CL2015003402A1 - Células madres adultas adiposas, humanas, pluripotentes: aislamiento, caracterización e implicaciones clinicas - Google Patents

Células madres adultas adiposas, humanas, pluripotentes: aislamiento, caracterización e implicaciones clinicas

Info

Publication number
CL2015003402A1
CL2015003402A1 CL2015003402A CL2015003402A CL2015003402A1 CL 2015003402 A1 CL2015003402 A1 CL 2015003402A1 CL 2015003402 A CL2015003402 A CL 2015003402A CL 2015003402 A CL2015003402 A CL 2015003402A CL 2015003402 A1 CL2015003402 A1 CL 2015003402A1
Authority
CL
Chile
Prior art keywords
characterization
fat
isolation
human
stem cells
Prior art date
Application number
CL2015003402A
Other languages
English (en)
Inventor
Gregorio Chazenbalk
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of CL2015003402A1 publication Critical patent/CL2015003402A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1352—Mesenchymal stem cells
    • C12N2502/1382—Adipose-derived stem cells [ADSC], adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1384—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from adipose-derived stem cells [ADSC], from adipose stromal stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Diabetes (AREA)
  • Emergency Medicine (AREA)
  • Cardiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Obesity (AREA)
  • Dermatology (AREA)
  • Endocrinology (AREA)

Abstract

MÉTODO PARA AISLAR CÉLULAS MADRES PLURIPOTENTES (PSCs) DE TEJIDO ADIPOSO; COMPOSICIÓN QUE COMPRENDE PSCs; Y USO PARA ALIVIAR DAÑO DEL TEJIDO EN UN SUJETO.
CL2015003402A 2013-05-22 2015-11-19 Células madres adultas adiposas, humanas, pluripotentes: aislamiento, caracterización e implicaciones clinicas CL2015003402A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361826417P 2013-05-22 2013-05-22

Publications (1)

Publication Number Publication Date
CL2015003402A1 true CL2015003402A1 (es) 2016-07-22

Family

ID=51934148

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2015003402A CL2015003402A1 (es) 2013-05-22 2015-11-19 Células madres adultas adiposas, humanas, pluripotentes: aislamiento, caracterización e implicaciones clinicas

Country Status (5)

Country Link
US (4) US10131880B2 (es)
EP (2) EP3613846A1 (es)
JP (1) JP2016519939A (es)
CL (1) CL2015003402A1 (es)
WO (1) WO2014190150A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200101991A (ko) * 2016-01-15 2020-08-28 국립대학법인 도야마 다이가쿠 허혈성 뇌경색에의 다능성 줄기 세포의 동원
US20190391147A1 (en) * 2016-04-07 2019-12-26 Rowan University Methods and kits for detecting myo/nog cells in human adipose tissue and methods of use thereof
EP3791888A4 (en) * 2018-05-09 2022-03-23 Life Science Institute, Inc. THERAPEUTIC AGENT FOR SPINAL CORD INJURY
CN116056716A (zh) * 2020-05-15 2023-05-02 首尔大学校产学协力团 利用从脂肪组织分离出来的基质血管组分的树突状细胞的激活功能的免疫反应增强用组合物
JP6864302B1 (ja) * 2020-07-03 2021-04-28 富士フイルム株式会社 滑膜由来間葉系幹細胞の製造方法および関節治療用細胞製剤の製造方法
CN117187174B (zh) * 2023-11-08 2024-02-06 广州正源生物技术有限公司 一种Muse细胞培养基以及一种脂肪Muse细胞的提取方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030082152A1 (en) 1999-03-10 2003-05-01 Hedrick Marc H. Adipose-derived stem cells and lattices
NZ518601A (en) 1999-09-24 2004-10-29 Morphogen Pharmaceuticals Inc Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
ATE384124T1 (de) * 2000-02-26 2008-02-15 Artecel Inc Pluripotente stammzellen erzeugt aus stromalzellen aus dem fettgewebe und deren verwendungen
DE10326750B4 (de) * 2003-06-13 2006-07-27 Gerlach, Jörg, Dr.med. Verfahren zur Herstellung einer Zellpräparation und derart hergestellte Zellpräparationen
US8119398B2 (en) 2004-12-30 2012-02-21 Primegen Biotech Llc Adipose-derived stem cells for tissue regeneration and wound healing
KR100679642B1 (ko) * 2005-11-16 2007-02-06 주식회사 알앤엘바이오 인간 지방조직 유래 다분화능 줄기세포 및 이를 함유하는세포치료제
US20090304644A1 (en) * 2006-05-30 2009-12-10 Cytori Therapeutics, Inc. Systems and methods for manipulation of regenerative cells separated and concentrated from adipose tissue
WO2008103807A2 (en) 2007-02-21 2008-08-28 Cedars-Sinai Medical Center Methods of producing preadipocytes and increasing the proliferation of adult adipose stem/progenitor cells
WO2010069008A1 (en) 2008-12-19 2010-06-24 Griffith University A germline competent cell derived from adult tissue
EP2446024A1 (en) * 2009-06-26 2012-05-02 Life&Light Limited Mesenchymal stem cells grown under hypoxic conditions: compositions, methods and uses therefor
JP2014132830A (ja) * 2011-03-30 2014-07-24 Clio Inc 生体の臍帯又は脂肪組織から単離できる多能性幹細胞
US9399758B2 (en) * 2009-07-15 2016-07-26 Mari Dezawa SSEA3(+) pluripotent stem cell that can be isolated from body tissue
US9550975B2 (en) * 2009-07-15 2017-01-24 Mari Dezawa SSEA-3 pluripotent stem cell isolated from body tissue
US8501397B2 (en) * 2009-07-24 2013-08-06 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Enrichment of stem cells from adult tissues
EP2482761A4 (en) 2009-09-30 2014-03-19 Brigham & Womens Hospital TISSUE TRANSPLANT COMPOSITIONS AND METHOD FOR THEIR USE
CN102725399B (zh) * 2009-12-18 2015-03-11 上海赛傲生物技术有限公司 制备多能干细胞的材料和方法
US20120028933A1 (en) * 2010-07-28 2012-02-02 Baust John M Cell Culture Media Supplement and Method of Molecular Stress Control

Also Published As

Publication number Publication date
US20160108364A1 (en) 2016-04-21
US20190136193A1 (en) 2019-05-09
US11066647B2 (en) 2021-07-20
EP2999780A4 (en) 2016-11-16
WO2014190150A1 (en) 2014-11-27
US11913027B2 (en) 2024-02-27
EP2999780B1 (en) 2019-10-09
EP3613846A1 (en) 2020-02-26
US20210403872A1 (en) 2021-12-30
JP2016519939A (ja) 2016-07-11
US10131880B2 (en) 2018-11-20
US20240076623A1 (en) 2024-03-07
EP2999780A1 (en) 2016-03-30

Similar Documents

Publication Publication Date Title
CL2015003402A1 (es) Células madres adultas adiposas, humanas, pluripotentes: aislamiento, caracterización e implicaciones clinicas
MX2019014009A (es) Metodos mejorados para la elaboracion de terapias celulares adoptivas.
BR112018068126A2 (pt) sistemas e métodos para a produção e liberação de pasta fluida gelada medicinal
TW201612312A (en) Meristematic plant cells and method of isolating them
UY34577A (es) Método para estabilizar fibra de lignina y su posterior conversión en fibra de carbono
ES2721180T3 (es) Productos terapéuticos usando células adiposas y secreciones celulares
TWD170564S (zh) 杯子
DK3325025T3 (da) Sammensætning og produkter omfattende aldrende celler til anvendelse i vævsregeneration
MX342200B (es) Método de tratamiento de células madre mesenquimales y su uso en el tratamiento de enfermedades asociadas a estrés oxidativo.
EP2864477A4 (en) ISOLATION OF STEM CELLS FROM ADIPOSE TISSUE BY ULTRASONIC CAVITATION, AND METHODS OF USE
EP3021791A4 (en) Tissue interface augmentation device for ligament/tendon reconstruction
BR112016030758B8 (pt) Uso de células estromais multipotentes derivadas de tecido adiposo mesenquimal
BR112016029558A2 (pt) terapia de células-tronco em patologias endometriais
WO2014124307A3 (en) Targeted theranostics
BR112014019328A8 (pt) Perfil de fenótipo de células progenitoras retinais humanas
WO2014138426A3 (en) Methods and compositions for reducing body fat and adipocytes
IL274995B (en) Stem cells extracted from brown fat and their use
UY35438A (es) Procedimiento para preparar inhibidores de la glucosilceramida sintasa.
CL2015001448A1 (es) Método para sincronización del tiempo de inseminación en cerdas jovenes
EP3189844A4 (en) Pluripotent stem cell for treating diabetic skin ulcer
BR112018008931A2 (pt) ?compostos para tratamento de distúrbios hipoproliferativos?
BR112014021326A2 (pt) célula para a expressão de protéina, método para reduzir a atividade de amidação de peptídeo em uma determinada célula, uso de uma célula
HK1245327A1 (zh) 使用表達lgr4、lgr5和lgr6的上皮幹細胞在組織應用中開發和使用最小極性化功能細胞微聚集體單元的方法
SG11201903231WA (en) Microbioreactor module
IN2015DN00172A (es)