CA3138627A1 - Structurally defined sirna-dual variable domain immunoglobulin conjugates - Google Patents

Structurally defined sirna-dual variable domain immunoglobulin conjugates Download PDF

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Publication number
CA3138627A1
CA3138627A1 CA3138627A CA3138627A CA3138627A1 CA 3138627 A1 CA3138627 A1 CA 3138627A1 CA 3138627 A CA3138627 A CA 3138627A CA 3138627 A CA3138627 A CA 3138627A CA 3138627 A1 CA3138627 A1 CA 3138627A1
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CA
Canada
Prior art keywords
conjugate
antisense strand
seq
positions
dsrna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3138627A
Other languages
English (en)
French (fr)
Inventor
Alexander V. KEL'IN
Alex R. NANNA
Christoph Rader
Christopher THEILE
Justin PIERSON
Kevin Fitzgerald
Zhi Xiang Voo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alnylam Pharmaceuticals Inc
University of Florida Research Foundation Inc
Original Assignee
Scripps Research Institute
Alnylam Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute, Alnylam Pharmaceuticals Inc filed Critical Scripps Research Institute
Publication of CA3138627A1 publication Critical patent/CA3138627A1/en
Pending legal-status Critical Current

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    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
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    • C—CHEMISTRY; METALLURGY
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    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
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    • C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
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CA3138627A 2019-06-21 2020-06-18 Structurally defined sirna-dual variable domain immunoglobulin conjugates Pending CA3138627A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962864755P 2019-06-21 2019-06-21
US62/864,755 2019-06-21
PCT/US2020/038475 WO2020257483A1 (en) 2019-06-21 2020-06-18 Structurally defined sirna-dual variable domain immunoglobulin conjugates

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CA3138627A1 true CA3138627A1 (en) 2020-12-24

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CA3138627A Pending CA3138627A1 (en) 2019-06-21 2020-06-18 Structurally defined sirna-dual variable domain immunoglobulin conjugates

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US (1) US20230049529A1 (https=)
EP (1) EP3986475A4 (https=)
JP (2) JP7785543B2 (https=)
AU (1) AU2020295485A1 (https=)
CA (1) CA3138627A1 (https=)
WO (1) WO2020257483A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113512557A (zh) * 2021-04-15 2021-10-19 山西大学 飞蝗脱水抗性基因LmDesi及其dsRNA的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4851940B2 (ja) * 2004-11-19 2012-01-11 宮城県 がんまたは糖尿病治療のための医薬組成物
US20110223665A1 (en) * 2008-07-25 2011-09-15 Alnylam Pharmaceuticals, Inc. ENHANCEMENT OF siRNA SILENCING ACTIVITY USING UNIVERSAL BASES OR MISMATCHES IN THE SENSE STRAND
IL316808A (en) * 2014-08-20 2025-01-01 Alnylam Pharmaceuticals Inc Modified double-stranded RNA materials and their uses
CN108025071B (zh) * 2015-09-17 2022-11-01 斯克利普斯研究院 双重可变结构域免疫缀合物及其用途
CA3049133A1 (en) * 2017-01-18 2018-07-26 Alnylam Pharmaceuticals, Inc. Endosomal cleavable linkers

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CN113512557A (zh) * 2021-04-15 2021-10-19 山西大学 飞蝗脱水抗性基因LmDesi及其dsRNA的应用

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