CA3262557A1 - Modified transposases for improved insertion sequence bias and increased dna input tolerance - Google Patents
Modified transposases for improved insertion sequence bias and increased dna input toleranceInfo
- Publication number
- CA3262557A1 CA3262557A1 CA3262557A CA3262557A CA3262557A1 CA 3262557 A1 CA3262557 A1 CA 3262557A1 CA 3262557 A CA3262557 A CA 3262557A CA 3262557 A CA3262557 A CA 3262557A CA 3262557 A1 CA3262557 A1 CA 3262557A1
- Authority
- CA
- Canada
- Prior art keywords
- insertion sequence
- increased dna
- dna input
- improved insertion
- sequence bias
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461979871P | 2014-04-15 | 2014-04-15 | |
| US61/979,871 | 2014-04-15 | ||
| US201462062006P | 2014-10-09 | 2014-10-09 | |
| US62/062,006 | 2014-10-09 | ||
| US201462080882P | 2014-11-17 | 2014-11-17 | |
| US62/080,882 | 2014-11-17 | ||
| CA2946046A CA2946046A1 (en) | 2014-04-15 | 2015-04-15 | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2946046A Division CA2946046A1 (en) | 2014-04-15 | 2015-04-15 | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3262557A1 true CA3262557A1 (en) | 2026-03-02 |
Family
ID=53008897
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3262557A Pending CA3262557A1 (en) | 2014-04-15 | 2015-04-15 | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
| CA2946046A Pending CA2946046A1 (en) | 2014-04-15 | 2015-04-15 | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2946046A Pending CA2946046A1 (en) | 2014-04-15 | 2015-04-15 | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US9790476B2 (enExample) |
| EP (2) | EP3132029B1 (enExample) |
| JP (1) | JP6490710B2 (enExample) |
| CN (3) | CN113005108B (enExample) |
| AU (3) | AU2015247779B2 (enExample) |
| CA (2) | CA3262557A1 (enExample) |
| SG (1) | SG11201608585RA (enExample) |
| TW (1) | TWI682997B (enExample) |
| WO (1) | WO2015160895A2 (enExample) |
Families Citing this family (139)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| WO2015160895A2 (en) | 2014-04-15 | 2015-10-22 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
| CN106715693A (zh) | 2014-06-13 | 2017-05-24 | 伊卢米纳剑桥有限公司 | 用于制备序列文库的方法和组合物 |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| CN112430641A (zh) | 2014-06-30 | 2021-03-02 | 亿明达股份有限公司 | 使用单侧转座的方法和组合物 |
| BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| US10815478B2 (en) * | 2014-11-05 | 2020-10-27 | Illumina, Inc. | Method of sequential tagmentation with transposase compositions for reduction of insertion bias |
| US10900065B2 (en) | 2014-11-14 | 2021-01-26 | University Of Washington | Methods and kits for labeling cellular molecules |
| JP6769969B2 (ja) | 2015-01-12 | 2020-10-14 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| US10988805B2 (en) | 2015-02-20 | 2021-04-27 | The Regents Of The University Of California | Methods related to DNA sequencing |
| MX2017010857A (es) | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| WO2016196358A1 (en) * | 2015-05-29 | 2016-12-08 | Epicentre Technologies Corporation | Methods of analyzing nucleic acids |
| JP6954899B2 (ja) | 2015-12-04 | 2021-10-27 | １０エックス ゲノミクス，インコーポレイテッド | 核酸の解析のための方法及び組成物 |
| WO2017156336A1 (en) * | 2016-03-10 | 2017-09-14 | The Board Of Trustees Of The Leland Stanford Junior University | Transposase-mediated imaging of the accessible genome |
| US11932847B2 (en) * | 2016-04-19 | 2024-03-19 | Kapa Biosystems, Inc. | Transposase competitor control system |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| CN106222164B (zh) * | 2016-07-28 | 2020-04-21 | 元码基因科技（北京）股份有限公司 | 利用转座子酶进行体外核酸单向扩增的方法、组合物和试剂盒 |
| US10676736B2 (en) | 2016-08-05 | 2020-06-09 | Bio-Rad Laboratories, Inc. | Second strand direct |
| ES2922281T3 (es) * | 2016-12-07 | 2022-09-12 | Mgi Tech Co Ltd | Método para construir una biblioteca de secuenciación de una célula individual y uso del mismo |
| CN110139932B (zh) | 2016-12-19 | 2024-05-17 | 生物辐射实验室股份有限公司 | 液滴加标的相邻性保留的标签化dna |
| CN106754811B (zh) * | 2016-12-21 | 2019-05-14 | 南京诺唯赞生物科技有限公司 | 一种突变型Tn5转座酶及其制备方法和应用 |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN106801044B (zh) * | 2017-01-09 | 2021-03-23 | 北京全式金生物技术有限公司 | 一种环状转座子复合物及其应用 |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| KR102866805B1 (ko) | 2017-02-21 | 2025-09-30 | 일루미나, 인코포레이티드 | 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화 |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| EP3676396B1 (en) * | 2017-08-30 | 2022-04-06 | Kapa Biosystems, Inc. | Transposase compositions, methods of making and methods of screening |
| DK4060041T5 (en) | 2017-09-25 | 2024-07-22 | Fred Hutchinson Cancer Center | High efficiency targeted in situ genome-wide profiling |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| WO2019089959A1 (en) | 2017-11-02 | 2019-05-09 | Bio-Rad Laboratories, Inc. | Transposase-based genomic analysis |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| US12377396B2 (en) | 2018-02-05 | 2025-08-05 | The Board Of Trustees Of The Leland Stanford Junior University | Systems and methods for multiplexed measurements in single and ensemble cells |
| CN108285494B (zh) * | 2018-02-11 | 2021-09-07 | 北京大学 | 一种融合蛋白、试剂盒以及CHIP-seq检测方法 |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| WO2019184044A1 (zh) * | 2018-03-27 | 2019-10-03 | 上海欣百诺生物科技有限公司 | 一种转座酶-抗体结合蛋白的融合蛋白及其制备与应用 |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| CN108913712B (zh) * | 2018-07-17 | 2021-04-02 | 厦门生命互联科技有限公司 | 一种重组Tn5转座酶的表达纯化方法 |
| SG11202101164TA (en) | 2018-08-03 | 2021-03-30 | 10X Genomics Inc | Methods and systems to minimize barcode exchange |
| EP4249651B1 (en) | 2018-08-20 | 2025-01-29 | Bio-Rad Laboratories, Inc. | Nucleotide sequence generation by barcode bead-colocalization in partitions |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| CN112867801B (zh) | 2018-11-30 | 2024-07-12 | Illumina公司 | 使用单一测定分析多种分析物 |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| CN112867800B (zh) | 2018-12-17 | 2024-07-12 | Illumina公司 | 用于制备测序文库的方法和手段 |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| IL281664B2 (en) | 2019-01-11 | 2024-07-01 | Illumina Cambridge Ltd | Complex surface-bound transposome complexes |
| EP3924505B1 (en) | 2019-02-12 | 2025-12-17 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| BR112021006173A2 (pt) | 2019-04-29 | 2021-11-16 | Illumina Inc | Métodos para a identificação e análise de micro-organismos viáveis e/ou proliferativos e para determinação da eficácia de um agente antimicrobiano na modulação do crescimento e da proliferação de micro-organismos em uma amostra |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| AU2020312787A1 (en) | 2019-07-12 | 2021-06-17 | Illumina Cambridge Limited | Nucleic acid library preparation using electrophoresis |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| CN110938610B (zh) * | 2019-12-16 | 2020-12-01 | 广东菲鹏生物有限公司 | 一种转座酶突变体、融合蛋白、其制备方法和应用 |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| ES2982420T3 (es) | 2019-12-23 | 2024-10-16 | 10X Genomics Inc | Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| ES2994976T3 (en) | 2020-06-25 | 2025-02-05 | 10X Genomics Inc | Spatial analysis of dna methylation |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| CN116171330A (zh) | 2020-08-06 | 2023-05-26 | Illumina公司 | 使用小珠连接的转座体制备rna和dna测序文库 |
| EP4200416A1 (en) | 2020-08-18 | 2023-06-28 | Illumina, Inc. | Sequence-specific targeted transposition and selection and sorting of nucleic acids |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| CN112164419A (zh) * | 2020-09-04 | 2021-01-01 | 云舟生物科技（广州）有限公司 | 在orf中插入蛋白标签的方法以及计算机存储介质 |
| WO2022056309A1 (en) * | 2020-09-11 | 2022-03-17 | Fred Hutchinson Cancer Research Center | Improved high efficiency targeted in situ genome-wide profiling |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| KR20230091116A (ko) | 2020-10-21 | 2023-06-22 | 일루미나, 인코포레이티드 | 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법 |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| AU2022212231A1 (en) | 2021-01-29 | 2023-08-03 | 10X Genomics, Inc. | Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample |
| EP4288534A1 (en) * | 2021-02-05 | 2023-12-13 | Ospedale San Raffaele S.r.l. | Engineered transposase and uses thereof |
| CN112795563A (zh) * | 2021-03-23 | 2021-05-14 | 上海欣百诺生物科技有限公司 | 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法 |
| WO2022212269A1 (en) | 2021-03-29 | 2022-10-06 | Illumina, Inc. | Improved methods of library preparation |
| MX2023010495A (es) | 2021-03-30 | 2023-09-18 | Illumina Inc | Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos. |
| CA3211172A1 (en) | 2021-03-31 | 2022-10-06 | Illumina, Inc. | Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4334442A1 (en) * | 2021-05-03 | 2024-03-13 | European Molecular Biology Laboratory | Methods for isolating and/or obtaining captured polynucleotide fragments |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| AU2022272723A1 (en) | 2021-05-14 | 2023-11-30 | Becton, Dickinson And Company | Methods for making libraries for nucleic acid sequencing |
| EP4341392A4 (en) | 2021-05-14 | 2025-02-19 | Becton Dickinson and Company | Multiplexed unbiased nucleic acid amplification method |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| CN114807084B (zh) * | 2022-04-26 | 2023-05-16 | 翌圣生物科技(上海)股份有限公司 | 突变型Tn5转座酶及试剂盒 |
| WO2023225519A1 (en) | 2022-05-17 | 2023-11-23 | 10X Genomics, Inc. | Modified transposons, compositions and uses thereof |
| CN115927244B (zh) * | 2022-05-26 | 2026-01-02 | 上海墨卓生物科技有限公司 | 一种基于微流控技术的单细胞ChIP-seq文库的构建方法 |
| CN115948363B (zh) * | 2022-08-26 | 2024-02-27 | 武汉影子基因科技有限公司 | Tn5转座酶突变体及其制备方法和应用 |
| CN115785283B (zh) * | 2022-11-02 | 2024-05-31 | 武汉影子基因科技有限公司 | PAG-Tn5突变体及其应用 |
| EP4482979B1 (en) | 2022-11-09 | 2025-10-01 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
| WO2024137934A1 (en) * | 2022-12-21 | 2024-06-27 | The Trustees Of Columbia University In The City Of New York | Use of pa-tn5 to generate dna libraries |
| US20250207193A1 (en) | 2023-12-22 | 2025-06-26 | Illumina, Inc. | Reusable flow cells and methods of using them for nucleic acid sequencing |
| WO2025244841A1 (en) | 2024-05-23 | 2025-11-27 | Illumina, Inc. | Methods and systems for quantifying targeted enrichment efficiency |
| WO2026006746A2 (en) | 2024-06-28 | 2026-01-02 | Illumina, Inc. | Nucleic acid preparation and analysis techniques |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| US5925545A (en) | 1996-09-09 | 1999-07-20 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US6159736A (en) * | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
| CA2380850A1 (en) * | 1999-08-02 | 2001-02-08 | Wisconsin Alumni Research Foundation | Mutant tn5 transposase enzymes and method for their use |
| US7262056B2 (en) * | 2001-11-08 | 2007-08-28 | Mirus Bio Corporation | Enhancing intermolecular integration of nucleic acids using integrator complexes |
| WO2004093645A2 (en) * | 2003-04-17 | 2004-11-04 | Wisconsin Alumni Research Foundation | Tn5 transposase mutants and the use thereof |
| US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
| KR100890187B1 (ko) | 2007-09-06 | 2009-03-25 | 고려대학교 산학협력단 | Ｔｓｆ를 융합파트너로 이용한 재조합 단백질의 제조방법 |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| CA2750054C (en) | 2008-10-24 | 2018-05-29 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US9005935B2 (en) | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| WO2013085918A1 (en) | 2011-12-05 | 2013-06-13 | The Regents Of The University Of California | Methods and compostions for generating polynucleic acid fragments |
| WO2015160895A2 (en) | 2014-04-15 | 2015-10-22 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
-
2015
- 2015-04-15 WO PCT/US2015/025889 patent/WO2015160895A2/en not_active Ceased
- 2015-04-15 EP EP15719091.9A patent/EP3132029B1/en active Active
- 2015-04-15 EP EP20208323.4A patent/EP3845640A3/en active Pending
- 2015-04-15 CN CN202110295229.2A patent/CN113005108B/zh active Active
- 2015-04-15 CN CN201580027103.XA patent/CN106507677B/zh active Active
- 2015-04-15 TW TW104112180A patent/TWI682997B/zh active
- 2015-04-15 CA CA3262557A patent/CA3262557A1/en active Pending
- 2015-04-15 AU AU2015247779A patent/AU2015247779B2/en active Active
- 2015-04-15 CN CN202510139371.6A patent/CN120098956A/zh active Pending
- 2015-04-15 CA CA2946046A patent/CA2946046A1/en active Pending
- 2015-04-15 US US14/686,961 patent/US9790476B2/en active Active
- 2015-04-15 SG SG11201608585RA patent/SG11201608585RA/en unknown
- 2015-04-15 JP JP2016562588A patent/JP6490710B2/ja active Active
-
2017
- 2017-10-12 US US15/782,757 patent/US10035992B2/en active Active
-
2018
- 2018-06-28 US US16/021,417 patent/US10385323B2/en active Active
-
2019
- 2019-04-29 US US16/397,542 patent/US10544403B2/en active Active
-
2021
- 2021-09-22 AU AU2021236488A patent/AU2021236488B2/en active Active
-
2024
- 2024-07-24 AU AU2024205075A patent/AU2024205075A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US10544403B2 (en) | 2020-01-28 |
| JP2017513479A (ja) | 2017-06-01 |
| CA2946046A1 (en) | 2015-10-22 |
| CN106507677A (zh) | 2017-03-15 |
| US20180171311A1 (en) | 2018-06-21 |
| EP3132029A2 (en) | 2017-02-22 |
| JP6490710B2 (ja) | 2019-03-27 |
| WO2015160895A3 (en) | 2016-01-14 |
| AU2015247779B2 (en) | 2021-06-24 |
| CN120098956A (zh) | 2025-06-06 |
| EP3845640A3 (en) | 2021-09-01 |
| AU2021236488A1 (en) | 2021-10-21 |
| SG11201608585RA (en) | 2016-11-29 |
| CN113005108B (zh) | 2025-02-11 |
| CN106507677B (zh) | 2021-03-12 |
| EP3845640A2 (en) | 2021-07-07 |
| US9790476B2 (en) | 2017-10-17 |
| TWI682997B (zh) | 2020-01-21 |
| US10035992B2 (en) | 2018-07-31 |
| EP3132029B1 (en) | 2026-01-28 |
| CN113005108A (zh) | 2021-06-22 |
| AU2015247779A1 (en) | 2016-11-03 |
| WO2015160895A2 (en) | 2015-10-22 |
| US20150291942A1 (en) | 2015-10-15 |
| TW201621048A (zh) | 2016-06-16 |
| AU2021236488B2 (en) | 2024-06-20 |
| US10385323B2 (en) | 2019-08-20 |
| AU2024205075A1 (en) | 2024-10-03 |
| US20190359955A1 (en) | 2019-11-28 |
| US20180298356A1 (en) | 2018-10-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA3262557A1 (en) | Modified transposases for improved insertion sequence bias and increased dna input tolerance | |
| EP3157573A4 (en) | Alternative nucleic acid molecules and uses thereof | |
| EP3157572A4 (en) | Alternative nucleic acid molecules and uses thereof | |
| EP3253910A4 (en) | Multiple-emulsion nucleic acid amplification | |
| EP3313989A4 (en) | Modified crispr rna and modified single crispr rna and uses thereof | |
| EP3188763A4 (en) | Methods and compositions for rna-directed target dna modification | |
| EP3095054A4 (en) | Methods and systems for genome analysis | |
| EP3132025A4 (en) | Methods and compositions for modifying genomic dna | |
| EP3238129A4 (en) | Input verification | |
| EP3119895A4 (en) | Genome editing without nucleases | |
| EP3102681A4 (en) | Trifunctional t cell-antigen coupler and methods and uses thereof | |
| EP3132060A4 (en) | Methods and systems for nucleic acid amplification | |
| EP3142680A4 (en) | Lpa-associated protein and rna expression | |
| EP3215913A4 (en) | System and method for linking applications | |
| EP3114315A4 (en) | Geosteering systems and methods thereof | |
| EP3178919A4 (en) | Nucleic acid amplification device | |
| EP3134551A4 (en) | Compositions and methods for enhancing and/or predicting dna amplification | |
| EP3247716A4 (en) | Mir-92 inhibitors and uses thereof | |
| EP3167060A4 (en) | Dna amplification technology | |
| EP3212792A4 (en) | Nucleic acid modifying agents and uses thereof | |
| EP3280698A4 (en) | Reagents and methods for esterification | |
| EP3393525A4 (en) | METHODS AND COMPOSITIONS FOR ASSEMBLING NUCLEIC ACIDS | |
| EP3256592A4 (en) | Compositions and methods for modulating rna | |
| EP3183364A4 (en) | Rna amplification methods | |
| EP3227476A4 (en) | Methods and kits for theranostic applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A16 | Divisional, continuation or continuation in part application filed |
Free format text: ST27 STATUS EVENT CODE: A-0-1-A10-A16-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION RECEIVED - DIVISIONAL Effective date: 20250117 |
|
| D11 | Substantive examination requested |
Free format text: ST27 STATUS EVENT CODE: A-1-1-D10-D11-D117 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: REQUEST FOR EXAMINATION RECEIVED Effective date: 20250117 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 7TH ANNIV.) - STANDARD Year of fee payment: 7 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD Year of fee payment: 3 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 9TH ANNIV.) - STANDARD Year of fee payment: 9 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD Year of fee payment: 5 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 2ND ANNIV.) - STANDARD Year of fee payment: 2 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 8TH ANNIV.) - STANDARD Year of fee payment: 8 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 4TH ANNIV.) - STANDARD Year of fee payment: 4 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD Year of fee payment: 6 Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 10TH ANNIV.) - STANDARD Year of fee payment: 10 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20250117 |
|
| A18 | Application divided or continuation or continuation in part accepted |
Free format text: ST27 STATUS EVENT CODE: A-1-1-A10-A18-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DIVISIONAL REQUIREMENTS DETERMINED COMPLIANT Effective date: 20250204 |
|
| D00 | Search and/or examination requested or commenced |
Free format text: ST27 STATUS EVENT CODE: A-1-1-D10-D00-D118 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: REQUEST FOR EXAMINATION REQUIREMENTS DETERMINED COMPLIANT Effective date: 20250204 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20250204 |
|
| P18 | Priority claim added or amended |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P18-P112 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: PRIORITY DOCUMENT OR RESPONSE DETERMINED COMPLIANT Effective date: 20250206 Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P18-P105 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: PRIORITY CLAIM REQUIREMENTS DETERMINED COMPLIANT Effective date: 20250206 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20250206 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20250506 |
|
| P18 | Priority claim added or amended |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P18-P112 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: PRIORITY DOCUMENT OR RESPONSE DETERMINED COMPLIANT Effective date: 20250922 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20250923 |
|
| P22 | Classification modified |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P22-P110 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CLASSIFICATION MODIFIED Effective date: 20251010 |
|
| A00 | Application filed |
Free format text: ST27 STATUS EVENT CODE: A-1-1-A10-A00-A102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: COMPLIANCE REQUIREMENTS DETERMINED MET Effective date: 20251203 |
|
| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P13-P146 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: BSL VERIFIED - NO DEFECTS Effective date: 20251203 |
|
| Q12 | Application published |
Free format text: ST27 STATUS EVENT CODE: A-1-1-Q10-Q12-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION PUBLISHED (OPEN TO PUBLIC INSPECTION) Effective date: 20260302 |
|
| D11 | Substantive examination requested |
Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D11-D155 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: ALL REQUIREMENTS FOR EXAMINATION DETERMINED COMPLIANT Effective date: 20260309 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 11TH ANNIV.) - STANDARD Year of fee payment: 11 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20260324 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20260324 |