CL2023003646A1 - Métodos de purificación de columnas de vectores aav. - Google Patents
Métodos de purificación de columnas de vectores aav.Info
- Publication number
- CL2023003646A1 CL2023003646A1 CL2023003646A CL2023003646A CL2023003646A1 CL 2023003646 A1 CL2023003646 A1 CL 2023003646A1 CL 2023003646 A CL2023003646 A CL 2023003646A CL 2023003646 A CL2023003646 A CL 2023003646A CL 2023003646 A1 CL2023003646 A1 CL 2023003646A1
- Authority
- CL
- Chile
- Prior art keywords
- purification methods
- aav vectors
- column purification
- full
- particles
- Prior art date
Links
- 238000000746 purification Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000013598 vector Substances 0.000 title 1
- 239000002245 particle Substances 0.000 abstract 4
- 238000002360 preparation method Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163209680P | 2021-06-11 | 2021-06-11 | |
| US202263366094P | 2022-06-09 | 2022-06-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2023003646A1 true CL2023003646A1 (es) | 2024-10-25 |
Family
ID=84426294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2023003646A CL2023003646A1 (es) | 2021-06-11 | 2023-12-05 | Métodos de purificación de columnas de vectores aav. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20240271159A1 (https=) |
| EP (1) | EP4351755A4 (https=) |
| JP (1) | JP2024525142A (https=) |
| KR (1) | KR20240021231A (https=) |
| AU (1) | AU2022289035A1 (https=) |
| BR (1) | BR112023025999A2 (https=) |
| CA (1) | CA3221540A1 (https=) |
| CL (1) | CL2023003646A1 (https=) |
| CO (1) | CO2023018191A2 (https=) |
| IL (1) | IL309070A (https=) |
| MX (1) | MX2023014809A (https=) |
| PE (1) | PE20240359A1 (https=) |
| WO (1) | WO2022261663A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024036251A1 (en) * | 2022-08-12 | 2024-02-15 | Ultragenyx Pharmaceutical Inc. | Novel anion-exchange chromatography methods for separation of empty from full recombinant adeno-associated virus particles |
| EP4670812A1 (en) * | 2024-06-27 | 2025-12-31 | Sartorius Stedim Biotech GmbH | CONTINUOUS POLISHING OF VIRAL CAPSIDES |
| WO2026064763A1 (en) * | 2024-09-23 | 2026-03-26 | Regeneron Pharmaceuticals, Inc. | Partitioning anion exchange chromatography for purification of recombinant adeno-associated virus (raav) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE602004030327D1 (de) * | 2003-05-21 | 2011-01-13 | Genzyme Corp | Verfahren zur herstellung von präparationen rekombinanter aav-virionen, die weitgehend frei von leeren capsiden sind |
| US20120093775A1 (en) * | 2009-03-27 | 2012-04-19 | Proyecto De Biomedicina Cima, S.L. | Methods and compositions for the treatment of cirrhosis and liver fibrosis |
| MY207534A (en) * | 2017-06-30 | 2025-03-03 | Spark Therapeutics Inc | Aav vector column purification methods |
| US20230183659A1 (en) * | 2021-10-29 | 2023-06-15 | Homology Medicines, Inc. | Methods and compositions for the purification of adeno-associated virus |
| EP4602172A1 (en) * | 2022-10-11 | 2025-08-20 | MeiraGTx UK II Limited | Chromatography methods for purification of aav capsids |
-
2022
- 2022-06-10 EP EP22821261.9A patent/EP4351755A4/en active Pending
- 2022-06-10 US US18/568,530 patent/US20240271159A1/en active Pending
- 2022-06-10 MX MX2023014809A patent/MX2023014809A/es unknown
- 2022-06-10 WO PCT/US2022/072859 patent/WO2022261663A1/en not_active Ceased
- 2022-06-10 KR KR1020247000822A patent/KR20240021231A/ko active Pending
- 2022-06-10 PE PE2023003271A patent/PE20240359A1/es unknown
- 2022-06-10 CA CA3221540A patent/CA3221540A1/en active Pending
- 2022-06-10 BR BR112023025999A patent/BR112023025999A2/pt unknown
- 2022-06-10 IL IL309070A patent/IL309070A/en unknown
- 2022-06-10 JP JP2023576326A patent/JP2024525142A/ja active Pending
- 2022-06-10 AU AU2022289035A patent/AU2022289035A1/en active Pending
-
2023
- 2023-12-05 CL CL2023003646A patent/CL2023003646A1/es unknown
- 2023-12-21 CO CONC2023/0018191A patent/CO2023018191A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024525142A (ja) | 2024-07-10 |
| IL309070A (en) | 2024-02-01 |
| US20240271159A1 (en) | 2024-08-15 |
| CO2023018191A2 (es) | 2024-01-25 |
| WO2022261663A1 (en) | 2022-12-15 |
| EP4351755A4 (en) | 2025-04-23 |
| EP4351755A1 (en) | 2024-04-17 |
| AU2022289035A1 (en) | 2024-01-04 |
| BR112023025999A2 (pt) | 2024-02-27 |
| PE20240359A1 (es) | 2024-02-27 |
| KR20240021231A (ko) | 2024-02-16 |
| CA3221540A1 (en) | 2022-12-15 |
| MX2023014809A (es) | 2024-01-29 |
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