CA3221540A1 - Aav vector column purification methods - Google Patents

Aav vector column purification methods Download PDF

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Publication number
CA3221540A1
CA3221540A1 CA3221540A CA3221540A CA3221540A1 CA 3221540 A1 CA3221540 A1 CA 3221540A1 CA 3221540 A CA3221540 A CA 3221540A CA 3221540 A CA3221540 A CA 3221540A CA 3221540 A1 CA3221540 A1 CA 3221540A1
Authority
CA
Canada
Prior art keywords
full
particles
column
raav
chromatography medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3221540A
Other languages
English (en)
French (fr)
Inventor
Ohnmar KHANAL
Vijesh KUMAR
Mi JIN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spark Therapeutics Inc
Original Assignee
Spark Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spark Therapeutics Inc filed Critical Spark Therapeutics Inc
Publication of CA3221540A1 publication Critical patent/CA3221540A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CA3221540A 2021-06-11 2022-06-10 Aav vector column purification methods Pending CA3221540A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202163209680P 2021-06-11 2021-06-11
US63/209,680 2021-06-11
US202263366094P 2022-06-09 2022-06-09
US63/366,094 2022-06-09
PCT/US2022/072859 WO2022261663A1 (en) 2021-06-11 2022-06-10 Aav vector column purification methods

Publications (1)

Publication Number Publication Date
CA3221540A1 true CA3221540A1 (en) 2022-12-15

Family

ID=84426294

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3221540A Pending CA3221540A1 (en) 2021-06-11 2022-06-10 Aav vector column purification methods

Country Status (13)

Country Link
US (1) US20240271159A1 (https=)
EP (1) EP4351755A4 (https=)
JP (1) JP2024525142A (https=)
KR (1) KR20240021231A (https=)
AU (1) AU2022289035A1 (https=)
BR (1) BR112023025999A2 (https=)
CA (1) CA3221540A1 (https=)
CL (1) CL2023003646A1 (https=)
CO (1) CO2023018191A2 (https=)
IL (1) IL309070A (https=)
MX (1) MX2023014809A (https=)
PE (1) PE20240359A1 (https=)
WO (1) WO2022261663A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024036251A1 (en) * 2022-08-12 2024-02-15 Ultragenyx Pharmaceutical Inc. Novel anion-exchange chromatography methods for separation of empty from full recombinant adeno-associated virus particles
EP4670812A1 (en) * 2024-06-27 2025-12-31 Sartorius Stedim Biotech GmbH CONTINUOUS POLISHING OF VIRAL CAPSIDES
WO2026064763A1 (en) * 2024-09-23 2026-03-26 Regeneron Pharmaceuticals, Inc. Partitioning anion exchange chromatography for purification of recombinant adeno-associated virus (raav)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602004030327D1 (de) * 2003-05-21 2011-01-13 Genzyme Corp Verfahren zur herstellung von präparationen rekombinanter aav-virionen, die weitgehend frei von leeren capsiden sind
US20120093775A1 (en) * 2009-03-27 2012-04-19 Proyecto De Biomedicina Cima, S.L. Methods and compositions for the treatment of cirrhosis and liver fibrosis
MY207534A (en) * 2017-06-30 2025-03-03 Spark Therapeutics Inc Aav vector column purification methods
US20230183659A1 (en) * 2021-10-29 2023-06-15 Homology Medicines, Inc. Methods and compositions for the purification of adeno-associated virus
EP4602172A1 (en) * 2022-10-11 2025-08-20 MeiraGTx UK II Limited Chromatography methods for purification of aav capsids

Also Published As

Publication number Publication date
JP2024525142A (ja) 2024-07-10
IL309070A (en) 2024-02-01
US20240271159A1 (en) 2024-08-15
CO2023018191A2 (es) 2024-01-25
WO2022261663A1 (en) 2022-12-15
EP4351755A4 (en) 2025-04-23
EP4351755A1 (en) 2024-04-17
AU2022289035A1 (en) 2024-01-04
BR112023025999A2 (pt) 2024-02-27
PE20240359A1 (es) 2024-02-27
KR20240021231A (ko) 2024-02-16
CL2023003646A1 (es) 2024-10-25
MX2023014809A (es) 2024-01-29

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