CH638053A5 - Process for the determination of vitamin B12 in a liquid - Google Patents
Process for the determination of vitamin B12 in a liquid Download PDFInfo
- Publication number
- CH638053A5 CH638053A5 CH1274878A CH1274878A CH638053A5 CH 638053 A5 CH638053 A5 CH 638053A5 CH 1274878 A CH1274878 A CH 1274878A CH 1274878 A CH1274878 A CH 1274878A CH 638053 A5 CH638053 A5 CH 638053A5
- Authority
- CH
- Switzerland
- Prior art keywords
- vitamin
- mixture
- serum
- binding proteins
- labeled
- Prior art date
Links
- 239000011715 vitamin B12 Substances 0.000 title claims description 77
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 title claims description 74
- 229930003779 Vitamin B12 Natural products 0.000 title claims description 73
- 235000019163 vitamin B12 Nutrition 0.000 title claims description 73
- 238000000034 method Methods 0.000 title claims description 47
- 239000007788 liquid Substances 0.000 title claims description 16
- 210000002966 serum Anatomy 0.000 claims description 73
- 239000000203 mixture Substances 0.000 claims description 27
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 26
- 102000014914 Carrier Proteins Human genes 0.000 claims description 21
- 108091008324 binding proteins Proteins 0.000 claims description 21
- 239000002245 particle Substances 0.000 claims description 17
- 229930003231 vitamin Natural products 0.000 claims description 16
- 235000013343 vitamin Nutrition 0.000 claims description 16
- 239000011782 vitamin Substances 0.000 claims description 16
- 229940088594 vitamin Drugs 0.000 claims description 16
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 15
- 239000000872 buffer Substances 0.000 claims description 13
- 102000011409 Transcobalamins Human genes 0.000 claims description 7
- 108010023603 Transcobalamins Proteins 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 239000011236 particulate material Substances 0.000 claims description 7
- 230000027455 binding Effects 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 238000010411 cooking Methods 0.000 claims description 5
- 239000011343 solid material Substances 0.000 claims description 4
- 108010088751 Albumins Proteins 0.000 claims description 3
- 102000009027 Albumins Human genes 0.000 claims description 3
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 claims description 3
- HFWIMJHBCIGYFH-UHFFFAOYSA-N cyanoform Chemical compound N#CC(C#N)C#N HFWIMJHBCIGYFH-UHFFFAOYSA-N 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- -1 cobalt vitamin Chemical class 0.000 claims description 2
- 238000001179 sorption measurement Methods 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims 2
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- 238000010790 dilution Methods 0.000 description 10
- 239000012895 dilution Substances 0.000 description 10
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 230000009137 competitive binding Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 238000009835 boiling Methods 0.000 description 3
- 238000010494 dissociation reaction Methods 0.000 description 3
- 230000005593 dissociations Effects 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 2
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 241000195619 Euglena gracilis Species 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 2
- 239000001099 ammonium carbonate Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 2
- 231100000673 dose–response relationship Toxicity 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000013048 microbiological method Methods 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102400001066 Growth hormone-binding protein Human genes 0.000 description 1
- 101800000194 Growth hormone-binding protein Proteins 0.000 description 1
- 101000798415 Homo sapiens BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 Proteins 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910001567 cementite Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- KXZJHVJKXJLBKO-UHFFFAOYSA-N chembl1408157 Chemical compound N=1C2=CC=CC=C2C(C(=O)O)=CC=1C1=CC=C(O)C=C1 KXZJHVJKXJLBKO-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229940029329 intrinsic factor Drugs 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 102000028728 vitamin binding proteins Human genes 0.000 description 1
- 108091009357 vitamin binding proteins Proteins 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB5206977 | 1977-12-14 | ||
| GB7830301 | 1978-07-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH638053A5 true CH638053A5 (en) | 1983-08-31 |
Family
ID=26266985
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH1274878A CH638053A5 (en) | 1977-12-14 | 1978-12-14 | Process for the determination of vitamin B12 in a liquid |
Country Status (7)
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989012826A1 (en) * | 1988-06-15 | 1989-12-28 | Beckman Instruments, Inc. | Vitamin b12 assay |
-
1978
- 1978-12-11 AU AU42396/78A patent/AU519485B2/en not_active Expired
- 1978-12-12 FR FR7834937A patent/FR2412074A1/fr active Granted
- 1978-12-12 DE DE19782853500 patent/DE2853500A1/de active Granted
- 1978-12-13 NL NL7812106A patent/NL7812106A/xx not_active Application Discontinuation
- 1978-12-13 CA CA317,888A patent/CA1104911A/en not_active Expired
- 1978-12-14 CH CH1274878A patent/CH638053A5/de not_active IP Right Cessation
- 1978-12-14 JP JP15376978A patent/JPS54116293A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE2853500C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-04-14 |
| AU4239678A (en) | 1979-06-21 |
| CA1104911A (en) | 1981-07-14 |
| JPS6342231B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-08-22 |
| NL7812106A (nl) | 1979-06-18 |
| FR2412074B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1984-05-25 |
| FR2412074A1 (fr) | 1979-07-13 |
| JPS54116293A (en) | 1979-09-10 |
| AU519485B2 (en) | 1981-12-03 |
| DE2853500A1 (de) | 1979-06-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUE | Assignment |
Owner name: TECHNICON INSTRUMENTS CORPORATION TRANSFER- TECHNI |
|
| PL | Patent ceased | ||
| PL | Patent ceased |