CA3198905A1 - Dna-containing polynucleotides and guides for crispr type v systems, and methods of making and using the same - Google Patents

Dna-containing polynucleotides and guides for crispr type v systems, and methods of making and using the same

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Publication number
CA3198905A1
CA3198905A1 CA3198905A CA3198905A CA3198905A1 CA 3198905 A1 CA3198905 A1 CA 3198905A1 CA 3198905 A CA3198905 A CA 3198905A CA 3198905 A CA3198905 A CA 3198905A CA 3198905 A1 CA3198905 A1 CA 3198905A1
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Canada
Prior art keywords
sequence
cell
car
nucleic acid
less
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Pending
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CA3198905A
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English (en)
French (fr)
Inventor
Paul Daniel Donohoue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caribou Biosciences Inc
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Caribou Biosciences Inc
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Application filed by Caribou Biosciences Inc filed Critical Caribou Biosciences Inc
Publication of CA3198905A1 publication Critical patent/CA3198905A1/en
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/31—Chimeric antigen receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/4214—Receptors for cytokines
    • A61K40/4215—Receptors for tumor necrosis factors [TNF], e.g. lymphotoxin receptor [LTR], CD30
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
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    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
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    • C12N2310/3531—Hydrogen
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    • C12N2510/00—Genetically modified cells

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CA3198905A 2020-10-19 2021-10-18 Dna-containing polynucleotides and guides for crispr type v systems, and methods of making and using the same Pending CA3198905A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063093459P 2020-10-19 2020-10-19
US63/093,459 2020-10-19
US202063127648P 2020-12-18 2020-12-18
US63/127,648 2020-12-18
US202163229870P 2021-08-05 2021-08-05
US63/229,870 2021-08-05
PCT/US2021/055394 WO2022086846A2 (en) 2020-10-19 2021-10-18 Dna-containing polynucleotides and guides for crispr type v systems, and methods of making and using the same

Publications (1)

Publication Number Publication Date
CA3198905A1 true CA3198905A1 (en) 2022-04-28

Family

ID=78819995

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3198905A Pending CA3198905A1 (en) 2020-10-19 2021-10-18 Dna-containing polynucleotides and guides for crispr type v systems, and methods of making and using the same

Country Status (9)

Country Link
US (1) US20250027078A1 (https=)
EP (1) EP4229198A2 (https=)
JP (1) JP7698715B2 (https=)
KR (1) KR20230090337A (https=)
AU (1) AU2021364314C1 (https=)
CA (1) CA3198905A1 (https=)
IL (1) IL302220A (https=)
MX (1) MX2023004505A (https=)
WO (1) WO2022086846A2 (https=)

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CN110494559B (zh) * 2017-03-28 2023-10-31 味之素株式会社 不分化地维持用培养基添加剂
EP4508211A2 (en) * 2022-04-13 2025-02-19 Caribou Biosciences, Inc. Therapeutic applications of crispr type v systems
WO2023225035A2 (en) * 2022-05-16 2023-11-23 Artisan Development Labs, Inc. Compositions and methods for engineering cells
EP4504764A1 (en) 2022-05-26 2025-02-12 Caribou Biosciences, Inc. Cytokine-receptor fusions for immune cell stimulation
WO2024046307A1 (zh) * 2022-08-29 2024-03-07 北京迅识科技有限公司 一种突变的v型crispr酶及其应用
AU2023353989A1 (en) 2022-09-30 2025-02-27 Caribou Biosciences, Inc. Anti-ror1 chimeric antigen receptors (cars), cells expressing the cars and related methods
WO2024102434A1 (en) 2022-11-10 2024-05-16 Senda Biosciences, Inc. Rna compositions comprising lipid nanoparticles or lipid reconstructed natural messenger packs
EP4584283A1 (en) 2022-11-14 2025-07-16 Caribou Biosciences, Inc. Anti-cll-1 chimeric antigen receptors, engineered cells and related methods
AU2024236558A1 (en) 2023-03-15 2025-10-09 Renagade Therapeutics Management Inc. Delivery of gene editing systems and methods of use thereof
EP4704886A1 (en) * 2023-05-01 2026-03-11 Caribou Biosciences, Inc. Treatment of autoimmune diseases with bcma-targeting engineered immune cells
WO2025006880A1 (en) 2023-06-29 2025-01-02 Caribou Biosciences, Inc. Diagnostic and therapeutic methods for use with ror1 antagonists
WO2025024679A1 (en) * 2023-07-26 2025-01-30 Caribou Biosciences, Inc. In vitro validation methods for car-t cell therapies
AU2024335327A1 (en) 2023-09-01 2026-03-26 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025117745A1 (en) 2023-12-01 2025-06-05 Caribou Biosciences, Inc. Methods and compositions for improving precision of endonuclease-mediated genome editing
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents
WO2025231432A1 (en) * 2024-05-03 2025-11-06 Caribou Biosciences, Inc. In vivo gene editing with crispr systems
WO2026024468A1 (en) 2024-07-26 2026-01-29 Caribou Biosciences, Inc. Modular linked chimeric antigen receptors
CN119881333B (zh) * 2025-01-27 2025-11-28 上海市肿瘤研究所 一种dna纳米花级联crispr系统的蛋白标志物检测平台及其构建方法和应用

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US9650617B2 (en) * 2015-01-28 2017-05-16 Pioneer Hi-Bred International. Inc. CRISPR hybrid DNA/RNA polynucleotides and methods of use
EP3423580A1 (en) * 2016-03-04 2019-01-09 Editas Medicine, Inc. Crispr-cpf1-related methods, compositions and components for cancer immunotherapy
CN105912952B (zh) * 2016-05-04 2019-07-23 广州广电运通金融电子股份有限公司 一种基于Linux的注册表服务系统、方法及金融自助设备
WO2017190664A1 (zh) * 2016-05-05 2017-11-09 苏州吉玛基因股份有限公司 化学合成的crRNA和修饰crRNA在CRISPR/Cpf1基因编辑系统中的应用
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WO2018009822A1 (en) * 2016-07-08 2018-01-11 Ohio State Innovation Foundation Modified nucleic acids, hybrid guide rnas, and uses thereof
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WO2022086846A2 (en) 2022-04-28
KR20230090337A (ko) 2023-06-21
JP2023546158A (ja) 2023-11-01
EP4229198A2 (en) 2023-08-23
JP7698715B2 (ja) 2025-06-25
AU2021364314A1 (en) 2023-03-16
WO2022086846A3 (en) 2022-07-21
MX2023004505A (es) 2023-05-10
AU2021364314C1 (en) 2025-02-20
IL302220A (en) 2023-06-01
WO2022086846A9 (en) 2023-06-15
AU2021364314B2 (en) 2024-11-21
US20250027078A1 (en) 2025-01-23

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