CL2018000872A1 - Métodos de preservar la actividad biológica de los ácidos ribonucleicos. - Google Patents

Métodos de preservar la actividad biológica de los ácidos ribonucleicos.

Info

Publication number
CL2018000872A1
CL2018000872A1 CL2018000872A CL2018000872A CL2018000872A1 CL 2018000872 A1 CL2018000872 A1 CL 2018000872A1 CL 2018000872 A CL2018000872 A CL 2018000872A CL 2018000872 A CL2018000872 A CL 2018000872A CL 2018000872 A1 CL2018000872 A1 CL 2018000872A1
Authority
CL
Chile
Prior art keywords
preserving
biological activity
methods
ribonucleic acids
lysate
Prior art date
Application number
CL2018000872A
Other languages
English (en)
Inventor
Pascale Feldmann
Jeffrey David Fowler
Wendy Maddelein
Isabelle Maillet
Nina Cromheecke
Original Assignee
Syngenta Participations Ag
Devgen Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, Devgen Nv filed Critical Syngenta Participations Ag
Publication of CL2018000872A1 publication Critical patent/CL2018000872A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

<p>La presente invención proporciona un método de conservar sustancialmente o preservar de otra forma la actividad biológica de un ARNbc, presente en un lisado celular, para silenciar de forma postranscripcional la expresión de un gen en un organismo diana, que comprende la etapa de añadir al lisado un compuesto que tiene la función de agente reticulante - proteico o de tipo amina -. La invención también comprende composiciones que comprenden el lisado que comprende ARNbc y agentes reticulantes proteicos, así como el uso de dichos agentes en el método.</p>
CL2018000872A 2015-10-05 2018-04-04 Métodos de preservar la actividad biológica de los ácidos ribonucleicos. CL2018000872A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562237055P 2015-10-05 2015-10-05

Publications (1)

Publication Number Publication Date
CL2018000872A1 true CL2018000872A1 (es) 2018-07-06

Family

ID=57133132

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2018000872A CL2018000872A1 (es) 2015-10-05 2018-04-04 Métodos de preservar la actividad biológica de los ácidos ribonucleicos.

Country Status (15)

Country Link
US (1) US20180289015A1 (es)
EP (1) EP3358956A1 (es)
JP (1) JP2018529386A (es)
KR (1) KR20180056750A (es)
CN (1) CN108135182A (es)
AR (1) AR106259A1 (es)
AU (2) AU2016335158A1 (es)
BR (1) BR112018006358A2 (es)
CA (1) CA2998195A1 (es)
CL (1) CL2018000872A1 (es)
IL (1) IL257959A (es)
PH (1) PH12018500744A1 (es)
RU (1) RU2018116201A (es)
WO (1) WO2017060122A1 (es)
ZA (1) ZA201802836B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3522719A1 (en) * 2016-10-05 2019-08-14 Syngenta Participations AG Methods of preserving the biological activity of ribonucleic acids

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2500224C (en) * 2002-09-25 2015-04-28 University Of Massachusetts In vivo gene silencing by chemically modified and stable sirna
CA2562022C (en) * 2004-04-09 2016-01-26 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
WO2006093083A1 (ja) * 2005-03-03 2006-09-08 Wako Pure Chemical Industries, Ltd. 架橋剤、架橋法、遺伝子発現調節法及び遺伝子機能調査法
JP4645234B2 (ja) * 2005-03-03 2011-03-09 和光純薬工業株式会社 架橋剤、それを用いた架橋方法、遺伝子発現調節方法および遺伝子機能調査方法
US20100129460A1 (en) * 2005-10-14 2010-05-27 Nastech Pharmaceutical Company Inc. Compounds and methods for peptide ribonucleic acid condensate particles for rna therapeutics
EP2175020A1 (en) * 2008-10-13 2010-04-14 Roche Diagnostics GmbH Reduction of RNase activity in complex fluidic samples
US20100257634A1 (en) * 2009-04-03 2010-10-07 Venganza Inc. Bioassay for gene silencing constructs
CA2768598A1 (en) * 2009-07-22 2011-01-27 Cenix Bioscience Gmbh Delivery system and conjugates for compound delivery via naturally occurring intracellular transport routes
GB2481637B (en) * 2010-07-01 2013-11-20 Custompac Ltd A method of manufacturing artificial snow
JP6240599B2 (ja) * 2011-07-19 2017-11-29 セルモザイク， インコーポレイテッド 新規の架橋試薬、高分子、治療用コンジュゲートおよびその合成法
EP2970889A4 (en) * 2013-03-14 2016-10-19 Univ Georgia State Res Found PREVENTING OR DELAYING RESPONSE TO COLD-DAMAGE IN PLANTS
EP3434777B1 (en) * 2013-03-15 2020-09-23 Monsanto Technology LLC Compositions and methods for the production and delivery of rna

Also Published As

Publication number Publication date
RU2018116201A3 (es) 2020-02-28
AU2021201421A1 (en) 2021-03-25
CA2998195A1 (en) 2017-04-13
AR106259A1 (es) 2017-12-27
BR112018006358A2 (pt) 2018-10-09
WO2017060122A1 (en) 2017-04-13
PH12018500744A1 (en) 2018-10-15
AU2016335158A1 (en) 2018-04-12
CN108135182A (zh) 2018-06-08
JP2018529386A (ja) 2018-10-11
IL257959A (en) 2018-05-31
ZA201802836B (en) 2019-02-27
RU2018116201A (ru) 2019-11-07
EP3358956A1 (en) 2018-08-15
KR20180056750A (ko) 2018-05-29
US20180289015A1 (en) 2018-10-11

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