room surrounding air in 7 days, measure the environment bacterium rate that falls with " plate sedimentation " and be " 0 ".The willow tissue cultivating seedling can reach two purposes by above method before transplanting booth, (1) from aseptic to the adaptation that the collarium border is arranged; (2) saturated environment humidity is to the adaptation of natural environment humidity, reaching the purpose in strong sprout, thereby improves the survival rate of booth hardening.
Claims (2)
1, a kind of willow tissue cultivating seedling indoor hardening off method, be that the willow tissue cultivating seedling is before transplanting booth, this the auxiliary hardening off method of indoor hardening that increases, concrete grammar is: under gnotobasis, the willow tissue cultivating seedling plant height of having taken root is cultured to more than 3 centimetres, cultivated again 7 days indoor then, condition of culture is: every day, light application time was 14 hours, intensity of illumination 3000-5000Lux in 7 days indoor hardening process, opens the cultivation bottle cap gradually, and keep air humidity more than 60%, wherein, the described method of cultivating bottle cap of opening gradually is: first day, unclamp bottle cap; Second day, open 0.5 centimetre of slit, and in 14 hours light application times, every 2 hours from the slit to the tissue cultivating seedling atomized water spray once; The 3rd day, open 1 centimetre of slit, from the slit spraying once every 2 hours; The 4th day, every 3 hours once from the slit spraying; The 5th day, 1/3 slit of opening bottleneck, every 3 hours once from the slit spraying; The 6th day, every 4 hours once from the slit spraying; The 7th day, bottle cap was all opened, every spraying in 4 hours once; Transplant the booth seedbed behind the 8th day clean medium.
2, willow tissue cultivating seedling indoor hardening off method according to claim 1, it is characterized in that described gnotobasis is in 7 days indoor hardening processes, by sterilization, guarantees the cleanliness factor of indoor seeding room surrounding air in 7 days, be " 0 " with " plate sedimentation " mensuration environment bacterium rate that falls.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410053521XA CN100348088C (en) | 2004-08-06 | 2004-08-06 | Indoor hardening off method for poplar tissue cultivating seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410053521XA CN100348088C (en) | 2004-08-06 | 2004-08-06 | Indoor hardening off method for poplar tissue cultivating seedling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1586143A CN1586143A (en) | 2005-03-02 |
| CN100348088C true CN100348088C (en) | 2007-11-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200410053521XA Expired - Fee Related CN100348088C (en) | 2004-08-06 | 2004-08-06 | Indoor hardening off method for poplar tissue cultivating seedling |
Country Status (1)
| Country | Link |
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| CN (1) | CN100348088C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246700B (en) * | 2011-08-18 | 2012-10-24 | 西南林业大学 | Tissue culture method for populus yunnanensis Dode with tender stem as explant |
| CN102550382B (en) * | 2012-03-20 | 2013-06-19 | 东北林业大学 | Rooting transplanting method for tissue culture poplar seedlings |
| CN108575670A (en) * | 2018-02-02 | 2018-09-28 | 青岛市农业科学研究院 | A kind of apple rootstock tissue culture method for transplanting |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1299586A (en) * | 1999-12-16 | 2001-06-20 | 东北林业大学 | Mountain poplar tissue culture and its fast propagating culture medium |
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2004
- 2004-08-06 CN CNB200410053521XA patent/CN100348088C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1299586A (en) * | 1999-12-16 | 2001-06-20 | 东北林业大学 | Mountain poplar tissue culture and its fast propagating culture medium |
Non-Patent Citations (1)
| Title |
|---|
| 河北杨组培苗优化配套移栽技术途径 冯谦 等.东北林业大学学报，第31卷第5期 2003 * |
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| Publication number | Publication date |
|---|---|
| CN1586143A (en) | 2005-03-02 |
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Granted publication date: 20071114 Termination date: 20100806 |